• The Journal of Korean Society of Virology
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• v.28 no.3
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• pp.225-232
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• 1998

We have expressed GFP in Sf9 and Bm5 cells or Bombyx mori larvae by using Ac-Bm hybrid virus capable of replicating in both Bm5 and Sf9 cells. Genomic DNA of Ac-Bm hybrid virus expressing ${\beta}$-galactosidase was cotransfected with baculovirus transfer vector containing GFP gene, pBacPAK-GFP in Sf9 cells. The Ac-Bm hybrid virus harboring GFP was named as Ac-Bm hybrid virus-GFP. The Ac-Bm hybrid virus-GFP-infected insect cells were easily selected by detecting the emission of GFP from each well of cell culture dish on the UV illuminator. GFP produced by Ac-Bm hybrid virus-GFP in Sf9 and Bm5 cells or B. mori larvae was confirmed by SDS-PAGE and Western blot analysis using GFP antibody. In addition, B. mori larvae infected with Ac-Bm hybrid virus-GFP was apparently appeared fluorescence from the whole body at S days postinoculation. The fluorescence of GFP from the hemolymph and fat body of B. mori larvae infected with Ac-Bm hybrid virus-GFP was also observed by fluorescence microscope. In conclusion, our results demonstrated that in baculovirus expression vector system, use of Ac-Bm hybrid virus have an additional advantage of expanded host range for producing recombinant proteins.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.18 no.11
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• pp.2786-2791
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• 2014

This paper describes the smart illuminating system based on the obstacle environmental presetting to improve the user convenience to easy lighting and energy conservation. Obstacle environment has trouble controlling the illuminating equipment using manual buttons in certain circumstances, which requires a smart remote controller. The smart remote controller is operated by the smart phone, motion sensor and timer to turn on and off the lamps. The event sensor module transmits the signals of the event occurrence to equipment on the remote place when smart phone and motion sensor detect an event, and the illuminator received the event turn on or off the lamp. The system results in energy saving by simple on/off control and manipulating the operating time with controlling the illuminating system preset by user's obstacle or preference circumstances. The proposed system implementation is experimented to figure out the energy saving about13.5w/h and the optimized convenience control.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.62 no.2
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• pp.257-263
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• 2013

In case of image observation equipments, CCTV for short distance visual field is usually installed and operated mostly as the means of crime-prevention. However, the extensive demand for monitoring problems in case of the increase in intelligent crimes and disasters has led to the necessity of the development of long-distance observation equipments embedded with Night View functions. In case of the Night View equipments, the relevant market is set up to be focused mostly on Thermal Observation Device(hereinafter, TOD), but some shortcomings such as the limitation of image visibility and excessive maintenance cost, etc. have actually caused the necessity of new long distance Night View equipment. Moreover there might follow lots of difficulties in long-distance visualization in the event that irregular reflection is generated by minute particles in the atmosphere such as fog, smog, and dust, etc. These factors are motivate the work presented in this study. Our study is aimed at the realization of Pulsed Laser Illuminator and newly proposed Range-Gated image acquisition technology. And also the implementation of Tracker for continuous trace of the objects of interest from the obtained sequence images.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2017.10a
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• pp.510-511
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• 2017

In this paper, we have studied to minimize the lifetime and fault occurrence of LED fixtures, which have a short lifetime and frequent faults. In the current LED chip, the lifetime is semi-permanent, but compared to the lifetime of the LED chip, Drivers do not last long. In recent years, low-priced LED illuminators such as those from China have entered the market, and many consumers are perceiving LED illuminators. In order to solve these problems, we designed an LED illuminator of 25W class by using AC Direct IC, which has a longer lifetime than the conventional driver, by removing the electrolytic capacitor in the LED driving driver which is the cause of the failure.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.43 no.2 s.308
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• pp.19-29
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• 2006

As the security of personal information is becoming more important in mobile phones, we are starting to apply iris recognition technology to these devices. In conventional iris recognition, magnified iris images are required. For that, it has been necessary to use large magnified zoom & focus lens camera to capture images, but due to the requirement about low size and cost of mobile phones, the zoom & focus lens are difficult to be used. However, with rapid developments and multimedia convergence trends in mobile phones, more and more companies have built mega-pixel cameras into their mobile phones. These devices make it possible to capture a magnified iris image without zoom & focus lens. Although facial images are captured far away from the user using a mega-pixel camera, the captured iris region possesses sufficient pixel information for iris recognition. However, in this case, the eye region should be detected for accurate iris recognition in facial images. So, we propose a new fast iris detection method, which is appropriate for mobile phones based on corneal specular reflection. To detect specular reflection robustly, we propose the theoretical background of estimating the size and brightness of specular reflection based on eye, camera and illuminator models. In addition, we use the successive On/Off scheme of the illuminator to detect the optical/motion blurring and sunlight effect on input image. Experimental results show that total processing time(detecting iris region) is on average 65ms on a Samsung SCH-S2300 (with 150MHz ARM 9 CPU) mobile phone. The rate of correct iris detection is 99% (about indoor images) and 98.5% (about outdoor images).

• Korean Journal of Food Science and Technology
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• v.28 no.4
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• pp.786-789
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• 1996

An image processing system was built to evaluate the color properties of apple and meat. The system consisted of video camera, video card, 32 bit microcomputer and an optical illuminator. The operating software was developed to carry out capturing, analyzing, displaying and storing of the 8 bit digitized images of food. The images of apples at various maturing stages were investigated to obtain the color histogram of R, G, B and Hunter value. RGB histogram showed a major difference in G value, 35.01, the minor change in R value, 6.16, and the negligible difference in B value. The image of beef cut was separated into two parts, fat and lean tissue, by applying threshold value method based on the digital value of color. The threshold value for fat was over 240 and for lean under 230 in R value, respectively. The resulting non fat image showed 2% decreased color difference value, ${\Delta}E$, than whole meat cut.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.45 no.2
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• pp.49-64
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• 2008

Recently, many researches about making more comfortable input device based on gaze detection technology have been done in human computer interface. However, the system cost becomes high due to the complicated hardware and there is difficulty to use the gaze detection system due to the complicated user calibration procedure. In this paper, we propose a new gaze detection method based on the 2D analysis and a simple user calibration. Our method used a small USB (Universal Serial Bus) camera attached on a HMD (Head-Mounted Display), hot-mirror and IR (Infra-Red) light illuminator. Because the HMD is moved according to user's facial movement, we can implement the gaze detection system of which performance is not affected by facial movement. In addition, we apply our gaze detection system to 3D first person shooting game. From that, the gaze direction of game character is controlled by our gaze detection method and it can target the enemy character and shoot, which can increase the immersion and interest of game. Experimental results showed that the game and gaze detection system could be operated at real-time speed in one desktop computer and we could obtain the gaze detection accuracy of 0.88 degrees. In addition, we could know our gaze detection technology could replace the conventional mouse in the 3D first person shooting game.

• Journal of Chest Surgery
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• v.34 no.8
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• pp.611-614
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• 2001

To determine the efficacy and safety of endoscopic resection and ablation of superficial varicosities using a powered vein resector, irrigated illuminator. Material and Method: 83 consecutive patients were involved in the study. 103 limbs in 83 patients were treated using a minimal invasive, powered, vein resecting devide with cutaneous transillumination and tumescent anesthesia technique. There were 51 women and 32 men. All patients were operated under general anesthesia or regional anesthesia. Operative time and patient satisfaction scores were recorded along with the number of incisions made. Result: 83 patients(51/61.4% female, 32/38.6% male, aged 25-78 years) had varicose vein. Average age at the time of operation was 45 years(range, 25 to 78 years). There were 63 unilateral procedures and 20 bilateral operations. Operative time ranged from 24 to 46 minutes (average 35.3 minutes) in the unilateral procedure. The number of incisions per limb averaged 2.7(range, 2 to 5). Postoperative complications occurred in 7(8.4%) patients. Patients were asked to describe their pain on an analog scale ranging form 1 to 10 with r representing no pain and 10 worst imaginable pain. Immediately postoperative pain score was 2.4 Postoperative pain score at 72 hours had a mean score of 2.0. Postoperative pain score at 1 months were 1. Conclusion: Varicose vein removal using Transilluminated Powered Phlebectomy(TIPP) is a safe and efficient procedure. The procedure saves time, is easy to perform, and gives direct visualization and a distinct endpoint of the removal of veins. It is also less tedious to perform and gives good cosmetic results with significant pain relief.

• Journal of the Institute of Electronics and Information Engineers
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• v.51 no.11
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• pp.221-228
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• 2014

The color and shape of the tongue reflect the physiological and clinico-pathological condition of the body. Recently, various tongue image acquisition devices have been developed for accurate diagnosis based on quantitative and objective tongue features. Since a color information is essential for tongue diagnosis, the performance of an illuminator is very important for the tongue image acquisition device. In this study, we developed an indirect illumination, which is possible to improve a homogeneity of light intensities on the tongue surface, and evaluated its performances. In order to realize the indirect illumination (II), a semi-ellipsoidal solid structure (SESS) for the light reflex was located in the system, and two high-brightness white LEDs were placed for illuminating the areas under frontal camera in the SESS. The tongue surface was illuminated by reflected light from the SESS. The light homogeneity induced by three different illuminations including the II was evaluated by calculating coefficient of variation (CV) of illuminance of five regions. The II showed less than 0.01 of CV and the direct illumination (DI) and the direct illumination with a light diffusion plate (DILDP) showed 0.16 and 0.13, respectively. The reflexed pixel ratios of tongue phantom images show 5.76%, 4.22%, 1.79% for the DI, the DILDP and the II, respectively. The homogeneity of a tongue phantom was evaluated by calculating CV of mean pixel values of six different tongue regions, and showed less than 0.06 in the II. If the II technique apply to tongue diagnosis system, it is expected to improve diagnostic accuracy in clinic.