International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Evaluation of the anti-inflammatory effects of silkworm (Bombyx mori L.) pupal extracts against lipopolysaccharide-induced inflammation in the murine macrophage cell line RAW264.7

  • Kamidi, Rahul (Industrial Insect and Sericulture Division, National Institute of Agricultural Sciences, RDA) ;
  • HaeYong, Kweon (Industrial Insect and Sericulture Division, National Institute of Agricultural Sciences, RDA) ;
  • Hun-bok, Kim (Industrial Insect and Sericulture Division, National Institute of Agricultural Sciences, RDA) ;
  • Ji Hae, Lee (Industrial Insect and Sericulture Division, National Institute of Agricultural Sciences, RDA)
  • Received : 2022.10.25
  • Accepted : 2022.12.12
  • Published : 2022.12.30
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Abstract

Silkworm pupal extracts (SPE) were prepared in different solvents (water, 30%, 50%, 70%, and 100% ethanol) and their anti-inflammatory effects were evaluated in the RAW264.7 cell line. The SPE composition was initially evaluated by determining the protein content and performing Fourier transform infrared (FTIR) analysis. The protein content of the different SPE ranged from 6.75-130.93 mg/g of extract. FTIR analysis exhibited distinguishable absorption peaks among the extracts and indicated the presence of lipids, proteins, carbohydrates, and nucleic acid moieties. The levels of released nitric oxide (NO) and interleukin-6 (IL-6) expression in lipopolysaccharide (LPS)-induced RAW264.7 cells were only attenuated by 100% ethanolic SPE to 19.44% and 16.77%, respectively. The other solvent extracts were ineffective. Hence, further studies were conducted with 100% ethanolic SPE from three distinct stages of male and female silkworm pupae belonging to four silkworm varieties (Baegokjam; B, GoldenSilk; G, Juhwangjam; J, and YeonNokjam; Y). The best reduction in NO release and interleukin-1β (IL-1β) expression levels was achieved by the SPE of early female pupae belonging to the Baegokjam variety (32.72%) and those of early female pupae belonging to the Baegokjam and GoldenSilk (59.93%) varieties, respectively. The best reduction in IL-6 expression by 49.70% was achieved by SPE from female pupae of the mid-pupal stage belonging to the Baegokjam variety.

Keywords

Acknowledgement

This research activity was supported by the 'Research Program for Agriculture Science and Technology Development' (Grant No. PJ01502202), National Institute of Agricultural Sciences, Rural Development Administration, Republic of Korea.

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