• Asian-Australasian Journal of Animal Sciences
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• 제20권5호
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• pp.795-801
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• 2007

Patients with Type I diabetes mellitus have been treated with porcine insulin for several decades and pigs have recently been deemed an ideal source of microencapsulated islet cells for clinical xenotransplantation. In this study, neonatal pigs were anesthetized and sacrificed prior to a pancreatectomy. Islet cells were isolated from pancreas via collagenase digestion. Islet cells were separated and collected by hand under microscopic guidance. These cells were suspended in 1.4% sodium alginate solution and encapsulated by dropping them into 1.1% calcium chloride solution and in which the round gel in size was 250-400 ${\mu}m$ in diameter. Viability of the microencapsulated islet cells cultured in medium at $37^{\circ}C$ was assessed by MTT assay. Furthermore, insulin released in response to glucose challenge was investigated using an enzyme-linked immunosorbent assay. Secretion of insulin was low in response to the basal glucose solution (4.4 mM) in medium and was significantly higher in response to the high glucose solution (16.7 mM). The viability of microencapsulated islet cells did not differ significantly over a period of 7 days; that is, the increasing pattern of insulin concentration in the culture medium after glucose stimulation interval day was similar throughout the 7 days cultivation. In summary, experimental evidences indicated that the effects of alginate-microencapsulation prolonged survival of the neonatal porcine islets in vitro cultures and the insulin response to glucose of the islets was maintained.

• Fisheries and Aquatic Sciences
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• 제24권2호
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• pp.63-77
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• 2021

The aim of this research was to investigate different factors, including cryoprotective agents (CPAs), diluents, dilution ratios, equilibrium times, freezing rates, and thawing methods to optimize cryopreservation protocols for large yellow croaker (Larimichthys crocea). The parameters evaluated were sperm motility, sperm activity index (SAI), survival rate, and DNA damage. Different types of CPAs, such as dimethyl sulfoxide (DMSO), propylene glycol (PG), ethylene glycol (EG), methanol, and glycerol, were tested for sperm preservation. The highest motility, SAI, and survival rate were observed when EG was used. Different diluents such as Stein's solution, Hank's balanced salt solution, marine fish Ringer's solution, artificial seminal plasma (ASP) of small yellow croaker, and Cortland solution were investigated. The highest post-thaw motility was observed upon using ASP as the diluent. Different concentrations of EG were then mixed with ASP to identify the optimal EG concentration. Experimental results showed that the motility (70.33 ± 1.20%), SAI (5), and survival rate (78.30 ± 0.42%) of post-thaw sperm were optimum when 10% EG and ASP were used as the CPA and diluent of cryopreservation, respectively. Post-thaw sperm motility was high at equilibration times below 150 s and at an optimum dilution ratio of 1:1 (sperm: CPA + diluent) and was not significantly different compared with fresh sperm motility. The freezing rate was found to be slow below -10℃/min. The thawing temperature of 45℃ was identified as ideal. The percentage of tail DNA in post-thaw sperm at 10% EG and ASP was also investigated and was found to have more significant DNA damage than that in fresh sperm but significantly lower damage than that in post-thaw sperm at EG concentrations of 5%, 15%, and 20% (p < 0.05). The cryopreservation protocols obtained in this study will be useful in large yellow croaker hatcheries.

• Journal of the Korea Society of Computer and Information
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• 제14권10호
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• pp.1-10
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• 2009

Precise branch predictor has a profound impact on system performance in modern processor architectures. Recent works show that prediction latency as well as prediction accuracy has a critical impact on overall system performance as well. However, prediction latency tends to be overlooked. In this paper, we propose Branch Pre-Prediction policy to tolerate branch prediction latency. The proposed solution allows that branch predictor can proceed its prediction without any information from the fetch engine, separating the prediction engine from fetch stage. In addition, we propose newly modified BTE structure to support our solution. The simulation result shows that proposed solution can hide most prediction latency with still providing the same level of prediction accuracy. Furthermore, the proposed solution shows even better performance than the ideal case, that is the predictor which always takes a single cycle prediction latency. In our experiments, IPC improvement is up to 11.92% and 5.15% in average, compared to conventional predictor system.

• Geomechanics and Engineering
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• 제10권4호
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• pp.423-436
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• 2016

Currently, there is a great interest in the coupling between multiphase fluid flow and geomechanical effects in hydrocarbon reservoirs and surrounding rocks. The ideal solution for this coupled problem is to introduce the geomechanical effects through the stress analysis solution and implement an algorithm, which assures that the equations governing the flow and stress analyses are obeyed in each time step. This paper deals with the implementation of a program (FORTRAN90 interface code), which was developed to couple conventional reservoir (ECLIPSE) and geomechanical (ABAQUS) simulators, using a partial coupling algorithm. The explicit coupled hydro-mechanical behavior of Iranian field during depletion and $CO_2$ injection is studied using the soils consolidation procedure available in ABAQUS. Time dependent reservoir pressure fields obtained from three dimensional compositional reservoir models were transferred into finite element reservoir geomechanical models in ABAQUS as multi-phase flow in deforming reservoirs cannot be performed within ABAQUS. The FEM analysis of the reservoir showed no sign of plastic strain under production and $CO_2$ injection scenarios in any part of the reservoir and the stress paths do not show a critical behavior.

• The KSFM Journal of Fluid Machinery
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• 제7권2호
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• pp.7-13
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• 2004

In an attempt to solve multiobjective optimization problems, weighted sum method is most widely used for the advantage that a designer can consider the relative significance of each object functions by weight values but it can be highly sensitive to weight vector and occasionally yield a deviated optimum from the relative weighting values designer designated because the multiobjective function has the form of simple sum of the product of the weighting values and the object functions in traditional approach. To search the design solution agree well to the designer's weighting values, we proposed new multiobjective function which was the functional of each normalized objective functions and considered to find the design solution comparing the distance between the characteristic line and the ideal optimum. In this study, proposed multiobjective function was applied to design high efficiency and low noise axial flow fan and the result shows this approach is effective for the case that the quality of the design can be highly affected by the designer's subjectiveness represented as weighting values in multiobjective design optimization process.

• Structural Engineering and Mechanics
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• 제44권5호
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• pp.681-704
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• 2012

The dynamic response of Euler-Bernoulli beams to resonant harmonic moving loads is analysed. The non-dimensional form of the motion equation of a beam crossed by a moving harmonic load is solved through a perturbation technique based on a two-scale temporal expansion, which permits a straightforward interpretation of the analytical solution. The dynamic response is expressed through a harmonic function slowly modulated in time, and the maximum dynamic response is identified with the maximum of the slow-varying amplitude. In case of ideal Euler-Bernoulli beams with elastic rotational springs at the support points, starting from analytical expressions for eigenfunctions, closed form solutions for the time-history of the dynamic response and for its maximum value are provided. Two dynamic factors are discussed: the Dynamic Amplification Factor, function of the non-dimensional speed parameter and of the structural damping ratio, and the Transition Deamplification Factor, function of the sole ratio between the two non-dimensional parameters. The influence of the involved parameters on the dynamic amplification is discussed within a general framework. The proposed procedure appears effective also in assessing the maximum response of real bridges characterized by numerically-estimated mode shapes, without requiring burdensome step-by-step dynamic analyses.

• KIEAE Journal
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• 제5권2호
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• pp.19-26
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• 2005

Every site has a different given geometrical and climatic condition, which influenced not only the lifestyle of the humanbeings but also the regional architecture. For example, on a cold region, the reduction of the energy loss is necessary, like an igloo, which has a littlest energy loss at hemisphere. Or on a warm region, the house must be protected thermally from the overheating at the sunshining. like a huge shading. An architectural interpretation in the (extreme) moderate climate, like Korea, has always tried to satisfy the both opposite demands simultaneously. A facade, which divides out- and inside, has an ideal position to lead the regulated regional climatic conditions into the room. The Double Facade System(DFS) is well known as an innovative solution in the european countries, like Germany. It provides an reasonable alternative, which can achieve these goals at the same time. A Double Facade System provides an effective sunshade, which means a cooling energy reduction at the warm season. In addition, it enables a natural ventilation at the cold season with the preheating at the system as well as spring and autumn. An ordinary Single Facade System with a inside or outside sun blind provides a solution just for a specified season, like a summer or winter. But the Double Facade System can deal with the various climatic conditions in the moderate climate.

• International Journal of Naval Architecture and Ocean Engineering
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• 제10권4호
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• pp.439-449
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• 2018

In this paper, multi-objective optimization of a multi-bubble pressure cabin in the underwater glider with Blended-Wing-Body (BWB) is carried out using Kriging and the Non-dominated Sorting Genetic Algorithm (NSGA-II). Two objective functions are considered: buoyancy-weight ratio and internal volume. Multi-bubble pressure cabin has a strong compressive capacity, and makes full use of the fuselage space. Parametric modeling of the multi-bubble pressure cabin structure is automatic generated using UG secondary development. Finite Element Analysis (FEA) is employed to study the structural performance using the commercial software ANSYS. The weight of the primary structure is determined from the volume of the Finite Element Structure (FES). The stress limit is taken into account as the constraint condition. Finally, Technique for Ordering Preferences by Similarity to Ideal Solution (TOPSIS) method is used to find some trade-off optimum design points from all non-dominated optimum design points represented by the Pareto fronts. The best solution is compared with the initial design results to prove the efficiency and applicability of this optimization method.

• Hwankyungkyoyuk
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• 제16권2호
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• pp.29-38
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• 2003

The main purpose of this study is to search a new perspective of environmental education through investigating of alternative education. Environmental education is introduced by needs of society for an effective way of solving environmental problems. But it also has a meaningfulness which is internal need of education for proposing and fostering ecological human corresponds with crisis of modernism. In spite of excessive rhetoric, vision of formal environmental education is very negative. Formal environmental education is restricted to optional course. So, its potential isn't fulfil. Education is both part of the problem and the solution. Education is proclaimed at high level as the key to a more sustainable society and yet it daily plays a part in reproducing an unsustainable society. If environmental education is to fulfil its potential as an agent of environmental problem solution, it becomes both part of the ideal and the practice of education. In this point, alternative education is a model of what environmental education ought to be. Environmental education and alternative education have a common ground in end of education and critical position to the modern education system.

• Computers and Concrete
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• 제21권4호
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• pp.441-449
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• 2018

FE models for complex or large-scaled structures that need detailed modeling of structural components are usually constructed using commercial analysis softwares. Updating of such FE model by conventional sensitivity-based methods is difficult since repeated computation for perturbed parameters and manual calculations are needed to obtain sensitivity matrix in each iteration. In this study, an FE model updating procedure avoiding such difficulties by using response surface (RS) method and a Pareto-based multiobjective optimization (MOO) was formulated and applied to FE models constructed with a commercial analysis package. The test building is a low-rise reinforced concrete building that has been seismically retrofitted. Dynamic properties of the building were extracted from vibration tests performed before and after the seismic retrofits, respectively. The elastic modulus of concrete and masonry, and spring constants for the expansion joint were updated. Two RS functions representing the errors in the natural frequencies and mode shape, respectively, were obtained and used as the objective functions for MOO. Among the Pareto solutions, the best compromise solution was determined using the TOPSIS (Technique for Order of Preference by Similarity to Ideal Solution) procedure. A similar task was performed for retrofitted building by taking the updating parameters as the stiffness of modified or added members. Obtained parameters of the existing building were reasonably comparable with the current code provisions. However, the stiffness of added concrete shear walls and steel section jacketed members were considerably lower than expectation. Such low values are seemingly because the bond between new and existing concrete was not as good as the monolithically casted members, even though they were connected by the anchoring bars.