• KSBB Journal
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• v.15 no.3
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• pp.247-253
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• 2000

This study was aimed to enhance polycyclic aromatic hydrocarbon(PAHS) biodegradation rate by repeated-batch treatment using immobilized cells of Phanerochaete chrysosporium. In the repeated-batch operations with 30 mg/L of pyrene the maximum degradation rate was 6.58 mg/L day. As the number of batches increased the concentration of immobilized cells significantly decreased and the degradation rate and specific acitivity gradually increased to a maximum value and then decreased. To have PAH degradation activity and cell mass recovered one batch of cultivation using the growth medium instead of the PAH-degrading medium was carried in the course of repeated-batch operations. This maximum degradation rates of pyrene and anthracene were 4.29 and 4.46 mg/L$.$day respectively. Overall the rate of PAH degradation could be enhanced 2.5-30 folds by using immobilized cells compared to the case of using suspended cells.

• Biotechnology and Bioprocess Engineering:BBE
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• v.1 no.1
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• pp.26-31
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• 1996

Since biosynthesis of lignin peroxidase from Phanerochaete chrysosporium was known to be sensitive to shear, it is interesting to understand the effects of the shear sensitivity for the overproduction of lignin peroxidase. In stirred-tank fermentor, the shear-sensitivity in lignin peroxidase biosynthesis was quantified by using Kolmogorov length scale. It was found that agitation at 80$\mu$m Kolmogorov length scale is advantageous for the production of lignin peroxidase from P. chrysosporium. To overcome the shear sensitivity in lignin peroxidase biosynthesis caused by the agitation,P. chrysosporium was immobilized on various solid carriers. The nylon-immobilized P. chrysosporium was chosen in the present study as a way to overcome the shear sensitivity at the ranges of above 50$\mu$m Kolmogorov length scale. The adhesion force between immobilized cell and carrier can be predicted by thermodynamic approach and used as a criteria to select an adequate carrier materials for immobilization.

• Textile Coloration and Finishing
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• v.12 no.1
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• pp.32-43
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• 2000

Batch culture system and continuous culture systems were used to investigate the removal of disperse dye using white rot fungi. White rot fungi used in the study were Coriolus hirsutus IFO 4917, Lenzites betulina IFO 6266, Coriolus versicolor IFO 30340 and Phanerochaete chrysosporium IFO 31249. The results of the batch culture experiment showed that white rot fungi used in this study had excellent dye removal abilities. Phnerochete chrysosporium IFO 31249 was especially effective on the removal of disperse dyes. And continuous treatment of disperse red 60 was studied under two type of reactor using Phanerochaete chrysosporium IFO 31249. The removal efficiency of disperse red 60 for immobilized Phanerochaete chrysosporium IFO 31249 in continuous reactor with vertical matrix was increased 1.3 fold in $1.4\;hr^{-1}$ dilution rate when compared with continuous reactor without vertical matrix.

• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.163-163
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• 2005

• KSBB Journal
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• v.17 no.1
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• pp.14-19
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• 2002

The objective of this study is to investigate optimum condition for lignin peroxidase production by white rot fungi Phanerochaete chysosporium IFO 31249 immobilized in a rotating bioreactor. The maximum lignin peroxidase activity of batch culture in rotating bioreactor was 300 U/L. The optimum rotating speed and packing ratio of support for lignin peroxidase production in a rotating bioreactor were 1 rpm and 20%, respectively. The optimum concentration of $MnSO_4$$\cdot$$H_2O$ for manganese-dependent peroxidase production in a rotating bioreactor was 50 ppm. The sufficient supply of oxygen was the most important factor to achieve maximum lignin peroxidase production. It was possible to produce lignin peroxidase (LiP) and manganese-dependent peroxidase (MnP) for at least 3 times successive repeated-batch cultures, respectively.