Biodegradation of PAHs (Polycyclic Aromatic Hydrocarbon) Using Immobilized Cells of Phanerochaete chrysosporium

고정화 Phanerochaete chrysosporium을 이용한 다환 방향족 화합물의 분해

  • 서윤수 (한국과학개술원 생물공정연구센터 및 화학공학과) ;
  • 류원률 (영남대학교 공과대학 응용화학부) ;
  • 김창준 (한국과학기술원 생물공전연구센터 및 화학공학과) ;
  • 장용근 ;
  • 조무환 (영남대학교 공과대학 응용화학부)
  • Published : 2000.06.01

Abstract

This study was aimed to enhance polycyclic aromatic hydrocarbon(PAHS) biodegradation rate by repeated-batch treatment using immobilized cells of Phanerochaete chrysosporium. In the repeated-batch operations with 30 mg/L of pyrene the maximum degradation rate was 6.58 mg/L day. As the number of batches increased the concentration of immobilized cells significantly decreased and the degradation rate and specific acitivity gradually increased to a maximum value and then decreased. To have PAH degradation activity and cell mass recovered one batch of cultivation using the growth medium instead of the PAH-degrading medium was carried in the course of repeated-batch operations. This maximum degradation rates of pyrene and anthracene were 4.29 and 4.46 mg/L$.$day respectively. Overall the rate of PAH degradation could be enhanced 2.5-30 folds by using immobilized cells compared to the case of using suspended cells.

Keywords

References

  1. Current Opinion in Biotechnology v.8 Polycyclic aromatic hydrocarbon bioremediation design Harayama, S
  2. Current Opinion in Biotechnology v.4 Biodegradation of polycyclic aromatic hydrocarbons Cerniglia, C. E
  3. Appl. Environ. Microbiol v.58 Biodegradation of polycyclic aromatic hydrocarbons by new isolates of white rot fungi Field, J. A., E. D. Jong, G. F. Costa;J. A. M. de Bont
  4. Appl. Environ. Microbiol v.61 One-lectron oxidation in the degradation of creosote polyclic aromatic hydrocarbons by phanerochaete chrysosporium Bogan, B. W;R. T. Lamar
  5. Appl. Microbiol. Biotechnol v.44 Oxidation of anthracenes in water/solvent mixtures by the whiterot fungus, Bjerkandera sp. strain BOS55 Field, J. A., F. Boelama, Baten;W. H. Rulkens
  6. PAHs and relaed compounds(2nd ed) Neilson, A. H
  7. Curr. Opin. Biotechnol v.6 The Potential for white-rot fungi in the treatment of pollutants Reddy, C. A
  8. Appl. Environ. Microbiol v.60 Nitrogen regulation of lignin peroxidase gene transcription Dan, L., M. Alic;M. H. Gold
  9. Ph. D. Dissertation Development of two stage continous fermentation process for cyclosporium A production by using immobilized fungal cell Lee, T. H
  10. Meth. Enzymol v.161 Lignin peroxidase of Phanrochatet chrysosporium Tien, M;T. K. Kirk
  11. Biotechnol. Bioeng v.25 Confining mycelial growth to porous microbeads : a novel technique to alter the morphology of non-newtonian mycelial culture Gbewonyo, K;D. I. C. Wang