• Journal of Korean Academy of Nursing Administration
• /
• v.8 no.2
• /
• pp.261-271
• /
• 2002

Purpose: This phenomenological study is aimed at illuminating the lived experiences of newly-qualified nurses who working in general hospitals in C province. Method: The data was collected through unstructured, in-depth interviews with 10 new nurses from April to June 2001, and analysed by Colazzi' methodology. Results: Thematic content analysis identified 6 central themes in this experience-difficulties m making interpersonal relationship, negative feelings, fatigue and unskilled work performance, self-reflection, a sense of accomplishment, identity formation as a nurse. Also, this themes were constructed into 6 constructs explaining new nurses' experience. The results may contribute to a better understanding of what new nurses experienced and how their negative conflicts could be managed. Conclusion: New nurses need to get more education and supportive empowerment regarding diverse conflicts during training period. Educational programs like a preceptorship or individual counselling should be developed and adopted to all clinical settings.

• Asian Pacific Journal of Cancer Prevention
• /
• v.16 no.11
• /
• pp.4477-4487
• /
• 2015

In this review we summarize the results of studies employing high-throughput methods of profiling of HPV-associated cervical intraepithelial neoplasia (CIN) and squamous cell cervical cancers at key intracellular regulatory levels to demonstrate the unique identity of the landscape of molecular changes underlying this oncopathology, and to show how these changes are related to the 'natural history' of cervical cancer progression and the formation of clinically significant properties of tumors. A step-wise character of cervical cancer progression is a morphologically well-described fact and, as evidenced by genome-wide screenings, it is indeed the consistent change of the molecular profiles of HPV-infected epithelial cells through which they progressively acquire the phenotypic hallmarks of cancerous cells. In this sense, CIN/cervical cancer is a unique model for studying the driving forces and mechanisms of carcinogenesis. Recent research has allowed definition of the whole-genome spectrum of both random and regular molecular alterations, as well as changes either common to processes of carcinogenesis or specific for cervical cancer. Despite the existence of questions that are still to be investigated, these findings are of great value for the future development of approaches for the diagnostics and treatment of cervical neoplasms.

• International Journal of Costume and Fashion
• /
• v.13 no.2
• /
• pp.15-29
• /
• 2013

Today's technology enables consumers to trade millions of dollars, conduct online banking, access entertainment, and do countless other activities at the click of a button. Online social networks (OSN) have become a cultural phenomenon that allows for individualistic consumerism. Consumers are increasingly utilizing OSN to share ideas, build communities, and contact fellow consumers who are similar to themselves. The relevance of online communities to the music is immense especially because musicians are now using social media to build global audiences. Not only is information about music and performance disseminated online, but musical commodities are sold and traded online. Online music communities allow consumers to elect and create new identities online through the purchase of subcultural commodities. Given the growing economic importance of online music communities it is important to get a holistic view of subcultural communities online. This study utilized content analysis of online music community websites using the Netnography methodology as developed by Kozinet for data collection to analyze consumers' purchasing and consumption behavior of subcultural commodities online as related to the formation of subcultural identities. Findings showed that subcultural items are predominantly purchased online, especially digital music, and there is a need for more custom craft items. The authors presented a new conceptual taxonomy of online subcultural consumer classifications based on online behavior patterns.

• Journal of Integrative Natural Science
• /
• v.11 no.2
• /
• pp.121-129
• /
• 2018

CXCR6 is a major target in drug design as it is a determinant receptor in many diseases like AIDS, Type I Diabetes, some cancer types, atherosclerosis, tumor formation, liver disease and steatohepatitis. In this study, we propose the active site residues of CXCR6 molecule. We employed homology modelling and molecular docking approach to generate the 3D structure for CXCR6 and to explore its interaction between the antagonists and agonists. 3D models were generated using 14 different templates having high sequence identity with CXCR6. Surflex docking studies using pyridine and pyrimidine derivatives enabled the analysis of the binding site and finding of the important residues involved in binding. 3D structure of CXCL16, a natural ligand for CXCR6, was modelled using PHYRE and protein - protein docking was performed using ClusPro. The residues which were found to be crucial in interaction with the ligand are THR110, PHE113, TYR114, GLN160, GLN195, CYS251 and SER255. This study can be used as a guide for therapeutic studies of human CXCR6.

• The Bulletin of The Korean Astronomical Society
• /
• v.42 no.1
• /
• pp.33.2-33.2
• /
• 2017

Symbiotic stars and quasars show strong far UV resonance doublets including O VI 1032 and 1038, which are known to be major coolants of astrophysical plasma with high temperature T > $10^5K$. We investigate the transfer of $H{\alpha}$ and $Ly{\beta}$ in an emission nebula of temperature T ~ $10^5$, where n=2 population is significant. Line photons of $H{\alpha}$ and $Ly{\beta}$ are transferred in the medium through spatial and frequency diffusion altering their identity according to the branching ratios. We adopt a Monte Carlo technique to describe the transfer of $H{\alpha}$ and $Ly{\beta}$ in an emission nebula with a uniform density and a simple geometrical figure. We find that the temperature of the emission nebula is the major controlling parameter to produce a nonnegligible flux of $Ly{\beta}$. In particular, when T exceeds $10^5K$ the number flux ratio may reach ~ 25% with line center optical depth of a few. We discuss the formation of broad $H{\alpha}$ wings from Raman scattering of $Ly{\beta}$ emergent from a hot emission nebula.

• BMB Reports
• /
• v.44 no.3
• /
• pp.170-175
• /
• 2011

We identified a bovine $B_{12}$ trafficking chaperone bCblC in Bos taurus that showed 88% amino acid sequence identity with a human homologue. The protein bCblC was purified from E. coli by over-expression of the encoding gene. bCblC bound cyanocobalamin (CNCbl), methylcobalamin (MeCbl) and adenosylcobalamin (AdoCbl) in the base-off states and eliminated the upper axial ligands forming aquo/hydroxocobalamin ($OH_2$/OHCbl) under aerobic conditions. A transition of $OH_2$/OHCbl was induced upon binding to bCblC. Interestingly, bCblC-bound $OH_2$/OHCbl did not react with reduced glutathione (GSH), while the reaction of free$OH_2$/OHCbl with GSH resulted in the formation of glutathionylcobalamin (GSCbl) and glutathione disulfide (GSSG). Furthermore we found that bCblC eliminates the GSH ligand of GSCbl forming $OH_2$/OHCbl. The results demonstrated that bCblC is a $B_{12}$ trafficking chaperone that binds cobalamins and protects $OH_2$/OHCbl from GSH, which could be oxidized to GSSG by free $OH_2$/OHCbl.

• Journal of the Korean Institute of Rural Architecture
• /
• v.16 no.1
• /
• pp.87-94
• /
• 2014

The objective of this study is to provide a townscape design guideline that harmonized with a historic landscape via condition investigation and landscape analysis of the cultural /commercial environment district where an improvement is a necessity. The followings are the conclusions: 1.To enhance the identity of Gyeongju, should pursue diversity in unity by making the image of architectural landscape a similar peculiarity. 2.The central commercial district where the tradition is valued and contemporary figures meet the tradition needs a landscape formation by CONTEXT. 3.Since a characteristic of the target area is a regional commercial center, to reduce its congestion, reforming the area to simple environment is required. 4.Induce buildings located on the streets with cultural properties to have Korean traditional tiled roof in order to fit in with their surroundings. 5.Make it a rule to partition a wall into tripartition, the wall surface exposed should be the same finishing materials. 6.Consider the visual aspect of pedestrians and a building's width, the recommendable height of the facade should be 3~3.6m. 7.For the design archetype of cornice, four traditional types based on Korean traditional eaves are suggested. 8.The design of signboards should break existing square-shapes, and seek a design to take advantage of icons that historicity and traditionalist of the city are expressed.

• Korean Institute of Interior Design Journal
• /
• v.22 no.1
• /
• pp.274-282
• /
• 2013

This study aims to investigate planning characteristics of common and living space in the dormitory buildings on campus in Chungcheong province. A document research method was used to analyze and classified into floor-plan, zoning and area. Also site visits were conducted at 8 dormitory buildings of 4 universities, and drawings were collected and compared for this research. The results of this study were as follows; 1)Most floor plans of public spaces had corridor types and integrated public space types. The typical floorplan of living space was 2person-1bedroom and apartment type which shared livingroom, bathroom, restroom and shower between bedrooms. The living space type of dormitory buildings was more complex and growth in size. Unit community combined 2-4person per 1bedroom in floor plan can help student with identity and affiliated formation. 2)The types of the spatial composition in public spaces were devided into three zones; study, living and administrative zones such as seminar room, public bathroom and storage, etc. This results shows that various types of public space for the improvement of community of small group has recently become more important than basic sanitary space in public space of dormitory buildings on campus.

• Journal of Microbiology and Biotechnology
• /
• v.18 no.12
• /
• pp.1884-1889
• /
• 2008

The ${\beta}$-lactamase inhibitory protein, BLIP-II, found in the culture supernatant of Streptomyces exfoliatus SMF19, shows no discernible sequence identity with other ${\beta}$-lactamase inhibitory proteins identified in Streptomyces spp. A null mutant of the gene encoding BLIP-II (bliB::$hyg^r$) showed a bald appearance on solid media. Although BLIP-II was initially isolated from the supernatant of submerged cultures, sites of BLIP-II accumulation were seen in the cell envelope. Mutation of bliB was also associated with changes in the formation of septa and condensation of the chromosomal DNA associated with sporulation. The bliB mutant exhibited infrequent septa, showing dispersed chromosomal DNA throughout the mycelium, whereas the condensed chromosomes of the wild-type were separated by regularly spaced septa giving the appearance of a string of beads. Therefore, on the basis of these results, it is suggested that BLIP-II is a regulator of morphological differentiation in S. exfoliatus SMF19.

• Mycobiology
• /
• v.34 no.3
• /
• pp.154-157
• /
• 2006

A fungal isolate collected from infected paprika (Capsicum annuum var. grossum) was characterized as Sclerotinia sclerotiorum based on its ability of sclerotium formation, physiological and molecular properties. When the isolate was grown on potato dextrose agar, oatmeal agar, and malt extract agar, it grew most well on PDA. Optimal temperature and pH for its growth were $25^{\circ}C$ and pH 7, respectively. The fungal isolate produced sclerotia on PDA within 10 days, and the color and shape of the sclerotia were similar to those of S. sclerotiorum. The ITS rDNA regions including ITS1 and ITS2 and 5.8S sequences were amplified using ITS1F and ITS4 primers from the genomic DNAs of the paprika isolate and other known pathogenic S. sclerotiorum isolated from different crops in Korea, and their nucleotide sequences were determined. Sequence comparison analysis showed the ITS rDNA of the paprika isolate shares 100% sequence identity with those of S. sclerotiorum isolated from red pepper, lettuce and a S. sclerotiorum isolate registered in GenBank DNA database. Neighbor joining analysis based on the ITS rDNA sequence revealed the paprika isolate has very close phylogenetic relationships with known Sclerotinia sclerotiorum isolates. This is the first report that S. sclerotiorum has been found associated with paprika rot in paprika growing countries.