• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.2
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• pp.245-249
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• 1995

The number of immature spikes of barley (Hordeum vulgare L.) and wheat (Triticum aestivum L) were studied to determine if there could be existed the significant difference among 5 varieties of barley and wheat each. Heritability of 6 characters including the number of immature spikes have also calculated. Correlation between the immature spikes and 5 other characters were also investigated. There were significant difference among 5 varieties of barley and wheat in the number of immature spikes. Heritability estimated were very high in case of number of immature spikes of barley and that of wheat was high. There were highly significant correlation between the number of immature spikes of barley and number of internode and significant correlation between the number of immature spikes and flag leaf. However, in wheat no correlations were found among the 5 characters in wheat and between the number of immature spikes and number of internode were negative.

• Korean Journal of Food Science and Technology
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• v.26 no.3
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• pp.336-342
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• 1994

Changes in physicochemical characteristics were investigated for immature barley kernels roasted at $160{\sim}220^{\circ}C$ for $1{\sim}12$ min. Only small differences in chemical constituents including starch, protein, fat, ash, total dietary fiber, and ${\beta}-glucan were observed between immature and mature barley kernels. The amounts of 75% ethanol-soluble sugars and amino acids present in immature barley kernels were considerably higher than those in mature kernels, and gradually decreased in the process of roasting. Of free sugars, sucrose, raffinose, glucodifructose($GF_{2}$) and maltose were reduced by roasting. Glucose and fructose, simple reducing sugars, decreased at the early stage of roasting, followed by a slight increase at the later stage. Starch and nitrogen contents decreased slowly, while TDF(total dietary fiber) had a tendency to increase slightly. Stacking volume of immature barley kernels increased markedly, especially at the higher temperatures. L value of immature barley decreased throughout roasting, and a, b values increased at the early stage of roasting but b value decreased with continued roasting. The degree of roasting was strongly affected by the roasting temperature. Darkness of immature barley kernel, depending on the degree of roasting, was highly associated with concentrations of brown pigments extracted from roasted immature barley kernels.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.52 no.1
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• pp.36-44
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• 2007

In order to evaluate the utilization of immature green barley kernels as food processing material, these experiments were carried out to analyze free sugars, free amino acids, mineral contents and color of two naked barley varieties according to their heading dates. Contents such as moisture, protein, fat, ash, and free sugars were gradually decreased according to the days after heading of the nonwaxy naked barley 'Saessalbori' and waxy naked barley 'Hinchalssalbori', while crude fiber and starch were gradually increased. Contents like maltose and maltotriose in immature green kernels were high, but free amino acids showed a tendency to decrease, among which the alanine content was the highest. As barley matured, beta-glucan content, redness and yellowness increased, while lightness decreased. Considering chemical composition and color, the immature green cereals were produced through blanching the spikes harvested before the yellow ripe stage around $26{\sim}27$ days after heading. The optimum duration of harvest was very limited to produce green whole grains. The harvested green barley cereals is able to eat raw barley or cook it as food processing material.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.6
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• pp.571-576
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• 1994

This study was conducted to determine the effects of embryo age, growth regulators, low temperature treatment on regeneration, plant growth, and heading in immature embryo culture of Barley. Shoot and root development was more in older than in younger excised embryos, and more in a medium without kinetin than in one with kinetin. The rate of survival was higher on medium without growth regulators and lower on medium with kinetin 5 mg/L because of retarded plant growth. Embryo age and media did not completely replace low temperature treatment. Twenty- and 14 day-old embryos responded by flowering after 4 weeks of vernalization. Embryo culture at 14- and 20 days after anthesis coupled with 4-week cold treatment shortens generation time of barley. When 20 day immature embryos of barley (cv. Olbori) was treated low temperature treatment for 4 weeks, the rate of heading was better than the other treatment.

• Applied Biological Chemistry
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• v.36 no.6
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• pp.456-462
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• 1993

Parboiling, a popular technology used to protect rice from nutrient loss during milling and to increase the shelf-life of rice, was applied to barley kernels, and its effect on nutrient retention and chemical composition was evaluated. Before 36 days after ear emergence, barley kernels showed water content higher than 40%, and parboiling without presoaking resulted in at least 43% of gelatinization degree. This implies that soaking, an important step of parboiling, is dispensable for barley at milky stage. Parboiling did make little change in the appearance of the kernel after 31 days from ear emergence. Nonreducing sugars such as sucrose and raffinose remained unchanged while reducing sugars of barley was decreased by parboiling, with exception that maltose increased. Pearling led to decrease in crude protein, fat, fiber and minerals of barley sampled and parboiled on 36th day from ear emergence. Free sugars in the parboiled barley also was reduced with increasing pearling rate. Vitamin $B_1$ content of the parboiled barley was $260\;{\mu}g\;per\;100\;g$ as dry basis at 50% pearling rate, compared to $36\;{\mu}g$ for raw barley at same pearling rate. Thus parboiling appeared to be very effective in the retention of vitamin $B_1$ during pearling.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.3
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• pp.308-313
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• 1995

In relation to shortening generation by using embryo culture of barley immature seed, it is important to find out profitable embryo age for embryo culture and to understand the relationship among embryo and other characters. Two varieties(Olbori and Dusan #12) were cultivated under two growth conditions(15/10 and 25/15$^{\circ}C$). The embryos were aseptically excised when immature seeds were collected 9, 13, 17, 21, 25 and 29 days after heading and cultured on $B_5$ medium. On the 21st day after heading, the length of embryos from top or middle part of spike was longer than that from bottom part. Embryos from bottom part under low temperature condition had the shortest length. Shoot length, root length and root number after embryo culture were little difference among three parts of spike under high temperature condition. Under low temperature, seedlings from bottom part of spike were inferior to those from top or middle part. Length of 29-day-old embryos under low temperature condition was similar to that of 17-day-old ones under high. Under high temperature condition, the length of 17-day-old embryo had positive correlation with kernel width, shoot length, root length and root number, but that of 21-day-old one didn't have. Seventeen-day-old embryos obtained from 25/15$^{\circ}C$ growth condition seem to be efficient to shortening generation by using embryo culture.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.10a
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• pp.167-167
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• 2003

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.3
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• pp.257-263
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• 1992

Immature embryos were tested for investigating the effect of cold treatment duration and GA$_3$ concentration on the vernalization response in barley. Immature embryos were cultured on B$_{5}$ medium with GA$_3$ for 5 days, and were transplanted under 20/15$^{\circ}C$ temperature after cold treatment. Germination rate and shoot length were increased more in 20-day-old embryos than those in 13-day-old ones but germination day was decreased. Addition of 1 or 10 ppm of GA$_3$ to B5 medium was effective on the growth of immature embryos. The higher GA$_3$ concentration was, the shorter time from embryo culture to flag leaf emergence and heading was. The earlist flag leaf emergence and heading were showed on treatment of 1 or 10 ppm of GA$_3$, but GA$_3$ did not completely replace vernalization. The days to flag leaf emergence of immature embryo-cultured barley with cold treatment for two and three weeks was shortened by 3 and 18 days at 1 ppm GA$_3$, and 16 and 20 days at 10 ppm GA$_3$, respectively, when compared to 0 ppm GA$_3$ culture with cold treatment for three weeks. It was at 10 ppm GA$_3$ with 3 weeks cold treatment that showed the lowest culm length, spike length and number of grain per spike. GA$_3$ concentration was not correlated significantly with each character in 1 week cold treatment, but was significant with each character in 2 weeks. In 3 weeks cold treatment, it was except for days to heading. Correlation between cold treatment duration and culm length was negative in 0 ppm GA$_3$. In 1 or 10 ppm of GA$_3$, all characters had highly negative correlation with cold treatment duration.n.

• Applied Biological Chemistry
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• v.36 no.6
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• pp.449-455
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• 1993

Some chemical properties were investigated for barley kernels at different growth stages. Crude fat, crude protein, starch, alcohol-insoluble solids(AIS), and ${\beta}-glucan$ increased until $31{\sim}36\;days$ from ear emergence and thereafter remained relatively constant, whereas ash and crude fiber contents were gradually decreased through the whole period of maturation. As barley kernels became mature, their water contents kept decreasing, and the content of milky stage barley kernel at $31{\sim}36th\;days$ from ear emergence ranged from $40{\sim}50%$. Free sugars including glucose, fructose, sucrose, raffinose, maltose, and kestose were identified in the growing kernels. Glucose, sucrose and kestose decreased with maturation while raffinose slightly increased. Barley kernels on the 43rd day from ear emergence contained 0.62% sucrose, 0.46% raffinose, 0.33% kestose, 0.19% glucose, 0.17% fructose, 0.04% maltose. Analysis of minerals for barley kernels at different growth stages showed that the growing kernels contained K, P, Mg, Ca, Na, Fe, Zn, Mn, Cu in decreasing order. Vitamin $B_1$ content tended to increase with kernel growth, showing maximum value of $350\;{\mu}g\;per\;100\;g$ dry weight on 36th day from ear emergence.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.28 no.4
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• pp.445-450
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• 1983

To investigate the effect of harvest time after heading, storage temperature and duration on the dormancy and germination in barley cultivars, the kernels were collected at 5 day intervals from 20 days after heading until 35 days. The kernels were dried at 2$0^{\circ}C$ for 7 days and storaged in freezing chamber (-15$^{\circ}C$). The kernels germinated better at 15$^{\circ}C$ than at $25^{\circ}C$, and 1% $H_2O$$_2$ and 3 ppm GA$_3$ treatments gave much higher germination percentage than non-treatment; $H_2O$$_2$ and GA$_3$ treatments were good for promoting germination of immature harvested kernels. Germination rate index were significantly correlated with germination percentage under $25^{\circ}C$ and 15$^{\circ}C$ temperature conditions. Hangmi, Jogangbori and Muanbori had deep or slight dormancy at the different harvesting time, especially Dusan #8 had the deepest dormancy. The dormancy broke more rapidly under 3$0^{\circ}C$ than 2$0^{\circ}C$ storage conditions, so high temperature accelerated dormancy breaking. Those results showed that dormancy was various with the cultivars, harvesting time, storage temperature and storage duration.