• Proceedings of the National Institute of Ecology of the Republic of Korea
• /
• v.2 no.2
• /
• pp.120-128
• /
• 2021

The objective of this study was to analyze the genotype of black-spotted pond frog (Pelophylax nigromaculatus) using seven microsatellite loci to quantify its genetic diversity and population structure throughout the spatial scale of basins of Han, Geum, Yeongsan, and Nakdong Rivers in South Korea. Genetic diversities in these four areas were compared using diversity index and inbreeding coefficient obtained from the number and frequency of alleles as well as heterozygosity. Additionally, the population structure was confirmed with population differentiation, Nei's genetic distance, multivariate analysis, and Bayesian clustering analysis. Interestingly, a negative genetic diversity pattern was observed in the Han River basin, indicating possible recent habitat disturbances or population declines. In contrast, a positive genetic diversity pattern was found for the population in the Nakdong River basin that had remained the most stable. Results of population structure suggested that populations of black-spotted pond frogs distributed in these four river basins were genetically independent. In particular, the population of the Nakdong River basin had the greatest genetic distance, indicating that it might have originated from an independent population. These results support the use of genetics in addition to designations strictly based on geographic stream areas to define the spatial scale of populations for management and conservation practices.

• Journal of Microbiology and Biotechnology
• /
• v.31 no.11
• /
• pp.1481-1489
• /
• 2021

Early and accurate detection of pathogens is important to improve clinical outcomes of bloodstream infections (BSI), especially in the case of drug-resistant pathogens. In this study, we aimed to develop a culture-independent digital PCR (dPCR) system for multiplex detection of major sepsis-causing gram-negative pathogens and antimicrobial resistance genes using plasma DNA from BSI patients. Our duplex dPCR system successfully detected nine targets (five bacteria-specific targets and four antimicrobial resistance genes) through five reactions within 3 hours. The minimum detection limit was 50 ag of bacterial DNA, suggesting that 1 CFU/ml of bacteria in the blood can be detected. To validate the clinical applicability, cell-free DNA samples from febrile patients were tested with our system and confirmed high consistency with conventional blood culture. This system can support early identification of some drug-resistant gram-negative pathogens, which can help improving treatment outcomes of BSI.

• Journal of Korea Water Resources Association
• /
• v.45 no.7
• /
• pp.713-727
• /
• 2012

In this study, the bivariate frequency analysis of the independent annual rainfall event series was done to be used for the runoff analysis, whose results were also compared with those from the conventional univariate frequency analysis. This study was applied to three differently-sized basins such as the Joongryang Stream, Chunggye Stream, and Ooyi Stream. The Clark model was used as the runoff model, and the SCS method was applied for the calculation of the effective rainfall. The alternating block method and the Huff method were considered to be compared for the temporal distribution of rainfall event. Summarizing the results are as follows. (1) The difference between the univariate and bivariate frequency analysis results were large when the rainfall duration was short, but significantly decreased as the rainfall duration increased. The univariate frequency analysis results were bigger when the rainfall duration was short, but smaller in opposite case. (2) The peak flow derived by applying the alternating block method was bigger than that by the Huff method. Also, the peak flow when applying the alternating block method increased as the rainfall duration increased, but converged smoothly around the rainfall duration of 24 hours. (3) For the Joongryang Stream, when applying the Huff method, the peak flow derived for the bivariate frequency analysis was bigger than that for the univariate case, but for the other two basins, the results were opposite. When applying the alternating block method, the results were consistent for all three basins that the peak flow derived by applying the bivariate frequency analysis was bigger than those by the univariate frequency analysis.

• Journal of East-West Nursing Research
• /
• v.4 no.1
• /
• pp.66-76
• /
• 1999

The auricular acupuncture therapy is stimulus treat method that acupuncts or moxibusts at the reaction blood vessel point of various diseases in the upper portion of ear. And this effects from the reaction blood vessel point of an ear to the meridian of whole body system through blood stream. The auricular acupuncture has cured and cared signs and symptoms of many diseases and pathologic problems. And this treatment heels abnormal reactions, nursing focuses so it was examined through literature review that nurses use many portions of the auricular acupuncture in their clinical cares of oriental medicine. This study was proved that the Mae-Chim acupuncture method among various methods of the auricular acupuncture is easy and simple so this method can be used by nurses. And the ear acupuncture site which controls diarrhea, insomnia, migraine, stress vomiting, dyspepsia, high fever, insufficient lactation, bowel dysfunction, menoxenia, anxiety, pruritus and can be found easily and is few the number of acupuncture so the oriental medicine nurses can apply easily to their clients. Therefore the independent oriental medicine nursing intervention can be developed by application of the auricular acupuncture therapy on the oriental medicine clinical nursing and home care in our community.

• Journal of the Korea Furniture Society
• /
• v.26 no.3
• /
• pp.241-251
• /
• 2015

Milano Fair is the most authoritative event in the world and the most noticeable exhibition for expecting stream of furniture design and future. Companies and designers, who participate in this event, pioneer independent market through the creative and creative products based on their own identity. Their competitiveness in the market comes from the identity of the brands and it is possible when making continuous efforts to make competitiveness by differentiated designs and products. Upright design culture will be made when respecting the value of designers and designs, making the history and tradition and striving for new challenges and pioneerings.

• Journal of the korean Society of Automotive Engineers
• /
• v.5 no.3
• /
• pp.33-44
• /
• 1983

This paper deals with the experimental study on the behavior of fuel (methanol) in intake manifold by using the basic apparatus which is manufactured the visible straight tube type model. In this study, the new device for liquid film thickness measurement and vaporization rate measurement are introduced to investigate the variation of liquid film thickness along the intake manifold and to observe the effect of vaporization of injected fuel. the results are summarized as follows: 1) The vaporization rate increases in proportion to decreasing of throttle valve angle and growing air fuel ratio. 2) The liquid film thickness along the intake manifold is mostly independent for the throttle valve angle in low air velocity and then affected in high air velocity, but the distribution of the liquid film thickness on circumferential position almost constant in the region of 300mm down stream from carburetor. 3) The mean liquid film thickness is 0.04 - 0.18mm in case of methanol in the region of air velocity Va = 12m/s - 55m/s and decreases with decreasing the throttle valve angle.

• Journal of the Korean Mathematical Society
• /
• v.38 no.4
• /
• pp.807-842
• /
• 2001

This paper summarizes recent development of analytical and algorithmical results for stationary FIFO queues with multiple Markovian arrival streams, where service time distributions are general and they may differ for different arrival streams. While this kind of queues naturally arises in considering queues with a superposition of independent phase-type arrivals, the conventional approach based on the queue length dynamics (i.e., M/G/1 pradigm) is not applicable to this kind of queues. On the contrary, the workload process has a Markovian property, so that it is analytically tractable. This paper first reviews the results for the stationary distributions of the amount of work-in-system, actual waiting time and sojourn time, all of which were obtained in the last six years by the author. Further this paper shows an alternative approach, recently developed by the author, to analyze the joint queue length distribution based on the waiting time distribution. An emphasis is placed on how to construct a numerically feasible recursion to compute the stationary queue length mass function.

• Journal of Korea Multimedia Society
• /
• v.15 no.6
• /
• pp.693-700
• /
• 2012

The effective extraction of key frames from a video stream is an essential task for summarizing and representing the content of a video. Accordingly, this paper proposes a new and fast method for extracting key frames from a compressed video. In the proposed approach, after the entire video sequence has been segmented into elementary content units, called shots, key frame extraction is performed by first assigning the number of key frames to each shot, and then distributing the key frames over the shot using a probabilistic approach to locate the optimal position of the key frames. The main advantage of the proposed method is that no time-consuming computations are needed for distributing the key frames within the shots and the procedure for key frame extraction is completely automatic. Furthermore, the set of key frames is independent of any subjective thresholds or manually set parameters.

• Proceedings of the KSRS Conference
• /
• 2008.10a
• /
• pp.406-409
• /
• 2008

Clustering data streams has an importance over many applications like sensor networks. Existing hierarchical methods follow a semi fuzzy clustering that yields duplicate clusters. In order to solve the problems, we propose an extended online divisive agglomerative clustering on data streams. It builds a tree-like top-down hierarchy of clusters that evolves with data streams using geometric time frame for snapshots. It is an enhancement of the Online Divisive Agglomerative Clustering (ODAC) with a pruning strategy to avoid duplicate clusters. Our main features are providing update time and memory space which is independent of the number of examples on data streams. It can be utilized for clustering sensor data and network monitoring as well as web click streams.

• 한국전산유체공학회:학술대회논문집
• /
• 2005.04a
• /
• pp.228-233
• /
• 2005

Two-dimensional Stokes flows through a micro channel with a couple of symmetric vertical fins are investigated. At far up- and down-stream from the fins, the plane Poiseuille flow exists in the channel. The slip boundary conditions are applied to take account of the Knudsen number effects. For the analysis, the method of eigen function expansion and collocation method are employed. By the results, the streamline patterns and pressure distributions are shown and the force exerted on the fin and the excess pressure drop due to the fins are determined as functions of the length of the fin and Knudsen number. It may be conjectured that the force and the excess pressure drop are almost independent of the Knudsen number.