• 한국물환경학회지
• /
• 제22권2호
• /
• pp.300-307
• /
• 2006

The combined SHARON (Single reactor system for High ammonium Removal Over Nitrite)-ANAMMOX (Anaerobic ammonium oxidation) reactor were operated in mesophilic condition ($35^{\circ}C$). In this study, microbial granulation and characteristics of SHARON and ANAMMOX sludges were investigated using settling test, Scanning Electron Microscopy (SEM) and Fluorescence In Situ Hybridization (FISH). In SHARON reactor, Aerobic granulation with diameter of 1.5~2.5 mm was accomplished but aerobic granulation was weaker than anaerobic granular sludge. Initial seed sludge of ANAMMOX reactor was used as attached media for biofilm growth. ANAMMOX sludge was more compact and rounder rather than seed sludge. Though ANAMMOX sludge has high activity, it has lower settling ability than the seed granule. The color of ANAMMOX sludge was changed from dark to redish brown granular with diameter of 1~2 mm. In FISH of ANAMMOX sludge, high fraction of Candidatus B. stuttgartiensis which paid great role of nitrogen conversion was detected. Also, FISH results reveals that ANAMMOX bacteria inhabit at inner parts near surface, having advantages in utilization of substrates and protection from oxygen inhibition.

• 한국작물학회지
• /
• 제61권1호
• /
• pp.57-63
• /
• 2016

콩 재배 및 이용확대를 위한 기초자료를 얻기 위해 발아과정 중 기능성 성분인 soyasaponin의 함량 변화를 조사하였다. 1. Soyasaponin I과 soyasaponin II의 함량은 품종에 따라 차이가 있었으나 발아과정 중 떡잎부위에서 함량이 10배 이상 증가하였고, soyasaponin I은 배축에서도 종자 당 이 $140{\sim}360{\mu}g$ 수준의 함량을 나타내었다. 2. Soyasaponin III 및 soyasaponin V의 함량은 발아를 통해 증가하는 경향이었고, soyasaponin V의 경우 배축에서 그 함량이 급격히 증가하는 것으로 나타났다.

• 한국정보처리학회:학술대회논문집
• /
• 한국정보처리학회 2003년도 추계학술발표논문집 (상)
• /
• pp.283-286
• /
• 2003

군집화 시 일반적으로 개별 클래스(class) 혹은 카테고리(category) 당 하나의 군집이 형성되는 결과가 선호된다. 하지만 데이터가 비정형적인 분포를 따르는 경우에는 하나의 군집으로 개별 클래스를 온전히 표현하는 것이 불가능하거나 오히려 부자연스러운 경우가 발생할 수 있다. 본 논문에서는 예제의 클래스를 알고 있는 즉, 레이블(label)된 예제들을 그렇지 않은(unlabeled) 예제들과 함께 활용하여 군집화하는 제약된 K-means (constrained K-means) 알고리즘을 위하여 보다 자연스러운 형태의 군집이 형성될 수 있도록 초기 씨드(seed, 씨앗)를 생성하는 방안을 제안한다. 레이블된 예제들을 계층적으로 군집화하면 다양한 단계에서 제약된 K-means를 위한 씨드집합을 생성할 수 있다. 본 연구에서는 각 단계의 씨드집합을 기반으로 형성된 군집결과간의 변화정도를 측정하여 가장 적절한 것으로 추정되는 씨드집합을 선정하였다. 제안한 방안을 문서 군집화 문제에 적용하여 실험한 결과 개별 클래스마다 하나의 군집을 가정하는 경우보다 더 나은 군집을 형성할 수 있음을 확인하였다.

• 대한안전경영과학회지
• /
• 제12권1호
• /
• pp.97-104
• /
• 2010

Expanded muti-source Weber problem (EWP), which introduced in this paper, is a reverse logistics network design problem to minimize the total transportation cost from customers thorough regional center to central center. Decision factor of EWP are the locations of regional centers and a central center. We introduce a GRASP heuristics for the EWP. In the suggested GRASP, an expanded iterative location allocation method (EILA) is introduced based on the Cooper's iterative location allocation method[3]. For the initial solution of GRASP, allocation first seed (AFSeed) and location first seed (LFSeed) are developed. The computational experiment for the objective value shows that the LFSeed is better than the AFSeed. Also the calculating time of the LFSeed is better than that of the AFSeed.

• 아시안잔디학회지
• /
• 제12권4호
• /
• pp.195-202
• /
• 1998

The development of a protocol for high efficiency of embryogenic callus separation, maintenance and plant regeneration from the seeds of zoysiagrass cv. Zenith was studied. Embryogenic callus ratio is absolutely determined by genotype, but by adding high concentration of copper into medium, changing light condition and maintaining callus on initial induction medium for 8∼10 weeks, embryogenic callus can be easily distinguished and its growth can be promoted. There were significant differences among selected callus lines (each from one seed) according to their growth rates and regeneration percentages. Callus pre-treatment with activated charcoal inhibited callus growth, increased the level of precocious germination during culture and promoted shoot cluster formation after transfer to regeneration medium. For long-term callus maintenance, N6AA medium was better than MS medium, because the former inhibited non-embryogenic callus formation and kept vigor of embryogenic callus. The best callus lines Z-(5) has been successfully used for transformation and somaclonal variation selection in our laboratory.

• 한국연초학회지
• /
• 제21권2호
• /
• pp.152-159
• /
• 1999

A CAB cDNA clone(pKGCAB) encoding the light harvesting chlorophyll a/b binding protein of the semi-shade plant, Korean ginseng(Panax ginseng C. A. Meyer) was isolated by the one-way path random sequencing of ginseng cDNA library clones and transgenic tobacco plants(Nicotiana tabacum NC82) were produced by the transformation of this ginseng CAB gene in use of Agrobacterium tumefaciens LBA4404. The CAB gene showed type 1 structure of LHCP-II, 84% similarity in nucleotide sequence and 92% in amino acid sequence to that of Nicotiana tabacum CAB40, respectively. Seed germination and initial growth of the transgenic tobacco plants transformed with the cDNA fragment were accelerated under low light intensity compared with those of normal tobacco plant, that may result from the higher light sensitivity of the transgenic plants than that of the normal.

• BMB Reports
• /
• 제37권6호
• /
• pp.715-719
• /
• 2004

The fate of Telfairia occidentalis seed Agglutinin, TOA, has been monitored during germination. While the level of the Agglutinin in the cotyledons decreased sharply in the first three days to about half of the initial level, it stabilises at this level for the following twelve days. In this interval, Agglutinin activity becomes manifest in the radicle on the fourth day, peaking on the fifth and decreasing rapidly thereafter. In the plumule, the lectin activity becomes manifest on the sixth day, peaks on the seventh and decreases rapidly thereafter. No lectin activity is detectable in any plant tissue including the rump of the cotyledons twenty-seven days after germination. The implications of these observations on the possible role of lectins in plants are discussed.

• Journal of Plant Biology
• /
• 제23권1호
• /
• pp.11-15
• /
• 1980

ATP contents of soybean(Glycine max Merrill) and mungbean (Phaseolus radiatus L.) seeds being germinated with Hoagland solution at $30^{\circ}C$ for 6 days were determined. In pregerminated seed, ATP contents in soybean and mumgbean were 11.4 and 63.0$\mu$g/g fresh seed respectively. During germination, the highest ATP content of soybean seeds was 550% of initial content on 2nd day and that of mungbean was 480% on 1st day after germination. ATP content in cotyledon of soybean and mungbean were increased up to 4th and 1st day after germination respectively, thereafter both were decreased, but those in the root, including the hypocotyl, of both seeds were continuously increased with germination progress.

• Journal of Animal Science and Technology
• /
• 제64권4호
• /
• pp.696-706
• /
• 2022

This study was conducted to evaluate the effects of dietary supplementation of Silybum marianum seed (SMS) extract on the growth performance, nutrient digestibility, fecal noxious gas emission, and hematology parameters in weaned piglets. A total of 120, 21-day-old weaned piglets ([Yorkshire × Landrace] × Duroc) were randomly assigned to 3 groups based on the average initial body weight (6.46 ± 0.45 kg). There were 8 replicate pens per treatment and 5 pigs (mixed sex) per pen. The experimental period was 42 days. Dietary groups included a basal diet, and a basal diet supplemented with 0.05% or 0.10% SMS extract. Feeding weaned piglets with SMS extract containing diet significantly increased average daily gain and average daily feed intake. Additionally, the supplementation of SMS extract had no significant effects on nutrient digestibility, serum hematology, and fecal noxious gas emission parameters. We considered that the supplementation of SMS extract had positive effects on the voluntary feed intake in weaned piglets, thus improving growth performance.

• 한국잡초학회지
• /
• 제8권1호
• /
• pp.15-22
• /
• 1988

본(本) 연구(硏究)는 큰달맞이꽃종자(種子)의 발아(發芽)에 미치는 광(光)의 생리적작용(生理的作用)을 파악(把握)하기 위하여 발아요인(發芽要因)들(광질(光質), 저온(低溫), 변온(變溫), 수분(水分), 산십(酸十), 종피(種皮) 등) 상호작용(相互作用)을 광(光)과 관련(關聯)하여 검토(檢討)하였다. 광(光)은 혐기조건(嫌氣條件) 및 낮은 water potential (-18bars) 하(下)에서도 발아초기(發芽初期) 단계(段階)를 유기(誘起)시키며 종피(種皮)보다는 비(飛)의 활력(活力)에 영향을 미쳐 발아율(發芽率)을 증가(增加)시켰다. 이러한 광(光)의 효과(效果)중 청색광(靑色光)처리에 의해서는 억제(抑制)되었다. 이는 가역적(可逆的) 반응(反應)을 보였으며 그 정도(程度)는 광(光)의 세기가 높고, 조사기간(照射期間)이 길수록 높은 경향(傾向)이었다. 저온(低溫), 변온(變溫), 종피연화처리(種皮軟化處理) 및 광(光) 등은 발아유기(發芽誘起)에 정(正)의 상호작용(相互作用)을 보였으나 단색광(單色光) 처리(處理)에서는 저온(低溫)의 발아유기효과(發芽誘起效果)를 오히려 저해(沮害)하는 경향(傾向)이었다. 기타 pH 용액(溶液) 및 $GA_3$ 용액(溶液)은 광(光) 및 암발아(暗發芽)와는 무관(無關)하였다.