• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2005년도 제17회 워크샵 및 추계학술대회
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• pp.299-306
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• 2005

Anaerobic fermentation of food waste (FW) and waste activated sludge (WAS) for hydrogen production was performed in CSTR (Continuous Stirred tank reactor) under various HRTs and volumetric mixing ratio (V/V) of two substrates, FW and WAS. The specific hydrogen production potential of FW was higher than that of WAS. However, pH drop in the CSTR for hydrogen production from FW was higher than that from WAS. The maintenance of desired pH during fermentative hydrogen production is regarded as the most important operation parameter for the stable hydrogen production. Therefore, when the potential of hydrogen production from FW and better buffer capacity of WAS, the proper mixture of FW and WAS for fermentative hydrogen production were considered as a useful complementary substrate. The maximum yield of specific hydrogen production, 140 mL/g VSS, was found at HRT of 2 day and the volumetric mixing ratio of 20:80 (WAS : FW). The spatial distribution of hydrogen producing bacteria was observed in anaerobic fermentative reactor using fluorescent in situ hybridization (FISH) method.

• 공업화학
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• 제18권2호
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• pp.162-167
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• 2007

본 연구에서는 Clostridium beijerinckii Donker 1926을 이용한 연속식 혐기성 소화공정에서 수소 생산과 동력학적 특성을 고찰하였다. 기질은 glucose를 사용하였고, 0.5, 0.25, 0.125일의 체류시간 (hydraulic retention time, HRT)에서 실험이 이루어졌으며, 모든 HRT의 조건에서 탄산화물은 99% 이상의 제거효율를 나타내었다. 체류시간이 짧을수록, COD 제거율은 낮은 반면에, 전체 가스 중에서 수소 가스 함량과 수소 발생량이 높게 나타났다. 또한, 정상상태에서, 증식 수율과 수소가스 생성 수율은 각각 0.27 g-VSS/g- glucose, 0.26 L/g-glucose로 나타났다. 본 실험에 사용된 균주를 glucose와 같이 당 성분이 함유된 폐수처리에 적용하면 수소를 생산할 수 있으며, 이러한 실험 결과들을 잘 활용하면 대체에너지로서, 실제적인 수소가스 생산 시스템에 적용할 수 있을 것이다.

• 한국수소및신에너지학회논문집
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• 제23권4호
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• pp.397-403
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• 2012

Galactose, an isomer of glucose with an opposite hydroxyl group at the 4-carbon, is a major fermentable sugar in various promising feedstock for hydrogen production including red algal biomass. In this study, hydrogen production characteristics of galactose-glucose mixture were investigated using batch fermentation experiments with heat-treated digester sludge as inoclua. Galactose showed a hydogen yield compatible with glucose. However, more complicated metabolic steps for galactose utilization caused a slower hydrogen production rate. The existence of glucose aggravated the hydrogen production rate, which would result from the regulation of galactose-utilizing enzymes by glucose. Hydrogen produciton rate at galactose to glucose ratio of 8:2 or 6:4 was 67% of the production rate for galactose and 33% for glucose, which could need approximately 1.5 and 3 times longer hydraulic retention time than galacgtose only condition and glucose only condition, respectively, in continuous fermentation. Hydrogen production rate, Hydrogen yield, and organic acid production at galactose to glucose ratio of 8:2 or 6:4 were 0.14 mL H2/mL/hr, 0.78 mol $H_2$/mol sugar, and 11.89 g COD/L, respectively. Galactose-rich biomass could be usable for hydogen fermenation, however, the fermentation time should be allowed enough.

• 한국수소및신에너지학회논문집
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• 제24권2호
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• pp.99-106
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• 2013

The variations of performance and metabolites at an early stage were investigated for the successful start-up technology in continuous fermentative hydrogen production. Unsuccessful start-up was observed when the operation mode was changed from batch to continuous mode after the yield was reached to 0.5 mol $H_2$/mol $hexose_{added}$ by batch mode. $H_2$ production continued till 12 hours accompanied by butyrate production, but did not last with propionate production increase. It was suspected that the failure was due to the regrowth of propionic acid bacteria during batch mode which were inhibited by heat-shock but not completely killed. Thus, successful start-up was tried by early switchover from batch to continuous operation; continuous operation was started after the $H_2$ yield was reached to 0.2 mol $H_2$/mol $hexose_{added}$ by batch mode. Although $H_2$ production rate decreased at an early stage, stable $H_2$ yield of 0.8 mol $H_2$/mol $hexose_{added}$ was achieved after 10 days by lowering down propionate production. And it was also concluded that the reason for $H_2$ production decrease at an early stage was due to alcohol production by self detoxification mechanism against VFAs accumulation.

• 한국환경보건학회지
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• 제30권2호
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• pp.149-153
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• 2004

연속반응조에서의 수소생산에 대한 pH의 영향을 HRT 10시간으로 유지하고, pH 4.1부터 8.0까지의 범위에서 조사하였다. 실험조건에서의 생성된 수소가스 성분은 41-71% 범위로 발생되었다. $H_2$/$CO_2$ 비율은 pH 6.0 이상에서는 크게 변화가 없었으나, 대체적으로 pH가 증가함에 따라 $H_2$/$CO_2$ 비율도 증가하였다. 최대 수소생성수율은 pH 5.0에서 3.16$\ell$/g sucrose이었다. Acetate 생성은 pH 증가에 따라 증가하였으나, butyrate 생성은 pH 증가에 따라 감소하였다. 미생물량은 pH 증가에 따라 증가하였다.

• Journal of Microbiology and Biotechnology
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• 제24권11호
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• pp.1542-1550
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• 2014

Co-digesting molasses wastewater and sewage sludge was evaluated for hydrogen production by response surface methodology (RSM). Batch experiments in accordance with various dilution ratios (40- to 5-fold) and waste mixing composition ratios (100:0, 80:20, 60:40, 40:60, 20:80, and 0:100, on a volume basis) were conducted. Volatile solid (VS) concentration strongly affected the hydrogen production rate and yield compared with the waste mixing ratio. The specific hydrogen production rate was predicted to be optimal when the VS concentration ranged from 10 to 12 g/l at all the mixing ratios of molasses wastewater and sewage sludge. A hydrogen yield of over 50 ml $H_2/gVS_{removed}$ was obtained from mixed waste of 10% sewage sludge and 10 g/l VS (about 10-fold dilution ratio). The optimal chemical oxygen demand/total nitrogen ratio for co-digesting molasses wastewater and sewage sludge was between 250 and 300 with a hydrogen yield above 20 ml $H_2/gVS_{removed}$.

• 한국수소및신에너지학회논문집
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• 제34권1호
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• pp.1-7
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• 2023

A fuel handling process combined with a pressure swing adsorption system (PSA) was simulated to produce pure hydrogen with a purity greater than 99.97%. The simulation consists of two parts. The fuel processing part consisting of reformer and water-gas shift reaction was simulated with Aspen plus^®, and the hydrogen purification part consisting of PSA was simulated with Aspen Adsorption^®. In this study, the effect of reformer temperature and pressure on the total hydrogen production yield was investigated. Simulations were performed over a temperature range of 700 to 1,000℃ and a pressure range of 1 to 10 bar. The total hydrogen production yield increased with increasing temperature and decreasing pressure. The maximum hydrogen yield was less than 50% in the simulation and will be lower in the real process.

• Nuclear Engineering and Technology
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• 제51권1호
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• pp.214-220
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• 2019

Thermochemical cycles have been predominantly used for energy transformation from heat to stored chemical free energy in the form of hydrogen. The thermochemical cycle based on uranium (UTC), proposed by Oak Ridge National Laboratory, has been considered as a better alternative compared to other thermochemical cycles mainly due to its safety and high efficiency. UTC process includes three steps, in which only the first step is unique. Hydrogen production apparatus with hectogram reactants was designed in this study. The results showed that high yield hydrogen was obtained, which was determined by drainage method. The results also indicated that the chemical conversion rate of hydrogen production was in direct proportion to the mass of $Na_2CO_3$, while the solid product was $Na_2UO_4$, instead of $Na_2U_2O_7$. Nevertheless the thermochemical cycle used for hydrogen generation can be closed, and chemical compounds used in these processes can also be recycled. So the cycle with $Na_2UO_4$ as its first reaction product has an advantage over the proposed UTC process, attributed to the fast reaction rate and high hydrogen yield in the first reaction step.

• 한국수소및신에너지학회논문집
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• 제17권4호
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• pp.362-370
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• 2006

The purpose of this paper was to investigate the reforming characteristics and optimum operating condition of the plasmatron assisted $CH_4$ reforming reaction for the hydrogen-rich gas production. Also, in order to increase the hydrogen production and the methane conversion rate, parametric screening studies were conducted, in which there were the variations of the $CH_4$ flow ratio, $CO_2$ flow ratio, vapor flow ratio, mixing flow ratio and catalyst addition in reactor. High temperature plasma flame was generated by air and arc discharge. The air flow rate and input electric power were fixed 5.1 l/min and 6.4 kW, respectively. When the $CH_4$ flow ratio was 38.5%, the production of hydrogen was maximized and optimal methane conversion rate was 99.2%. Under these optimal conditions, the following synthesis gas concentrations were determined: $H_2$, 45.4%; CO, 6.9%; $CO_2$, 1.5%; and $C_2H_2$, 1.1%. The $H_2/CO$ ratio was 6.6, hydrogen yield was 78.8% and energy conversion rate was 63.6%.

• Advances in Energy Research
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• 제2권1호
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• pp.1-9
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• 2014

The green microalgae Chlamydomonas reinhardtti is well-known specie in the terms of $H_2$ production by photo fermentation and has been studying for a long time. Although the $H_2$ production yield is promising; there are some bottlenecks to enhance the yield and efficiency to focus on a well-designed, sustainable production and also scaling up for further studies. D1 protein of photosystem II (PSII) plays an important role in photosystem damage repair and related to $H_2$ production. Because Chlamydomonas is the model algae and the genetic basis is well-studied; metabolic engineering tools are intended to use for enhanced production. Mutations are focused on D1 protein which aims long-lasting hydrogen production by blocking the PSII repair system thus $O_2$ sensitive hydrogenases catalysis hydrogen production for a longer period of time under anaerobic and sulfur deprived conditions. Chlamydomonas CC124 as control strain and D1 mutant strains(D240, D239-40 and D240-41)are cultured photomixotrophically at $80{\mu}mol\;photons\;m^{-2}s^{-1}$, by two sides. Cells are grown in TAP medium as aerobic stage for culture growth; in logarithmic phase cells are transferred from aerobic to an anaerobic and sulfur deprived TAP- S medium and 12 mg/L initial chlorophyll content for $H_2$ production which is monitored by the water columns and later detected by Gas Chromatography. Total produced hydrogen was $82{\pm}10$, $180{\pm}20$, $196{\pm}20$, $290{\pm}30mL$ for CC124, D240, D239-40, D240-41, respectively. $H_2$ production rates for mutant strains was $1.3{\pm}0.5mL/L.h$ meanwhile CC124 showed 2-3 fold lower rate as $0.57{\pm}0.2mL/L.h$. Hydrogen production period was $5{\pm}2days$ for CC124 and mutants showed a longer production time for $9{\pm}2days$. It is seen from the results that $H_2$ productions for mutant strains have a significant effect in terms of productivity, yield and production time.