• Title/Summary/Keyword: hydrophila

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Molecular detection of Aeromonas hydrophila isolated from albino catfish, Clarias sp. reared in an indoor commercial aquarium

  • Choresca, Casiano H. Jr.;Gomez, Dennis K.;Han, Jee-Eun;Shin, Sang-Phil;Kim, Ji-Hyung;Jun, Jin-Woo;Park, Se-Chang
    • Korean Journal of Veterinary Research
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    • v.50 no.4
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    • pp.331-333
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    • 2010
  • Moribund albino catfish, Clarias sp., displayed from an indoor private commercial aquarium were submitted in the laboratory for diagnostic examination. Dense culture of bacteria was recovered from the kidney and was characterized using Vitek System 2 and showed 98% probability to Aeromonas (A.) hydrophila. PCR result showed positive using A. hydrophila extracellular hemolysin gene ahh1 (130 bp) and aerolysin gene aerA (309 bp). The 16S rRNA gene was identical and exhibited 97% sequence similarity with the other known isolates of A. hydrophila available in the GenBank. In this paper, we reported the isolation and molecular detection of A. hydrophila from an albino catfish.

Isolation, Characterization, and Metabolic Profiling of Ceratorhiza hydrophila from the Aquatic Plant Myriophyllum spicatum

  • Elsaba, Yasmin M.;Boroujerdi, Arezue;Abdelsalam, Asmaa
    • Mycobiology
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    • v.50 no.2
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    • pp.110-120
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    • 2022
  • The goal of the present study was to investigate the antibacterial properties, enzyme production, and metabolic profiling of a new Ceratorhiza hydrophila strain isolated from the submerged aquatic plant Myriophyllum spicatum. Furthermore, the fungus' morphological characterization and DNA sequencing have been described. The fungus has been identified and submitted to the GenBank as Ceratorhiza hydrophila isolate EG19 and the fungus ID is MK387081. The enzyme analyses showed its ability to produce protease and cellulase enzymes. According to the CSLI standard, the ethyl acetate extract of C. hydrophila showed intermediate antibacterial activity against Streptococcus pneumonia, Micrococcus luteus, and Staphylococcus aureus. Metabolic profiling has been carried out using 700 MHz NMR spectroscopy. Based on the 1H and 1H-13C heteronuclear single quantum coherence (HSQC) NMR data and NMR databases, 23 compounds have been identified. The identified metabolites include 31% amino acids, 9% sugars, 9% amines, 4% sugar alcohols, and 4% alkaloids. This is the first report for the metabolic characterization of C. hydrophila, which gave preliminary information about the fungus. It is expected that our findings not only will pave the way to other perspectives in enormous applications using C. hydrophila as a new promising source of antimicrobial agents and essential metabolites, but also it will be valuable in the classification and chemotaxonomy of the species.

Characterization of extracellular proteases of Aeromonas hydrophila isolated from the intestine of carp(Cyprinus carpio) (잉어(Cyprinus carpio)로부터 분리된 Aeromonas hydrophila의 extracelluar proteases 연구)

  • Lee, Jong-Kyu;Kim, Jong-Pil;Choi, Tae-Jin;Song, Young-Hwan
    • Journal of fish pathology
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    • v.10 no.1
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    • pp.31-38
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    • 1997
  • Aeromonas hydrophila isolated from the intestine of carp produced several kinds of proteases into the medium. Inhibitor assay with the culture supernatant of A. hydrophila showed that there were major metalloproteases and minor serine proteases. Gelatin SDS-PAGE showed two proteolytic bands. One broad protease band was inhibited by metalloprotease specific inhibitor, EDTA, indicating a metalloprotease. The other was inhibited by serine protease specific inhibitor, PMSF, suggesting a serine protease. The proteolytic activities of both extracellular proteases remained on Gelatin SDS-PAGE after heating at $70^{\circ}C$ for 30 min. However, the major metalloprotease was separated into two proteolytic bands on Gelatin PAGE by gel filtration chromatography on Sephadex G-75.

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Isolation and Cultural Characteristics of a Phosphate-Solubilizing Bacterium, Aeromonas hydrophila DA57 (인산가용화균 Aeromonas hydrophila DA 57의 분리와 배양 중 가용화특성)

  • Song, Ok-Ryul;Lee, Seung-Jin;Kim, Se-Hoon;Chung, Soo-Yeol;Cha, In-Ho;Choi, Yong-Lark
    • Applied Biological Chemistry
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    • v.44 no.4
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    • pp.251-256
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    • 2001
  • To develop biofertilizer solubilizing inorganic phosphate, a bacterium having high abilities to solubilize inorganic phosphate were isolated from cultivated soils. The strain was identified to Aeromonas hydrophila DA57, based on the physiological and biochemical properties. The optimum temperature and initial pH to solubilize insoluvle phosphate in sucrose minimal medium were $30^{\circ}C$ and pH 7.0, respectively. In these conditions phosphate solubilizing activities of the strain against three types of insoluble phosphate were quantitatively determined. It was possivle to distinguish between solubilization through release of gluconic acid and still unknown mechanism. Aemmonas hydrophila DA57 harbored a 4.5 kb cryptic plasmid.

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Modifications Induced by Organic Solvents to Aeromonas hydrophila IB Bct4 (유기용매에 의하여 유발된 Aeromonas hydrophila Bct4의 변형)

  • Lazaroaie, Mihaela Marilena
    • Journal of the Korean Chemical Society
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    • v.53 no.3
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    • pp.325-334
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    • 2009
  • Many bacteria that are capable to tolerate and degrade organic solvents have been isolated from seawater. However, their roles in the biodegradation of organic solvents in the marine environment have remained unknown. Aeromonas hydrophila IB$B_{ct4}$, isolated from Constanta seawater, was able to tolerate and degrade different organic solvents. Toluene, styrene, xylene isomers, ethylbenzene, with the logarithm of the partition coefficient in octanol-water mixture (log $P_{ow}$) between 2.64 and 3.17, were more toxic for bacterial cells, compared with propylbenzene, n-hexane, n-heptane, with log $P_{ow}$ between 3.69 and 4.39. There were revealed cellular and molecular modifications induced by organic solvents to Aeromonas hydrophila IB$B_{ct4}$. The study of cellular and molecular modifications induced by different organic solvents showed a complex response of bacterial cells to the presence of organic solvents in the culture media.

Isolation and Characterization of Aeromons hydrophila PBl6 and Properties of Synthetic Wastewater Degradation (Protease 생성균 Aeromonas hydrophila PB16의 분리 및 합성폐수처리능)

  • 박형수;양선영;김무훈;이종광;유용호;박두현
    • Korean Journal of Microbiology
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    • v.38 no.4
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    • pp.235-240
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    • 2002
  • Protease producing bacterium, PB16 was isolated from food processing wastewater sludge and paddy field soil samples and selected by the clear zone and enzyme activity test. The isolate was gram negative, rod type and its protease productivity was 6.49 U/ml. As a result of API20NE kit test and 16S rDNA sequencying, the isolated PB16 was identified as Aeromonas hydrophila (99%). The growth rate ($h^{-1}$) was 0.21 in synthetic waste water only and 0.26 in synthetic waste water containing vitamin and mineral using a bioscreen C. Synthetic wastewater removal rate was 59 and 87%, respectively after 1 and 3 day reaction (intial CODcr was 2,472 mg/l).

Molecular Cloning, Purification, and Characterization of an Extracellular Nuclease from Aeromonas hydrophila ATCC14715

  • Nam, In-Young;Myung, Hee-Joon;Joh, Ki-Seong
    • Journal of Microbiology and Biotechnology
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    • v.14 no.1
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    • pp.178-181
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    • 2004
  • A gene encoding an extracellular nuclease was cloned from Aeromonas hydrophila strain ATCC14715. The gene was overexpressed and the enzyme was purified by fusing to maltose binding protein. It was shown that the protein possessed DNase activity on both single-stranded and double-stranded DNAs. It exhibited both endo- and exonuclease activities. It was also shown that the protein had an RNase activity. Possible roles of this extracellular enzyme in the A. hydrophila life cycle are discussed.

A Case of Peritoneal Dialysis-related Peritonitis Caused by Aeromonas Hydrophila in the Patient Receiving Automated Peritoneal Dialysis

  • Kim, Hyun Jin;Park, Hyun Sun;Bae, Eunsin;Kim, Hae Won;Kim, Beom;Moon, Kyoung Hyoub;Lee, Dong-Young
    • Electrolytes & blood pressure
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    • v.16 no.2
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    • pp.27-29
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    • 2018
  • Peritoneal dialysis (PD)-related peritonitis is a major cause of injury and technique failure in patients undergoing PD. Aeromonas hydrophila is ubiquitous in the environment, and is a Gram-negative rod associated with infections in fish and amphibians in most cases; however, it can also cause opportunistic infections in immunocompromised patients. We report a case of A. hydrophila peritonitis in a 56-year-old male on automated PD. Peritonitis may have been caused by contamination of the Set Plus, a component of the automated peritoneal dialysis device. Although Set Plus is disposable, the patient reused the product by cleansing with tap water. He was successfully treated with intraperitoneally-administered ceftazidime and has been well without recurrence for more than 2 years.

Effect of Sodium Hypochlorite on the Biofilms of Aeromonas hydrophila, Streptococcus mutans, and Yersinia enterocolitica

  • Youngseok Ham;Han-Saem Park;Minjun Kim;Tae-Jong Kim
    • Microbiology and Biotechnology Letters
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    • v.51 no.1
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    • pp.32-36
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    • 2023
  • In this study, the effect of sodium hypochlorite on biofilm removal was evaluated using three bacterial strains; Aeromonas hydrophila, Streptococcus mutans, and Yersinia enterocolitica. For maximum biofilm removal in 10 min, sodium hypochlorite is required at 1.65, 0.83, and 0.41 g/l for A. hydrophila, S. mutans, and Y. enterocolitica, respectively. Resistance to sodium hypochlorite was increased by the biofilms of all three tested strains, while the change in bactericidal activity according to sodium hypochlorite concentration was strain-specific. Therefore, we aimed to determine the effective concentration of sodium hypochlorite required for hygiene, considering that higher concentrations are needed to remove biofilms than to kill cells.

Two Unrecorded Species, Globodera hypolysi and Sarisodera hydrophiza (Nematoda: Heteroderidae) from Korea (씨스트선충과 (Nematoda: Heteroderidae)의 한국 2 미기록종 Globodera hypolysi와 Sarisodera hydrophila의 기재)

  • Park, Young-Eoun;Kim, Sae-Eon
    • Korean journal of applied entomology
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    • v.40 no.1
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    • pp.5-13
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    • 2001
  • Two unrecorded species of Heteroderinae, Clobodera hypolysi Ogawa, Ohshima and Ichinohe, 1983 and Sarisodera hydrophila Wouts and Sher, 1971 from Korea were firstly reported. The Korean specimens well correspond with the original description of Globodera hypolysi except stylet length of the juveniles, slightly shorter (21~24 vs. 24~25 $\mu$m) and shorter distance from the anus to the fenestra in cyst (22~50 vs. 25~80$\mu$m). The measurements of Korean specimen of Sarisodera hydrophila are slightly short compared with the type species; Female body length 367.2~597.6$\mu$m vs 359~688$\mu$m; Stylet length 37~44$\mu$m vs 38~46$\mu$m; Male body length 662~11987m vs 579~1405$\mu$m; stylet length 37~43.8$\mu$m vs 38~46$\mu$m. Juvnile body length 460~536$\mu$m vs 466~5587m. stylet length 32~387m vs 39~43$\mu$m.

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