• Molecules and Cells
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• v.22 no.2
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• pp.210-219
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• 2006

Dynamin-related protein 2A (AtDRP2A, formally ADL6), a member of the dynamin family, is critical for protein trafficking from the TGN to the central vacuole. However, the mechanism controlling its activity is not well understood in plant cells. We isolated Arabidopsis sec13 homolog1 (AtSeh1) that interacts with AtDRP2A by a yeast two-hybrid screening. AtSeh1 has four WD40 motifs and amino acid sequence homology to Sec13, a component of COPII vesicles. Coimmunoprecipitation and protein pull-down experiments demonstrated specific interaction between AtSeh1 and AtDRP2A. AtSeh1 bound to the pleckstrin homology domain of AtDRP2A in competition with the C-terminal domain of the latter, and this resulted in inhibition of the interaction between AtDRP2A and PtdIns3P in vitro. AtSeh1 localized to multiple locations: the nucleus, the prevacuolar compartment and the Golgi complex. Based on these results we propose that AtSeh1 plays a role in regulating cycling of AtDRP2A between membrane-bound and soluble forms.

• The Korean Journal of Zoology
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• v.31 no.1
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• pp.56-61
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• 1988

The taxonomic status of Moroco Jagowskii and M oxycephalus a pair of sibling species inhabiting in Korean fresh waters, has been unclear up to date. Recently a sympatric area of these species was found (Kang, 1987). The purpose of this study was to clarify their specific status by analysing specimens collected from the sympatric area of these species. Isozyme analysis and morphometric comparison were performed. Among 26 loci screened 6 loci (Aco, Est-2, E-X, Gk-3, Ipo, Me) showed fixed allelic difference between them and these loci could be used as genetic markers to distinguish them. Isozyme analysis indicates that no hybridization occurs and therefore it is assumed that isolating mechanism is completed and they are distinct species. The mean number of scales above lateral line (SAL) of M Jagowskii and M oxycephalus at sympatric area was 24.93 $\pm$1.95 and 17.33$\pm$0.72 respectively, and it seems as the result of character displacement. A finer microhabitat segregation between them is noticed. M oxycephalus is found along the effluent streams whereas M lagowskii is distributed mostly in the main stream at sympatric area.

• Molecules and Cells
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• v.41 no.9
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• pp.842-852
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• 2018

Notch signaling is an evolutionarily conserved pathway and involves in the regulation of various cellular and developmental processes. Ligand binding releases the intracellular domain of Notch receptor (NICD), which interacts with DNA-bound CSL [CBF1/Su(H)/Lag-1] to activate transcription of target genes. In the absence of NICD binding, CSL down-regulates target gene expression through the recruitment of various corepressor proteins including SMRT/NCoR (silencing mediator of retinoid and thyroid receptors/nuclear receptor corepressor), SHARP (SMRT/HDAC1-associated repressor protein), and KyoT2. Structural and functional studies revealed the molecular basis of these interactions, in which NICD coactivator and corepressor proteins competitively bind to ${\beta}-trefoil$ domain (BTD) of CSL using a conserved ${\varphi}W{\varphi}P$ motif (${\varphi}$ denotes any hydrophobic residues). To date, there are conflicting ideas regarding the molecular mechanism of SMRT-mediated repression of CSL as to whether CSL-SMRT interaction is direct or indirect (via the bridge factor SHARP). To solve this issue, we mapped the CSL-binding region of SMRT and employed a 'one- plus two-hybrid system' to obtain CSL interaction-defective mutants for this region. We identified the CSL-interaction module of SMRT (CIMS; amino acid 1816-1846) as the molecular determinant of its direct interaction with CSL. Notably, CIMS contains a canonical ${\varphi}W{\varphi}P$ sequence (APIWRP, amino acids 1832-1837) and directly interacts with CSL-BTD in a mode similar to other BTD-binding corepressors. Finally, we showed that CSL-interaction motif, rather than SHARP-interaction motif, of SMRT is involved in transcriptional repression of NICD in a cell-based assay. These results strongly suggest that SMRT participates in CSL-mediated repression via direct binding to CSL.

• Membrane Journal
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• v.22 no.3
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• pp.155-170
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• 2012

Fuel cells have been considered as alternative power generation system in the twenty-first century because of eco-friendly system, high power density and efficiency compare with petroleum engine system. Proton exchange membranes (PEMs) are the key components in fuel cell system. Currently, Nafion has been used in fuel cell system. However, Nafion has disadvantages such as low conductivity at high temperature and high cost. The researchers have focused to reach the high properties such as high proton conductivity, low permeability to fuel, good chemical/thermal stability, good mechanical properties and low manufacturing cost. Various methods have been developed for preparation of proton exchange membrane with high performance and commercialization of fuel cell system. The hybrid organic/inorganic membrane has the potentials to provide a unique combination of organic and inorganic properties with improved proton conductivity and mechanical property at high temperatures. So, this paper presents an overview of research trend for the composite membranes prepared by organic/inorganic system using various inorganic materials.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.42 no.6
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• pp.77-82
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• 2005

This paper is proposed fuzzy neural network(FNN) and artificial neural network(ANN) based on the vector controlled induction motor drive system. The hybrid combination of fuzzy control and neural network will produce a powerful representation flexibility and numerical processing capability. Also, this paper is proposed control and estimation of speed of induction motor using fuzzy and neural network. The back propagation neural network technique is used to provide a real time adaptive estimation of the motor speed. The error between the desired state variable and the actual one is back-propagated to adjust the rotor speed, so that the actual state variable will coincide with the desired one. The back propagation mechanism is easy to derive and the estimated speed tracks precisely the actual motor speed. This paper is proposed the experimental results to verify the effectiveness of the new method.

• Proceedings of the Korea Concrete Institute Conference
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• 2008.04a
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• pp.177-180
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• 2008

SHCC shows the high energy tolerance capacity due to the interfacial bonding of the fibers to the cement matrix. For effective material design and application of SHCC, it is needed to investigate the damage process and micro-fracture mechanism of cement matrix reinforced with different types of fibers. The objective of this paper is to investigate the direct tensile response of cement composites reinforced with single and hybrid fibers using acoustic emission(AE) technique. In this study, the correlations between AE signal and result of the direct tensile response of SHCC. For these purposes, three kinds of fibers were used: PET1.5%, PET1.0+PE0.5%, PET1.0%+PVA0.5%. The result of the direct tensile response of SHCC, for the same volume fraction of fibers, ultimate strength of PET-PE specimen was 2.7 times higher than specimens with PET fibers. And from AE signal value, AE event numbers and cumulative energy were different according to kind of fiber because of the different material properties of reinforced fiber.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.14 no.12
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• pp.2811-2817
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• 2010

In this paper we propose the QAC-MAC that supports Quality of Service(QoS) and saves energy resources of the sensor node, and hence prolonging the lifetime of the sensor network with multiple sink nodes. Generally, the nodes nearest to the sink node often experience heavy congestion since all data is forwarded toward the sink through those nodes. So this critically effects on the delay-constraint data traffics. QAC-MAC uses a hybrid mechanism that adapts scheduled scheme for medium access and scheduling and unscheduled scheme based on TDMA for no data collision transmission. Generally speaking, characteristics of the real-time traffic with higher priority tends to be bursty and has same destination. QAC-MAC adapts cross-layer concept to rearrange the data transmission order in each sensor node's queue, saves energy consumption by allowing few nodes in data transmission, and prolongs the network lifetime.

• BMB Reports
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• v.42 no.8
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• pp.486-492
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• 2009

OsDREB1D, a special DREB (dehydration responsive element binding protein) homologous gene, whose transcripts cannot be detected in rice (Oryza sativa L), either with or without stress treatments, was amplified from the rice genome DNA. The yeast one-hybrid assay revealed that OsDREB1D was able to form a complex with the dehydration responsive element/C-repeat motif. It can also bind with a sequence of LTRE (low temperature responsive element). To analyze the function of OsDREB1D, the gene was transformed and over-expressed in Arabidopsis thaliana cv. Columbia. Results indicated that the over-expression of OsDREB1D conferred cold and high-salt tolerance in transgenic plants, and that transgenic plants were also insensitive to ABA (abscisic acid). From these data, we deduced that this OsDREB1D gene functions similarly as other DREB transcription factors. The expression of OsDREB1D in rice may be controlled by a special mechanism for the redundancy of function.

• Journal of the Korea Society of Computer and Information
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• v.15 no.8
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• pp.23-30
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• 2010

Flash memory is non-volatile and can retain data even after system is powered off. Besides, it has many other features such as fast access speed, low power consumption, attractive shock resistance, small size, and light-weight. As its price decreases and capacity increases, the flash memory is expected to be widely used in consumer electronics, embedded systems, and mobile devices. Flash storage systems generally adopt a software layer, called FTL. In this research, we proposed a new FTL mechanism for overcoming the major drawback of conventional block mapping algorithm. In addition to the block mapping table, a index block mapping table with a small size is used to indicate sector location. The proposed indexed block mapping algorithm by adding a small size. By the simulation result, the proposed FTL provides an enhanced speed than a conventional hybrid mapping algorithm by around 45% in average, and the requirement of mapping memory is also reduced by around 12%.

• Journal of Microbiology
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• v.45 no.3
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• pp.227-233
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• 2007

In budding yeast, septin plays as a scaffold to recruits protein components and regulates crucial cellular events including bud site selection, bud morphogenesis, Cdc28 activation pathway, and cytokinesis. Phosphorylation of Bni5 isolated as a suppressor for septin defect is essential to Swe1-dependent regulation of bud morphogenesis and mitotic entry. The mechanism by which Bni5 regulates normal septin function is not completely understood. Here, we provide evidence that Bni5 phosphorylation is important for interaction with septin component Cdc11 and for timely delocalization from septin filament at late mitosis. Phosphorylation-deficient bni5-4A was synthetically lethal with $hof1{\Delta}$. bni5-4A cells had defective structure of septin ring and connected cell morphology, indicative of defects in cytokinesis. Two-hybrid analysis revealed that bni5-4A has a defect in direct interaction with Cdc11 and Cdc12. GFP-tagged bni5-4A was normally localized at mother-bud neck of budded cells before middle of mitosis. In contrast, at large-budded telophase cells, bni5-4A-GFP was defective in localization and disappeared from the neck approximately 2 min earlier than that of wild type, as evidenced by time-lapse analysis. Therefore, earlier delocalization of bni5-4A from septin filament is consistent with phosphorylation-dependent interaction with the septin component. These results suggest that timely de localization of Bni5 by phosphorylation is important for septin function and regulation of cytokinesis.