• Title/Summary/Keyword: i-motif

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Geometric Figures of Picturesque Gardens (1):$\sqrt{2}$ Dagram in Muskau Park, Germany (독일 풍경식정원의 도형원리(1):무스카우정원과 $\sqrt{2}$도형)

  • 정기호
    • Journal of the Korean Institute of Landscape Architecture
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    • v.25 no.3
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    • pp.124-130
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    • 1997
  • In some cases of the English landscape garden style in Germany, for example, Worlitz, Branitz etc., including Mu나며, I have found out invisible geometric figures, that must be on the basis of landscape gardening. Particularly the Muskau park. The church of the village "Gerg", outside of the park, and the "pucklerstein" are the bases of this diagram. Above all, I am convinced of my hypothesis of √2 diagram, while I can also understand, out of my analysis, the relations and descriptions of the park in the book of the gardner, Hermann Fust von Puckler-muskau, "Andeutungen uber Landscahaftsgartnerei". Finally, I wish to discuss, how to do the phenomena, 'picturesque motif and geometric figures of the English Landscape Garden.

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Characterization of a Chalcosyltransferase (gerGTII) in Dihydrochalcomycin Biosynthesis

  • Pageni, Binod Babu;Oh, Tae-Jin;Thuy, Ta Thi Thu;Sohng, Jae Kyung
    • Molecules and Cells
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    • v.26 no.3
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    • pp.278-284
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    • 2008
  • An open reading frame, designated GerGTII and located downstream of the polyketide synthase genes, has been identified as a chalcosyltransferase by sequence analysis in the dihydrochalcomycin biosynthetic gene cluster of Streptomyces sp. KCTC 0041BP. The deduced product of gerGTII is similar to several glycosyltransferases, authentic and putative, and it displays a consensus sequence motif that appears to be characteristic of a sub-group of these enzymes. Specific disruption of gerGTII within the S. sp. KCTC 0041BP genome by insertional in-frame deletion method, resulted complete abolishment of dihydrochalcomycin and got the 20-O-mycinosyl-dihydrochalconolide as intermediate product in dihydrochalcomycin biosynthesis which was confirmed by electron spray ionization-mass spectrometry and liquid chromatography-mass spectrometry. Dihydrochalcomycin also was recovered after complementation of gerGTII.

Writing papers: literary and scientific

  • Hwang, Kun
    • Journal of Trauma and Injury
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    • v.35 no.3
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    • pp.145-150
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    • 2022
  • This paper aims to summarize why I write, how to find a motif, and how to polish and finish a manuscript. For William Carlos Williams, practicing medicine and writing poetry were two parts of a single whole, not each of the other. The two complemented each other. Medicine stimulated Williams to become a poet, while poetry was also the driving force behind his role as a doctor. Alexander Pope, the 18th century English poet, wrote a poem entitled "The Epistle to Dr. Arbuthnot" that was dedicated to a friend who was both a poet and a physician. In this poem, we receive an answer to the questions of "Why do you write? Why do you publish?" Pope writes, "Happy my studies, when by these approv'd! / Happier their author, when by these belov'd! / From these the world will judge of men and books." When I write, I first reflect on whether I only want to write something for its own sake, like "a dog chasing its own tail," instead of making a more worthwhile contribution. When my colleagues ask me, "Why do you write essays as well as scientific papers?" I usually answer, "Writing is a process of healing for me-I cannot bear myself unless I write." When the time comes to sit down and put pen to paper, I remind myself of the saying, festina lente (in German, Ohne Hast, aber ohne Rast, corresponding to the English proverb "more haste, less speed"). If I am utterly exhausted when I finish writing, then I know that I have had my vision.

Characterization of a Salicylic Acid- and Pathogen-induced Lipase-like Gene in Chinese Cabbage

  • Lee, Kyung-Ah;Cho, Tae-Ju
    • BMB Reports
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    • v.36 no.5
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    • pp.433-441
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    • 2003
  • A cDNA clone for a salicylic acid-induced gene in Chinese cabbage (Brassica rapa subsp. pekinensis) was isolated and characterized. The cabbage gene, designated Br-sil1 (for $\underline{B}$rassica $\underline{r}$apa $\underline{s}$alicylate-$\underline{i}$nduced $\underline{l}$lipase-like 1 gene), encodes a putative lipase that has the family II lipase motif GDSxxDxG around the active site serine. A database search showed that plant genomes have a large number of genes that contain the family II lipase motif. The lipase-like proteins include a myrosinase-associated protein, an anther-specific proline-rich protein APG, a pollen coat protein EXL, and an early nodule-specific protein. The Br-sil1 gene is strongly induced by salicylic acid and a non-host pathogen, Pseudomonas syringae pv. tomato, that elicits a hypersensitive response in Chinese cabbage. Treatment of the cabbage leaves with BTH, methyl jasmonate, or ethephon showed that the Br-sil1 gene expression is induced by BTH, but not by methyl jasmonate or ethylene. This indicates that the cabbage gene is activated via a salicylic acid-dependent signaling pathway. An examination of the tissue-specific expression revealed that the induction of the Br-sil1 gene expression by BTH occurs in leaves and stems, but not in roots and flowers. Without the BTH treatment, however, the Br-sil1 gene is not expressed in any of the tissues that were examined.

Identification and Functional Analysis of SEDL-binding and Homologue Proteins by Immobilized GST Fusion and Motif Based Methods

  • Hong, Ji-Man;Jeong, Mi-Suk;Kim, Jae-Ho;Kim, Boog-il;Holbrook, Stephen R.;Jang, Se-Bok
    • Bulletin of the Korean Chemical Society
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    • v.29 no.2
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    • pp.381-388
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    • 2008
  • An X-linked skeletal disorder, SEDT (spondyloepiphyseal dysplasia tarda) is a genetic disease characterized by a disproportionately short trunk and short stature caused by mutations in the SEDL gene. This gene is evolutionarily conserved from yeast to human. The yeast SEDL protein ortholog, Trs20p, has been isolated as a member of a large multi-protein complex called the transport protein particle (TRAPP), which is involved in endoplasmic reticulum (ER)-to-Golgi transport. The interaction between SEDL and partner proteins is important in order to understand the molecular mechanism of SEDL functions. We isolated several SEDL-binding proteins derived from rat cells by an immobilized GST-fusion method. Furthermore, the SEDL-homologue proteins were identified using motif based methods. Common motifs between SEDL-binding proteins and SEDL-homologue proteins were classified into seven types and 78 common motifs were revealed. Sequence similarities were contracted to seven types using phylogenetic trees. In general, types I-III and VI were classified as having the function of acetyl-CoA carboxylase, glycogen phosphorylase, isocitrate dehydrogenase, and enolase, respectively, and type IV was found to be functionally related to the GST protein. Types V and VII were found to contribute to TRAPP vesicle trafficking.

Analysis of Promoter Elements for Transcriptional Expression of Rat p53 Gene in Regenerating Liver

  • Lee, Min-Hyung;Song, Hai-Sun;Park, Sun-Hee;Choi, Jin-Hee;Yu, Sun-Hee;Park, Jong-Sang
    • BMB Reports
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    • v.32 no.1
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    • pp.45-50
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    • 1999
  • We previously found three transcription factor-binding motifs in the rat p53 promoter. They are two recognition motifs of NF1-like protein (NF1-like element 1: -296 ~ -312, NF1-like element 2: -195 ~ -219) and a bHLH protein binding element (-142 ~ -146). In this study, we investigated the DNA-protein complex formation of the three elements with nuclear extracts from both normal and regenerating liver to find the element involved in the induced transcription of p53. The level of each DNA-protein complex on NF1-like and bHLH motifs was not changed. Instead, a new element located at -264 ~ -284 was detected in the DNase I footprinting assay with regenerating nuclear extract. This element has partial homology to the AP1 consensus motif. However, the competition studies with diverse oligonucleotides suggest that the binding protein is not AP1. An in vitro transcription assay shows that this element is important for the transcriptional activation of the rat p53 promoter. Therefore, for the induced transcription of the rat p53 promoter, the-264 ~ -284 region is required in addition to two NF1-like and one bHLH motif.

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A Study on Multipurpose Fashion Design with the Motif of Surrealist Objects (초현실주의 오브제를 모티프로 한 다목적 패션 디자인에 관한 연구)

  • Eun, Sook;Lee, Youn-Hee;Park, Jae-Ok
    • The Research Journal of the Costume Culture
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    • v.15 no.1 s.66
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    • pp.68-80
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    • 2007
  • Surrealism's method of expression and inspiration has been a continuous influence on fashion. What is important is how to identify and research a source of inspiration, and how to use this inspiration to guide the designs that can be worn on a real human body. Therefore, the purpose of this study was to show how to develop ideas and designs from Surrealist objects and how to contribute to the development of designs to the diversity in visual and functional effects along with the changes in consumer's interest. Documentary studies about Surrealism and Surrealist-inflected fashion applying the works of Surrealist and fashion images as a tool of historical research were proceeded. And the ideas for theme, the motifs for print patterns, and the concept of designs were borrowed from Surrealist objects touching on the imagery between the real and reflected, optical and artificial, and the designs were developed with several different visual and functional effects changing color, pattern, size, and length with the function of zippers in each item. It also gives the person wearing it amusement because it includes the concept of play and D.I.Y. With effective planning, it is possible to attract consumer's interest in the market.

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Identification of Novel Cupredoxin Homologs Using Overlapped Conserved Residues Based Approach

  • Goyal, Amit;Madan, Bharat;Hwang, Kyu-Suk;Lee, Sun-Gu
    • Journal of Microbiology and Biotechnology
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    • v.25 no.1
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    • pp.127-136
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    • 2015
  • Cupredoxin-like proteins are mainly copper-binding proteins that conserve a typical rigid Greek-key arrangement consisting of an eight-stranded β-sandwich, even though they share as little as 10-15% sequence similarity. The electron transport function of the Cupredoxins is critical for respiration and photosynthesis, and the proteins have therapeutic potential. Despite their crucial biological functions, the identification of the distant Cupredoxin homologs has been a difficult task due to their low sequence identity. In this study, the overlapped conserved residue (OCR) fingerprint for the Cupredoxin superfamily, which consists of conserved residues in three aspects (i.e., the sequence, structure, and intramolecular interaction), was used to detect the novel Cupredoxin homologs in the NCBI non-redundant protein sequence database. The OCR fingerprint could identify 54 potential Cupredoxin sequences, which were validated by scanning them against the conserved Cupredoxin motif near the Cu-binding site. This study also attempted to model the 3D structures and to predict the functions of the identified potential Cupredoxins. This study suggests that the OCR-based approach can be used efficiently to detect novel homologous proteins with low sequence identity, such as Cupredoxins.

A Study on Inrormation Analysis and Automaiton of Objects based on Reusability (재사용에 기반한로서 객체들의 정보 분석과 자동화에 관한 연구)

  • Kim, Jae-Saeng;Song, Yeong-Jae
    • The Transactions of the Korea Information Processing Society
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    • v.4 no.2
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    • pp.384-394
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    • 1997
  • Research about Reuse is developed in various direstions, but the sucessful exmples; of the rexuavle objects are very rare in praciice because technical environment to support reuse is short.In this paper we describe a sys-tem for analyxing the information about reusability of C++ objects and for storing and managing the extracted information in information DB.In this development system, user can see the specification information of objects and the quality information of the procedual and object oriented atibutes by browsing.And objests that are not on the reuse unformation DB is analyxed and is tested automatically using the information analyzer by user.We implemented this system using C program in X-Window OSFI/Motif 2.1.1.

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"Entanglement of Echoes in Near / Miss" Bernstein, Charles. Near / Miss Chicago: U of Chicago P, 2018.

  • Feng, Yi
    • Journal of English Language & Literature
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    • v.64 no.2
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    • pp.299-305
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    • 2018
  • Near / Miss, Charles Bernstein's poetry collection, is replete with poems of distinctive styles and pluralistic forms in his idiosyncratic and artistic cosmos. With poetic antics, queerness, sarcasm, irony, and humor, the book showcases the motif of loss, chaos and trauma in postmodern America and the world. The multiplicity and multi-dimensional $M{\ddot{o}}bius$ effect in Near / Miss echo earlier Bernstein's poems, as well as poems by ancient and contemporary poets, with visual artists and musicians, and rabbis and Jewish philosophers. I argue that Near / Miss offers an apotheosis of echopoetics, which has been launched in his previous book Pitch of Poetry. Poems in the book reveal the dark and thick "pitch," namely the queer, the uncanny, the invisible, the disabled, the dispossessed, and the silenced poetic Other and make it explicit. The estrangement and alienation of $clich{\acute{e}}$ through diverse malaprops, mondegreens, non-sequiturs and fragmentations in Near / Miss aim at deconstructing the fixation of language so as to display the poetic Other. The motif of "nothingness" in echopoetics significantly multiplies its meanings. Nothingness mainly refers to the loss of origin, the defiance of tyranny, and the sublimity of the universe and the poetic Other. Melding his personal loss and misfortune, the current political discontent and the postmodern chaos in America and the world, nothingness in echopoetics resonates with American literary tradition and Zen with a healing and transforming power.