• Structural Engineering and Mechanics
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• 제21권5호
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• pp.519-537
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• 2005

A new generalized Bernoulli/Timoshenko beam-column element on a two-parameter elastic foundation is presented herein. This element is based on the exact solution of the differential equation which describes the deflection of the axially loaded beam resting on a two-parameter elastic foundation, and can take into account shear deformations, semi - rigid connections, and rigid offsets. The equations of equilibrium are formulated for the deformed configuration, so as to account for axial force effects. Apart from the stiffness matrix, load vectors for uniform load and non-uniform temperature variation are also formulated. The efficiency and usefulness of the new element in reinforced concrete or steel structures analysis is demonstrated by two examples.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.202-205
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• 2000

A gene coding $endo-{\beta}$,-1, 4 glucanase from Actinomyces sp. KNG40 and phytase from Hansenula polymorpha were cloned into Esherichia coli JM101 by using E. coli/Lactobacillus shuttle vector pNZ3004 and pNZ123. The plasmid p3PS(1-4) and p123(1-4) have slpA promoter and slpA signal sequence. So, I constructed expression vectors, p3PS(1-4)Endo, phy and p123(1-4)Endo, phy. These constructed vector was transformed in target host Lactobacillus gasseri and reutri. These transformed host expressed endoglucanase and phytase as extracellular fraction. In the enzyme activity of the same vector, host L, gasseri was higher activity than L. reuteri. This indicates that L. gasseri recongnize promoter and signal sequence very well.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.669-671
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• 2000

Antimicrobial cationic peptides have attracted increasing research and clinical interest as a natural antibiotics due to their broad spectrum of antimicrobial activites and the rapid development of multidrug-resistant pathogenic microorganisms. In this study, first, we synthesized artificial fusion partner and cationic peptide genes (lactoferricin, magainin, protegrin-1, and indolicidin). Second, we constructed recombinant expression vectors and then transformed Pichia pastoris. Finally, expressed cationic peptides were purified and tested for their antimicrobial activites. Antimicrobial activity has been tested upon the appearance of clearing zone on the plate with the lawn of gram negative E.coli XL- I blue and garm positive Staphylococcus aureus. Protegrin-1 and Indolicidin have apparant activity of cationic peotides. This fusion technique may lead to a general and suitable tool for production of pure antimicrobial cationic peptides in Pichia pastoris.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2000년도 추계종합학술대회 논문집(2)
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• pp.59-62
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• 2000

As the density of memories increases, unwanted interference between cells and coupling noise between bit-lines are increased and testing high density memories for a high degree of fault coverage can require either a relatively large number of test vectors or a significant amount of additional test circuitry. From now on, conventional test algorithms have focused on faults between neighborhood cells, not neighborhood bit-lines. In this paper, a new algorithm for NPSFs, and neighborhood bit-line sensitive faults (NBLSFs) based on the NPSFs are proposed. Instead of the conventional five-cell and nine-cell physical neighborhood layouts to test memory cells, a three-cell layout which is minimum size for NBLSFs detection is used. To consider faults by maximum coupling noise by neighborhood bit-lines, we added refresh operation after write operation in the test procedure(i.e., write \longrightarrow refresh \longrightarrow read). Also, we present properties of the algorithm, such as its capability to detect stuck-at faults, transition faults, conventional pattern sensitive faults, and neighborhood bit-line sensitive faults.

• Journal of Mechanical Science and Technology
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• 제18권2호
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• pp.294-301
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• 2004

The natural convective heat transfer in a rectangular enclosure with a heating source has been studied by experiment and numerical analysis. The governing equations were solved by a finite volume method, a SIMPLE algorithm was adopted to solve a pressure term. The parameters for the numerical study are positions and surface temperatures of a heating source i.e., Y /H =0.25, 0.5, 0.75 and 11$^{\circ}C$ $\leq$ΔT$\leq$59$^{\circ}C$. The results of isotherms and velocity vectors have been represented, and the numerical results showed a good agreement with experimental values. Based on the numerical results, the mean Nusselt number of the rectangular enclosure wall could be expressed as a function of Grashof number.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2008년도 추계학술대회A
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• pp.827-831
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• 2008

In this paper, kinematic analysis for a planar 3-DOF redundant stage which has four actuators is presented by using SimMechanics software package. SimMechanics is a block sets of the Matlab/Simulink package. The SimMechanics enables a simplified model for a complex kinematic mechanism, since kinematic relationship between joints and linkages for the kinematic chains are expressed as line vectors and block diagrams. Here, positional error and limit values of movement ranges of the stage are evaluated by using the SimMechanics. The validity of the kinematic characteristics model was compared with theoretical kinematic analyses for the 3-DOF stage.

• Asian Pacific Journal of Cancer Prevention
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• 제17권7호
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• pp.3329-3334
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• 2016

Cancer is thought to be a direct result of transcriptional misregulation. Broad analysis of transcriptional regulatory elements in healthy and cancer cells is needed to understand cancer development. Nucleases regulatory domains are recruited to bind and manipulate a specific genomic locus with high efficacy and specificity. TALENs (transcription activator-like effector nuclease) fused to endonuclease FokI have been used widely to target specific sequences to edit several genes in healthy and cancer cells. This approach is promising to target specific cancer genes and for this purpose it is needed to pack such TALENs into viral vectors. There are some considerations which control the success of this approach, targeting appropriate sequences with efficient construction of TALENs being crucial factors. We face some obstacles in construction of TALENs; in this study we made a modification to the method of Cermk et al 2011 and added one step to make it easier and increase the availability of constructs.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2000년도 춘계학술대회논문집B
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• pp.296-303
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• 2000

Study on natural convection in a rectangular enclosure with a heating point has been studied by numerical and experimental methods. The governing equations were solved by a finite volume method, and for pressure term was used a SIMPLE algorithm. The parameters considered for numerical study are positions and surface temperature of a heating point i.e. Y/H = 0.25. 0.5. 0.75 and $11^{\circ}C{\leq}{\Delta}T{\leq}59^{\circ}C$. The results of isotherms and velocity vectors have been represented for various parameters. Based on the numerical data. the mean Nusselt number in the space can be expressed as a function of Grashof number

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1991년도 추계학술대회
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• pp.57-60
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• 1991

A new approach to texture classification for ultrasound liver diagnosis using run difference matrix was developed. The run difference matrix consists of the gray level difference along with distance. From this run difference matrix, we defined several parameters such as LDE, LDEL, NUF, SMO, SMG, SHP etc. and three vectors namely DOD, DGD and DAD. Each parameter value calculated in fatty cirrhotic, chronic hepatitic and normal liver mage was plotted in two dimensional plane. We compared our results with run length method. There are several advantages of run difference matrix method over the run lengths. 1) It is more sensitive to small difference of gray level distribution. 2) The parameters provide more statistically significant value. Images were classified with the extracted parameters to each diseases using neural networks. In preliminary clinical exprements, this approach showed satisfying results.

• KSBB Journal
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• 제13권6호
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• pp.649-655
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• 1998

Hantaviruses are rodent hosts-borne viruses belonging to the family Bunyaviridae, and are etiologic agents for two acute diseases, i.e., Haemorrhagic Fever with Renal Syndrome (HFRS) and Hantavirus Pulmonary Syndrome (HPS). There have been a lot of reports on prophylactic vaccines and diagnostics for the diseases, but most of viral antigens have been prepared by eukaryotic cell culture. Nucleocapsid proteins of Hantaviruses are known as the major viral antigens. Thereby, we prepared nucleocapsid genes of Hantaan virus and Seoul virus by RT-PCR and cloned into plasmid vectors, pET-3a and pKK223-3. Both genes were expressed in Escherichia coli with higher expression level of Seoul viral nucleocapsid protein compared to that of Hantaan in pET-3a. Hantaan viral gene was expressed much higher level in plasmid pET-3a that in pKK223-3. About 30% of expressed nucleocapsid protein was soluble and the rest was remained in insoluble fraction.