• Maxillofacial Plastic and Reconstructive Surgery
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• v.19 no.4
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• pp.414-434
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• 1997

Though many genetic and epigenetic alterations have been identified in hamster oral carcinogenesis model, there is no information about the possible role of transforming growth factor related with oral cancer. The purpose of this paper was to find the expression patterns of transforming growth factor alpha and beta during the stages of complete oral carcinogenesis model in hamster. 0.5% 9, 10-dimethyl-1, 2-benzanthracene(DMBA) in mineral oil was topically applied to the buccal pouch of 75 hamster three times a week during the experimental periods. The experimental animals were subdivided into two groups of control and experiment. Only the mineral oil was applied to the control group. 0.5% DMBA in mineral oil was applied to the experimental groups of 6, 8, 10, 12, 14, 16, 18 and 20 weeks. The expression of the $TGF-{\alpha}$ and $TGF-{\beta}$ protein were evaluated by the distribution and intensity of positive cells during the carcinogenesis using the immunohistochemical study. The following results were obtained ; 1. The buccal pouch epithelium of hamster was histologically changed to the dysplasia at 6, 8, 10 weeks, carcinoma in situ at 12 weeks, and squamous cell carcinoma at 14 weeks. 2. The expression of the $TGF-{\alpha}$ was restricted to the parabasal and basal layers of the normal and dysplastic mucosa, but those positive cells were extended to the spinous layers of the epithelium in the carcinoma. 3. The degree of $TGF-{\alpha}$ expression was markedly decreased in the carcinoma at 16, 18, 20. The strong positive staining in the center of cancer islands and weak positive staining in periphery of tumor were seen at the stage of squamous cell carcinoma. 4. The positive index of the $TGF-{\alpha}$ had a tendency to increase with DMBA- applied time. There was a statistically significant difference between 12, 18, 20 experimental group and control group (p<0.05). 5. The expression of the $TGF-{\beta}$ was shown at the cytoplasm of all control and experimental groups, and the parabasal and basal layers of the normal and dyslastic mucosa, but it was shown at the basal layers of the epithelium in the carcinoma. 6. $TGF-{\beta}$ was expressed diffusely at 16, 18, 20 experimental group. The strong positive staining in the center of cancer islands and positive staining in periphery of tumor were seen at the stage of squamous cell carcinoma. From the above findings, the expression of $TGF-{\alpha}$ and ${\beta}$ in oral carcinogenesis model seems to have two formal stages, the first being an overexpression step as reaction to uncontrolled growth and the second being one in which external protein accumulate in the surrounding stroma and intracytoplasm. Overexpression of $TGF-{\alpha}$ and ${\beta}$ may have important cooperative roles for the promotion of cancer and factor of prognosis.

• Journal of Life Science
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• v.25 no.2
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• pp.223-230
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• 2015

Regulator of calcineurin 1 (RCAN1) is an endogenous calcineurin inhibitor that plays an important role in the pathogenesis of diseases related to the calcineurin-NFATc1 signaling pathway. The RCAN1-4 isoform is subject to NFATc1-dependent regulation. During receptor activator of nuclear factor kappa-B ligand (RANKL)-stimulated osteoclastogenesis, the calcineurin-NFATc1 pathway is critical. Because there is little information available on the role of RCAN1 in osteoclast differentiation, this study investigated whether changes in RCAN1 expression are related to the calcineurin-NFATc1 pathway and osteoclast differentiation. Mouse bone marrow monocytes (BMMs) were treated with 50 ng/ml of RANKL and M-CSF. Expression levels of NFATc1, calcineurin, and RCAN1 isoforms were determined using RT-PCR and Western blotting. Osteoclast differentiation was examined using tartrate-resistent acid phosphatase (TRAP) staining. To evaluate the effect of RCAN1 overexpression on osteoclastogenesis, cells were transfected with a mouse RCAN1-4 cDNA plasmid. After RANKL stimulation of BMMs, expression of NFATc1 and RCAN1 was increased at the mRNA and protein level, while calcineurin expression was unchanged. When the RCAN1-4 gene construct was transfected, the expression of RCAN1 protein was not increased despite several-fold increases in RCAN1-4 mRNA expression. Regardless of RANKL stimulation, over-expression of RCAN1-4 tended to reduce NFATc1 expression and knock-down of RCAN1 increase it. While BMMs transfected with the RCAN1-4 vector were differentiated into distinct osteoclasts, their phenotypes did not vary from those of mock controls. These results suggest that RCAN1 has a limited effect on the calcineurin-NFATc1 pathway during RANKL-stimulated osteoclast differentiation.

• Korean Journal of Childcare and Education
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• v.10 no.2
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• pp.5-19
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• 2014

The purpose of this study was to examine the effects of pre-service early childhood teachers' self-expression and stress coping style on their human nature. More specifically, this study attempted to investigate how teachers' human nature, self-expression and stress coping style are correlated with another and determine how much human nature is influenced by the other variables, ultimately providing basic information necessary for improving the humanity of pre-service preschool teachers. Findings of the study can be summarized as follows. First, correlations among pre-service early childhood teachers' self-expression, stress coping style and human nature were investigated to find that those teachers' human nature and self-expression have close relationships with each other and that the two variables are significantly related with their stress coping style. Second, the influences of pre-service preschool teachers' self-expression and stress coping style on their human nature were analyzed to find that the human nature of those teachers as a whole and some sub-areas of the variables like positive self-concept and human creativity have significant relationships with their self-expression and stress coping style. Finally, based on these findings, this study discussed how to establish and manage a curriculum that helps to improve the human nature of pre-service early childhood teachers of junior colleges. It also hopes to find effective ways to manage the department of education.

• Korean Journal of Poultry Science
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• v.48 no.3
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• pp.111-122
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• 2021

Recently, interleukin 34 (IL-34) was identified as the second functional ligand for macrophage colony-stimulating factor receptor (M-CSFR). IL-34 functions similarly to M-CSF through its binding to the M-CSFR. There is still insufficient information on IL-34 in chickens, which has until now been reported only through predicted sequences and not through experimental research. Thus, to confirm its expression and to determine its potent biological activity, several chicken lines and cell lines were used. Cloning of recombinant chicken IL-34 and M-CSF genes was performed to investigate their modulatory effects on proinflammatory cytokine expression in vitro. The expression levels of IL-34, M-CSF, and M-CSFR genes were upregulated in broiler chickens with leg dysfunction (cause unknown). However, IL-34 was downregulated in most pathogen-stimulated tissues. M-CSFR expression was enhanced by recombinant IL-34 and M-CSF proteins in vitro. IFN-γ expression was enhanced by recombinant IL-34, but not by M-CSF. However, IL-12 expression was not regulated in any of the treated cells, and IL-1β was decreased in all tissues. These results indicate that IL-34 and M-CSF have roles in both the classical and alternative macrophage activation pathways. Collectively, our findings demonstrate the expression of IL-34 in chickens for pathogenic trials, both in vitro and in vivo. Our results suggest that the IL-34 protein plays a role in both pro- and anti-inflammatory functions in macrophages. Therefore, further research is needed to determine the cytokines or chemokines that can be induced by IL-34 and to further elucidate the functions of IL-34 in the inflammatory pathway.

• Journal of Korea Entertainment Industry Association
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• v.13 no.7
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• pp.99-118
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• 2019

This study confirmed the followings based on analysis of language messages in 718 advertisement in , AMOREPACIFIC's beauty magazine, published from 1958 to 2018 by product categories, era, in terms of purchase information, persuasive expression, word type. First, the number of pieces among 1980s to 1990s advertisement were the largest and, in terms of product categories, there were the greatest number of pieces in skincare, makeup and mens products. Second, headline and bodycopy had a different aspect in persuasive expression. "focused on image-making" was mainly used for head lines. Specifically, "situational image" was generally dominant. While the "user image" was higher before 1990's, "brand image" was as recent times. "Informal" was mostly applied for bodycopies, especially, "general information" and "differentiated information" was used the most. It is important to know what kind of information the brand established in each brand should be embodied rather than simply dividing the appeal method into "rational appeal" and "emotional appeal."Third, persuasive expression has different aspects in headlines and body copies. "focused on image-making" was mainly used as headlines. Specifically, "situational image" is dominant. Also, "user image" was high before 1990s but "brand image" got higher in recent times. "Informal" was mostly used as body copies, especially "general information" and "differentiated information" were the most frequently selected. Therefore, it is important to apprehend which information to specify established images by brands, rather than to divide "rational appeals" and "emotional appeals". Lastly, categorizing word type into brand names and headlines, foreign language was the most dominant in brand names and Chinese characters in headline. Remarkably, brand names in native language temporarily high in 70's and 80's, which could be interpreted to be resulted from the government policy promoting native language brands in those times. In addition, foreign language was frequently used in cosmetics and Chinese characters in men's product. It could be explained that colors or seasons in cosmetic products were expressed in foreign language in most case. On the other hand, the inclination of men's product consumers, where they pursue prestige or confidence in Chinese character, was actively reflected to language messages.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.16 no.4
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• pp.654-660
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• 2012

The goal of this paper is to calculate flood elevation by applying temporal distribution of rainfall through HEC-RAS(Hydrologic Engineering Center's River Analysis System) and to automatically create areas of flooding by a user-defined spatial model based on GIS using calculated values of flood elevation and detailed data of topography. Accuracy of topographic data is the most important factor because of deeply changing analysis results of flooding areas of a river. Therefore, this paper suggests a method of attributive and spatial data construction based on the GIS using UIS(Urban Information System, river-related reports, and hydrologic information. Also, we implement an expression system to provide analysis results extracted from the proposed model.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.11 no.1
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• pp.14-19
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• 2007

As XML is used by standard format for information expression and information exchange in various field, Many Company began to use SVG by user interface or information expression tool of embedded system such as mobile phone based wireless internet. Because SVG has many additional information to keep structure of SVG document exception real data, there is a problem that transfer time of SVG file is so cost for quantity of transmitted data actually. To solve this problem, many researches using compression conception have been conducted for applying to an embedded system. This paper proposes SMETA that can use existing researches using compression concept at once. SMETA divides SVG file to each part that can allocate meaning, and gives semantic metadata to each part without alteration of SVG structure. SMETA can reduce site of transmitted SVG file actually by transmitting specification part of SVG file that metadata does not agree or has unlisted part in an embedded system between an embedded system and server, before transmit whole SVG file. By size of transmitting SVG file is decreasing, transfer time can be shortened accordingly.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2016.10a
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• pp.904-906
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• 2016

In this paper, advertising and public relations services to provide kincet to implement a control system using face recognition and media. Traditionally, this unilateral system for delivering advertisements to consumers, is currently directed interactive ads. In other words, interactive advertising service that offers bi-directional communication. In the proposed system, the kincet using face recognition, to recognize faces with eyes, mouth, jaws and eyebrows. Presently used kinect version is the face of instability in the recognition and accurately is difficult to separate the three parts of the jaw, eyes, eyebrows only leverage. the classification has an easy immoyangProvide control services by separating the media, Hwanam-myeon, laughing. Also, consumers understand the expression of the control of the media and using picture for advertising to consumers through the transfer on both had indelibly imprinted by advertising.Effective are expected to appear.

• Smart Media Journal
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• v.10 no.4
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• pp.63-70
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• 2021

Public Signs guiding drivers on the road should help make decisions within a short period of time, so appropriate information should be effectively expressed at the point. Currently, the road marking rules revised by the law show a significant change in the type of information, expression method, and weight of expression compared to existing road signs to be based on road name addresses. This study analyzed whether the revised road signs changed based on the road name were effective in perspective of perception-cognition-understanding-projection and examined whether they provided appropriate information for the actual driving environment. Based on the analyzed information, an experiment was conducted to present an improved design, evaluate the degree of visibility compared to existing road name-oriented signs, and a qualitative investigation was conducted on the suitability of the sign contents. Through this, it is expected that more suitable driving information and measures to increase intuitive visibility can be reviewed.

• Journal of Life Science
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• v.19 no.6
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• pp.809-817
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• 2009

The phenotypes associated with a gene function are often the best clue to its role in the plant. Transgenic plants ectopically expressing or suppressing a gene can provide useful information related to the gene function. In this study, we constructed three vectors - pFGL571, pFGL846 and pFGL847 - for the Agrobacterium-mediated ectopic expression of plant genes using pPZP211 and modified CaMV 35S, UBQ3 or UBQ10 promoters. The three vectors have several merits such as small size, high copy in bacteria, enough restriction enzyme sites in multi cloning sites and nucleotide sequence information. Analysis of transgenic plants containing GUS or sGFP reporter genes under the control of modified CaMV 35S, UBQ3 or UBQI0 promoter revealed that all of the three promoters showed high activities during most developmental stages after germination and in floral organs. Furthermore, we generated a RNAi module vector, pFGL727, to suppress plant gene expressions and confirmed that pFGL727 is useful for the suppression of a gene expression using rice transgenic plants. Taken together, our new vectors would be very useful for the ectopic expression or the suppression of plant genes.