• Asian-Australasian Journal of Animal Sciences
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• 제20권9호
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• pp.1397-1401
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• 2007

An in situ experiment was conducted to find out whether Tween 80 improves rice straw digestion through increased adhesion of major fibrolytic bacteria. Rice straw was sprayed with various levels of Tween 80 non-ionic surfactant or SDS ionic surfactant 24 h before incubation in the rumen of Holstein steers. Dry matter (DM) disappearance and adhesion of F. succinogenes, R. flavefaciens and R. albus on rice straw after in situ incubation were measured by real-time PCR. Application of Tween 80 increased DM disappearance, which was more noticeable at an application level of 1% compared to lower application levels. Application of SDS resulted in an opposite response in DM disappearance with highest reduction in DM disappearance at 1% level. In a subsequent in situ experiment, higher Tween 80 was applied to rice straw in an attempt to find the optimum application level. Tween 80 at 2.5% gave better DM disappearance than 1% with a similar result at 5%. Therefore, an adhesion study was carried out using rice straw treated with 2.5% Tween 80. Our results indicated that Tween 80 reduced adhesion of all three major rumen fibrolytic bacteria to rice straw. Present data clearly show that improved DM disappearance by Tween 80 is not due to increased bacterial adhesion onto substrates.

• 기술사
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• 제41권3호
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• pp.35-39
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• 2008

The most common soil contaminants are petroleum-based. Hydrocarbons from diesel fuel and gasoline are widespread problems, as are total petroleum hydrocarbon(TPH). There are two distinct classes of soil remediation: in-situ, or on-site, and ex-situ, or off- site. On-site cleanups are often preferred because they are cheaper. On the other hand, excavating a contaminated area and transporting it to a remote site before cleaning it can often be more complete. Ex-situ remediation also has the added bonus of taking the bulk of contaminants off-site before they can spread further. In addition, in-situ situations are limited because only the topside of the soil is accessible.

• 한국구조물진단유지관리공학회 논문집
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• 제13권1호통권53호
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• pp.152-160
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• 2009

강관추진공법은 비굴착공법으로써 중소규모 관로공사에 높은 시공성과 경제성을 가지고 있기 때문에 그 사용이 빈번해지고 있다. 그러나 강관추진공법의 추진과정은 원지반이 교란되고 느슨해지는 문제를 야기하고 있다. 이는 지표침하, 지반강도 저하 및 누수 등의 문제와 연결된다. 이에 본 연구는 강관추진과 동시에 그라우팅을 시행하는 그라우팅을 병행한 강관추진공법의 현장 적용성을 알아보고자 한다. 그 방법으로 그라우팅을 병행한 강관추진공법과 기존강관추진공법을 동일 지반에 시공하였다. 지표침하 계측, 현장밀도, GPR 물리탐사 그리고 대형전단실험의 결과는 그라우팅을 병행한 강관추진공법의 현장 적용성이 있음을 입증하였다.

• 한국지반공학회논문집
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• 제18권2호
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• pp.65-74
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• 2002

지중응력의 측정은 터널 및 지하구조물의 변위와 응력분포를 예측하는데 중요하다. 본 연구에서는 남부 한반도 지역의 지중응력 분포를 음력해방법과 수압파쇄법으로 측정된 새로운 380개의 자료를 통하여 재평가와 이를 고려한 삼차원 터널해석을 실시하였다. 지중응력분포의 결과에 의하면 변성암과 화강암지역의 수평응력분포는 퇴적암과 화산암의 분포에 비하여 불규칙한 경향을 보이고 있다. 화산암 지역에서는 수직응력에 대한 수평음력의 비(측압계수, K)는 150m 깊이 이상 심도에서 1보다 작다. 또한, 국내에서는 처음으로 측정된 지중응력에 대한 삼차원 방향과 크기를 동시에 그림으로 나타내었다. 삼차원 터널 해석에 의하면, 터널 주변의 변위에 대한 방향과 크기는 최대수평음력과 최소수평응력의 차이에 의하여 주로 영향을 받고 있다.

• 대한세포병리학회지
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• 제7권1호
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• pp.38-43
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• 1996

Epstein-Barr virus(EBV) is associated with a wide spectrum of benign and malignant disorders including leukoplakia, Hodgkln's lymphoma, central nervous system lymphoma, peripheral T cell lymphoma and nasopharyngeal undifferentiated carcinoma. There are several distinctive aspects of biology of the virus that are important in investigation of virus in clinical specimens. The abundant expression of the EBER mRNA transcripts makes possible the sensitive detection of latent expression in EBV-associated tumors. Although there has been a dramatic increased interest in the direct characterization of EBV in clinical specimens, there have been few studios about the effective and reliable positive controls in performing in situ hybridization technique for EBV, especially on paraffin-em bedded tissue. We applied Burkitts lymphoma ceil line as positive control in EBV in situ hydridization using Oncor Kit. The cell block of Burkitt lymphoma cell line(CCL85 EB-3) showed strong and specific positivity for EBER in situ in nuclei of EBV infected cells.

• 한국도로학회논문집
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• 제17권3호
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• pp.85-95
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• 2015

PURPOSES : Recently, bonded concrete overlay has been used as an alternative solution in concrete pavement rehabilitation since its material properties are similar to those of the existing concrete pavements. Deteriorated concrete pavements need rapid rehabilitation in order to prevent traffic jams on Korean expressways. Moreover, speedy and effective repair methods are required. Therefore, the use of bonded concrete overlay with ultra-rapid hardening cement has increased in an effort to reopen promptly the expressways in Korea. However, mobile mixer is required for ultra-rapid hardening cement concrete mixing in the construction site. The use of mobile mixer causes various disadvantages aforementioned such as limitation of the construction supply, open-air storage of mixing materials, increase in construction cost, and etc. In this study, therefore, hydration accelerator in-situ mixing on polymer modified concrete produced in concrete plant is attempted in order to avoid the disadvantages of existing bonded concrete overlay method using ultra-rapid hardening cement. METHODS : Bonded concrete overlay materials using ultra-rapid hardening cement should be meet all the requirements including structural characteristics, compatibility, durability for field application. Therefore, This study aimed to evaluate the application of hydration accelerator in-situ mixing on polymer modified concrete by evaluating structural characteristics, compatibility, durability and economic efficiency for bonded concrete overlay. RESULTS : Test results of structural characteristics showed that the compressive, flexural strength and bond strength were exceed 21MPa, 3.15MPa and 1.4MPa, respectively, which are the target strengths of four hours age for the purpose of prompt traffic reopening. In addition, tests of compatibility, such as drying shrinkage, coefficient of thermal expansion and modulus of elasticity, and durability (chloride ions penetration resistance, freezing-thawing resistance, scaling resistance, abrasion resistance and crack resistance), showed that the hydration accelerator in-situ mixing on polymer modified concrete were satisfied the required criteria. CONCLUSIONS : It was known that the hydration accelerator in-situ mixing on polymer modified concrete overlay method was applicable for bonded concrete overlay and was a good alternative method to substitute the existing bonded concrete overlay method since structural characteristics, compatibility, durability were satisfied the criteria and its economic efficiency was excellent compare to the existing bonded concrete overlay methods.

• 한국의류산업학회지
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• 제22권3호
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• pp.386-398
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• 2020

This study compared dip-coating and in situ polymerization methods for the development of nanofiber-based E-textile using polypyrrole. Nanofiber webs were fabricated by electrospinning an aqueous poly (vinyl alcohol) (PVA) solution. Subsequently, the PVA nanofiber web underwent thermal treatment to improve water resistance. Dip-coating and in situ polymerization methods were used to deposit polypyrrole on the surfaces of the nanofiber web. An FE-SEM analysis was also conducted to examine specimen surface characteristics along with EDS and FT-IR that analyzed the chemical bonding between polypyrrole and specimens. The line resistance and sheet resistance of the treated specimens were measured. Finally, an electrocardiogram (ECG) was measured with textile sensors made of the polypyrrole-deposited PVA nanofiber webs. The polypyrrole-deposited PVA nanofiber webs fabricated by dip-coating dissolved in the dip-coating solution and indicated damage to the nanofibers. However, in the case of in situ polymerization, polypyrrole nanoparticles were deposited on the surface and inter-web structure of the PVA nanofiber web. The resistance measurements indicated that polypyrrole-deposited PVA nanofiber webs fabricated by in situ polymerization with an average sheet resistance of 5.3 k(Ω/□). Polypyrrole-deposited PVA nanofiber webs fabricated by dip-coating showed an average sheet resistance of 57.3 k(Ω/□). Polypyrrole-deposited PVA nanofibers fabricated by in situ polymerization showed a lower line and sheet resistance; in addition, they detected the electrical activity of the heart during ECG measurements. The electrodes made from polypyrrole-deposited PVA nanofiber webs by in situ polymerization showed the best performance for sensing ECG signals among the evaluated specimens.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2000년도 말뚝기초 학술발표회
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• pp.65-84
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• 2000

Europe and US which have more restrictive regulations than Korea about the noise and vibration during construction are using Auger-cast Pile to reduce the problem relating with noise and vibration. However Auger-cast Pile has problems like difficult quality control and low bearing capacity. In Europe, Displacement in-situ concrete Pile has been used to sove that problems since 1990s, and Korea has performed the test construction in 1997 and it has been used as the real structural foundation since 1998. Test and real construction results verified that the allowable capacity of the pile(diameter = 410mm) is between 70 and 100ton. Though De Beer & Van Imps design method utilizing CPT result is used to calculate the bearing capacity of the Displacement in-situ Pile, Korea is dependant upon the SPT as the sounding test, so design method utilizing SPT result is necessary to promote the application of the pile. To find out reasonable design method using SPT result, rearing capacity of the pile constructed in sand and clay in Korea was calculated using Meyerhof, SPT-CPT translation method, Nordlund, Douglas and DM-7 method, and the calculation results were compared to the load test result. Analysis result shows that SPT-CPT translation method is more reliable than others and economical design can be possible because it considers efficiently the friction capacity of Displacement in-situ Pile.

• 한국물환경학회지
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• 제29권5호
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• pp.691-702
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• 2013

This review paper summarizes the theory, application, and potential drawbacks of diffusive gradient in thin film (DGT) probe which is a widely used in-situ passive sampling technique for monitoring inorganic contaminants in aquatic environments. The DGT probe employs a series of layers including a filter membrane, a diffusive hydrogel, and an ionic exchange resin gel in a plastic unit. The filter side is exposed to an aquatic environment after which dissolved inorganic contaminants, such as heavy metals and nuclides, diffuse through the hydrogel and are accumulated in the resin gel. After retrieval, the contaminants in the resin gel are extracted by strong acid or base and the concentrations are determined by analytical instruments. Then aqueous concentrations of the inorganic contaminants can be estimated from a mathematical equation. The DGT has also been used to monitor nutrients, such as ${PO_4}^{3-}$, in lakes, streams, and estuaries, which might be helpful in assessing eutrophic potential in aquatic environments. DGT is a robust in-situ passive sampling techniques for investigating bioavailability, toxicity, and speciation of inorganic contaminants in aquatic environments, and can be an effective monitoring tool for risk assessment.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2003년도 International Symposium
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• pp.89-110
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• 2003

최근 현장(In situ)에서의 토양 및 지하수 오염 정화는 오염물질을 현장에서 추출해 지상에서 처리하는 것이 아니라, 직접 현장에서 미생물 혹은 화학물질과 오염물질의 혼합을 통한 분해기 작을 이용해, 오염물질을 제거하는 과정을 의미한다. 이러한 현장오염정화 기법 중 하나가 Figure 1에 도시한 수직흐름처리정(Horizontal Flow Treatment Wells, HFTWs)으로 2개의 정(well) 중간지점에 반응물질을 설치한 후 각각의 정에서 상향 및 하향흐름을 발생시켜 주위의 지하수를 순환시키면서 정화하는 기법이다.