• BMB Reports
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• 제38권4호
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• pp.440-446
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• 2005

A cDNA that was rapidly induced upon abscisic acid, cold, drought, mechanical wounding and to a lesser extent, by high salinity treatment, was isolated from Arabidopsis seedlings. It was classified as DREB subfamily member based on multiple sequence alignment and phylogenetic characterization. Since it encoded a protein with a typical ERF/AP2 DNA-binding domain and was closely related to the TINY gene, we named it TINY2. Gel retardation assay revealed that TINY2 was able to form a specific complex with the previously characterized DRE element while showed only residual affinity to the GCC box. When fused to the GAL4 DNA-binding domain, either full-length or its C-terminus functioned effectively as a trans-activator in the yeast one-hybrid assay while its N-terminus was completely inactive. Our data indicate that TINY2 could be a new member of the AP2/EREBP transcription factor family involved in activation of down-stream genes in response to environmental stress.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2006년도 춘계학술대회논문집
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• pp.122-125
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• 2006

The Substructure Synthesis means the technology to predict the dynamic properties of an assembly from the properties of its components, or to predict the effect of a modification on a structure. The FRF Based Substructuring method is a kind of the Substructure Synthesis and very useful to predict the efficiency of the product in the early stage of development. Especially, the Hybrid FBS method is very useful to predict the vehicle NVH characteristics after modifying some components of the vehicle. Target components can be established on the basis of test models and FE models of the prototype constructed in the early stage of development. In this study, the Hybrid FBS method was applied to vehicle subframe and car-body in order to reduce vehicle interior noise induced by engine exciting force.

• Journal of Microbiology
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• 제34권1호
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• pp.90-94
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• 1996

The carboxymethyl cellulase (CMCase) was purified from the induced culture filtrate of hybrid TAPW15703 between Aspergillus niger and penicillium verruculosum made by nuclear transfer. The enzyme was purified 80 fold with an overall yield 17% from the culture medium by ammonium sulfate fractionation, Sephadex G-75 gel permeation chromatography, and DEAE-ion exchange column chromatography. The molecular weight of the CMCase has estimated to be 32,000 daltons on SDS-polyacrylamide gel electrophoresis and Sephadex G-150 gel permeation chromatography. The purified enzyme functions optimally at pH 4.0 and 4$0^{\circ}C$ The Km value for carbosymethyl cellulose was 68 mM. The enzyme activity was increased by the presence of $Mg^{2+}$and Mn$^{2+}$.

• 한국양식학회지
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• 제10권1호
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• pp.49-54
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• 1997

무지개송어, 산천어, 무지개송어와 산천어간의 잡종 3배체를 대상으로 성비조사와 형태학적 계측형질을 측정하였다. 무지개송어, 산천어의 암.수 성비는 공히 유사한 비율로 나타난 반면, 잡종과 잡종 3배체에서는 수컷이 암컷에 비해 높게 나타났다(p<0.01). 잡종 3배체는 두고/두장, 등지느러미 기부장/체장 및 가슴지느러미 길이/체장의 계측형질에서는 친어로 사용된 무지개송어와 산천어의 중간을 나타낸 반면 상악장/두장의 계측형질에서는 무지개송어 쪽을 다소 닮게 나타났다. 이러한 결과들은 잡종 3배체에서 3배체화에 따른 사용된 친어들에 대비된 성비 그리고 계측형질에서의 특징을 나타내었다.

• 대한의생명과학회지
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• 제9권2호
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• pp.105-110
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• 2003

Ankyrins are a ubiquitously expressed family of intracellular adaptor proteins involved in targeting diverse proteins to specialized membrane domains in both the plasma membrane and the endoplasmic reticulum. Canonical ankyrins are 190-220 kDa proteins expressed in most tissues and cell types and comprise a membrane-binding domain (MBD) of 24 ANK repeats, a spectrin-binding domain, a death domain and a C-terminal domain. Rescue studies with ankyrin-B/G chimeras have identified the C-terminal domain of ankyrin-B as the defining domain in specifying ankyrin-B activity, but the function of C-terminal domain of ankyrin-B is, however, not known. We report here that the C-terminal domain of ankyrin-B is capable of interacting with the C-terminal Region of Hsp40. The Hsps are induced not only by heat shock but also by various other environmental stresses. Hsps are also expressed constitutively at normal growth temperatures and have basic and indispensable functions in the life cycle of proteins as molecular chaperones, as well as playing a role in protecting cells from the deleterious stresses. The binding sites required in the interaction between C-terminal domain of ankyrin-B and C-terminal region of Hsp40 were characterized using the yeast two-hybrid system and GST-pull down assay. The interaction between ankyrin-B and Hsp40 represents the first direct evidence of ankyrin's role as chaperones.

• Geomechanics and Engineering
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• 제27권5호
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• pp.511-525
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• 2021

In the design of underground excavation, the shear strength (SS) is a key characteristic. It describes the way the rock material resists the shear stress-induced deformations. In general, the measurement of the parameters related to rock shear strength is done through laboratory experiments, which are costly, damaging, and time-consuming. Add to this the difficulty of preparing core samples of acceptable quality, particularly in case of highly weathered and fractured rock. This study applies rock index test to the indirect measurement of the SS parameters of shale. For this aim, two efficient artificial intelligence methods, namely (1) adaptive neuro-fuzzy inference system (ANFIS) implemented by subtractive clustering method (SCM) and (2) support vector regression (SVR) optimized by Harmony Search (HS) algorithm, are proposed. Note that, it is the first work that predicts the SS parameters of shale through ANFIS-SCM and SVR-HS hybrid models. In modeling processes of ANFIS-SCM and SVR-HS, the results obtained from the rock index tests were set as inputs, while the SS parameters were set as outputs. By reviewing the obtained results, it was found that both ANFIS-SCM and SVR-HS models can provide acceptable predictions for interlocking and friction angle parameters, however, ANFIS-SCM showed a better generalization capability.

• Asian Journal of Atmospheric Environment
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• 제6권2호
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• pp.104-110
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• 2012

We studied the effects of elevated ozone ([$O_3$]) and $CO_2$ concentrations ([$CO_2$]) on the growth and photosynthesis of the hybrid larch $F_1(F_1)$ and on its parents (the Dahurian larch and Japanese larch). $F_1$ is a promising species for timber production in northeast Asia. Seedlings of the three species were grown in 16 open top chambers and were exposed to two levels of $O_3$ (<10 ppb and 60 ppb for 7 h per day) in combination with two levels of $CO_2$ (ambient and 600 ppm for daytime) over an entire growing season. Ozone reduced the growth as measured by height and diameter, and reduced the needle dry mass and net photosynthetic rate of $F_1$, but had almost no effect on the Dahurian larch or Japanese larch. There was a significant increase in whole-plant dry mass induced by elevated [$CO_2$] in $F_1$ but not in the other two species. Photosynthetic acclimation to elevated [$CO_2$] was observed in all species. The net photosynthetic rate measured at the growing [$CO_2$] (i.e. 380 ppm for ambient treatment and 600 ppm for elevated $CO_2$ treatment) was nevertheless greater in the seedlings of all species grown at elevated [$CO_2$]. The high [$CO_2$] partly compensated for the reduction of stem diameter growth of $F_1$ at high [$O_3$]; no similar trend was found in the other growth and photosynthetic parameters, or in the other species.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2011년도 정기총회 및 추계학술대회 논문집
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• pp.119-127
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• 2011

본 논문은 장거리 터널 내에서 운송체의 고속운행에 의해 발생한 유동을 해석하기 위한 격자 기법 개발에 관한 내용이다. 수십 km의 터널 시스템을 3차원으로 해석하기 위해서는 많은 수의 격자가 요구된다. 그러나 전체 터널 시스템을 3차원으로 계산한다는 것은 비효율적이다. 그리하여 본 논문에서 계산의 효율성을 위해 터널 내 유동장에 영향을 크게 주는 달리는 운송체 주위, 정거장과 환기구는 3차원으로 격자를 구성하였고 그 외의 직전 터널 구간은 1차원 격자로 구성하였다. 터널 내의 유동은 열차가 달릴 때 생성되는 열차 주위의 유동에 의해 결정된다. 그러므로 열차 주위에는 격자를 조밀하게 생성시켜 주었다. 그리고 이 격자는 열차가 이동할 때 함께 움직이도록 하여 격자의 질을 유지하게 하였다. 열차의 운행 속도는 가속, 등속, 감속의 3단계로 구분하였다. 이와 같은 장거리 터널 내 비정상 고속 운송체 유동을 해석하기 위해 상용 소프트웨어인 Ansys vr. 12.0의 UDFs(User-Defined Functions) 기능을 사용하였다.

• International Journal of Control, Automation, and Systems
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• 제5권6호
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• pp.643-651
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• 2007

The various blood pressure simulators have been proposed to evaluate and improve the performance of the automatic sphygmomanometer. These have some problems such as the deviation of the actual blood pressure waveform, limitation in the blood pressure condition of the simulator, or difficulty in displaying the blood flow. An improved simulator using disturbance observer is proposed to supplement the current problems of the blood pressure simulator. The proposed simulator has an artificial arm model capable of feeding appropriate fluids that can generate the blood pressure waveform to evaluate the automatic sphygmomanometer. A controller was designed and thereafter, simulation was performed to control the output signal with respect to the reference input in the fluid dynamic model using the proposed proportional control valve. To minimize the external fluctuation of pressure applied to the artificial arm, a disturbance observer was designed on the plant. A hybrid controller combined with a proportional controller and feed-forward controller was fabricated after applying a disturbance observer to the control plant. Comparison of the simulations between the conventional proportional controller and the proposed hybrid controller indicated that even though the former showed good control performance without disturbance, it was affected by the disturbance signal induced by the cuff. The latter exhibited an excellent performance under both situations.

• Smart Structures and Systems
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• 제20권4호
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• pp.483-498
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• 2017

Real-Time Hybrid Simulation (RTHS) is a powerful and cost-effective dynamic experimental technique. To implement a stable and accurate RTHS, time delay present in the experiment loop needs to be compensated. This delay is mostly introduced by servo-hydraulic actuator dynamics and can be reduced by applying appropriate compensators. Existing compensators have demonstrated effective performance in achieving good tracking performance. Most of them have been focused on their application in cases where the structure under investigation is subjected to inputs with relatively low frequency bandwidth such as earthquake excitations. To advance RTHS as an attractive technique for other engineering applications with broader excitation frequency, a discrete-time feedforward compensator is developed herein via various optimization techniques to enhance the performance of RTHS. The proposed compensator is unique as a discrete-time, model-based feedforward compensator. The feedforward control is chosen because it can substantially improve the reference tracking performance and speed when the plant dynamics is well-understood and modeled. The discrete-time formulation enables the use of inherently stable digital filters for compensator development, and avoids the error induced by continuous-time to discrete-time conversion during the compensator implementation in digital computer. This paper discusses the technical challenges in designing a discrete-time compensator, and proposes several optimal solutions to resolve these challenges. The effectiveness of compensators obtained via these optimal solutions is demonstrated through both numerical and experimental studies. Then, the proposed compensators have been successfully applied to RTHS tests. By comparing these results to results obtained using several existing feedforward compensators, the proposed compensator demonstrates superior performance in both time delay and Root-Mean-Square (RMS) error.