• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제45회 하계 정기학술대회 초록집
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• pp.90.1-90.1
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• 2013

Nature utilizes various of the colorization process. Some species of birds can express their mood of tempers by changing their collagen structures on skin. For example, turkey can change their skin color by expansion of the collagen structures, which are associated with the distinct color changes. Here, we developed bioinspired virus-based colorimetric sensors which can be genetically tuned for target molecule. Using M 13 bacteriophage, we fabricated responsive self-assembled color matrices composed of quasi-ordered fiber bundle structures. These virus matrices can exhibit color change by stimuli through fiber bundle structure modulation. Upon exposure of volatile organic compounds, the resulting multi-colored matrices exhibited distinct color changes with different ratios that can be recognized by the naked eyes. Using the directed evolutionary approaches, we genetically engineered the virus matrix to incorporate binding motif for explosive detection (i.e., trinitrotoluene (TNT)). Through utilizing a common handheld device (i.e., iPhone), we could distinguish TNT molecules down to 20 ppb in a selective manner. Our novel biomimetic virus colorimetric sensor can overcome current limitation for low response selectivity.

• 복식
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• 제61권1호
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• pp.69-83
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• 2011

This study applied cartoon-like simple and implicit symbol of Keith Haring works to tubular jacquard knit, designed woman casual knit wear with fun, explicit and activities using fluorescent yarns with explicit features. This study aimed to propose the directions for new market pioneering that meets the emotional requirements of modern people by raising the level of utilization and expression area of knit fashion, and to present the constructive directions for high value-added knit wear by designing highly effective knit wear. Methods of this study examined the lifetime and artistic background of Keith Haring and collected his works through searching of internet, scholastic publications or thesis. Formative types and colors of works collected were analyzed and classified according to characteristic standards of Haring and characteristic formativeness and color of works were extracted. The result of this study is as shown below: First, the feature of his works can be summarized into motif that has symbolic and implicit cartoon-like lines including narratives as well as enormous number of works. Such feature is very suitable for variation and recombination in realization of design and has unlimited potentials for development. Second, image colors can be extracted such as black and white, original color and fluorescent paints, which reflects the intention of artist who put focus on communication with the public. Such color feature is very suitable for design motif that has explicitness of reflecting the intention of artist through use of special fluorescent yarn. Third, I made it as reversible wear that has effects of increasing or decreasing the explicitness according to color area ratio using the feature of textures allowing use of both sides as well as form stability of tubular jacquard. Fourth, by adjusting the thickness of fabric with controlling of ply in fabric using poly yarn and wool union yarn, I could obtain good results of study in terms of technique that can express various materials with embossed dynamic effects of unevenness on flat surface.

• Asian Pacific Journal of Cancer Prevention
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• 제14권9호
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• pp.5489-5494
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• 2013

In the current study we aimed to show the common YMDD motif mutations in viral polymerase gene in chronic hepatitis B patients during lamivudine and adefovir therapy. Forty-one serum samples obtained from chronic hepatitis B patients (24 male, 17 female; age range: 34-68 years) were included in the study. HBV-DNA was extracted from the peripheral blood of the patients using an extraction kit (Invisorb, Instant Spin DNA/RNA Virus Mini Kit, Germany). A line probe assay and direct sequencing analyses (INNO-LIPA HBV DR v2; INNOGENETICS N.V, Ghent, Belgium) were applied to determine target mutations of the viral polymerase gene in positive HBV-DNA samples. A total of 41 mutations located in 21 different codons were detected in the current results. In 17 (41.5%) patients various point mutations were detected leading to lamivudin, adefovir and/or combined drug resistance. Wild polymerase gene profiles were detected in 24 (58.5%) HBV positive patients of the current cohort. Eight of the 17 samples (19.5%) having rtM204V/I/A missense transition and/or transversion point mutations and resistance to lamivudin. Six of the the mutated samples (14.6%) having rtL180M missense transversion mutation and resistance to combined adefovir and lamivudin. Three of the mutated samples (7.5%) having rtG215H by the double base substituation and resistance to adefovir. Three of the mutated samples (7.5%) having codon rtL181W due to the missense transversion point mutations and showed resistance to combined adefovir and lamivudin. Unreported novel point mutations were detected in the different codons of polymerase gene region in the current HBV positive cohort fromTurkish population. The current results provide evidence that rtL180M and rtM204V/I/A mutations of HBV-DNA may be associated with a poor antiviral response and HBV chronicity during conventional therapy in Turkish patients.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2015년도 추계학술대회 및 정기총회
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• pp.26-26
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• 2015

Phytophthora infestans is the causal agent of potato and tomato late blight, one of the most devastating plant diseases. P. infestans secretes effector proteins that are both modulators and targets of host plant immunity. Among these are the so-called RXLR effectors that function inside plant cells and are characterized by a conserved motif following the N-terminal signal peptide. In contrast, the effector activity is encoded by the C terminal region that follows the RXLR domain. Recently, I performed in planta functional profiling of different RXLR effector alleles. These genes were amplified from a variety of P. infestans isolates and cloned into a Potato virus X (PVX) vector for transient in planta expression. I assayed for R-gene specific induction of hypersensitive cell death. The findings included the discovery of new effector with avirulence activity towards the Solanum bulbocastanum Rpi-blb2 resistance gene. The Rpi-blb2 encodes a protein with a putative CC-NBS-LRR (a coiled-coil-nucleotide binding site and leucine-rich repeat) motif that confers Phytophthora late blight disease resistance. We examined the components required for Rpi-blb2-mediated resistance to P. infestans in Nicotiana benthamiana. Virus-induced gene silencing was used to repress candidate genes in N. benthamiana and to assay against P. infestans infections. NbSGT1 was required for disease resistance to P. infestans and hypersensitive responses (HRs) triggered by co-expression of AVRblb2 and Rpi-blb2 in N. benthamiana. RAR1 and HSP90 did not affect disease resistance or HRs in Rpi-blb2-transgenic plants. To elucidate the role of salicylic acid (SA) in Rpi-blb2-mediated resistance, we analyzed the response of NahG-transgenic plants following P. infestans infection. The increased susceptibility of Rpi-blb2-transgenic plants in the NahG background correlated with reduced SA and SA glucoside levels. Furthermore, Rpi-blb2-mediated HR cell death was associated with $H_2O_2$, but not SA, accumulation. SA affects basal defense and Rpi-blb2-mediated resistance against P. infestans. These findings provide evidence about the roles of SGT1 and SA signaling in Rpi-blb2-mediated resistance against P. infestans.

• BMB Reports
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• 제37권2호
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• pp.254-259
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• 2004

The wild-type and temperature-sensitive (ts) repressor genes were cloned from the temperate mycobacteriophage L1 and its mutant L1cIts391, respectively. A sequencing analysis revealed that the $131^{st}$ proline residue of the wild-type repressor was changed to leucine in the ts mutant repressor. The 100% identity that was discovered between the two DNA regions of phages L1 and L5, carrying the same sets of genes including their repressor genes, strengthened the speculation that L1 is a minor variant of phage L5 or vice versa. A comparative analysis of the repressor proteins of different mycobacteriophages suggests that the mycobacteriophage-specific repressor proteins constitute a new family of repressors, which were possibly evolved from a common ancestor. Alignment of the mycobacteriophage-specific repressor proteins showed at least 7 blocks (designated I-VII) that carried 3-8 identical amino acid residues. The amino acid residues of blocks V, VI, and some residues downstream to block VI are crucial for the function of the L1 (or L5) repressor. Blocks I and II possibly form the turn and helix 2 regions of the HTH motif of the repressor. Block IV in the L1 repressor is part of the most charged region encompassing amino acid residues 72-92, which flanks the putative N-terminal basic (residues 1-71) and C-terminal acidic (residues 93-183) domains of L1 repressor.

• 한국컴퓨터그래픽스학회논문지
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• 제1권2호
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• pp.269-272
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• 1995

본 논문에서는 자연지형의 3차원 투사도 제작기술에 비행기의 비행조건을 고려하여 개발한 비행 시뮬레이터에 대하여 설명하고자 한다. 여기서 발표하고자 하는 비행 시뮬레이터란 3차원의 자연지형을 마치 비행기를 타고가며 보는 것과 같은 효과를 낼 수 있게 하는 시스템을 말한다. 본 시스템은 사용자의 편의성을 도모하기 위하여 Sun workstation (SPARC 10)상에서 X11R5와 Motif 그리고 C언어를 이용하여 개발되었으며, 사용자가 비행경로, 비행속도, 비행고도 등의 비행 파라메타들을 마우스를 이용하여 손쉽게 입력할 수 있도록 구성되어 있다.

• BMB Reports
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• 제39권3호
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• pp.263-269
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• 2006

In the genome of Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus, open reading frame 39 (Ha39) is the only gene predicted to encode an RNA recognition protein. Computer analysis revealed that Ha39 homologues were found in 15 NPVs, but not in GVs. Its transcripts were detected from 3 through 72 hours post infection (h p.i.) using RT-PCR and Northern blot analysis. The protein was detected in infected-cell lysates from 6 h p.i. Western blot assay of ODV and BV preparations revealed that Ha39 encodes a structural protein associated with BVs. Additionally, immunofluorescence microscopy demonstrated that the protein was present within cytoplasm in virus-infected cells, but not in the nuclear region.

• 한국가구학회지
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• 제27권2호
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• pp.96-103
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• 2016

This article is to develop test product as adopting motifs from Andong Hahoe Village registered in the UNESCO World Heritage. This study associates physiographic features and images of Hahoe Village with test product. In the research result of this study, the value of product and possibility of success of test product was found as follows. Firstly, Making the puzzle-oriented Desk Service Hahoe Village views by using 22 different styles & functional artworks. Secondly, Producing the tangible forms such as a tree, thatched cottage, arbor, tile-roofed house and ferryboat by casting technique. Thirdly, Designing the shape of the terrain by utilizing computer programing softwares such as CAD drawings and ARTCAM. Finally, Developing the mass-produce goods reflecting region historical and cultural characteristics. I expect this study may increase of interest of our country's culture and play several roles of the bridgehead for product development. Also I reconsider sensitivity recovery of users and our cultural value increase.

• 한국농공학회지
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• 제39권1호
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• pp.41-47
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• 1997

A geographical resource analysis support system (GRASS) was incorporated to an input and output processor for the agricultural nonpoint source pollution (AGNPS) model. The resulting interface system, GIS-AGNPS was a user-friendly, menu-driven system. GIS-AGNPS was developed to automatically process the input and output data from GIS-based data using GRASS and Motif routines. GIS-AGNPS was consisted of GISAGIN which was an input processor for the AGNPS model, GISAGOUT a output processor for the AGNPS and management submodel. The system defines an input data set for AGNPS from attributes of basic and thematic maps. It also provides with editing modes so that users can adjust and detail the values for selected input parameters, if needed. The post-processor at the system displays graphically the outputs from AGNPS, which may he used to identify areas significantly contributing nonpoint source pollution loads.

• Molecules and Cells
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• 제38권9호
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• pp.759-764
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• 2015

Tripartite motif protein 25 (TRIM25), mediates K63-linked polyubiquitination of Retinoic acid inducible gene I (RIG-I) that is crucial for downstream antiviral interferon signaling. Here, we demonstrate that TRIM25 is required for melanoma differentiation-associated gene 5 (MDA5) and MAVS mediated activation of NF-${\kappa}B$ and interferon production. TRIM25 is required for the full activation of NF-${\kappa}B$ at the downstream of MAVS, while it is not involved in IRF3 nuclear translocation. Mechanical studies showed that TRIM25 is involved in TRAF6-mediated NF-${\kappa}B$ activation. These collectively indicate that TRIM25 plays an additional role in RIG-I/MDA5 signaling other than RIG-I ubiquitination via activation of NF-${\kappa}B$.