• Journal of the East Asian Society of Dietary Life
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• v.9 no.2
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• pp.200-206
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• 1999

This study was carried out to get a industrial information about a possibility of IgY antibody production, antimicrobial activity and Properties of IgY antibody in egg yolk. After the initial immunization the anti-Salmonella typhimurium IgY antibody level gradually were decreased from firth week to tenth week. On the other hand, the antibody level in the serum were increased from the first week, reaching its peak in the sixth week. Molecular weights of IgY were estimated approximately 72-75KD in a heavy chain and 30-40KD in a light chain by electrophoresis.

• Analytical Science and Technology
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• v.17 no.1
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• pp.37-44
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• 2004

An alcohol oxidase from Hansenula polymorpha was strongly induced when cells were grown with 0.5% methanol supplementation as the carbon source. The induced Hansenula polymorpha alcohol oxidase was purified to electrophoretic homogeneity by using DEAE-Sephacel and Mono Q column chromatographys. The enzyme oxidized mainly primary aliphatic alcohols and exhibited high substrate specificity towards ethanol and methanol. The activity of the enzyme optimally proceeded at pH 8.5 and $50^{\circ}C$. The midpoint of the temperature-stability curve for the enzyme was approximately $52^{\circ}C$ and the enzyme was not completely inactivated even at $65^{\circ}C$ temperature. The enzyme showed resistance toward detergents and highly stable over 7 weeks of storage condition. This Hansenula polymorpha alcohol oxidase may be useful for the enzymatic determination of alcohol and for the industrial production of alcohols and aldehydes.

• Korean Journal of Food Science and Technology
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• v.32 no.5
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• pp.985-990
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• 2000

${\zeta}-Carotene$ was subjected to ozonolysis in ice-cold dichloromethane. The ozonolysis products were fractionated with a silica column and the carbonyl fraction was analyzed by ODS-HPLC with a photodiode array detector. ${\zeta}-Carotene$ was solubilized in toluene, and then oxidized by incubating at $37^{\circ}C$, 72 hr under atmospheric oxygen. Carbonyl compound and acidic compound were produced. In comparison with autoxidation and ozonolysis, each compound showed the same retention time and UV-vis spectra were identical to the reference cleavage products prepared by ozonolysis of ${\zeta}-carotene$. Absorption spectrum of acidic compound was similar to that of standard 4,5-didehydrogeranyl geranyl acid which is known to possess biological activity. Thus, eccentric cleavage of ${\zeta}-carotene$ was confirmed to occur in vitro under oxidation condition.

• Archives of Pharmacal Research
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• v.22 no.2
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• pp.219-224
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• 1999

In our search for the anticonvulsant consitutent of Gastrodia elata repeated column chromatographies guided by activity assay led to isolation of an active compound, which was identified as gastrodin on the basis of spectral data. Brain succinic semialdehyde dehydrogenase (SSADH) was inactivated by preincubation with gastrodin in a time-dependent manner and the reaction was monitored by absorption and fluorescene spectroscopic methods. The inactivation followed pseudo-first-order kinetics with the second-rate order constant of $1.2{\times}10^{3} M^{-1} min^{-1}$. The time course of the reaction was significantly affected by the coenzyme NAD^{+}$, which affected complete protection against the loss of the catalytic activity, whereas substrate succinic semialdehyde failed to prevent the inactivation of the enzyme. It is postulated that the gastrodin is able to elevate the neurotransmitter GABA levels in central nervous system by inhibitory action on one of the GABA degradative enzymes, SSADH.

• Food Engineering Progress
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• v.15 no.3
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• pp.276-281
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• 2011

The optimum condition for the extraction and purification processes of phytic acid from defatted rice bran was examined. The phytic acid was efficiently extracted when the defatted rice bran was treated with 10 volumes of 0.5% HCl for 1 hr. For the neutralization of acid-treated extract, 0.5% NaOH was the most acceptable. To purify phytic acid, Diaion HP20 resin was used to remove impurities from the extract. The flow-through was then loaded onto ion exchange columns packed with various resins and among them, Amberlite IRA-416 resin showed highest recovery yield. When the phytic acid was absorbed onto Amberlite IRA-416 resin and then eluted with 0.5% NaOH, 89% of applied phytic acid was eluted. Most proteins were removed from the purified phytic acid and total protein content of the phytic acid was 0.14%(w/w).

• Journal of Ginseng Research
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• v.43 no.1
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• pp.105-115
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• 2019

Background: Ginsenosides with less sugar moieties may exhibit the better adsorptive capacity and more pharmacological activities. Methods: An efficient method for the separation of four minor saponins, including gypenoside XVII, notoginsenoside Fe, ginsenoside Rd2, and notoginsenoside Fd, from Panax notoginseng leaves (PNL) was established using biotransformation, macroporous resins, and subsequent preparative high-performance liquid chromatography. Results: The dried PNL powder was immersed in the distilled water at $50^{\circ}C$ for 30 min for converting the major saponins, ginsenosides Rb1, Rc, Rb2, and Rb3, to minor saponins, gypenoside XVII, notoginsenoside Fe, ginsenoside Rd2, and notoginsenoside Fd, respectively, by the enzymes present in PNL. The adsorption characteristics of these minor saponins on five types of macroporous resins, D-101, DA-201, DM-301, X-5, and S-8, were evaluated and compared. Among them, D-101 was selected due to the best adsorption and desorption properties. Under the optimized conditions, the fraction containing the four target saponins was separated by D-101 resin. Subsequently, the target minor saponins were individually separated and purified by preparative high-performance liquid chromatography with a reversed-phase column. Conclusion: Our study provides a simple and efficient method for the preparation of these four minor saponins from PNL, which will be potential for industrial applications.

• Journal of IKEEE
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• v.23 no.3
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• pp.954-961
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• 2019

This paper suggests an advanced 8T SRAM which can operate properly in subthreshold voltage regime. The memory cell consists of symmetric 8 transistors, in which the latch storing data is controlled by a column-wise assistline. During the read, the data storage nodes are temporarily decoupled from the read path, thus eliminating the read disturbance. Additionally, the cell keeps the noise-vulnerable 'low' node close to the ground, thereby improving the dummy-read stability. In the write, the boosted wordline facilitates to change the contents of the memory bit. At 0.4 V supply, the advanced 8T cell achieves 65% higher dummy-read stability and 3.7 times better write-ability compared to the commercialized 8T cell. The proposed cell and circuit techniques have been verified in a 16-kbit SRAM array designed with an industrial 180-nm low-power CMOS process.

• Journal of the Architectural Institute of Korea Structure & Construction
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• v.33 no.12
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• pp.37-44
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• 2017

Due to structural characteristics, construction costs and duration of a modular system would be saved by minimizing the schedule on the job site. As such, it is crucial to develop a connection that can guarantee stiffness while allowing for simple assembling. Particularly, the mid- to high-rise construction of the modular system necessitates the securing of the structural stability and seismic performance of multi-unit frames and connections, and thus, the stiffness of unit-assembled structures needs to be re-evaluated and designed. However, evaluating a frame consisting of slender members and reinforcing materials is a complicated process. Therefore, the present study aims to examine the structural characteristics of a modular unit connection based a method for reinforcing connection brackets and hinges while minimizing the loss of the cross section. Toward this end, the study modeled the beam-to-column connection of a modular system with the proposed connection, and produced a specimen which was used to perform a cycling loading test. The study compared the initial stiffness, the attributes of the hysteretic behavior, and the maximum flexural moment, and observed whether the model acquired the seismic performance, compared to the flexural strength of the steel moment frame connection that is required by the Korean Building Code. The test results showed that the proposed connection produced a similar initial stiffness value to that of the theoretical equation, and its maximum strength exceeded the theoretical strength. Furthermore, the model with a larger ceiling bracket showed higher seismic performance, which was further increased by the reinforcement of the plate.

• Journal of the Architectural Institute of Korea Structure & Construction
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• v.34 no.3
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• pp.19-28
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• 2018

Key factors that ensure competitiveness of modular unit include consistent high quality and connection condition that ensures high structural performance while minimizing the overall scale of the on-site process. However, it is difficult to evaluate the structural performance of the connection of modular unit, and its structural analysis and design method can be different depending on the connection to its development, which affects the seismic performance of its final design. In particular, securing the seismic performance is the key to designing modular systems of mid-to-high-rise structure. In this paper, therefore, the seismic performance of the modular system with bracket-typed fully restrained moment connections according to stiffness and the shapes of various connection members was evaluated through experimental and analytical methods. To verify the seismic performance, a cyclic loading test of the connection joint of the proposed modular system was conducted. As a result of this study, theoretical values and experimental results were compared with the initial stiffness, hysteresis behavior and maximum bending moment of the modular system. Also, the connection joint was modeled, using the commercial program ANSYS, which was then followed by finite element analysis of the system. According to the results of the experiment, the maximum resisting force of the proposed connection exceeded the theoretical parameters, which indicated that a rigid joint structural performance could be secured. These results almost satisfied the criteria for connection bending strength of special moment frame listed on KBC2016.

• Journal of the Architectural Institute of Korea Structure & Construction
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• v.34 no.4
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• pp.25-33
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• 2018

In regard to modular systems, new methods, as well as middle and high-story unit design ideas, are currently being studied. These studies need to focus on the enhanced stiffness and seismic performance of these connections, and see that the development of fully restrained moment connections can improve the seismic performance. For this reason, this study evaluates the performance of the connections of the ceiling bracket-typed modular system through repeated loading tests and analyses. In order to compare them with these modular units, new unit specimens with the bracket connection being different from that of the traditional modular unit specimens were designed, and the results of repeated loading tests were analyzed. In the traditional units, the structural performances of both welding connection and bolt connection were evaluated. In regard to the testing results, the initial stiffness of the hysteresis curve was compared with the theoretical initial stiffness, and the features of all specimens were also analyzed with regard to the maximum moment. In addition, the test results were examined with regard to the connection flexural strength of the steel special moment frame specified under the construction criteria KBC2016. The connections, which were proposed in the test results, were found to be fully restrained moment connections for designing strong column-weak beams and meeting the requirements of seismic performance of special moment frames.