• 동의생리병리학회지
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• 제16권2호
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• pp.239-244
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• 2002

Okbyungpoongsan-Gami (OG) has been used for the treatment of excessive sweating with general weakness and allergic rhinitis recently. Anal therapy is another way of taking Oriental medicine. It is an important pathway but not available in common clinical situation. This experiment was performed in order to study the inhibitory effect of immediate-type allergic reaction of OG by anal therapy. In addition, the experiment was practised by 1 H-NMR spectroscopy to examine molecular structure of OG. The results were obtained as follows : OG concentration-dependently inhibited compound 48/80- induced immediate type systemic allergic reaction with concentrations of 0.01-1.0g/kg by anal administration 1 h before injection of compound 48/80. OG also concentration-dependently inhibited compound 48/80- induced ear swelling response with concentrations of 0.01-1g/kg by anal administration 1h before injection of compound 48/80. OG also inhibited the passive cutaneous anaphylaxis activated by anti-dinitrophenyl (DNP) IgE antibody concentration- dependently at concentrations ranging from 0.01 to 1g/kg. When OG was pretreated at concentrations ranging from 0.01 to 1g/ℓ, the histamine release from the rat peritoneal mast cells induced by compound 48/80 was reduced in a concentration-dependent manner. OG (0.1-1g/ℓ) had a significant inhibitory effect on histamine release from IgE-induced activated mast cells. OG is seen to be a biochemical compound certainly by 1 H-NMR spectroscopy According to above results, anal therapy of OG may be beneficial in the treatment of systemic and local immediate-type allergic reactions by inhibition of histamine release from mast cells.

• 생명과학회지
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• 제21권7호
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• pp.976-984
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• 2011

감잎으로부터 tyrosinase 억제물질의 추출을 위하여 60% ethanol을 사용하여 추출한 경우 추출물의 phenol성물질 함량이 21.91 mg/g로 가장 높았고, tyrosinase 억제효과도 37%의 억제율로 가장 높게 나타났다. 감잎 추출물로부터 tyrosinase 저해 활성 물질을 정제하기 위하여 Sephadex LH-20 column과 MCI-gel CHP-20 column을 이용하여 gradient로 용출한 결과 tyrosinase 저해 활성을 가지는 2가지 순수한 compound를 정제하였으며, 정제된 2가지 compound를 구조 동정한 결과, (+)-gallocatechin과 prodelphinidin B-3이었다. 정제물의 tyrosinase 저해활성을 측정한 결과 (+)-gallocatechin과 prodelphinidin B-3는 29.5, 40.2%의 저해활성을 나타내었으며, melanoma 세포에서의 멜라닌 생합성 저해효과를 측정한 결과 (+)-gallocatechin과 prodelphinidin B-3는 각각 32.5, 46.7%의 저해활성을 나타내어 멜라닌생성억제효과가 매우 뛰어남을 알 수 있었다. 정제된 미백물질을 이용하여 제조한 essence 제품의 안정성을 살펴본 결과, 미백 essence제품의 pH는 10,000 ppm의 농도에서 4.90~4.95였으며, 점도는 23,000~26,000 cP로 60일 동안의 저장기간 동안 큰 변화를 나타내지 않았다. 일반적 보존 시험인 온도에 따른 안정성과 광안정성(인공광, 자연광) 검사에서도 안정하였으며, 특수, 가혹 보존 시험인 온도순환에 따른 안정성 검사에서도 상의 분리와 변색, 변취 없이 모두 안정함을 확인할 수 있었다.

• KSBB Journal
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• 제26권6호
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• pp.513-516
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• 2011

The occurrence of dental caries is mainly associated with oral pathogens, especially cariogenic Streptococcus mutans. The aim of this study was to isolate and characterize lactic acid bacterium showing inhibitory activity against cariogenic Streptococcus mutans. As results, an isolate with strong inhibitory activity was obtained from Kimchi and it was identified as Lactobacillus sakei by API and 16S rRNA gene analyses. This strain secreted an inhibitory compound in cell growth medium and the activity of the compound was completely disappeared by proteinase K revealing the fact that the compound is proteinous substance, bacteriocin. Optimal culture condition for bacteriocin production by Lb. sakei K-7 was at pH 7.5 and $37^{\circ}C$ for 18 h. Oral administration of this isolate may give anticariogenic and probiotic effects on hosts.

• Journal of Applied Biological Chemistry
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• 제58권1호
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• pp.5-8
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• 2015

This study was to isolate an active component of the chloroform fraction from the methanol extract of Ruta chalepensis leaves and to measure inhibitory effects against ${\alpha}$-glucosidase or ${\alpha}$-amylase. The inhibitory compound of R. chalepensis leaves was isolated using chromatographic methods and identified as quinoline. Quinoline and its structurally related derivatives were tested for their inhibitory activities by evaluating the $IC_{50}$ values against ${\alpha}$-amylase or ${\alpha}$-glucosidase and were compared with that of acarbose. Based on the $IC_{50}$ values, quinazoline exhibited the greatest inhibitory activity ($20.5{\mu}g/mL$), followed by acarbose ($66.5{\mu}g/mL$), and quinoline ($80.3{\mu}g/mL$) against ${\alpha}$-glucosidase. In case of ${\alpha}$-amylase, quinazoline had potent inhibitory activity, followed by quinoline ($179.5{\mu}g/mL$) and acarbose ($180.6{\mu}g/mL$). These results indicate that R. chalepensis extract, quinoline, and quinazoline could be useful for inhibiting ${\alpha}$-glucosidase or ${\alpha}$-amylase.

• Journal of Microbiology and Biotechnology
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• 제19권7호
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• pp.681-684
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• 2009

This study was carried out to evaluate the inhibitory effect of Flammulina velutipes extracts on tyrosinase activity and to identify its biologically active component. The ethyl acetate and n-butanol extracts showed potent tyrosinase inhibitory activities. Subsequently, fractions of the n-butanol extract showed only a partial tyrosinase inhibitory activity. The most active compound of tyrosinase inhibitory activity was identified from the ethyl acetate extract as 1',3'-dilinolenoyl-2'-linoleoylglycerol (LnLLn) by comparing its mass, $^1H-$, and $^{13}C-NMR$spectral data with those previously reported in the literature. LnLLn showed tyrosinase inhibitory activity with an $IC_{50}$value of 16.1 ${\mu}g/ml$. These results suggest that the ethyl acetate extract of F. velutipes could be applicable for the development of a new whitening agent.

• 한국자원식물학회지
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• 제11권
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• pp.89-109
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• 1998

Screening of new antioxidants form oriental medicines resulted in the isolation of a new antioxidative compound and eight known compounds from the stem bark of Kalopanax pictus. On the basis of various spectrosopic studies, the structure of the new compound was determined to be 4-rhamnose-3,5-dimethoxybenzoic acid methly ester. Other known compounds were identified as ferulic acid, 4,5,6,-trihydroxyflavanone, 2', 4',4' -trihydroxychalcone, caffeic acid, coniferyl alcohol, syringin, 1,3-di-O-caffeoylquinic acid. These compounds showed lipid peroxidation inhibitory acitivity in rat liver microsomes and free radical scavenging acitivity.

• 약학회지
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• 제29권5호
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• pp.253-259
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• 1985

In order to search a more active and safer compound, piperine derivatives with substituents in piperidine residue were synthesized and evaluated on CNS depressant activity. N-Piperoyl-2-methylpiperidine (I) and N-piperoyl-3-methylpiperidine (II) were potent in strychnine-induced convulsion. Compound I and N-piperoyl-3-hydroxypiperidine (IX) exhibited a potent inhibitory effect againt pentetrazoleinduced convulsion and a significant prolongation effect of hexobarbital-induced sleeping time. The hydroxy derivatives were more toxic than the methyl derivatives.

• 생약학회지
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• 제29권1호
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• pp.44-47
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• 1998

From the 60% aqueous methanolic fraction of Moutan Cortex Radicis two hyaluronidase inhibitors were isolated and their structures were elucidated by spectroscopic methods. Their structures were identified as paeoniflorin (com-pound I) and oxypaeoniflorin (compound II). Compound I and II exhibited hyaluronidase inhibitory activities with $IC_{50}$ of 1.71 and 1.73mM, respectively.

• Archives of Pharmacal Research
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• 제27권6호
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• pp.624-627
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• 2004

A bile acid derivative, methyl chalate （1）, was isolated from EtOAc extract of the fungus Rhizopus oryzae as a cholesterol biosynthesis inhibitor. It showed moderate inhibitory activity on cholesterol biosynthesis in human Chang liver cells. Compound 1 exhibited inhibitory effect on the later step of cholesterol biosynthesis, indicating that its action mode is different from that of statins that act on the HMG-CoA reductase.

• Archives of Pharmacal Research
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• 제22권1호
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• pp.75-77
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• 1999

Two diterpenoid compounds, agastanol (1) and agastaquinone (2), were isolated from the roots of Agastache rugosa (Labiatae). Compound 1 and 2 showed significant inhibitory effects against human immunodeficiency virus type 1 (HIV-1) protease activity with $IC_{50}$ values of 360 and $87{\mu}M$, respectively.