• 한국식품영양과학회지
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• 제33권7호
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• pp.1169-1174
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• 2004

본 연구에서는 감마선 및 전자선종이 ovalbumin(OVA)의 구조 변화에 미치는 영향을 비교 평가하였다. 표준항원 OVA을 3,5,7 및 10 kGy의 흡수선량으로 감마선 및 전자선 조사하였으며 그에 따른 구조적인 변화는 SDS-PAGE, GPC-HPLC 및 단클론 항체를 사용한 Ci-ELISA법으로 측정하였다. Native OVA분자는 감마선 및 전자선 조사에 의해 소편화 및 응집화 되었으며, 조사 선량이 증가할수록 OVA의 분자량이 감소하였다. 또한 OVA의 면역화학적 인 구조는 방사선 조사에 의해 항원-항체간의 결합능이 감소하는 것으로 나타났으나 감마선 및 전자선종에 따른 차이는 없었다. 이상의 결과들은 식품 알레르겐의 제거 및 면역원의 개발에 대한 전자선 조사의 기초자료로 이용될 수 있을 것으로 사료된다.

• 대한수의학회지
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• 제43권4호
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• pp.677-681
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• 2003

Currently, both the indirect fluorescent antibody test (IFAT) and enzyme linked immunosorbent assay (ELISA) have been used to detect Neospora caninum antibodies. Several factors such as the buffers, the conjugate, the pattern of fluorescence, and the cross reactivity with other apicornplexan protozoan, may result in poorly correlated data. The present study was undertaken to develop and evaluate the Neospora agglutination test (NAT) for the detection and quantification of IgG antibodies to N. caninum from various animal species. Compared to the ELISA method, the NAT with a cutoff value of 1:512 gave a high index of coincidence (kappa=0.807) and no cross reactivity to Toxoplasma gondii antiserum. Hence, this NAT method, which did not require a species-specific secondary antibody and expensive tools, would be easily available for the detection of antibodies to N. caninllm of various animal species.

• 대한바이러스학회지
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• 제26권1호
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• pp.59-65
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• 1996

For easy and rapid screening of hemorrhagic fever with renal syndrome (HFRS) without any laboratory equipment, dot blot enzyme immunoassay was developed and tried to detect anti-hantavirus antibodies. The nucleocapsid protein of Hantaan virus was isolated by affinity chromatography and used for making the dot strip. 28 of 29 Hantaan virus infected sera showed positive signals and 21 of 22 HFRS negative sera showed no positive signals. Anti-Seoul virus monoclonal antibody also exibited positive signal but the intensity of colorization was approximately 5 fold less than that of anti-Hantaan monoclonal antibody. The sensitivity of dot blot assay was equal or superior to indirect immunofluorescent assay (IFA) or ELISA test. Overall, the screening results with dot blot assay showed 92.2 % of concordance with IFA or ELISA test. This results suggests that dot blot assay could be applied a tool for easy and rapid screening of HFRS.

• BMB Reports
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• 제31권1호
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• pp.58-63
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• 1998

In this study, a mouse monoclonal antibody (mAb) against gp41(584-618), the immunodominant epitope protein, was generated. For this purpose, BALB/c mice were immunized with double branched multiple antigenic peptides derived from the HIV-1 gp41(584-618) sequence, and antibody-secreting hybridoma were produced by fusion of mice splenocytes with SP2/0 myeloma cells. One clone producing an antigen specific mAb, termed KI-41(isotype IgG1) was identified, whose specific reactivity against gp41(584-618) could be confirmed by ELISA and Western blot analysis. Epitope mapping revealed the recognition site of the mAb KI-41 to be located around the sequence RILAVERYLKDQQLLG, which comprises the N-terminal region within the immunized gp41(584-618) peptied. Since this mAb recognizes this specific epitope within the HIV-1 gp41 without any cross-reactivity to other immunodominant regions in the HIV-2 gp35, KI-41 will provide some alternative possibilities in further applications such as the development of indirect or competitive ELISA for specific antibody detection in HIV-1 infection or for other basic researches regarding the role and function of HIV-1 gp41.

• Restorative Dentistry and Endodontics
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• 제18권2호
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• pp.341-356
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• 1993

In the prevention of root surface caries, antimicrobial therapy for the control of subgingival and supragingival plaque is seriously considered as a long term suppression of pathogenic microflora. Recently, varnishes containing antimicrobial agents have been developed to control the supragingival microflora. The purpose of this study was to determine the antimicrobial effects of 20% chlorhexidine varnish and 2.6% silane fluoride varnish with sealant. In clinical experiments, 12 subjects were selected from the periodontally treated patient and divided into 3 groups. After a dental prophylaxis, the subjects were treated with single application of placebo varnish (group I), 20% chlorhexidine varnish (group II), and 2.6% silane fluoride varnish (group III). Root surface plaque samples were taken before (baseline) and one, two, four, and 8 weeks after the treatments. Microbiological examinations of root surface plaque were performed with culture study and indirect immunoflorescence (I.I.F.) study, and immunological examination of gingival crevicular fluid antibody titers was performed with ELISA study. The results were as follows: 1. Pathogenic microflora on the root surface including S. mutans, S. sanguis, S. mitis, A. naeslundii, A. viscosus were 24 - 37% on I.I.F. study. 2. S. mutans, S. sanguis, S. mitis, A. naeslundii, A. viscosus of the root surface plaque was significantly reduced from 1 week to 8 weeks after antimicrobial varnish treatment, but showed generally increasing tendency in control group. 3. Gingival crevicular fluid antibody titers were significantly reduced from 1 or 2 weeks to 4 weeks after antimicrobial varnish treatment.

• 생태와환경
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• 제49권4호
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• pp.393-399
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• 2016

국내 주요 상수원지 (영천호, 안계호, 가창호)에서 분리한 6종의 Microcystis (M. aeruginosa, M. ichthyoblabe, M. flos-aquae, M. novacekii, M. viridis 및 M. wesenbergii)를 대상으로 microcystin 생성유전자(mcy gene) 함유률 및 생성률을 조사하였다. 조사 결과에서 M. aeruginosa는 80%(55개 균주)가 mcy gene을 함유하고 있었으며, 그 다음으로 M. ichthyoblabe는 45% (10개 균주)의 함유율을 나타내었다. 그 외에 M. flos-aquae와 M. wesenbergii는 각각 11%(4개 균주)와 33% (1개 균주)가 microcystin 생성유전자를 포함하였다. 6종의 Microcystis에서 mcy gene의 함유율은 실제 microcystin 생성율과 거의 일치하였다. 특히, 가장 많은 균주에서 mcy gene이 확인된 M. aeruginosa는 대부분 균주들(75%)이 실제로 microcystin을 생성하는 것으로 조사되었다.

• Journal of Microbiology and Biotechnology
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• 제29권3호
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• pp.482-488
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• 2019

Porcine circovirus type 2 (PCV2) is the causative agent of postweaning multisystemic wasting syndrome (PMWS) in pigs. Replicase (Rep) proteins are considered essential for viral replication. Capsid (Cap) protein is the primary immunogenic protein that induces protective immunity. Little is known about comparison on the immunogenicity of PCV2 Rep and Cap fusion protein and Cap protein. In the present study, recombinant baculoviruses expressing the Rep-Cap fusion protein (Bac-Rep-Cap) and the Cap protein (Bac-Cap) of PCV2 were constructed and confirmed with western blot and indirect fluorescence assay. Immunogenicities of the two recombinant proteins were tested in mice. The titers of antibodies were determined with a PCV2-specific enzyme-linked immunosorbent assay (ELISA) and a serum neutralization assay. The $IFN-{\gamma}$ response of immunized mice was measured by ELISA. The mice immunized with the Bac-Rep-Cap and Bac-Cap successfully produced Cap-specific immunoreaction. The mice immunized with the Bac-Cap developed higher PCV2-specific neutralizing antibody titers than mice injected with the Bac-Rep-Cap. $IFN-{\gamma}$ in the Bac-Rep-Cap group was increased compared to those in the Bac-Cap group. Vaccination of mice with the Bac-Rep-Cap showed significantly decreased protective efficacy compared to the Bac-Cap. Our findings will indubitably not only lead to a better understanding of the immunogenicity of PCV2, but also improved vaccines.

• Journal of Veterinary Science
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• 제23권3호
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• pp.50.1-50.12
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• 2022

Background: There is an urgent need to find reliable and rapid bovine tuberculosis (bTB) diagnostics in response to the rising prevalence of bTB worldwide. Toll-like receptor 2 (TLR2) recognizes components of bTB and initiates antigen-presenting cells to mediate humoral immunity. Evaluating the affinity of antigens with TLR2 can form the basis of a new method for the diagnosis of bTB based on humoral immunity. Objectives: To develop a reliable and rapid strategy to improve diagnostic tools for bTB. Methods: In this study, we expressed and purified the sixteen bTB-specific recombinant proteins in Escherichia coli. The two antigenic proteins, MPT70 and MPT83, which were most valuable for serological diagnosis of bTB were screened. Molecular docking technology was used to analyze the affinity of MPT70, MPT83, dominant epitope peptide of MPT70 (M1), and dominant epitope peptide MPT83 (M2) with TLR2, combined with the detection results of enzyme-linked immunosorbent assay to evaluate the molecular docking effect. Results: The results showed that interaction surface Cα-atom root mean square deviation of proteins (M1, M2, MPT70, MPT83)-TLR2 protein are less than 2.5 A, showing a high affinity. It is verified by clinical serum samples that MPT70, MPT83, MPT70-MPT83 showed good diagnostic potential for the detection of anti-bTB IgG and M1, M2 can replace the whole protein as the detection antigen. Conclusions: Molecular docking to evaluate the affinity of bTB protein and TLR2 combined with ELISA provides new insights for the diagnosis of bTB.

• Pediatric Infection and Vaccine
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• 제12권2호
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• pp.108-113
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• 2005

목 적 : 최근 HHV-8는 면역력이 정상인 소아에서 원발성 감염에 의해 발진이 동반된 발열성 질환을 유발하는 것으로 알려지고 있다. 본 연구는 국내 정상 소아의 항 HHV-8 항체 양성률을 알아보기 위해 시행되었다. 방 법 : 항 HHV-8 항체의 양성률을 파악하기 위하여 인제의대 상계백병원을 방문한 환자 중에서, 최근 3주간 발열 증상이 없었던 건강한 소아 112명의 혈청을 대상으로 하였다. Lytic 바이러스 항원에 대한 IgG 항체 검사는 간접면역 형광검사법, HHV-8의 ORF에 대한 특이 항체 검사는 peptide mix ELISA 검사법을 사용하였다. 결 과 : 대상 환아는 총 112명이었으며 남아 64명, 여아 48명이었다. 1세 이하의 연령군은 17명이었으며, 2~5세 연령군 24명, 6~9세 연령군 24명, 10~15세 연령군은 47명이었다. HHV-8에 대한 혈청 항체 양성률은 간접 면역 형광검사법으로 3.5%(4/112), ELSIA로는 1.8%(2/112)였다. 결 론 : 국내 소아의 HHV-8 항체의 양성률은 비교적 낮았지만 앞으로 발진이 동반된 발열성 질환에서 HHV-8의 역할에 대한 추가적인 연구가 필요할 것으로 보인다.

• Parasites, Hosts and Diseases
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• 제35권1호
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• pp.55-62
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• 1997

톡소포자충(ToxopLasmn gondii)은 다양한 항원을 가지고 있으며, 이들 항원의 분석은 세 포매개성 면역반응 및 톡소포자충증의 면역학적 진단방법의 연구에 매우 중요하다 본 연구는 톡소포자충의 여러 단백질중 대부분의 충주(strain)에 존재하는 분자량 30 kDa의 단백질을 단클론 항체를 이용하여 분리한 후. 30 kDa 항원의 면역학적 특성을 초음파 추출 조항원과 비교 평가하였다. 톡소포자충의 세포막 항원으로 면역한 마우스 비장세포와 마우스 Sp2/0-Agl4 골수종세포를 융합하여 8개의 단클론 항체를 Western blot으로 확인하였다 이들 단클론 항체는 높은 특이성을 보였으며, $IgG_{2b}가{\;}5개,{\;}IgG^{1}이{\;}2개,{\;}IgG_{2a}$가 1개였다. 간접형광항체법으로 충체내 위치를 관찰한 결과. 30 kDa 항원은 tachyzoite의 표면 세포막에 주로 분포하였다. 단클론 항체와 CNBr-activated Sepharose 4B를 coupling하여 만든 immunoafrnity chromatography를 이용 하여 30 kDa 항원을 분리하였다. 분리한 30 kDa 항원으로 자극시킨 마우스 복강대식세포의 $NO_2^{-}$ 생산량은 초음파 추출 조항원 사용군에 비해 유의하게 증가하였으나 대식세포의 탐식능은 유의한 차이가 없었다. 또한 ELISA로 톡소포자충증을 진단시, 톡소포자충 30 kDa 항원 사용군은 조항원 사용군에 비해 민감도의 변화는 없었으나 특이성은 증가하였다 이상으로 보아 톡소포자충 30 kDa 항원은 감염 방어 면역 효과가 있었으며 진단에 이용시 특이성을 더 높일 수 있었다.