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General characteristics of Antheraea yamamai silkworm cocoon cultured in Korea (천잠 누에고치의 일반 성상)

  • Lee, Kwang-Gill;Chung, Da-Eun;Kim, Kee-Young;Jo, You-Young;Kim, Hyun-Bok;Kim, Sung-Kuk;Kweon, HaeYong
    • Journal of Sericultural and Entomological Science
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    • v.53 no.1
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    • pp.6-11
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    • 2015
  • Antheraea yamamai silkworm cocoon is considered as a natural biocompatible materials, but can not be used as medical resources due to its difficult processability. In this study, we examined the general characteristics including cocoon shell weight and structural and thermal properties of Antheraea yamamai silkworm cocoon. The cocoon shell weight and thickness of wild silkworm cocoon was 0.528 g and 0.424 mm, respectively. The cocoon has yellow-green color in outside cocoon but white in inner layer cocoon. Amino acid analysis showed that the main amino acid of Antheraea yamamai cocoon is alanine, glycine, serine, aspartic acid, tyrosine and arginine. X-ray diffractometry showed that strong diffraction peaks at $2{\theta}=16.8^{\circ}$, $20.4^{\circ}$, corresponded to ${\beta}$-sheet structure and sharp diffraction peaks at $2{\theta}=15.0^{\circ}$, $24.3^{\circ}$, $30.0^{\circ}$ due to the presence of calcium oxalate on cocoon surface. Antheraea yamamai cocoon was degraded at $370^{\circ}C$. These results might be used as basic information for development of non-textile materials using Anthereae yamamai silkworm cocoon.

Review of Failure Mechanisms on the Semiconductor Devices under Electromagnetic Pulses (고출력전자기파에 의한 반도체부품의 고장메커니즘 고찰)

  • Kim, Dongshin;Koo, Yong-Sung;Kim, Ju-Hee;Kang, Soyeon;Oh, Wonwook;Chan, Sung-Il
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.6
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    • pp.37-43
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    • 2017
  • This review investigates the basic principle of physical interactions and failure mechanisms introduced in the materials and inner parts of semiconducting components under electromagnetic pulses (EMPs). The transfer process of EMPs at the semiconducting component level can be explained based on three layer structures (air, dielectric, and conductor layers). The theoretically absorbed energy can be predicted by the complex reflection coefficient. The main failure mechanisms of semiconductor components are also described based on the Joule heating energy generated by the coupling between materials and the applied EMPs. Breakdown of the P-N junction, burnout of the circuit pattern in the semiconductor chip, and damage to connecting wires between the lead frame and semiconducting chips can result from dielectric heating and eddy current loss due to electric and magnetic fields. To summarize, the EMPs transferred to the semiconductor components interact with the chip material in a semiconductor, and dipolar polarization and ionic conduction happen at the same time. Destruction of the P-N junction can result from excessive reverse voltage. Further EMP research at the semiconducting component level is needed to improve the reliability and susceptibility of electric and electronic systems.

Form and Material Analysis of Yuso Used in Joseon Period Scroll Paintings (조선시대 족자 장황에 사용된 유소 형태 및 재질분석)

  • Jang, Yeonhee;Yun, Eunyoung;Kim, Yein;Park, Jinyoung
    • Conservation Science in Museum
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    • v.17
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    • pp.69-84
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    • 2016
  • Yuso is the term for decorative tassels of a braided string which hangs a scroll painting. This study, drawing on extant research concerning the yuso made for Joseon period portrait scrolls of kings and meritorious retainers, focuses on the yuso created to hang literati portraits. Concretely, It examines yuso of seven portraits in the collection of the National Museum of Korea in order to characterize their appearance and determine their material composition. The study found that most of the yuso are sixteen-strand strings braided into a rounded cross-section(dongdahoe). The seven yuso, of which six are red and one indigo-blue, reflect the popular style associated with Joseon period literati portraits. The yuso for the portrait of Yun Geup(duksu 3503) is made from gilded paper. Analysis showed Fe particles present in a red pigment underlying the gold layer, suggesting the presence of red ochre(seokganju), an iron oxide mineral. The yuso of the portrait of Shin Im(duksu 4846) is used a paper which contains gold as well as traces of Pb, Hg and Ag. The paper in the yuso for the portrait of Yi Seongwon(bongwan 10122) mainly consisted of Ag, indicating silver paper having been used in its fabrication. The inner paper in the yuso of the portrait of Yi Seogu(sinsu 1065) is a leather combined with Ag, Fe, and Br, according to chemical analysis. The FTIR of the leather sample reveals that the spectrum in the fingerprint region is nearly identical to that of sheepskin, indicating the yuso was made from gold-coated sheepskin.

Experimental Studies on Pathogenicities and Developmental Stages of Nosema apis(Zander, 1909) (꿀벌에 기생(寄生)하는 Nosema apis의 병원성(病原性)과 발육단계(發育段階)에 관한 연구(硏究))

  • Kang, Yung Bai;Kim, Dong Sung;Jang, Du Hwan
    • Korean Journal of Veterinary Research
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    • v.16 no.1
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    • pp.11-25
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    • 1976
  • Studies on pathogenicities and developmental stages of Nosema apis (Zander, 1909) were carried out through artificial infection to Nosema free honey bees with various levels of spores isolated from local honey bee colony. The results obtained were summarized as follows: 1. The clinical symptoms were observed as dysentery, enteritis of mid-gut (enlargement and decoloration), crawling posture and shortening of the longevity of worker bees in the rearing honey bee colony inoculated with the spores. 2. Number of spores harvested from laboratory rearing honey bees were progresively increased to 4 weeks after inoculation. The regression equations and coefficients of correlations to various spore levels were as follows in each treatment colony. Colony 1. ($$1,000{\times}10^4spores/ml$$) $$y_{c1}=471{\times}10^{4}x+454{\times}10^4(r=0.65^*$$) Colony 2. ($$500{\times}10^4spores/ml$$) $$y_{c2}=340{\times}10^{4}x+207.8{\times}10^4(r=0.99^{**}$$) Colony 3. ($$100{\times}10^4spores/ml$$) $$y_{c3}=150{\times}10^{4}x+84.2{\times}10^4(r=0.99^{**}$$) Colony 4. ($$10{\times}10^4spores/ml$$) $$y_{c4}=13.8{\times}10^{4}x+13{\times}10^4(r=0.98^{**}$$) 3. Average longevity of worker bees artificially infected with Nosema apis was shortened as 21.7~43.8% compare to the control. (p<.05, p<.01) 4. The spores which were isolated from honey bee colony infected with Nosema disease were ovoid or spherical form, and measured, as a rule, from $4.7{\mu}m$ to $6.1{\mu}m$ (mean $5.3{\mu}m$) in length and from $2.4{\mu}m$ to $3.2{\mu}m$ (mean $2.9{\mu}m$) in width. 5. In the mid-gut of honey bees, the spore was progresively germinated and became trophozoite stage. The trophozoites were grown to meronts and their binary fission were begun. The divided two sporoblasts were developed to the spores which had elastic membrane. The new spores were shed in excreta of honey bees 10~15 day after inoculation at $25{\pm}2$ centigrade. 6. The ultrastructure of spore membrane consisted of three layers, such as, outer, middle and inner layer. The sporoplasm consisting lamellar structure occupied only anterior part of the spore and was often extended to posterior direction where definite vacuoles and a polar filament was able to detect.

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Effects of The Knife-incising and Kerfing Pretreatment on Moisture Content and Surface Check Occurrence of Douglas-fir Heavy Timber (배할 및 자상-인사이징 전처리가 Douglas-fir 중목구조부재의 함수율과 재면할렬 발생에 미치는 영향)

  • Lee, Chang-Jin;Lee, Nam-Ho;Oh, Seung-Won
    • Journal of the Korean Wood Science and Technology
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    • v.44 no.3
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    • pp.302-314
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    • 2016
  • This study was carried out to confirm the effects of the knife-incising and kerfing pretreatment on the moisture content and surface check occurrence of Douglas-fir heavy timber. In the case of moisture content, the moisture gradient that formed in the inner part of the specimen was observed in all specimens. In addition, the moisture gradient was formed differently from the surface layer to 72 mm depending on the pretreatment conditions, and it was formed gradually in the kerfing than the knife-incising. In addition, it was found that the solid volume should be considered when measuring the average moisture content. In the case of surface check, the surface check occurrence was reduced in the knife-incising, kerfing, and the combination of knife-incising and kerfing than the control. In particular, the kerfing treatment was shown that the expansion of surface check width was effectively suppressed. In the case of knife-incising treatment, although the surface check was less than the control, the preventive effect on the surface check occurrence did not reach the level of the kerfing treatment.

Field Emission Properties of Multiwalled Carbon Nanotubes Synthesized by Pin-to-Plate Type Atmospheric Pressure Plasma Enhanced Chemical Vapor Deposition (Pin-to-plate Type 대기압 PECVD 방법을 이용해 성장된 다중벽 탄소나노튜브의 전계방출 특성연구)

  • Park Jae-Beom;Kyung Se-Jin;Yeom Geun-Young
    • Journal of the Korean Vacuum Society
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    • v.15 no.4
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    • pp.374-379
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    • 2006
  • In this study, carbon nanotubes (CNTs) were grown on glass substrates coated with Ni/Cr by an atmospheric pressure plasma enhanced chemical vapor deposition(AP-PECVD) and their structural and electrical characteristics were investigated as a possible application to the field emitter of field emission display (FED) devices. The substrate temperature ($400{\sim}500^{\circ}C$) were varied and the grown CNTs were multi wall CNTs (at $500^{\circ}C$, 15 - 20 layers of graphene sheets, distance of each layer : 0.3nm, inner diameter: 10 - 15nm, outer diameter: 30 - 40nm). The ratio of defective carbon peak to graphite carbon peak of the CNTs grown at $500^{\circ}C$ (C measured by fourier transform(FT)-Raman was 0.772 $I_D / I_G$ ratio. When field emission properties were measured, the turn-on field was $2.92V/{\mu}m$ and the emission field at $1mA/cm^2$ was $5.325V /{\mu}m$.

Preparation of Asymmetric Folyethersulfone Hollow Fiber Membranes for Flue Gas Separation (온실기체 분리용 폴리이서설폰 비대칭 중공사 막의 제조)

  • Kim Jeong-Hoon;Sohn Woo-Ik;Choi Seung-Hak;Lee Soo-Bok
    • Membrane Journal
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    • v.15 no.2
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    • pp.147-156
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    • 2005
  • It is well-known that polyethersulfone (PES) has high $CO_2$ selectivity over $N_2\;(or\;CH_4)$ and excellent pressure resistance of $CO_2$ plasticization among muy commercialized engineering plastics[1-4]. Asymmetric PES hollow fiber membranes for flue gas separation were developed by dry-wet spinning technique. The dope solution consists of PES, NMP and acetone. Water and water/NMP mixtures are used in outer and inner coagulants, respectively. Gas permeation rate (i.e., permeance) and $CO_2/N_2$ selectivity were measured with pure gas, respectively and the micro-structure of hollow fiber membranes was characterized by scanning electron microscopy. The effects of polymer concentration, ratio of NMP to acetone, length of air gap, evaporation condition and silicone coating were investigated on the $CO_2/N_2$ separation properties of the hollow fibers. Optimized PES hollow fiber membranes exhibited high permeance of $25\~50$ GPU and $CO_2/N_2$ selectivity of $30\~40$ at room temperature and have the apparent skin layer thickness of about $0.1\;{\mu}m$. The developed PES hollow fiber membranes, would be a good candidate suitable for the flue gas separation process.

Characteristics of artificial lightweight fine aggregates manufactured by using a vertical fluidizing furnace (수직형 유동층로에서 제조된 인공경량 세골재의 특성)

  • Kang, Seung-Gu
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.19 no.1
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    • pp.54-59
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    • 2009
  • It was difficult for the existing rotary kiln to fabricate the fine aggregates under 3 mm due to the sticking phenomenon between specimens. In this study, the vertical type fluidizing furnace was designed and manufactured by which the lightweight fine aggregates of specific gravity $1.1{\sim}1.7$, water absorption $11{\sim}19%$ could be fabricated from the green body of clay: stone sludge: spent bleaching clay = 60 : 30 : 10 (wt%) without sticking-together happening. The minimum sintering temperature for bloating of aggregates was $1130^{\circ}C$. The specimens sintered over $1140^{\circ}C$ showed the typical bloating characteristics of lightweight aggregates and an inner layer was discovered due to widened cracks on a surface. But the crack on a surface did not propagate into a black core area so had no effect on a water absorption of aggregates. The sintering temperature made the thickness of shell and the black core area thin and expanded respectively but the sintering time did not affect the microsturcture of aggregates. The water absorption of aggregates decreased with increasing temperature owing to increased amount of liquid formed on a surface. Also sintering time affected a lot on a water absorption because it takes a time to form a liquid, which change the open pores to closed pores by blocking.

A Study on the Prediction System of Block Matching Rework Time (블록 정합 재작업 시수 예측 시스템에 관한 연구)

  • Jang, Moon-Seuk;Ruy, Won-Sun;Park, Chang-Kyu;Kim, Deok-Eun
    • Journal of the Society of Naval Architects of Korea
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    • v.55 no.1
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    • pp.66-74
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    • 2018
  • In order to evaluate the precision degree of the blocks on the dock, the shipyards recently started to use the point cloud approaches using the 3D scanners. However, they hesitate to use it due to the limited time, cost, and elaborative effects for the post-works. Although it is somewhat traditional instead, they have still used the electro-optical wave devices which have a characteristic of having less dense point set (usually 1 point per meter) around the contact section of two blocks. This paper tried to expand the usage of point sets. Our approach can estimate the rework time to weld between the Pre-Erected(PE) Block and Erected(ER) block as well as the precision of block construction. In detail, two algorithms were applied to increase the efficiency of estimation process. The first one is K-mean clustering algorithm which is used to separate only the related contact point set from others not related with welding sections. The second one is the Concave hull algorithm which also separates the inner point of the contact section used for the delayed outfitting and stiffeners section, and constructs the concave outline of contact section as the primary objects to estimate the rework time of welding. The main purpose of this paper is that the rework cost for welding is able to be obtained easily and precisely with the defective point set. The point set on the blocks' outline are challenging to get the approximated mathematical curves, owing to the lots of orthogonal parts and lack of number of point. To solve this problems we compared the Radial based function-Multi-Layer(RBF-ML) and Akima interpolation method. Collecting the proposed methods, the paper suggested the noble point matching method for minimizing the rework time of block-welding on the dock, differently the previous approach which had paid the attention of only the degree of accuracy.

Establishment of Human Embryonic Stem Cells Derived from Frozen-Thawed Blastocysts (동결-융해된 인간 배반포기 배 유래의 배아 간(幹) 세포 배양)

  • Kim, Eun-Young;Nam, Hwa-Kyung;Lee, Keum-Sil;Park, Sae-Young;Park, Eun-Mi;Yoon, Ji-Yeon;Heo, Young-Tae;Cho, Hyun-Jung;Park, Se-Pill;Chung, Kil-Saeng;Lim, Jin-Ho
    • Clinical and Experimental Reproductive Medicine
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    • v.28 no.1
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    • pp.33-40
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    • 2001
  • Objective: This study was to establish the human embryonic stem (ES) cells derived from frozen-thawed blastocyst stage embryo that were destined to be discarded after five years in routine human IVF-ET program. Methods: Frozen-thawed and survived human blastocysts were treated by immunosurgery, and recovered ICM cells were cultured onto STO feeder cell layer and ICM colony was subcultured by mechanical dissociation into clumps. To identify ES cell, alkaline phosphatase staining and expression of Oct4 in replated ICM colonies were examined. Also, to examine the possibility of ES cell differentiation, retinoic acid (RA), basic fibroblast growth factor (b-FGF), nerve growth factor (NGF) were added in culture medium. In addition, to classify the specific cell type, differentiated cells were stained by indirect immunocytochemistry. Results: One ICM colony recovered from frozen-thawed six blastocysts was subcultured, continuously replated during 40 passage culture duration without differentiation. Subcultured colonies were strong positively stained by alkaline phophatase. When the expression of Oct4 in cultured ES colony was examined, Oct4b type is more clearly indicated than Oct4a one although there was not detected in embryoid body or differentiated cells. In differentiated cardiomyocytes from ES colony, cells were beaten regularly (60 times/min). In differentiated neural cells from ES colony, neurofilament (NF) 200 kDa protein, microtubule associated protein (MAP) 2 and ${\beta}$-tubulin of specific marker in neurons, glial fibrillary acidic protein (GFAP) of specific marker in astrocytes and galactocelebrocide (GalC) of specific marker in oligodendrocytes were confirmed by indirect immunocytochemistry. Also, muscle cells were detected by indirect immunocytochemistry. In addition, ES colonies can be successfully cryopreserved. Conclusion: This study suggested that establishment of human ES cells can be successfully derived from frozen-thawed blastocysts that were destined to be discarded, and obtained specific cell types (cardiomyocytes, neurons and muscle cells) through the in vitro differentiation procedures of ES cells.

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