How about IRMA Curve

IRMA 법 Curve에 관하여

Purpose : IRMA method for the experimental reagents, as set out in the manual settings for doing and reporting the test results should, in principle. But many of the reagents allows for the setting of the plot does not have a lot of information. Depending on the angle setting of the table make a difference in test results and what settings are best suited to investigate. Materials and Methods : The hospital has Boramae DREAM10, Cobra, SR-300's using the Immunotech TSH reagents have been compared with the measurements. Since then there using EXCEL calculation of the equipment compared with the measurement. Results : In the comparison between each piece of equipment 1.SR-300: DREAM 10 y = 1.1376x - 0.046 (LOG-LOGIT), 2.SR-300: COBRA y = 1.0985x + 0.042 (LOG-LOG) 3.DREAM10: COBRA y = 0.965x + 0.0887 (LOG-LOG) was the result. In all charts the same X, Y axis, X values were good in general when you have. Linear-Logit value of Cobra Dream10 and Excel tends to match the calculated values provided. Conclusion : Is to guide the B / max, B / Total Logit Y axis of the chart is set to draw a look at the value equation when the X-axis LOG a high concentration are disadvantages rising urgently toward the slope. Linear-Logit plots close to the straight line has a curve. If you have a chart to guide on setting AS setting and therefore, set to guide the absence of information on the need to set up the experiment are thought to pass through.

IRMA법의 실험결과 측정 시 각 축의 설정과 곡선 유형에 따라서 값이 다르게 계산되어 나온다. 시약의 안내서에 있는 대로 설정을 하되 안내가 되어 있지 않은 경우에는 안내서의 도표를 참고하여 설정을 하거나. 어려가지 실험을 통해서 가장 최적화된 설정 값을 찾아내야 한다. 본 실험에서 Immunotech사의 TSH에서는 Linear-Logit 값이 가장 좋은값을 나타낸다고 사료된다. 작은 규모의 실험실에서 감마카운터가 고장 시 SR-300을 이용하여 CPM만 측정 후 EXCEL을 이용하여 결과값을 계산 후 보고해도 좋을 것같다.