• Asian-Australasian Journal of Animal Sciences
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• 제12권1호
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• pp.9-14
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• 1999

Factors affecting gene gun-mediated expression of the human erythropoietin gene were investigated in primary cultured oviduct cells from laying hens. The human erythropoietin gene was transfected by a gene gun method at $1.25{\mu}g$ per dish, and cultured in a synthetic serum-free medium for 72 hrs. The concentration of human erythropoietin mRNA was determined by RNA : RNA solution hybridization. In experiment 1, the effect of changing the shooting pressure of DNA-coated microparticles with nitrogen gas was tested at 20 and $60kgf/cm^2$. The results showed that the erythropoietin mRNA concentration was significantly higher at 60 than $20kgf/cm^2$. In experiment 2, the effects of supplementing the medium with fetal calf serum at 10%, and raising the shooting pressure from 60 to $80kgf/cm^2$ on the cell number and erythropoietin gene expression were examined. Although supplementation with fetal calf serum significantly increased the cell numbes compared with no supplemented controls (p < 0.05), erythropoietin mRNA concentration per $10^3$ cells was not affected. Raising the shooting pressure from 60 to $80kgf/cm^2$ did not affect either of the parameters, In experiment 3, the effect of supplementing ascorbate 2-phosphate at 0.5 mM was tested. The results indicated that the ascorbate supplementation significantly increased the cell number (p < 0.05), and tended to increase erythropoietin mRNA concentration (p < 0.1). Thus, for human erythropoietin gene expression by using the gene gun method, shooting pressure with nitrogen gas should be sufficient at $60kgf/cm^2$ and supplementation with ascorbate phosphate would be useful to enhance not only the cell proliferation but also erythropoietin gene expression.

• 전기학회논문지
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• 제60권6호
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• pp.1190-1195
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• 2011

This paper presents a waist pressure evaluation method in human sensibility using a electroencephalogram(EEG) signal. For this objective, a size-controllable waist-belt is used. First of all, EEG signals for relaxed state are acquired. Then, the waist-belt of the subject is tightened about 90% of normal state. After a few minutes, the belt of the subject is released. Some necessary preprocessing is performed on the acquired signals, Linear Prediction (LP) coefficients are utilized as the feature parameters extracting the characteristics of EEG signal, and a multi-layer neural network is used for indicating the state of body pressure. The results of the method showed 77.2% of coincidence with body pressure states. This may be compromising results for ssubject-independent sensibility evaluation using EEG signal.

• 한국정밀공학회지
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• 제24권5호
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• pp.118-126
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• 2007

A finite element simulation model was developed for the performance optimization of a closed type air-cell mattress used for the ulcer prevention. An H-model with material properties of human flesh and kinematic joints were used for the calculation of the body contact pressure. The material property of rubber air-cell was evaluated by tensile test of standard specimen. We evaluated the body contact pressure distribution after laying human model on the inflated air-cell mattress. It was found that the body contact pressure was dependent on cell height. but hardly affected by the thickness of the rubber in a cell.

• Archives of Plastic Surgery
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• 제41권4호
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• pp.387-393
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• 2014

Background Reconstruction of ischial pressure sore defects is challenging due to extensive bursas and high recurrence rates. In this study, we simultaneously applied a muscle flap that covered the exposed ischium and large bursa with sufficient muscular volume and a profunda femoris artery perforator fasciocutaneous flap for the management of ischial pressure sores. Methods We retrospectively analyzed data from 14 patients (16 ischial sores) whose ischial defects had been reconstructed using both a profunda femoris artery perforator flap and a muscle flap between January 2006 and February 2014. We compared patient characteristics, operative procedure, and clinical course. Results All flaps survived the entire follow-up period. Seven patients (50%) had a history of surgery at the site of the ischial pressure sore. The mean age of the patients included was 52.8 years (range, 18-85 years). The mean follow-up period was 27.9 months (range, 3-57 months). In two patients, a biceps femoris muscle flap was used, while a gracilis muscle flap was used in the remaining patients. In four cases (25%), wound dehiscence occurred, but healed without further complication after resuturing. Additionally, congestion occurred in one case (6%), but resolved with conservative treatment. Among 16 cases, there was only one (6%) recurrence at 34 months. Conclusions The combination of a profunda femoris artery perforator fasciocutaneous flap and muscle flap for the treatment of ischial pressure sores provided pliability, adequate bulkiness and few long-term complications. Therefore, this may be used as an alternative treatment method for ischial pressure sores.

• 센서학회지
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• 제24권4호
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• pp.239-246
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• 2015

Blood pressure is possible to diagnose a disease associated with blood pressure and judgment the current health of patients. Automatic blood pressure monitor capable of measuring a blood pressure easily in hospital and at home have become spread. In this study, we developed the blood pressure simulator (BPS) that can test the arm-type automatic blood pressure monitor that is commonly used in hospital. BPS is to produce a pressure similar to the pressure wave generated in the human blood using a servo disk motor. Then, using the silicon tube, it implements the situations such as human blood vessels, and to output the generated pressure waveform. Simply the BPS's phantom put on the cuff and it is able to simulate blood pressure. So anyone can quickly test the blood pressure monitor within one minute and it is possible to shorten the test time required for the automatic blood pressure monitor. In Performance test, the trends and the standard deviation of the values measured in the BPS is similar to the value of the measured pressure from people with normal blood pressure. Thus, the development BPS showed a possibility of taking into account the actual blood pressure measurement environment simulator.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2009년도 춘계학술대회 논문집
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• pp.985-986
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• 2009

• 한국생활과학회지
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• 제11권1호
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• pp.79-93
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• 2002

The purpose of this study was to determine the effect of dynamic characteristics of knitted fabrics on the clothing pressure of foundation. Five knitted fabrics of foundation were selected by different fiber types and blended ratios for the study. The dynamic characteristics of foundation wears made with five different fabrics were measured by KES-FB system method. The results of this study were as follows: 1. The tensile linearity and resilience of f1 were lower than those of other samples. While the tensile resistances of f1, f2 and f4 samples were high, the coefficients of friction of them were low. The bending rigidity and geometric roughness of f5 were higher than the others. 2. The clothing pressure of f3 all-in-one sample was the highest, and followed by f5, f4, f2 and f1, respectively. This result showed that the compression resilience affected on the clothing pressure greatly. 3. In terms of the clothing pressure by posture, the clothing pressure at $90^{\circ}$ bending posture was the highest and followed by that at standing and one-leg-up, respectively. The back part of abdomen was pressured most at $90^{\circ}$ bending posture.

• 한국생활과학회지
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• 제5권1호
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• pp.15-31
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• 1996

The purpose of this study is to investigate the relationship to the life-style, perceived time pressure and time management strategies of married women. The research issues are as follow : 1. How are Life-styles influence to the perceived time pressure according to employment status of marraged women? 2. How are life-styles influence to the time manegement strategies according to employment status of marraged women? 3. How much are the effect of life-style, perceived time pressure and the rest of variable on the time management strategies of marraged women? For the purpose of this study, 585 questionnaire were distributed to marraged women living in pusan. The data were analyzed by statistical methods such as Pearson's correlation, percentage, factor analysis, and regression analysis. The significant results are as follow : 1. According to the employment status, there were several differences among those life-styles which are influencing to the perceived time pressure. 2. According to the employment status, there were differences among those life-styles which are influencing to the time management strategies. 3. Independent variables about the time management strategies of married women showed life-style(B=.46), age(B=-.18), income(B=.15), perceived time pressure(B=.12). education level(B=.07). The results of this study can be attributed to develop the efficient strategy of reducing perceived time pressure of marraged women.

• Archives of Plastic Surgery
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• 제38권3호
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• pp.235-240
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• 2011

Purpose: Management of pressure sores has been improved, along with development of musculocutaneous flaps and perforator flaps. Nowadays, the treatment of pressure sore with perforator flaps has shown several advantages, including minimal donor site morbidity, relatively versatile flap design not only in primary cases but also in recurred cases and minimized anatomical rearrangement of regional muscle position. In this study, we report our clinical experience of gluteal perforator flap used in the treatment of a greater trochanteric pressure sore. Methods: A clinical study was performed on 7 patients who underwent total 10 operations. 1 superior gluteal artery perforator flap and 9 inferior gluteal artery perforator flaps were used to reconstruct the defect, followed by the mean observation duration of 22 months. Results: There were no total flap loss. We treated 2 cases of partial flap loss with debridement and primary repair. 2 recurred cases were successfully treated using the same method. Donor sites were all primarily repaired. Conclusion: The gluteal perforator flap could be considered as a safe and favorable alternative in the treatment of soft tissue defects in the greater trochanteric area. The advantages of the flap include low donor site morbidity and the possibility of versatile flap design not only in primary cases but also in recurred cases.

• 한국생활과학회지
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• 제15권3호
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• pp.481-490
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• 2006

Adjusting pressure level in the construction of athletes' tight-fitting garments by reducing the elastic knit pattern is a challenging subject, which influences the performance of the wearer directly. Therefore, in this study, relationship between the reduction rates of the basic pattern obtained from 3D human scan data and resultant clothing pressure was explored to improve the fit and pressure exerted by clothing. 3D scan data were obtained using Cyberware and they were transformed into a flat pattern using software based on Runge-Kutta method. Reduction rate was examined by subjective wear test as well as objective pressure measurement. As a result, difference in the length between the original 3D body scan data and the 2D tight-fitting pattern was 0.02$\sim$0.50cm (0.05$\sim$1.06%), which was within the range of tolerable limits in making clothes. Among the five garments, the 3T-pattern was superior in terms of subjective sensation and fit. The pressure of the 3T pattern was 2$\sim$4 gf/cm2 at five locations on the body, which is almost the same or a bit higher than that of Z-pattern. In the case of tight-fitting overall garment, the reduction rate of the pattern in the wale direction is more critical to the subjective sensation than the course direction. It is recommended that the reduction grading rules of course direction should be larger than that of Ziegert for a better fit of tight-fitting garments. In the case of wale direction, however, reduction grading rule should be kept the same as suggested earlier by Ziegert (1988).