• Journal of Life Science
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• v.27 no.11
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• pp.1349-1356
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• 2017

Potassium channel openers (KCOs) produce physiological and pharmacological defense mechanisms against cell injuries caused by oxidative stress of diverse origins. Openings of mitochondrial and plasmalemmal $K^+$ channels are involved in the defense mechanisms. This study tested whether NS 1619, an opener of large-conductance BK channels, has a similar beneficial influence on the pigment epithelial cells of retinas. The human retinal pigment epithelial cell line ARPE-19 was exposed to $H_2O_2$-induced oxidative stress in the absence and presence of NS 1619. The degrees of the cells' injuries were assessed by analyzing the cells' trypan-blue exclusion abilities and TUNEL staining. NS 1619 produced remarkable protections against cell injuries caused by $H_2O_2$. It prevented apoptotic and necrotic cell deaths. The protective effect of NS 1619 was significantly diminished when the cells were treated with NS 1619 in combination with the BK channel-blocker paxilline. NS 1619 significantly ameliorated cellular ATP deprivations in $H_2O_2$-treated cells. It helped mitochondria preserve their functional integrity, which was estimated by their MTT reduction abilities and mitochondrial membrane potential. In conclusion, it was suggested that NS 1619 had a beneficial effect on mitochondria in regards to preserving their functional integrity under oxidative stress, and it produces defense mechanisms against oxidant-induced cell injuries in ARPE-19 cells.

• Journal of Soil and Groundwater Environment
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• v.10 no.6
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• pp.20-31
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• 2005

Three dimensional Monte-Carlo simulations of non-ergodic transport of a lion-reactive solute plume by steady-state groundwater flow under a uniform mean velocity in isotropic heterogeneous aquifers were conducted. The log-normally distributed hydraulic conductivity, K(x), is modeled as a random field. Significant efforts are made to reduce tile simulation uncertainties. Ensemble averages of the second spatial moments of the plume and plume centroid variances were simulated with 1600 Monte Carlo runs for three variances of log K, ${\sigma}_Y^2=0.09,\;0.23$, and 0.46, and three dimensionless lengths of line plume sources normal to the mean velocity. The simulated second spatial moment and the plume centroid variance in longitudinal direction fit well to the first order theoretical results while the simulated transverse moments are generally larger than the first order results. The first order theoretical results significantly underestimated the simulated dimensionless transverse moments for the aquifers of large ${\sigma}_Y^2$ and large dimensionless time. The ergodic condition for the second spatial moments is far from reaching in all cases simulated, and transport In transverse directions may reach ergodic condition much slower than that in longitudinal direction. The evolution of the contaminant transported in a heterogeneous aquifer is not affected by the shape of the initial plume but affected mainly by the degree of the heterogeneity and the size of the initial plume.

• Journal of Food Hygiene and Safety
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• v.21 no.3
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• pp.171-180
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• 2006

Peroxisome proliferatorr-activated receptor-gamma $(PPAR{\gamma})$ must form a heterodimer with the retinoid-X receptor (RXR) to bind DNA, and its transcriptional activity is thought to be maximized by ligands specific for either receptor. Activated $(PPAR{\gamma})$ and $(PPAR{\gamma})$ ligands may influence tumor growth through regulation of the tumor suppressor PTEN. Our aim in this study was to determine whether co-stimulation with the $(PPAR{\gamma})$ ligand, ciglitazone, and RXR ligand can synergistically upregulate PTEN in human acute promyelocytic leukemia (APL) cells and consequently potentate the inhibition of cell growth and cell cycle progression of these cells. Human leukemia cell line, HL-60 cells were exposed to all-trans-retinol and ciglutazone. The PTEN expression was measured as the level of PTEN mRNA expression by RT-PCR and as the level of PTEN expression by western blot analysis. Cell cycle analysis was carried out by a propidium iodide (PI) staining method and analyzed with a FACScan. The $(PPAR{\gamma})$ ligand, ciglitazone, and the RXR ligand, retinoic acid, upregulated PTEN expression by HL-60 cells in time- and dose-dependent manners, respectively. This was significantly enhanced by a combination of both ciglitazone and retinoic acid. Moreover, these compounds synergistically induced arrests of both cell growth and the $G_l$ phase of the cell cycle. Thus, the activation of the $(PPAR{\gamma})$:RXR heterodimer may represent a regulatory pathway for human leukemia cells and there may be important roles for $(PPAR{\gamma})$ and RXR ligands in prophylactic and therapeutic approaches fur controlling leukemia through the upregulation of PTEN.

• Development and Reproduction
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• v.11 no.3
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• pp.219-226
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• 2007

This study was conducted to identify optimistic primordial germ cells'(PGCs) migration activity using heat activated busulfan treatment for the increasing germline chimerism. Donar PGCs viability tests of important conditions for useful germ line chimerism indicated approximately $70{\sim}80%$ viability was time dependent. Transplantation experiments of PGCs into recipient embryos after busulfun treatment, showed the treatment group having 23.5% viability. By comparison, the control group showed 4.8% viability. The 96 hour treatment group and the 118 hour treatment group of the cultured PGCs showed high migration activity. Generally, the transplantation method would consider morphological and physiological characteristics before transplantation. In the present study, the effect of busulfan on migration activity showed viability highest at 53.4% after 48-hour incubation time. However, a previous study showed the best condition for transplantation time to be prior to the 48-hour incubation period, when the chicken embryo does not yet have a developed blood vessel system. In conclusion, an important condition for the production of a transgenic chicken is that most donor PGCs migrate into the recipient embryo without any inhibitory factors. The present results suggest, perhaps by using this modified method of transplantation, it can produce a more efficient chimeric germ line, transgenic chicken.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.39 no.5
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• pp.544-555
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• 2002

The aim of this study is to develop the $^1H$-MRS data postprocessing software for both single-voxel and multi-voxel technique, which plays and important role as a diagnostic tool in clinical field. This software is based on graphical user interface(GUI) under windows operating system of personal computer(PC). In case of single-voxel MRS, both of raw data in time-domain and spectrum data in frequency-domain are simultaneously displayed in a screen. Several functions such as DC correction, zero filling, line broadening, Lorentz-Gauss filtering and phase correction, etc. are included to increase the quality of spectrum data. In case of multi-voxel analysis, spectroscopic image reconstructed by 3-D FFT was displayed as a spectral grid and overlapped over previously obtained T1- or T2-weighted image for the spectra to be spatially registered with the image. The analysis of MRS peaks were performed by obtaining the ratio of peak area. In single-voxel method, statistically processed peak-area ratios of MRS data obtained from normal human brain are presented. Using multi-voxel method, MR spectroscopic image and metabolite image acquired from brain tumor are demonstrated.

• Korean Journal of Plant Resources
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• v.32 no.2
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• pp.124-143
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• 2019

This study was carried out to build a database system for amylose and protein contents of rice germplasm based on NIRS (Near-Infrared Reflectance Spectroscopy) analysis data. The average waxy type amylose contents was 8.7% in landrace, variety and weed type, whereas 10.3% in breeding line. In common rice, the average amylose contents was 22.3% for landrace, 22.7% for variety, 23.6% for weed type and 24.2% for breeding line. Waxy type resources comprised of 5% of the total germplasm collections, whereas low, intermediate and high amylose content resources share 5.5%, 20.5% and 69.0% of total germplasm collections, respectively. The average percent of protein contents was 8.2 for landrace, 8.0 for variety, and 7.9 for weed type and breeding line. The average Variability Index Value was 0.62 in waxy rice, 0.80 in common rice, and 0.51 in protein contents. The accession ratio in arbitrary ranges of landrace was 0.45 in amylose contents ranging from 6.4 to 8.7%, and 0.26 in protein ranging from 7.3 to 8.2%. In the variety, it was 0.32 in amylose ranging from 20.1 to 22.7%, and 0.51 in protein ranging from 6.1 to 8.3%. And also, weed type was 0.67 in amylose ranging from 6.6 to 9.7%, and 0.33 in protein ranging from 7.0 to 7.9%, whereas, in breeding line it was 0.47 in amylose ranging from 10.0 to 12.0%, and 0.26 in protein ranging from 7.0 to 7.9%. These results could be helpful to build database programming system for germplasm management.

• Geotechnical Engineering
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• v.14 no.4
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• pp.177-204
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• 1998

In the present study, a method of quasi-three dimensional stability analysis is proposed for a systematic design of the GRS-RW(Geosynthetic-Reinforced Soil Retaining Wall) system based on the postulated three dimensional failure wedge. The proposed method could be applied to the analysis of the stability of both the straight-line and cove-shaped are. As with skew reinforcements. Maximum earth thrust expected to act on the rigid face wall is assumed to distribute along the depth, and wall displacements are predicted based on both the assumed compaction-induced earth pressures and one dimensional finite element method of analysis. For a verification of the procedure proposed in the present study, the predicted wall displacements are compared with chose obtained from the RMC tests in Canada and the FHWA tests in U.S.A. In these comparisons the wall displacements estimated by the methods of Christopher et at. and Chew & Mitchell are also included for further verification. Also, the predicted wall displacements for the convex-shaped zone reinforced with skew reinforcements are compared with those by $FLAC_{3D}$ program analyses. The assumed compaction-induced earth pressures evaluated on the basic of the proposed method of analysis are further compared with the measurements by the FHWA best wall. A parametric stduy is finally performed to investigate the effects of various design parameters for the stability of the GRS-RW system

• The Korean Journal of Nuclear Medicine Technology
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• v.16 no.2
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• pp.156-161
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• 2012

Purpose : IRMA method for the experimental reagents, as set out in the manual settings for doing and reporting the test results should, in principle. But many of the reagents allows for the setting of the plot does not have a lot of information. Depending on the angle setting of the table make a difference in test results and what settings are best suited to investigate. Materials and Methods : The hospital has Boramae DREAM10, Cobra, SR-300's using the Immunotech TSH reagents have been compared with the measurements. Since then there using EXCEL calculation of the equipment compared with the measurement. Results : In the comparison between each piece of equipment 1.SR-300: DREAM 10 y = 1.1376x - 0.046 (LOG-LOGIT), 2.SR-300: COBRA y = 1.0985x + 0.042 (LOG-LOG) 3.DREAM10: COBRA y = 0.965x + 0.0887 (LOG-LOG) was the result. In all charts the same X, Y axis, X values were good in general when you have. Linear-Logit value of Cobra Dream10 and Excel tends to match the calculated values provided. Conclusion : Is to guide the B / max, B / Total Logit Y axis of the chart is set to draw a look at the value equation when the X-axis LOG a high concentration are disadvantages rising urgently toward the slope. Linear-Logit plots close to the straight line has a curve. If you have a chart to guide on setting AS setting and therefore, set to guide the absence of information on the need to set up the experiment are thought to pass through.

• Journal of the Korean Magnetics Society
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• v.22 no.2
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• pp.49-57
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• 2012

From among the NDT (Non-Destructive Testing) methods, the MFL (Magnetic Flux Leakage) PIG (Pipeline Inspection Gauge) is especially suitable for testing pipelines because the pipeline has high magnetic permeability. MFL PIG showed high performance in detecting the metal loss and corrosions. However, MFL PIG is difficult to detect the crack which occured by exterior-interior pressure difference in pipelines and the shape of crack is very long and narrow. Therefore, the new PIG is needed to be researched and developed for detecting the cracks. The CMFL (Circumferential MF) PIG performs magnetic fields circumferentially and can maximize the magnetic flux leakage at the cracks. In this paper, CMFL PIG is designed and the distribution of the magnetic fields is analyzed by using 3 dimensional nonlinear finite element method (FEM). By Simulating and Measuring the magnetic leakage field, it is possible to detect of axial cracks in the pipeline.

• Geophysics and Geophysical Exploration
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• v.19 no.4
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• pp.212-219
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• 2016

The development of more advanced geophysical exploration techniques is necessary because the orebodies as yet discovered are increasingly changing in characteristics from shallow/high-grade to deep/low-grade. In this work, laboratory measurement of physical properties of rock samples and a field survey and interpretation of spectral induced polarization (SIP) have been conducted in a skarn metallic deposit, Gagok mine. The purpose of this study is that the applicability of SIP in the exploration of skarn metallic deposits is verified by the comprehensive interpretation between SIP characteristics of rocks obtained from the laboratory measurements and inverted survey results from the field data. In order to understand the SIP characteristics of each lithology, the data of eighty nine rock samples utilized in the previous studies were revaluated. The field survey was carried out using frequency of 0.25 Hz along a survey line designed for intersecting lithological boundaries and evaluating mineralized zones. The mineralized rocks were more conductive (low-resistivity) and capacitive (high-chargeability or strong-phase) than other rocks. Thus, SIP can be one of the very useful tools for the mineral exploration of the skarn deposits.