Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation of 1',3'-Dilinolenoyl-2'-Linoleoylglycerol with Tyrosinase Inhibitory Activity from Flammulina velutipes

  • Jang, Se-Gul (Department of Bioindustry and Technology, Sangji University) ;
  • Jeon, Kyung-Su (Department of Bioindustry and Technology, Sangji University) ;
  • Lee, Eun-Hee (Department of Bioindustry and Technology, Sangji University) ;
  • Kong, Won-Sik (Applied Microbiology Division, National Institute of Agricultural Science and Technology, R.D.A.) ;
  • Cho, Jae-Yong (Department of Bioindustry and Technology, Sangji University, Division of Animal Science and Biotechnology, Sangji University)
  • Published : 2009.07.31
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Abstract

This study was carried out to evaluate the inhibitory effect of Flammulina velutipes extracts on tyrosinase activity and to identify its biologically active component. The ethyl acetate and n-butanol extracts showed potent tyrosinase inhibitory activities. Subsequently, fractions of the n-butanol extract showed only a partial tyrosinase inhibitory activity. The most active compound of tyrosinase inhibitory activity was identified from the ethyl acetate extract as 1',3'-dilinolenoyl-2'-linoleoylglycerol (LnLLn) by comparing its mass, $^1H-$, and $^{13}C-NMR$spectral data with those previously reported in the literature. LnLLn showed tyrosinase inhibitory activity with an $IC_{50}$value of 16.1 ${\mu}g/ml$. These results suggest that the ethyl acetate extract of F. velutipes could be applicable for the development of a new whitening agent.

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