• Journal of Microbiology
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• v.35 no.4
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• pp.264-270
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• 1997

The structure of plasmid mlp1, a linear 10.2kb mitochondrial plasmid of Pleurotus ostreatus NFF A2 was determined by restriction enzyme mapping and partial sequencing. The plasmid encodes at least two proteins; a putative RNA polymerase showing homology to yeast mitochondrial RNA polymerase and to viral-encoded RNA polymerases, and a putative DNA polymerase showing significant homology to the family B thpe DNA polymerases. It also contains terminal inverted repeat sequences at both ends which are longer than 274 bp. A 1.6 kb EcoRI restriction fragment of m1p1 containing the putative RNA polymerase gene did not hybridize to the nuclear or motochondrial genomes from P. ostreatus, suggesting that it may encode plasmidspecific RNA polymerase. The gene fragment also did not hybridize with the RNA polymerase gene (RPO41) from Saccaromyces cerevisiae. The relationship between genes in m1p1 and those in another linear plasmid pC1K1 of Claviceps purpurea was examined by DNA hybridization. The result indicates that the genes for DNA and RNA polymerases are not closely related with those in C. purpurea.

• Journal of Sericultural and Entomological Science
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• no.12
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• pp.17-20
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• 1970

Occasionally, We make mistake of discrimination of sex since a pair of small spots around tile abdomen of 9th abdominal of males and posterior spots of females of the silkworms larvae are found at almost same position. As already have reported, more larvae which have the small spots were found in Chinese race than the Japanese. This research has been conducted in order to grasp the rate of appearance of the small spotted larvae hereditarily when hybridize with different race (F$_1$) 1. According to the first report, average 17,8％ of the small spotted larvae are found in the Japanese race and 57.0％ in the Chinese, but my re-invested data which was carried out recently shows that average 13.6％ in the former case and 37.8％ in the latter. Those new data are supposed to be come to a conclusion that the figures are flexible but in any case high frequency of appearance of the larvae were found in the Chinese race than the Japanese (Reference Table 1 ) 2. Miscalculated ratio of the larvae from males to females are 1.2-2.6％, and from females to males are found 0.5％-0.9％ in the Japanese race, In case of the Chinese race, 7.1-9.4％ in tile former and 2.9-3.4％ in the latter. Those data also shows that more of the larvae found in Chinese race. 3. The larvae of small spotted and non-spotted are also seperated when hlybridize with different mating types and races. 4. More small spotted larvae are seen when hybridize with less appeared of the small spots of females and males which have the small spots than more appeared of the small spots of females with none spotted of the males (Reference Table 2. C.G.)B.F.). 5. More larvae of the small spots and found in case of hybridize in a group of having small spots than none of the spots (H>E). 6. With the exception of the case (A.B), in the same type of cross breeding (C.D, E.F, G,H), more larvae of the smal1 spots are also found in case of hybridize with males of having the spots than none of the spots.

• Proceedings of the Korean Vacuum Society Conference
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• 2015.08a
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• pp.228.1-228.1
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• 2015

We demonstrate a simple route to hybridize two different nanomaterials by using three-dimensional nanodishes that can be used as small plasmonic containers to host guest nanoparticles. Our nanodishes were fabricated using nanoimprint lithography and oblique-angle film deposition, and the guest nanoparticles were drop-casted onto the host nanodishes. Based on the proposed method, colloidal Au nanoparticles were assembled inside Au nanodishes in the form of a labyrinth. These Au nanoparticle-nanodish hybrids excited a strong surface plasmon resonance, as verified by a numerical simulation of the local field enhancement and by direct observation of the enhanced Raman signals. Our results point to the potential of the nanodishes as a useful platform for combining diverse nanomaterials and their functionalities.

• The Journal of Art Theory & Practice
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• no.10
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• pp.105-118
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• 2010

This study discusses the community of participants in new media-based art of Taeyoon Choi, Wafaa Bilal and Mushon Zer-Aviv in relation to current discourses on social functions of art by Nicolas Bourriaud and Jacques Ranciere. Focusing on these artists' participatory projects which aim to provide alternative perspectives on wars between countries, to raise awareness about expanding surveillance systems in city spaces, or to create new public spaces on the web, this paper argues that their works hybridize entertainment culture and political activism to suggest a new model for political art.

• The Journal of Art Theory & Practice
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• no.10
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• pp.63-84
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• 2010

This essay examines how the figure of Liberty has been refashioned in the streets of post-apartheid South Africa, addressing three public art works installed in Johannesburg over the past decade: Reshada Crouse's oil painting Passive Resistance, Marlene Dumas' tapestry The Benefit of the Doubt and William Kentridge's and Gerhard Marx's sculpture Firewalker. Even as these monumental works all reprise Delacroix's Liberty on the Barricades-an icon of the city street and its revolutionary barricades-so too this trio of Liberties have become mere phantoms of their vaunted archetype. Haunted specters, they quarrel with the mythologized chimera of Liberty, taking issue with the fraught tradition of pinning regime change onto the body of the female nude. Drawing instead on South African histories of women's resistance, in which female nudity has been repeatedly marshaled as a form of dissent, the Liberties circling Johannesburg hybridize their European template with local traditions of female political opposition to colonial and postcolonial male authority.

• Biotechnology and Bioprocess Engineering:BBE
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• v.8 no.4
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• pp.263-267
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• 2003

Magnetic nanoparticles prepared from an alkaline solution of divalent and trivalent iron ions could covalently bind protein via the activation of Nethyl-N-(3-dimethylaminopropyl) carbodiimide (EDC). Trypsin and avidin were taken as the model proteins for the formation of protein-nanoparticle conjugates. The immobilized yield of protein increased with molar ratio of EDC/nanoparticie. Higher concentrations of added protein could yield higher immobilized protein densities on the particles. In contrast to EDC, the yields of protein immobilization via the a ctivation of cyanamide were relatively lower. Nanoparticles bound with avidin could attach a single-stranded DNA through the avidin-biotin interaction and hybridize with a DNA probe. The DNA hybridization was confirmed by fluorescence microscopy observations. Immobilized DNA on nanoparticles by this technique may have widespread applicability to the detection of specific nucleic acid sequence and targeting of DNA to particular cells.

• Journal of the Korean Society for Industrial and Applied Mathematics
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• v.17 no.4
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• pp.279-294
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• 2013

We are interested in an adaptive grid method for hyperbolic equations. A multiresolution analysis, based on a biorthogonal family of interpolating scaling functions and lifted interpolating wavelets, is used to dynamically adapt grid points according to the physical field profile in each time step. Traditional finite-difference schemes with fixed stencils produce high oscillations around sharp discontinuities. In this paper, we hybridize high-resolution schemes, which are suitable for capturing singularities, and apply a finite-difference approach to the scaling functions at non-singular points. We use a total variation diminishing Runge-Kutta method for the time integration. The computational cost is proportional to the number of points present after compression. We provide several numerical examples to verify our approach.

• Journal of the Korean Society of Tobacco Science
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• v.17 no.2
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• pp.109-113
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• 1995

Nicotiana repanda has resistance to many important tobacco diseases but no tobacco cultivars are currently available that carry risistance genes derived from this species. Numerous attempts to hybridize N, repanda with commercial tobacco cultivars have been largely unsuccessful because of cross incompatibility or the uncovering of lethal genes. In vitro pollination of placenta attached ovules was useful in by passing prezygotic barriers for interspecific hybrid combination between N.tabacum cv. NC82 and N. repanda. Six days after in vitro pollination of N. tabacum cv. NC82×N. repanda, enlarged ovules on plancenta were removed and transferred into ovule culture medium of kitsch and kitsch (1969). Within 15 days of ovule culture, germination occurred. Most of the hybrid seedlings obtained had poor root system and finally died, while few of them had good root system and grew well.

• Journal of Microbiology and Biotechnology
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• v.18 no.11
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• pp.1858-1861
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• 2008

A PCR-enzyme-linked immunosorbent assay (PCR-ELISA) was developed for the rapid and sensitive detection of L. monocytogenes. PCR primers generating a 132-bp amplicon and a capture probe able to hybridize to the PCR amplicon were designed based on the L. monocytogenes-specific hly gene encoding listeriolysin. The detection limit of PCR-ELISA for L. monocytogenes was determined to be as low as 10 cells per PCR reaction, and this level of detection was achieved within 5 h. These results indicate that the PCR-ELISA provides a valuable tool for the rapid and sensitive detection of L. monocytogenes for the ready-to-eat food industry.

• Archives of design research
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• v.18 no.4 s.62
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• pp.185-194
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• 2005

In the perspective of hybrid, design contains confused forms and styles. Thus, the question is what 'decisively affects' on life, happiness and peace of human beings: In the past scientific ecology that tries to solve the problem in the aspect of functional design was important. Now the question is beyond the scientific ecology because human has a different way of environmental adaptation from animals. From this point of view, design is defined as something beyond materialistic standard. Terry Eagleton said, 'Creative ability deviating oneself and transcending is the very standard to measure human beings' A tendency to hybridize one's own section off genre or pursuing dismantlement is found on such creative perspective. In other words, it focuses on making a space of human's cultural behavior by unifying creatively natural environment and architecture space in the organic aspect. Such phenomenon provides a new chance and possibility in environmental design through a new molding experiment according to the development of digital technology. As experiments of environment design using digital technology are actively implemented, ecological and cultural views hybridize to create a new cultural space of various functions and features. Such experiment in environment design highlights the connections of ecological view, recognition of value, and relation-centered ideas, and enables a tool pursuing a new cultural development. This study focuses on the paradigm of such digital hybrid design and purposes to set the system by analyzing the application styles and principles of environment design through theoretical and case studies. In addition, it promotes a new possibility to develop environment design in the history of civilization.