• Korean Journal of Veterinary Pathology
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• v.5 no.2
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• pp.67-70
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• 2001

Spontaneous subcutaneous cysts were detected from three dogs, being a 4-year-old male shih tzu, an 11-month-old female britany spaniel and a 9-month-old male mongrel. All the cysts were occurred around the joints (one elbow's and two shoulderes', respectively). After surgical removal, the lesions did not recur for 6 months follow-up. Neither cyst communicated with the joint cavity. Grossly, the subcutaneous ovoid cysts had moderate to abundant mucinous fluid. Histologically, the cyst wall consisted of inner myxomatous and outer immature connective tissue. Some parts of the cyst wall had various stages of myxoid metaplasia of collagen tissue leading to new cyst formation. The true lining cells of the inner cyst wall were not observed in all the cysts. From these results, these cases were diagnosed as subcutaneous ganglion cysts. This is the first report of canine subcutaneous ganglion cysts in Korea.

• Animal cells and systems
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• v.7 no.2
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• pp.105-109
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• 2003

Morphological characteristics of nymphal instars are described for a Korean wood-feeding cockroach, Cryptocercus kyebangensis. Eleven instars, including adults, were tentatively identified. Female adults had apicolateral emargination and a truncated apical median prominence in the seventh sternite, and female nymphs of the other instars except for the last had a narrowly rounded apical median prominence in the seventh sternite. In the last instar, the seventh sternite was partially desclerotized and somewhat shriveled at the start of the emarginated area. In contrast, males showed no emargination in adults, and had a rounded broad apical median in this area of all instars. In the ninth sternite, female nymphs had a medium notch on the caudal margin and styli were separated before reaching adulthood, whereas no such notch was observed in male nymphs. The styli remained prominent in the ninth sternite of male adults.

• IMMUNE NETWORK
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• v.14 no.6
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• pp.328-332
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• 2014

Dexamethasone (Dex) was shown to inhibit the differentiation, maturation, and antigen-presenting function of dendritic cells (DC) when added during DC generation or maturation stages. Here, we examined the direct effects of Dex on MHC-restricted antigen processing. Macrophages were incubated with microencapsulated ovalbumin (OVA) in the presence of different concentrations of Dex for 2 h, and the efficacy of OVA peptide presentation was evaluated using OVA-specific CD8 and CD4 T cells. Dex inhibited both class I- and class II-restricted presentation of OVA to T cells; this inhibitory effect on antigen presentation was much more potent in immature macrophages than in mature macrophages. The presentation of the exogenously added OVA peptide SIINFEKL was not blocked by Dex. In addition, short-term treatment of macrophages with Dex had no discernible effects on the phagocytic activity, total expression levels of MHC molecules or co-stimulatory molecules. These results demonstrate that Dex inhibits intracellular processing events of phagocytosed antigens in macrophages.

• The Korean Journal of Ecology
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• v.27 no.6 s.122
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• pp.369-373
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• 2004

The water strider, Gerris paludum insularis, was reared in a growth cabinet at two constant temperatures and ${\approx}20^{\circ}C$ under a 16:8 (L:D) photoperiod. Differences in head width among three temperatures of ${\approx}20^{\circ}C,\;25^{\circ}C\;and\;30^{\circ}C$ in G. paludum insularis, were compared. Temperature affected growth of head width of G. paludum insularis. There were highly significant differences among head width for three temperature regimes and the immature stages. It was found that head width is greater in $30^{\circ}C$ than $25^{\circ}C\;and\;{\approx}20^{\circ}C$. The growth quantity of the head width in ${\approx}20^{\circ}C$ shows the slowest increase, and $25^{\circ}C\;and\;30^{\circ}C$ were gradually increased in that order. The maximum ratio of growth quantity by each stage is in the first instar in all regimes. The growth rates of all regimes show an increase pattern.

• Clinical and Experimental Reproductive Medicine
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• v.18 no.1
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• pp.55-61
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• 1991

In order to increase the pregnancy rate by means of cryopreservation of the excess oocytes in IVF-ET program, the survival rate of the frozen-thawed oocytes of mouse was examined according to the stages of maturation, cryoprotectants and their treatment. The results were summarized as follows. First, during the continuous treatment with cryoprotectant media, the survival rate of oocytes was higher in DMSO than in PROH, and higher at low temperature($4^{\circ}C$) than at room temperature($25^{\circ}C$). Second, as regard with the maturation of immature(GV-intact) oocytes after treatment with cryoprotectant media, the rate of maturation in DMSO-treated group(52%) was higher than in PROH-treated group(35%). Third, according to the treatment of cryoprotectant media, the survival rate of frozen-thawed oocytes in DMSO-treated group (45%) was higher than in PROH-treated group(29%), and that of oocytes in DMSO 4-step treated group was higher than any other groups. Finally, in the post-thaw oocytes frozen at various stage of maturation, the survival rate of immature oocytes with GV was the highest in all groups. These results suggest that in the cryopreservation of mouse oocytes, DMSO was better than PROH as cryoprotectant, in treatment of cryprotectant the multi-step treatment was better than single-step, and the post-thaw survival rate of oocytes was closely related to the maturity of oocytes. It is assumed that the highest survival rate of mouse oocytes with GV is due to the stability of the structures in nucleus and intracelluar organelles, and of physiological function.

• Animal cells and systems
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• v.13 no.4
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• pp.439-445
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• 2009

Cytochrome P450 aromatase (P450arom) catalyzes the conversion of androgens to estrogens and plays an important role in reproduction and development in vertebrates. We investigated the expression patterns of ovarian P450arom (P450aromA) and brain P450arom (P450aromB) mRNA during sex change in black porgy. Maturity was divided into seven stages from male to female (immature testis, mature testis, testicular portion of mostly testis, ovarian portion of mostly testis, testicular portion of mostly ovary, ovarian portion of mostly ovary, and mature ovary). P450aromA expression was significantly higher in the ovarian portion of mostly-ovarian stage fish, and P450aromB expression was highest in the brain of black porgy with mostly-ovarian gonads. Histology showed that testicular tissues were disintegrated with the development of ovarian tissue associated with an increase in the expression of the two P450arom mRNAs during sex change. Interestingly, among various tissues, P450aromA was only expressed in the ovary, and P450aromB was only expressed in the brain. To understand the role of gonadotropin-releasing hormone (GnRH) and estradiol ($E_2$), we injected exogenous hormone (GnRH analogue [GnRHa] and $E_2$) into immature black porgy. In the GnRHa group, expression of the two P450arom genes decreased 12 h after injection, and expression of the two P450arom genes were significantly higher at 6 dafter $E_2$ injection. These results provide useful baseline knowledge on the mechanism of natural sex change in black porgy.

• Clinical and Experimental Reproductive Medicine
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• v.26 no.3
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• pp.345-353
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• 1999

In the studies on the hatching mechanisms in mammals, many investigators focused on the embryonic intrinsic factor(s) in in vitro culture, but the uterine environment as the extrinsic factor(s) is thought to play an important role in hatching mechanism. Therefore, to evaluate the effect of uterine environment on the hatching event in vivo, the immature(GV) and ovulated(MII) oocytes, and the late 2-cell embryos of mouse were transferred to pseudopregnant foster mother's uterus during peri-implantation period. So it was verified whether there would happen hatching by only uterine environment independently on embryonic stage. The ultrastructural changes of the zona surface of transferred group were compared with those 01 in vivo and vitro group by SEM. 36 hrs after transfer, the immature and ovulated oocytes almost degenerated, and the late 2-cell embryos developed to various embryonic stages. However, the embryos which didn't develop to blastula stage did not hatch. The ultrastructural network of ZP in transferred group seemed to be smoothed uniformly, which was different from in vitro group. In conclusion, it is suggested that the uterine environment during peri-implantation period enhances the embryo hatching by provoking the structural change of ZP.

• Korean Journal of Plant Tissue Culture
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• v.22 no.4
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• pp.189-194
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• 1995

Calli were successfully induced from immature embryos of Citrus junos SIEB. cultured on 1/2 MS medium supplemented with 40.4 BA. Plant were regenerated from immature embryo derived callus on MS medium with 5 $\mu$M BA. The calli were morphologically characterized by two types: one was whitish and the other was yellowish. After 16 weeks of culture, shoots and root were formed on calli. Plantlets were transplanted to soil and successfully grown to a whole plant Also, the arrangement of the cells showed many differences according to developmental stages of callus and organogenesis. The small cells were compact in callus cultured for 6 weeks and the extended cells which divided actively appeared in it after 8 weeks of culture. The globular protrusion of compacted cells occurred in callus after 10 weeks of culture, and the neighboring cells were liquefied. Oil sac surrounded by the liquefied cell was observed in the leaf and was formed by rupture of liquefied cells.

• Current Research on Agriculture and Life Sciences
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• v.16
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• pp.31-36
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• 1998

In order to investigate the effect of cytokinins on the cotyledonary variability of somatic embryos in Heracleum moellendorffii Hance., somatic embryos were induced from the immature zygotic embryo on MS medium containing 1.0mg/l 2, 4-D, and then transferred to 2,4-D-free and cytokinin-added medium for somatic embryogenesis after 4weeks culture. Polycotyledonary embryos were formed most abundantly(84.9%) on the 0.2mg/l BA medium as compared with the 0.2mg/l 2ip(42.6%) and kinetin(32.9%) media, and it was proportional to BA concentrations(0.01~0.1mg/l). The rate of polycotyledonary embryo formation increased proportional to the period of BA treatment and also increased more at the globular stage than at the heart, torpedo-shaped and cotyledonary stages.

• Korean Journal of Food Science and Technology
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• v.54 no.3
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• pp.251-256
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• 2022

This study was conducted to determine whether Diospyros lotus L. fruit could be used as a natural functional food ingredient and whether its use should be increased. D. lotus fruit powder was found to have high acidity in the immature IDL stage and significantly higher sugar content in the mature MDL stage. The levels of crude fat, selenium, and tannins decreased significantly from the IDL stage to the MDL stage in D. lotus fruit powder. However, the levels of magnesium and calcium were significantly greater in the MDL stage than in the IDL stage (p<0.05). The total phenol content was significantly higher in the IDL stage, but the DPPH radical scavenging activity significantly increased in the MDL stage (p<0.05). In conclusion, the fruits of D. lotus. have high nutritional quality in both the immature and mature growth stages. Thus, it is a good candidate for use as a functional food, and its use should be increased because of its high nutritional qualities.