• Title/Summary/Keyword: i-FP

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FMN-Based Fluorescent Proteins as Heavy Metal Sensors Against Mercury Ions

  • Ravikumar, Yuvaraj;Nadarajan, Saravanan Prabhu;Lee, Chong-Soon;Jung, Seunho;Bae, Dong-Ho;Yun, Hyungdon
    • Journal of Microbiology and Biotechnology
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    • v.26 no.3
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    • pp.530-539
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    • 2016
  • Bacterial light-oxygen-voltage-sensing photoreceptor-derived flavin mononucleotide (FMN)-based fluorescent proteins act as a promising distinct class of fluorescent proteins utilized for various biomedical and biotechnological applications. The key property of its independency towards oxygen for its chromophore maturation has greatly helped this protein to outperform the other fluorescent proteins such as GFP and DsRed for anaerobic applications. Here, we describe the feasibility of FMN-containing fluorescent protein FbFP as a metal-sensing probe by measuring the fluorescence emission changes of a protein with respect to the concentration of metal ions. In the present study, we demonstrated the mercury-sensing ability of FbFP protein and the possible amino acids responsible for metal binding. A ratiometric approach was employed here in order to exploit the fluorescence changes observed at two different emission maxima with respect to Hg2+ at micromolar concentration. The engineered variant FbFPC56I showed high sensitivity towards Hg2+ and followed a good linear relationship from 0.1 to 3 μM of Hg2+. Thus, further engineering with a rational approach would enable the FbFP to be developed as a novel and highly selective and sensitive biosensor for other toxic heavy metal ions as well.

Effect of Pilates Gymball Exercises on the Electroencephalogram and Cognitive Function in Mentally Disabled Persons

  • Son, Yu-Joung;Lim, Jae-Heon
    • The Journal of Korean Physical Therapy
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    • v.29 no.5
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    • pp.227-233
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    • 2017
  • Purpose: The aim of this study was to determine if Pilates gymball exercise can change the electroencephalogram and cognitive function of mentally disabled people. Methods: Twenty-one mentally disabled people were enrolled in this study. They were assigned randomly to one of two groups: Pilates gymball exercise group (PGEG, n=11), and control group (CG, n=10). The subjects in the PGEG group performed the exercises for 50 minutes a day, three days per week for 6 weeks. The PGEG program consisted of warm up (10 minutes), main workout (30 minutes), and cool down (10 minutes). The main workout consisted of 10 exercise programs. The electroencephalogram (EEG) of Fp1, Fp2, F3, F4, C3, C4, O1, and O2 were measured using an PolyG-I system. The cognitive function was evaluated using a mini-mental state examination (MMSE). The measurements were performed before exercise, and 6 weeks after exercise. Covariance analysis (ANCOVA) was performed to determine the difference between the two groups Results: A significant difference in Fp1, Fp2, and F3 on the relative alpha power was observed between the PGEG and CG groups (p<0.05). A significant difference in Fp1 on the relative beta power was observed between the PGEG and CG groups (p<0.05). No significant difference in the MMSE score was observed between the PGEG and CG groups. Conclusion: Pilates gymball exercise did positively change the EEG in the frontal lobe. On the other hand, the effect related to cognitive was limited. Pilates gymball exercise appears to be more effective in facilitating brain stimulation related to cognition.

A signal processing technique for interferometric fiber-optic sensors (간섭형 광섬유센서의 신호처리 기법)

  • 예윤해
    • Korean Journal of Optics and Photonics
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    • v.6 no.4
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    • pp.365-372
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    • 1995
  • A signal processing technique for interferometric fiber-optic sensors is proposed. It does not require a any special optic components such as phase modulator, $3times3$ couplers,to obtain the full sensitivity of the interferometer. Instead, it requires a reference interferometer for phase referencing and a reference mirror for intensity referencing, but intensity referencing can be done without using the r reference mirror. The new technique utilizes the frequency chirping of the laser diode to process t the sensor signal with both wide dynamic range and high sensitivity of the interferometer. It was a applied to an internal-mirrored FP interferometric temperature sensor to obtain the system noise of $4\times10^{-3\circ}C$ from I cm FP Interferometor sensor device.

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Antecedents and Consequence of Governance Characteristics, Earnings Management, and Company Performance: An Empirical Study in Iraq

  • AHMED, Mohammed Ghanim;GANESAN, Yuvaraj;HASHIM, Fathyah
    • The Journal of Asian Finance, Economics and Business
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    • v.8 no.8
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    • pp.57-66
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    • 2021
  • The outbreak of the financial crisis, the lack of corporate governance practices in Iraqi companies, the high level of earnings management (EM), and weak firm performance (FP) have all encouraged the purpose of this study. This study proposes to achieve the following objectives: (I) to investigate the influence of governance mechanisms on the earnings management practices, (II) to investigate the consequence of EM on FP. The study sample includes 65 Iraqi firms listed on the Iraqi stock exchange for six years from 2012 to 2018, with 390 firm-year observations. The hypotheses were tested using panel data regression. According to the findings, Iraqi companies prefer to use real EM rather than accruals EM to avoid reporting losses. Discretionary cash flow, production costs, and cash flow from operation are examples of actual operations employed to undertake EM. Furthermore, according to the findings of this study, board meeting frequency and female onboard have a significant and negative influence on EM. Besides, the internal audit function was found not to affect EM. On the other hand, results revealed a significant and negative relationship between EM and FP. According to the study, management prefers to minimize cash and accrual expenditure during the economic downturn.

A CERTAIN PROPERTY OF POLYNOMIALS AND THE CI-STABILITY OF TANGENT BUNDLE OVER PROJECTIVE SPACES

  • Tanaka, Ryuichi
    • Bulletin of the Korean Mathematical Society
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    • v.44 no.1
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    • pp.83-86
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    • 2007
  • We determine the largest integer i such that $0 and the coefficient of $t^{i}$ is odd in the polynomial $(1+t+t^{2}+{\cdots}+t^{n})^{n+1}$. We apply this to prove that the co-index of the tangent bundle over $FP^{n}$ is stable if $2^{r}{\leq}n<2^{r}+\frac{1}{3}(2^{r}-2)$ for some integer r.

Finiteness properties of some poincare duality groups

  • Lee, Jong-Bum;Park, Chan-Young
    • Journal of the Korean Mathematical Society
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    • v.32 no.1
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    • pp.33-40
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    • 1995
  • A space Y is called finitely dominated if there is a finite complex K such that Y is a retract of K in the homotopy category, i.e., we require maps $i : Y \longrightarrow K and r : K \longrightarrow Y with r \circ i \simeq idy$. The following questions are very classical in topology.

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Studies on the Interspecific and Intergeneric Hybridization in Herbage Grasses I. Effects of hybrid embryo age on callus formation and plant regeneration (화본과목초의 종.속간 잡종에 관한 연구 I. 교잡* 일령에 따른 Callus형성과 식물체재분화)

  • 박병훈;김명환
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.9 no.2
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    • pp.62-67
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    • 1989
  • A breeding program in progress at Suweon Livestock Experiment Station, RDA, involves the hybridization of italian ryegrass (Lm) and several leading forage grasses in an effort to combine the nutritive, productivity and palatability qualities of Lm with the adaptive and cold resistance qual; .les of several leading grasses. In order to study the fate of the hybridization between remotely related species, immatured hybrid embryos were cultured on media. The emasculated plants of Lm were Sikem and Tetrone. Reweille, 2n=14, of perennial ryegrass (Lp.), Forager, 2n=42, of tall fescue(Fa), First, 2n=14,of meadow fescue(Fp), Potomac, 2n=28, of orchardgrass(Dg), and Richmond, 2n=42, of timothy(Ph.p) were used as pollinators. Embryos were isolated on 4, 8, 12, 16, and 20 days after pollination and cultured them on modified Murashige and Skoog media. Calluses and plantlets have been obtained after 8 days old embryos crossing between $Lm{\times}Fa$, after 12 days embryos crossing between $Lm{\times}Lp$, Dg, and Ph.p, and after 16 days embryos crossing between $Lm{\times}Fp$. Both callus and shoot formation occurred on 6 % or less of the plated embryos of $Lm{\times}Fp$, Dg, and Ph.p. Embryoderived callus forming shoots have been obtained from 4 days old embryo crossing between $Lm{\times}Dg$ which has not successfully been done anywhere as far as we know. It means that hybrid plants of species without crossability can be obtained through the use of immatured embryo culture. Some of plated embryos developed directly shoots from embryos and the others shoot-forming callus. Cross between related species showed a high frequency of directly shoot formation from plated embryos and cross between remotely related species a high frequency of callus formation.

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RINGS AND MODULES CHARACTERIZED BY OPPOSITES OF FP-INJECTIVITY

  • Buyukasik, EngIn;Kafkas-DemIrcI, GIzem
    • Bulletin of the Korean Mathematical Society
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    • v.56 no.2
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    • pp.439-450
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    • 2019
  • Let R be a ring with unity. Given modules $M_R$ and $_RN$, $M_R$ is said to be absolutely $_RN$-pure if $M{\otimes}N{\rightarrow}L{\otimes}N$ is a monomorphism for every extension $L_R$ of $M_R$. For a module $M_R$, the subpurity domain of $M_R$ is defined to be the collection of all modules $_RN$ such that $M_R$ is absolutely $_RN$-pure. Clearly $M_R$ is absolutely $_RF$-pure for every flat module $_RF$, and that $M_R$ is FP-injective if the subpurity domain of M is the entire class of left modules. As an opposite of FP-injective modules, $M_R$ is said to be a test for flatness by subpurity (or t.f.b.s. for short) if its subpurity domain is as small as possible, namely, consisting of exactly the flat left modules. Every ring has a right t.f.b.s. module. $R_R$ is t.f.b.s. and every finitely generated right ideal is finitely presented if and only if R is right semihereditary. A domain R is $Pr{\ddot{u}}fer$ if and only if R is t.f.b.s. The rings whose simple right modules are t.f.b.s. or injective are completely characterized. Some necessary conditions for the rings whose right modules are t.f.b.s. or injective are obtained.

An Evaluation on Electrochemical Polarization Characteristics and Material Degradation for Cr-Mo-V Steel (Cr-Mo-V강의 전기화학적 분극 특성과 재질열화 평가)

  • Kwon, Il-Hyun;Lee, Song-In;Ha, Jeong-Soo;Yu, Hyo-Sun
    • Journal of the Korean Society for Nondestructive Testing
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    • v.22 no.3
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    • pp.267-274
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    • 2002
  • This research is described on the applicability of a electrochemical techniques for evaluating nondestructive material degradation with various polarization characteristics for Cr-Mo-V steel. The applied electrochemical technique is anodic polarization test which are widely used to evaluate the corrosion rate and/or sensitization at depleted zone of strengthening elements mainly caused by thermal experience for stainless steels. The evaluation of material degradation is performed by small punch test which has been well known as micromechanics test method using specimen size of $10{\times}10{\times}0.5mm$. The 1,000hrs aged material at $630^{\circ}C$ shows the highest material degradation$({\Delta}[DBTT]_{SP})$, but the 2,000hrs and 3,000hrs aged materials show the decrease of ${\Delta}[DBTT]_{SP}$ as aging time increases. It is observed that the difference of current density $({\Delta}I_{FP}\;and\;{\Delta}I_{SP})$.

DISCOVERY TEMPORAL FREQUENT PATTERNS USING TFP-TREE

  • Jin Long;Lee Yongmi;Seo Sungbo;Ryu Keun Ho
    • Proceedings of the KSRS Conference
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    • 2005.10a
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    • pp.454-457
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    • 2005
  • Mining frequent patterns in transaction databases, time-series databases, and many other kinds of databases has been studied popularly in data mining research. Most of the previous studies adopt an Apriori-like candidate set generation-and-test approach. However, candidate set generation is still costly, especially when there exist prolific patterns and/or long patterns. And calendar based on temporal association rules proposes the discovery of association rules along with their temporal patterns in terms of calendar schemas, but this approach is also adopt an Apriori-like candidate set generation. In this paper, we propose an efficient temporal frequent pattern mining using TFP-tree (Temporal Frequent Pattern tree). This approach has three advantages: (1) this method separates many partitions by according to maximum size domain and only scans the transaction once for reducing the I/O cost. (2) This method maintains all of transactions using FP-trees. (3) We only have the FP-trees of I-star pattern and other star pattern nodes only link them step by step for efficient mining and the saving memory. Our performance study shows that the TFP-tree is efficient and scalable for mining, and is about an order of magnitude faster than the Apriori algorithm and also faster than calendar based on temporal frequent pattern mining methods.

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