• 한국환경과학회지
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• 제33권1호
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• pp.97-102
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• 2024

We introduce a method for preserving yellow mealworm (Tenebrio molitor Linnaeus) larvae for an extended period and show that a high percentage of larvae can survive in good health under low-temperature storage conditions combined with specific diapause termination conditions. Our study revealed that we can regulate important development stages such as pupation, emergence, and larval duration by adjusting these termination conditions. When storing larvae aged 6-8 weeks for 140 days, the storage temperature can be varied based on our goals, giving us control over yellow mealworm production to meet specific demands. To produce adult beetles, storing larvae at 15 ℃ with wheat bran and ending diapause at 30 ℃ resulted in 90% pupation rate, with 60% becoming adults in 21 days. If our aim is larvae production, storing them at 10-12 ℃ with wheat bran and ending diapause at 25-30 ℃ allows the larvae to reach a suitable weight for processing. This straightforward approach ensures long-term storage of yellow mealworm larvae and provides a practical way to control their development, allowing efficient mass production tailored to market demands.

• 생태와환경
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• 제53권4호
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• pp.374-379
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• 2020

흰점박이꽃무지(Protaetia brevitarsis) 유충은 동아시아에서 식용 및 약용으로 널리 사용되고 있다. 흰점박이꽃무지 수요 증가로 인해 유충의 대량 사육 방법이 필요하지만 좁은 공간에서 대량으로 유충을 사육할 경우 곤충 병원성 질병 감염에 취약해진다. Metarhizium anisopliae는 흰점박이꽃무지 유충에 치명적인 곤충 병원성 곰팡이 중 하나이다. 이를 저해하기 위해 항진균 성분이 함유된 옻나무(Rhus verniciflua Stokes) 추출액을 첨가하였고, 연구 결과 1% 옻나무 추출액을 첨가한 경우 녹강균에 의한 유충의 치사율이 유의미하게 낮았다. 또한 일반톱밥을 급여한 대조구에 비해 옻나무 추출액 처리구에서 유충의 무게가 더 무거웠고 유충 기간이 짧아지는 것을 확인하였다. 따라서 이 연구를 통해 곤충 병원성 곰팡이에 대한 옻나무 추출액의 항진균 효과를 확인할 수 있었으며 흰점박이꽃무지 유충 사육 시 옻나무 추출액을 첨가하여 급여한다면 병원성 곰팡이에 의한 유충의 치사율을 낮추고 증체량을 높이는 데 효과가 있을 것이다.

• International Journal of Industrial Entomology and Biomaterials
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• 제45권2호
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• pp.115-125
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• 2022

In this study, we investigated the developmental characteristics and genetic diversity of seven populations of two-spotted crickets (Gryllus bimaculatus De Geer, 1773 (Orthoptera: Gryllidae)) raised in South Korea. Regarding the developmental characteristics of the species, we observed no statistically significant difference in the weight of the nymphs in the six populations we tested. After molting, although weight differences were observed between the populations in each stage of the developmental period, the average weight for each developmental stage was constant. We also analyzed mitochondrial COI gene sequences (DNA barcoding region) of the reared crickets collected from five insect farms and two national insect rearing facilities and the resultant sequences were analyzed together with the 12 sequences from foreign countries specimens obtained from public data. We detected six haplotypes from 111 specimens, indicating a low intraspecific genetic distance (~1.8%). The most dominant haplotype was overwhelmingly haplotype 1, which was found in all South Korean specimens and four specimens from China, Indonesia, and Germany. These findings indicate that the low genetic diversity of South Korean specimens can be explained by the fact that the G. bimaculatus population imported for feed from Japan in the early 2000s became a maternal group that spread throughout cricket farms in South Korea. In order to breed healthy cricket strains, it is necessary to increase genetic diversity by importing them from other countries through appropriate quarantine procedures.

• 농촌지도와개발
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• 제28권1호
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• pp.41-52
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• 2021

It is important to understand the factors that affect the business performance of insect farm for continuous insect farm management. The purpose of this study is to investigate factors influencing the business performance of insect farm. For this study, 1,577 questionnaires were collected through a telephone survey targeting insect farm owner. As a result of analysis using linear multiple regression analysis, the factors affecting total sales were gender, age, business experience, number of workers, and national and local government support projects. The factors affecting the net profit rate were age, business experience, number of workers, national and local government support projects, and education. When the gender of the business operator is male, it only affected the increase in total sales, and it was found that both the total sales amount and the net profit margin increased with the younger the business operator's age.

• International Journal of Industrial Entomology and Biomaterials
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• 제1권2호
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• pp.91-93
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• 2000

Insects have many excellent features and functions in their small bodies, such as hexapod walking, flapping flight, vision systems, sensory hairs, etc, and those characteristics can be thought as good models for many types of robots. Insects also will be good models far micro-machines because of its size. Insect behavior consists of simple reflex acts and programmed behavior, Some robots were made in order to clarify the emergent mechanism of insect behavior, Through some experiments it would be found that even if insect behavior consists of some simple action patterns, it looks intelligent through interactions its sensors and actuators with its complex environment. In the near futures small robots inspired by insects will be used in many fields of our life. I hope that insect-model based robots will play an active part in many fields and that they will make us happy.

• International Journal of Industrial Entomology and Biomaterials
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• 제33권1호
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• pp.15-23
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• 2016

One major constraint in the development and implementation of a successful and cost-effective area-wide integrated pest management (AW-IPM) programme with a SIT component for Bactrocera oleae (Diptera: Tephritidae) is the ability to produce a large number of high quality mass-reared individuals. The aim of this study was to develop a more efficient and practical egg collection system in an attempt to improve the mass-rearing of this species. The following basic parameters were examined: egg production per female, egg hatch, pupal recovery, pupal weight, adult emergence and percentage of fliers. Three different strains (Israel wild-type, France wild-type, and Greece laboratory) were tested and each strain was evaluated for six generations. Female flies of the Israel strain produced significantly more eggs per female than the other two strains, but egg hatch was significantly lower. Egg hatch of the France wild type and the Greece laboratory strain was similar. For all other parameters, there was no significant difference between strains; however, there was a significant generational effect for all parameters observed. As a result of this study, a protocol was developed for the mass-rearing of this species that included the use of large adult holding cages that could house up to 96,000 flies per cage. The newly developed method of egg collection using a flat wax panel as one of the sides of an adult holding cage proved to be cost-effective, efficient, making colony growth easier for industrial mass-rearing.

• International Journal of Industrial Entomology and Biomaterials
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• 제45권2호
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• pp.84-92
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• 2022

White-Jade silkworm (previously also known as Baegokjam) variety is the most popular silkworm variety that produces white cocoons. In 2021, the market share of White-Jade variety in Korea is very high, accounting for 88% of the silkworm production. Daebaekjam, Dodamsilkworm, Kumkangjam, and Kumokjam varieties, which have recently been established, make white cocoons like White-Jade. In this study, we found that 5 types of HongJams produced from 5 varieties of silkworms producing white cocoons did not show any severe difference in proximate analysis. The amounts of crude proteins, the most abundant nutrient, were between 71.05 ~ 73.38%, and those of crude lipids were 13.89 ~ 14.69% in 5 types of HongJams. In addition, there was no difference between White-Jade HongJam (WJ) and Daebaekjam HongJam in amino acid compositions. The amount of unsaturated fatty acid was significantly higher in WJ than in the other four types of HongJams, but the omega-6 fatty acids/omega-3 fatty acids ratio was higher in the four types of HongJams. Most of the minerals were higher in four types of HongJams than in WJ, and three heavy metal were not detected in all 5 types of HongJams. Phytochemicals were also most abundant in WJ, but the difference in the amounts were not severe. And pepsin digestibility was the highest for Kumokjam HongJam and the lowest for Dodam-silkworm HongJam, but the difference was not severe. The nutritional component analysis results of this study suggested that four new varieties can be used for producing HongJams, and Daebaekjam can replace White-Jade the most as the protein source.

• International Journal of Industrial Entomology and Biomaterials
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• 제45권2호
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• pp.99-107
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• 2022

Silkworm pupal extracts (SPE) were prepared in different solvents (water, 30%, 50%, 70%, and 100% ethanol) and their anti-inflammatory effects were evaluated in the RAW264.7 cell line. The SPE composition was initially evaluated by determining the protein content and performing Fourier transform infrared (FTIR) analysis. The protein content of the different SPE ranged from 6.75-130.93 mg/g of extract. FTIR analysis exhibited distinguishable absorption peaks among the extracts and indicated the presence of lipids, proteins, carbohydrates, and nucleic acid moieties. The levels of released nitric oxide (NO) and interleukin-6 (IL-6) expression in lipopolysaccharide (LPS)-induced RAW264.7 cells were only attenuated by 100% ethanolic SPE to 19.44% and 16.77%, respectively. The other solvent extracts were ineffective. Hence, further studies were conducted with 100% ethanolic SPE from three distinct stages of male and female silkworm pupae belonging to four silkworm varieties (Baegokjam; B, GoldenSilk; G, Juhwangjam; J, and YeonNokjam; Y). The best reduction in NO release and interleukin-1β (IL-1β) expression levels was achieved by the SPE of early female pupae belonging to the Baegokjam variety (32.72%) and those of early female pupae belonging to the Baegokjam and GoldenSilk (59.93%) varieties, respectively. The best reduction in IL-6 expression by 49.70% was achieved by SPE from female pupae of the mid-pupal stage belonging to the Baegokjam variety.

• 생명과학회지
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• 제30권12호
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• pp.1109-1117
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• 2020

아메리카왕거저리 유충 인공사료의 영양학적 우수성과 안전성을 검증하고자 인공사료와 밀기울로 사육한 아메리카왕거저리 유충의 영양성분과 유해물질을 비교분석하였다. 건조중량 기준 조단백질 함량은 인공사료로 사육한 아메리카왕거저리 유충(AD)에서 62.4%로 밀기울로 사육한 아메리카왕거저리 유충(WB) 45.2%보다 1.4배 더 많았다. 조지방은 AD에서 20.5%로 WB 46.3%보다 2.3배 더 적었다. 필수아미노산 중 로이신 함량이 가장 많았으며, AD에서 4.2%로 WB (3.0%)보다 1.4배 더 많았다. 비필수아미노산 또한 글루탐산 함량이 AD (7.0%)에서 WB (5.3%)보다 1.3배 더 많았다. 불포화지방산 중 올레산 함량은 WB (37.0%)에서 AD (26.7%)보다 1.4배 더 많은 것으로 나타났다. 다량무기질 중 가장 많은 함량을 나타낸 칼륨은 AD (975.9 mg/100 g)에서 WB (872.9 mg/100 g)보다 1.1배 더 많았다. 미량무기질 중 아연은 WB (6.5 mg/100 g)와 AD (6.3 mg/100 g)에서 비슷한 함량을 나타냈다. WB와 AD의 유해물질 분석 결과, 중금속 중 납, 카드뮴은 식용곤충의 중금속 기준에 적합한 수준이었고, 병원성 미생물인 대장균, 대장균군, 살모넬라균은 WB와 AD 모두에서 불검출되었다. 위의 영양성분 및 유해물질 분석결과로 볼 때, 인공사료로 사육한 아메리카왕거저리 유충은 다양한 영양성분을 함유하고 있으며, 안전성이 입증되었으므로 식용 및 사료용으로 활용하기에 유용할 것으로 판단된다.

• International Journal of Industrial Entomology and Biomaterials
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• 제3권1호
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• pp.97-99
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• 2001

Seven vegetables were planted in green houses to compare their yields and damages by insect pests based on three treatments, green music (GM), ultra sonic (US) and control. The vegetable yields of GM treatment were increased by an average of 17.81% than that of control for two years. There was a remarkable statistic difference in the yield between GM and respective controls such as radish, young radish, cucumber, spinach and Brassica oleracea var. acephala. There was no notable yield difference between US and controls for the semen vegetables within two years. The population densities of the insect pests in GM and US treatments were lower than controls. Only a few insect pests of some vegetables in GM and US treatments injured more seriously than in control.