Some structural characteristics of hot-water insoluble rice starch [3 varieties of Japonica type and 3 varieties of Tongil type(Japonica-Indica breeding type)]were investigated. The amylose contents of hot-water insoluble starches were 2.7-6.1%. The chain distributions of hot-water insoluble starches were composed of super long chain of above ${\overline{DP}}\;55$ (31.9-38.7%), B chain of ${\overline{DP}}\;40-50$ (12.3-18.0%) and A chain of ${\overline{DP}}\;10-20$ (48.5-50.4%). The chain distributions of hot-water insoluble starches and their ${\beta}-limit$ dextrin were difference between rice varieties and their were differ from those of amylopectin and their ${\beta}-limit$ dextrin. these result suggest that hot-water insolubility of rice starch was showed its characteristic on the varieties and it be responsible for the molecular structure of amylopectin.
The nitrogen (N) absorption and partition of the rice plants are important indicators that can be used to improve the N use efficiency (NUE) of the plants. Improving the plant NUE can help to avoid nutrient waste that may cause environmental pollution. To investigate the N absorption and partition of the rice plants, Hwaseongbyeo (Japonica) and Dasanbyeo (indica/japonica) were applied with N fertilizers at the rates of 60, 120, and 180 kg N per ha in paddy field. Also micro plots of $0.81m^2$ were established inside each plot for application of $^{15}N-labeled$ fertilizer. The differences in N utilization of the rice plants were associated with the total N absorption and partitioning after the heading stage. In the grain filling period, the increase of nitrogen content in the total and leaf blades of Dasanbyeo was higher than that of Hwaseongbyeo. Soil N was the main contributor for the increase of total N of Dasanbyeo during the grain filling period. The N fertilizer uptake rate of Hwaseongbyeo rapidly increased with the increment of N fertilization rate. In Dasanbyeo, N fertilizer uptakes were similar under all rates and times of N application. From heading stage to harvest, Dasanbyeo continued accumulating nitrogen, whereas Hwaseongbyeo had small changes. In conclusion, the difference in nitrogen absorption and partition after heading of the two cultivars was caused by the ability of Dasanbyeo to accumulate and remobilize soil nitrogen to the grains during the grain filling period.
Ngoc Ha Luong;Sangshetty G. Balkunde;Kyu-Chan Shim;Cheryl Adeva;Hyun-Sook Lee;Hyun-Jung Kim;Sang-Nag Ahn
Proceedings of the Korean Society of Crop Science Conference
/
2022.10a
/
pp.33-33
/
2022
Rice (Oryza sativa L.) is a widely studied domesticated model plant. Seed awning is an unfavorable trait during rice harvesting and processing. Hence, awn was one of the target characters selected during domestication. However, the genetic mechanisms underlying awn development in rice are not well understood. In this study, we analyzed the genes for awn development using a mapping population derived from a cross between the Korean indica cultivar 'Milyang23' and NIL4/9 (derived from a cross between 'Hwaseong' and O. minuta). Two quantitative trait loci (QTLs), qAwn4 and qAwn9 were mapped on chromosome 4 and 9, respectively, increased awn length in an additive manner. Through comparative sequencing analyses parental lines, LABA1 was determined as the causal gene underlying qAwn4. qAwn9 was mapped to a 199-kb physical region between markers RM24663 and RM24679. Within this interval, 27 annotated genes were identified, and five genes, including a basic leucine zipper transcription factor 76 (OsbZIP76), were considered candidate genes for qAwn9 based on their functional annotations and sequence variations. Haplotype analysis using the candidate genes revealed tropical japonica specific sequence variants in the qAwn9 region, which partly explains the non-detection of qAwn9 in previous studies that used progenies from interspecific crosses. This provides further evidence that OsbZIP76 is possibly a causal gene for qAwn9. The O. minuta qAwn9 allele was identified as a major QTL associated with awn development in rice, providing an important molecular target for basic genetic research and domestication studies. Our results lay the foundation for further cloning of the awn gene underlying qAwn9.
Zhang, Defu;Li, Defa;Piao, X.S.;Han, In K.;Yang, Chul J.;Shin, In S.;Dai, J.G.;Li, J.B.
Asian-Australasian Journal of Animal Sciences
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v.15
no.9
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pp.1334-1340
/
2002
A 4 week experiment was designed to study the effects of replacing corn with Chinese brown rice or adding different amylase in brown rice basal diet on growth performance and apparent fecal digestibilities of nutrients in growing pigs. One hundred and eight cross-bred pigs (Duroc${\times}$Landrace${\times}$Large White), weighing an average of $18.35{\pm}0.12kg$, were randomly assigned to 6 treatments with 6 replications per treatment. Diet in treatment 1 was corn-soybean meal basal diet, and in treatment 2, 3 and 4, corn was replaced by brown rice on rates of 33.3%, 66.7% and 100% respectively on the basis of treatment 1. And diets in treatment 5 and 6 were similar to treatment 4 except two kinds of amylases, glucoamylase and ${\alpha}$-amylase, were added respectively. The brown rice used in this experiment was husked from one kind of early, long grain, non-glutinous rice (ELGNR, indica rice) in southern China. The results indicated that there was a slight improvement in growth performance of pigs in brown rice treatments (p>0.05). The blood urea nitrogen value in treatment 2 was lower than that in treatment 1 (p<0.05). The differences of apparent fecal digestibilities of most nutrients were significant (p<0.05) except CP. Digestibilities of GE, OM and DM in treatment 4 were the best and digestibility of crude fat in treatment 5 appeared best (p<0.05). Contrast results between treatment 1 and treatment 2 to 4 indicated that the digestibility of GE, OM and DM increased significantly with the replacing rates of brown rice (p<0.05). Contrast results between treatment 4 and 5 indicated that adding glucoamylase in brown rice diet increased growth performance slightly (p>0.05) but not for digestibilities. This experiment shows a positive effect of brown rice on growth performance, especially on nutrient digestibility.
The relationship betwwen the molecular structure of amylopectin and the texture of cooked rice was investigated using Korean rice [3 varieties of Japonica type and 3 varieties of Tongil type(Japonica-Indica breeding type)]. The molecular structure of rice amylopectin was polymodal and distributed A chain of $\overline{DP}$ 12.4, short B chain of $\overline{DP}$ 20.6, B chain of $\overline{DP}$ 26.3, long B chain of $\overline{DP}$ 45 and super long chain of above $\overline{DP}$ 55. The super long chain of amylopectin was composed of long linear chain with poorly branched chain. Also, the super long chain of amylopectin showed positive correlated with average chain length, inherent viscosity and ${\beta}-amyloysis$ limit$({\%})$, but negative correlated with ${\lambda}max$ of iodine reaction of amylopectin. The structural properties of amylopectin in Japonica type were different from those of amylopectin in Tongil type. In relationship between molecular structure of amylopectin and texture of cooked rice, the average chain length, inherent viscosity, ${\beta}-amyloysis$ limit and super long chain of amylopectin was showed a positive correlation with hardness, but a negative correlation with adhesiveness of cooked rice. The long chain of rice amylopectin is the less, the eating quality of cooled rice was the better. These results suggest that the molecular structure of rice amylopectin could be responsible for the texture of cooked rice.
Experiments were carried out to evaluate the standard gravity in determining potential kernel size and to determine the effective sampling way by analyzing intra - and inter - plant variations for some source and sink characters using eleven semi-dwarf indica and three japonica cultivars including four semi-dwarf indica nearisogenic lines. Also, additional experiments were conducted to understand yearly variation and variety x year interaction effects for ten characters related to source and sink and to characterize the varietal difference of pre- and post-heading self-competition employing three parental varieties and their F$\sub$5/ progenies in 1982 and 1983. It is desirable to determine the potential kernel size by average kernel wight of rice grains showing above 1.15 specific gravity. There was significant difference in leaf area per tiller, spikelets and sink capacity per panicle among vigorous, intermediate and inferior tillers classified by differentiated order and vigorousness. Although it was difficult to find out any significant difference in grain-fill ratio, ratio of perfectly ripened grain, potential kernel size and sink/source ratio between vigorous and intermediate tillers, there was big difference between them and inferior one. The coefficients of variation within each tiller-group for some characters related to source and sink were larger with the order of vigorous tillers < intermediate one '||'&'||'lt; inferior one, and the average heritability of all characters, evaluated by the ratio of varietal variance (equation omitted) to total variance (equation omitted), were higher with the order of inferior tillers '||'&'||'lt; intemediate one '||'&'||'lt; superior one. Therefore, it is desirable to sample the vigorous tillers to represent the varietal difference of these traits. '82-'83 year variations of three parental cultivars were significant for all traits except for leaf area/tiller, panicles/hill, leaf area index and rough rice yield. The characters showing highly significant variance of variety x year interaction were growth duration from transplanting to heading, leaf area/tiller, sink/source ratio, sink capacity/panicle and grain yield. Generalized yearly response of three parental varieties (Suweon 264, Raegyeong, IR1317-70-l) and their F$\sub$5/ progenies on the 1st and 2nd principal components extracted from ten source and sink characters generally exhibited reduction in both source and sink. However, there were diverse variety x year interactions such as progenies showing similar reaction with their parents and intermediate or recombinational yearly response with little or considerable yearly movement on the four-dimensional planes of the two upper principal components between 1982 and 1983. Sink characters revealing highly significant border effect were grain-fill ratio, spikelets and sink capacity per panicle. Among them the latter two especially showed significant variety x border effect interaction. Self-competition characterized by relative weakness of inside plant's sink characters compared to the border one was more severe during the reproductive stage before heading than maturing stage. Though the larger sink capacity per panicle generally disclosed the severer self-competition, some lines (like Suweon 264) revealed severe self-competition with small sink capacity while a few others showed tender self-competition in spite of big sink capacity per panicle.
Korean Journal of Agricultural and Forest Meteorology
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v.3
no.1
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pp.22-29
/
2001
In Korean high yielding varieties developed by crosses between indica and japonica rice, the most limiting factor for yield may be attributed to the less number of the tillers in the unit area. The goals of this study is to find out the effect of the temperature factors as well as cultural practices on the development and increase of tillers of Dasanbyeo, the high yielding indica crossed japonica cultivar. The effect of temperature was examined under controlled phytotron condition with 6 levels of temperature, 15, 17, 19, 22, 24 and 26$^{\circ}C$, respectively, For the experiment, the leading japonica variety in Korea, Hwaseongbyeo, was used for the check cultivar for the comparison with Dasanbyeo. The high temperature also accelerated the initiation and termination of tiller development. The cultivar difference in the speed of tiller development was observed, for example, more rapid development of tiller in Dasanbyeo than in Hwaseongbyeo was observed at the high temperature range of 24-26$^{\circ}C$, while the vice versa phenomena was observed at lower temperature range of 17-22$^{\circ}C$. The first secondary tiller of Dasanbyeo was observed on the 16, 17, 23, 27 and 38 days after transplanting (DAT) at 26$^{\circ}C$, 24$^{\circ}C$, 22$^{\circ}C$, 19$^{\circ}C$, and 17$^{\circ}C$, respectively. Those of Hwaseongbyeo was 19-22, and 26 DAT at 19-26$^{\circ}C$ and 17$^{\circ}C$, respectively. The last effective tiller of Dasanbyeo was observed on 27-33 DAT for the primary tiller, 20-41 DAT for the secondary tiller. Those of Hwaseongbyeo were 23-40 DAT for primary tiller, and 24-40 DAT for the secondary tiller, and 24-40 DAT for the secondary tiller.
Korean Journal of Agricultural and Forest Meteorology
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v.4
no.3
/
pp.151-158
/
2002
In Korean high yielding varieties developed by crosses between indica and japonica rice, the most limiting factor for yield increase may be attributed to the less number of tillers per unit area. The goal of this study is to find out the effect of the environmental factors as well as cultivation practice on the development and increase of tillers of Dasanbyeo, the high yielding indica / japonica hybrid cultivar. The effect of temperature was examined with 3 different light intensity, 220,600, and 1220 $\mu$㏖/s/$m^2$, respectively. For all the experiments, the leading japonica variety in Korea, Hwaseongbyeo, was used fur the check cultivar for the comparison with Dasanbyeo. The increase of the tillers was more prominent in Dasanbyeo than in Hwaseongbyeo at 220 $\mu$㏖/s/$m^2$ of light intensity, while the similar increase of tiller no. was found at 600 $\mu$㏖/s/$m^2$ of light intensity treatment. However, Hwaseongbyeo showed more rapid increase of tiller at 1220 $\mu$㏖/s/$m^2$ of light intensity. The mean number of the primary tiller ranged 5 to 7 in Dasanbyeo, and 2 to 7 in Hwaseongbyeo, showing greater variation in the latter case. However, the secondary tiller number ranged from 2 to 13 for the former, and 2 to 12 for the latter. The earliest initiation of tiller node of Dasanbyeo and Hwaseongbyeo was observed on 6 and 4 days after transplanting(DAT), respectively, at 600 $\mu$㏖/s/$m^2$ of light intensity, while 10, and 7 DAT, respectively, at 1,220 $\mu$㏖/s/$m^2$. No cultivar difference was observed at 600 $\mu$㏖/s/$m^2$ with the 18 DAT. The ratio of effective tiller was lower in Dasanbyeo, ranging from 47 to 55% than in Hwaseongbyeo, ranging from 72 to 100%.
Attempts to search infection period, infection speed in the tissue of neck blast of rice plant, location of inoculum source and effects of several conditions about the leaf sheath of rice plants for neck blast incidence have been made. 1. The most infectious period for neck blast incidence was the booting stage just before heading date, and most of necks have been infected during the booting stage and on heading date. But $Indica{\times}Japonica$ hybrid varieties had shown always high possibility for infection after booting stage. 2. Incubation period for neck blast of rice plants under natural conditions had rather a long period ranging from 10 to 22 days. Under artificial inoculation condition incubation period in the young panicle was shorter than in the old panicle. Panicles that emerged from the sheath of flag leaf had long incubation period, with a low infection rate and they also shown slow infection speed in the tissue. 3. Considering the incubation period of neck blast of rice plant, we assumed that the most effective application periods of chemicals are 5-10 days for immediate effective chemicals and 10-15 days for slow effective chemicals before heading. 4. Infiltration of conidia into the leaf sheath of rice plant carried out by saturation effect with water through the suture of the upper three leaves. The number of conidia observed in the leaf sheath during the booting stage were higher than those in the leaf sheath during other stages. Ligule had protected to infiltrate of conidia into the leaf sheath. 5. When conidia were infiltrated into the leaf sheath, the highest number of attached conidia was observed on the panicle base and panicle axis with hairs and degenerated panicle, which seemed to promote the infection of neck blast. 6. The lowest spore concentration for neck blast incidence was variable with rice varietal groups. $Indica{\times}Japonica$ hybrid varieties were infected easily compared to the Japonica type varieties, especially. The number of spores for neck blast incidence in $Indica{\times}Japonica$ hybrid varieties was less than 100 and disease index was higher also in $Indica{\times}Japonica$ hybrid than in Japonica type varieties. 7. Nitrogen content and silicate content were related with blast incidence in necks of rice plants in the different growing stage changed during growing period. Nitrogen content increased from booting stage to heading date and then decreased gradually as time passes. Silicate content increased from booting stage after heading with time. Change of these content promoted to increase neck blast infection. 8. Conidia moved to rice plant by ascending and desending dispersal and then attached on the rice plant. Conidia transfered horizontally was found very negligible. So we presumed that infection rate of neck blast was very low after emergence of panicle base from the leaf sheath. Also ascending air current by temperature difference between upper and lower side of rice plant seemed to increase the liberation of spores. 9. Conidial number of the blast fungus collected just before and after heading date was closely related with neck blast incidence. Lesions on three leaves from the top were closely related with neck blast incidence, because they had high potential for conidia formation of rice blast fungus and they were direct inoculum sources for neck blast. 10. The condition inside the leaf sheath was very favorable for the incidence of neck blast and the neck blast incidence in the leaf sheath increased as the level of fertilizer applied increased. Therefore, the infection rate of neck blast on the all panicle parts such as panicle base, panicle branches, spikelets, nodes, and internodes inside the leaf sheath didn't show differences due to varietal resistance or fertilizers applied. 11. Except for others among dominant species of fungi in the leaf sheath, only Gerlachia oryzae appeared to promote incidence of neck blast. It was assumed that days for heading of varieties were related with neck blast incidence.
A total of 16 varieties from Korea and Indonesia were tested at Suwon, Korea (126$^{\circ}$19'E, 37$^{\circ}$16'N and 37m above sea level) and Bali, Indonesia (115$^{\circ}$ 14'E, 8$^{\circ}$42'S and 10m above sea level). Japonica-type varieties showed pre-matured heading at Bali, tropical zone, while Bulu varieties showed no heading at Suwon, temperate zone. The varieties of Indica-type and Ind./Jap. showed faster heading in tropical zone than in temperate zone. Dry matter of the varieties weighed more difference among varietal groups in tropical than in temperate area, while plant height responsed differently depending on variety and test region.
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