The Journal of Korean Society of Virology (대한바이러스학회지)

The Korean Society of Virology (대한바이러스학회)
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Dot Blot Assay for Screening of Anti-hantavirus Antibodies by Using Nucleocapsid Protein of Hantaan Virus

한탄바이러스 핵단백질을 이용한 항 한타바이러스 항체 검색용 Dot Blot Assay

  • Cho, Hae-Wol (Division of Arboviruses, Korea National Institute of Health) ;
  • Chung, Yeun-Jun (Department of Microbiology, Medical College, Catholic University) ;
  • Kim, Chung-Lim (Division of Arboviruses, Korea National Institute of Health) ;
  • Ban, Sang-Ja (Division of Arboviruses, Korea National Institute of Health) ;
  • Nam, Jae-Hwan (Division of Arboviruses, Korea National Institute of Health) ;
  • Lee, Hyeong-Woo (Division of Arboviruses, Korea National Institute of Health) ;
  • Lee, Yoo-Jin (Division of Arboviruses, Korea National Institute of Health) ;
  • Kim, Eun-Jung (Division of Arboviruses, Korea National Institute of Health)
  • 조해월 (국립보건원 신경계바이러스과) ;
  • 정연준 (가톨릭의대 미생물학교실) ;
  • 김정림 (국립보건원 신경계바이러스과) ;
  • 반상자 (국립보건원 신경계바이러스과) ;
  • 남재환 (국립보건원 신경계바이러스과) ;
  • 이형우 (국립보건원 신경계바이러스과) ;
  • 이유진 (국립보건원 신경계바이러스과) ;
  • 김은정 (국립보건원 신경계바이러스과)
  • Published : 1996.06.30
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Abstract

For easy and rapid screening of hemorrhagic fever with renal syndrome (HFRS) without any laboratory equipment, dot blot enzyme immunoassay was developed and tried to detect anti-hantavirus antibodies. The nucleocapsid protein of Hantaan virus was isolated by affinity chromatography and used for making the dot strip. 28 of 29 Hantaan virus infected sera showed positive signals and 21 of 22 HFRS negative sera showed no positive signals. Anti-Seoul virus monoclonal antibody also exibited positive signal but the intensity of colorization was approximately 5 fold less than that of anti-Hantaan monoclonal antibody. The sensitivity of dot blot assay was equal or superior to indirect immunofluorescent assay (IFA) or ELISA test. Overall, the screening results with dot blot assay showed 92.2 % of concordance with IFA or ELISA test. This results suggests that dot blot assay could be applied a tool for easy and rapid screening of HFRS.

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