• KSBB Journal
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• 제11권1호
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• pp.8-14
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• 1996

Bacillus subtilis를 재조합이종 단백질 생산에 응 용하고 그 발현효율의 향상을 위하여, aprE 프로모 터를 모델계로서 이에 작용하는 전이상태 조절유전 자 변이와 포자형성변이의 영향을 살펴보았다. 사용 된 균주는 두 개의 프로테아제가 제거된 DB104이며, 조절인자 변이주는 $degU^h$와 hpr을 각각 활성인자와 억제인자로 선택하였고, 이중변이주는 DB104 ($\DeltaspoIIG(Pm) degU^h his^+$) 및 DB104($\DeltaspoIIG(Pm) \Deltahpr(Em)$) 를 제조하였다. 이에 목적유전 자연 aprE유전자를 재조합하여 변이에 의한 과발현 양상을 확인하였다. degUh 및 hpr 조절변이주는 야 생주와 비교하여 각각 약 7배 빛 약 2배의 발현효율 향상을 보여주었다. 이들 전이상태 조절유전자 변이 는 모두 목적유전자 복제 수의 증가에 따라 발현효 율의 감소를 나타내어 증폭된 목적유전자에 대한 조 절유전자의 희석효과(gene dosage effect)를 확인할 수 있었다. 이중변이주 DB104($\DeltaspoIIG(Pm) degU^h his^+$)::pMK101 및 DB104($\DeltaspoIIG(Pm) \Deltahpr(Em)$)::pMK101에서의 aprE 발현은 각각 약 10배 빛 3배의 발현증가를 가져와 aprE 발현에 대한 포자변이주 및 전이상태 조절유전자의 독립적인 부가효과를 확인하였다.

• Journal of Yeungnam Medical Science
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• 제30권2호
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• pp.83-89
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• 2013

Background: The prevalence of extended-spectrum ${\beta}$-lactamase (ESBL)-producing Escherichia coli is increasing rapidly worldwide. Treatment options for ESBL-producing E. coli are limited, and infections caused by this organism are associated with improper antibiotic use, a long hospital stay, and increased mortality. Thus, the assessment and early recognition of the risk factors of nosocomial infections due to ESBL-producing E. coli are important for the infection control and proper treatment. Methods: A case-control study was performed that included nosocomial episodes of ESBL-producing E. coli bacteremia at a tertiary care hospital from January 2004 to December 2007. For each case patient, three controls were randomly selected and data on predisposing factors were collected. Results: Fifty-five cases of nosocomial ESBL-producing E. coli bacteremia were studied. Carbapenem usage (OR: 11.3, 95% CI: 1.1-115.9, p=0.041), quinolone usage (OR: 4.5, 95% CI: 1.1-18.8, p=0.042), biliary obstructive disease (OR: 11.8, 95% CI: 3.0-46.7, p<0.001) and the APACHE II score (OR: 1.3, 95% CI: 1.2- 1.5, p<0.001) were analyzed as independent risk factors of nosocomial ESBL-producing E. coli bacteremia. Conclusion: Our results showed that physicians caring for patients with risk factors of nosocomial bacteremia should consider ESBL-producing E. coli as the causative organisms of the disease.

• 한국대기환경학회지
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• 제23권4호
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• pp.420-429
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• 2007

In this study, we attempted to evaluate the relationship between air dilution sensory test and instrumental detection method for individual odorous compound. For the purpose of our comparative study, we conducted the analysis of malodor on total of 46 samples collected from both industrial and food waste sectors using the two independent approaches. The results of instrumental analyses were explained after modification to several parameters such as: the sum of odor concentration (SOC), sum of odor quotient (SOQ) and sum of odor intensity (SOI). When we compared dilution ratio values (DRV) of threshold limit (TL) with the modified instrumental data sets, pearson coefficient of SOC, SOQ, and SOI were 0.556 (p=5.83E-05), 0.911 (p=9.64E-19) and 0.847 (p=8.93E-14), respectively. The results of this study thus suggest that the DRV of TL sensitively reflects odor strength measured intuitive.

• Journal of Gastric Cancer
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• 제4권2호
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• pp.82-88
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• 2004

Purpose: E-cadherin and CD44H have been shown to play a role in the progression and the metastasis of tumors. This study evaluated the clinical correlations between expression of E-cadherin and CD44H and various clinicopathologic factors and the value of expressions of E-cadherin and CD44H as prognostic factors in Borrmann type IV gastric cancer. Materials and Methods: In 122 patients with Borrmann type IV gastric cancer, we performed the immunohistochemical stainings for E-cadherin and CD44H. We analyzed the correlation between the expressions of E-cadherin and CD44H and lymphatic invasion, venous invasion, perineural invasion, histologic type, lymph node metastasis, depth of invasion, stage, and peritoneal dissemination, and survival. Results: There were no correlations between reduced expression of E-cadherin and CD44H and lymphatic invasion, venous invasion, perineural invasion, histologic type, lymph node metastasis, depth of invasion, and stage. However, there was a significant correlation between lymph node metastasis and the lymphatic invasion (P=0.022). There was also a significant correlation between the peritoneal dissemination and CD44H expression (P=0.005). The 5-year survival rate was correlated with CD44H expression expression (P=0.026), peritoneal dissemination (P<0.01), depth of invasion (P<0.01), lymph node metastasis (P<0.01), stage of tumor (P<0.01), and lymphatic invasion (P<0.01). There was no correlation between expression of E-cadherin and survival rate. Conclusion: The expression of CD44H and peritoneal dissemination was correlated. The expression of CD44H was an independent prognostic factor in Borrmann type IV gastric cancer. Further prospective studies with a large number of cases are required.

• 대한수학회논문집
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• 제9권4호
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• pp.927-931
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• 1994

Let G be a cyclic group of order 2 and let $S^1$ denote the unit circle in $R^2$ with the standard metric. We consider smooth G-vector bundles over $S^1$ when G acts on $S^1$ by reflection. Then the fixed point set of G on $S^1$ is two points ${z_0, z_1}$. Let $E$\mid$_{z_0} and E$\mid$_{z_1}$ be the fiber G-representation spaces at $z_0$ and $z_1$ respectively. We associate an orthogonal G-representation $\rho_i : G \to O(n)$ to $E$\mid$_{z_i}, i = 0, 1$. Let det $p\rho_i(g), g \neq 1$, be denoted by det $E$\mid$_{z_i}$ since det $\rho_i(g)$ is independent of choice of $\rho_i$.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1998년도 하계학술대회 논문집 A
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• pp.390-392
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• 1998

Recently, the interest of the high speed railway train is increased. Because it is energy-conserving and largely independent of fuel oil, an electric train is environmental friendly. In this paper we designed the eddy current brake for simulator of the Korean High Speed Railway Train(hereafter KHRT). Braking force and normal force according to current and speed are calculated.

• 한국산학기술학회논문지
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• 제18권9호
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• pp.113-122
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• 2017

본 연구는 대학생의 생의 의미와 행복 정도를 확인하고 이들 변수간의 관계를 파악하며 행복에 영향을 미치는 요인을 규명하기 위한 서술적 조사연구이다. 연구대상자는 경상남도에 소재하는 일개 대학의 재학생 205명을 대상으로 하였다. 자료수집기간은 2016년 10월 10일부터 10월 30일까지였고, 수집된 자료는 빈도와 백분율, independent t-test, one-way ANOVA, $Scheff{\acute{e}}$ test, Cronbach's alpha 계수, Pearson's correlation coefficient 및 multiple regression으로 분석하였다. 연구결과 대상자의 행복 점수는 6점 만점에 평균 4.18점, 생의 의미는 5점 만점에 평균 3.73점이었다. 대상자의 일반적 특성에 따른 행복 수준은 성별(p=.045), 학년(p=.038), 전공만족도(p<.001), 건강상태(p<.001)에 따라 차이가 있었다. 대상자의 행복은 하위요인인 자신감(p<.001), 자기통제(p<.001), 긍정적 정서(p<.001)와 생의 의미(p<.001), 생의 의미발견(p<.001), 생의 의미추구(p<.001)와 유의한 양적 상관관계를 보였다. 대상자의 행복에 영향을 미치는 요인은 생의 의미발견, 건강상태, 전공만족도가 유의하였고, 전체 설명력은 41%였다. 따라서 생의 의미는 행복에 밀접한 관련성이 있으며, 가장 긍정적인 영향을 미치는 요인임을 확인하였기에 대학생의 행복강화를 위한 전략 개발에 생의 의미를 높이는 전략이 고려될 것으로 사료된다.

• Journal of Gastric Cancer
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• 제21권3호
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• pp.236-245
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• 2021

Purpose: The numeric N stage has replaced the topographic N stage in the current tumor node metastasis (TNM) staging in gastric carcinoma. However, the usefulness of the topographic N stage in the current TNM staging system is uncertain. We aimed to investigate the prognostic value of the topographic N stage in the current TNM staging system. Materials and Methods: We reviewed the data of 3350 patients with gastric cancer who underwent curative gastrectomy. The anatomic regions of the metastatic lymph nodes (MLNs) were classified into 2 groups: perigastric and extra-perigastric. The prognostic value of the anatomic region was analyzed using a multivariate prognostic model with adjustments for the TNM stage. Results: In patients with lymph node metastasis, extra-perigastric metastasis demonstrated significantly worse survival than perigastric metastasis alone (5-year survival rate, 39.6% vs. 73.1%, respectively, P<0.001). Extra-perigastric metastasis demonstrated significantly worse survival within the same pN stage; the multivariate analysis indicated that extra-perigastric metastasis was an independent poor prognostic factor (hazard ratio=1.33; 95% confidence interval=1.01-1.75). The anatomic region of the MLNs improved the goodness-of-fit (likelihood ratio statistics, 4.57; P=0.033) of the prognostic model using the TNM stage. Conclusions: The anatomic region of MLNs has an independent prognostic value in the numeric N stage in the current TNM staging of gastric carcinoma.

• Journal of Photoscience
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• 제9권2호
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• pp.229-232
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• 2002

There are two distinct UV-responsive signaling pathways in UV-irradiated mammalian cells, i.e., the DNA damage-dependent and -independent pathways. The former occurs in nucleus and results in growth arrest and apoptosis via post-translational modification of p53. The latter is initiated by oxidative stress and/or by damages in cell membrane or cytoplasm, which activate signaling cascade through intracellular molecules including mitogen activated protein kinases (MAPK). In normal human fibroblastic cells, all of MAPK family members, extracellular signal-related kinases (ERK), c-Jun N-terminal kinases (JNK) and p38, were rapidly phosphorylated following UV-irradiation. ERK phosphorylation was suppressed by an inhibitor of receptor tyrosine kinases (RTK). As ERK usually responds to mitogenic stimuli from RTK ligands, UV-induced ERK phosphorylation may be linked to the proliferation of survived cells. In contrast, phosphorylation of JNK and p38, as well as apoptosis, were modulated by the level of UV-generated oxidative stress Therefore, JNK and p38 may take part in oxidative stress-mediated apoptosis. Phosphorylation of p53 at Ser and Thr residues are essential for stabilization and activation of p53. Among several sites reported, we confirmed phosphorylation at Ser-15 and Ser-392 after UV-irradiation. Both of these were inhibited by a phosphoinositide 3-kinase inhibitor, presumably due to the shutdown of signals from DNA damage to p53. Phosphorylation at Ser-392 was also sensitive to an antioxidant and a p38 inhibitor, suggesting that Ser-392 of p53 is one of the possible points where DNA damage-dependent and -independent apoptic signals merge. Thus, MAPK pathway links UV-induced intracellular signals to the nuclear responses and modifies DNA damage-dependent cellular outcome, resulting in the determination of cell death.

• 한국문헌정보학회지
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• 제41권3호
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• pp.55-79
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• 2007

본 연구는 도서관 정보서비스 패러다임의 변화에 따른 새로운 서비스품질 평가 척도의 필요성을 이해하고, 디지털도서관에 적용할 수 있는 e-서비스품질 평가 차원과 모형을 개발하는 연구이다. 이론 연구를 통해 개발된 차원과 모형을 검정하기 위해 실제 NDSL 정보시스템을 적용하여 개발된 차원과 이를 구성하는 선행요인을 분석하였으며, 제안한 e-서비스품질 모형을 토대로 변인간의 인과적 관계를 분석하였다. 아울러 서비스품질에 대한 고객의 이상적 기대치와 실제 지각된 성과치 간의 갭(차이)을 분석하고 마지막으로 고객 관점에서 가장 중요하게 생각하는 디지털도서관 서비스품질 요소가 무엇인지 알아보았다.