• Asian Pacific Journal of Cancer Prevention
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• 제13권9호
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• pp.4267-4272
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• 2012

Purpose: The aim of this study was to identify the opinions of Turkish adolesecent girls and their parents about HPV vaccination and the consistency. Methods: This descriptive study covered 301 girls and their parents. Questionnares were developed by the researchers based on findings within the literature and applied for data collection. Results: The mean age was 19.4 for girls, 44.2 for mothers and 47.9 for fathers. It was found that 43.5 percent of girls and 31.9 percent of mothers wish to be vaccinated against HPV. Also, 45.5 percent of mothers and 44.9 percent of fathers wish for their daughters to be vaccinated against HPV. A moderate consistency was found between mothers and fathers; a low consistency was found between fathers and girls, and between mothers and girls. Conclusions: The study indicates that an appropriate background has been partially provided about the acceptability of the vaccine between parents and their daughters in Turkey. However, the vast majority of adolescent girls and parents are indecisive or reluctant about HPV vaccination. This study also showed that the decisions of adolescents about vaccination may be affected by the opinions of the parents.

• 가정∙방문간호학회지
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• 제22권2호
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• pp.291-299
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• 2015

Purpose: The purpose of this study was to examine the influences of sex-relatedl knowledge, sex-related attitude and knowledge of cervical cancer on knowledge of human papilloma virus (HPV) among female high school students. Methods: A cross-sectional descriptive study was conducted with a convenience sample of 545 second-grade female high school students of three different schools. Results: Knowledge of HPV was positively correlated with sex-related knowledge (r=.36, p<.001), sex-related attitude (r=.14 p=.001) and knowledge of cervical cancer (r=.62, p<.001). Significant predictors affecting knowledge of HPV among female high school students were knowledge of cervical cancer (${\beta}$=.57) and sex-related knowledge (${\beta}$=.11), explaining 39.6% of the variance in knowledge of cervical cancer among female high school students (F=178.34, p<.001). Conclusion: Based on the outcomes of this study, in order to improve knowledge of HPV among female high school students, school based sexual education linked to HPV and cervical cancer must be included in the curriculum.

• 대한한방부인과학회지
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• 제25권4호
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• pp.154-160
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• 2012

Objectives: This report is aimed to investigate the efficacy of Korean medicine including intravenous Cultivated Wild Ginseng(Panax ginseng C.A. Meyer) Pharmacopuncture(CWGP) with intractable cervical dysplasia patient. Methods: A 49-year-old woman who was diagnosed as cervical dysplasia on Feb 2012 was treated with Korean medicine for 3 months. Korean medicine includes intravenous CWGP, acupuncture, moxibustion and herbal medicine. The effect of therapies was evaluated with human papillomavirus deoxyribonucleic acid(HPV DNA) testing. And lower limb cold sense and genital pruritus were recorded with Visual Analogue Scale(VAS). Results: Lower limb cold sense and genital pruritus were decreased from VAS 8-9(2012.04.11.) to VAS 1-2(2012.07.10.). HPV DNA test result was changed from positive to negative. From these results, this report suggests that the Korean medicine, especially CWGP may be a useful method to treat intractable cervical dysplasia. Conclusions: This report shows that Korean medical treatments are effective on intractable cervical dysplasia.

• Asian Pacific Journal of Cancer Prevention
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• 제13권1호
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• pp.339-342
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• 2012

Background: About one third of the human population suffer cancer during their lifetime and more than 20% of total morbidity is related to neoplasia. Cervical cancer is generally the most common cancer in developing countries and the second most common in women globally. The role of human papilloma viruses viruses in its induction is clear. However, the involvement of hepres simplex virus type 2 (HSV-2) is controversial. Therefore a survey was conducted of the prevalence of HSV-2 in patients with cervical cancer and also healthy people with sensitive and quantitative Taq Man real-time PCR assay. Materials and methods: Seventy six formaldehyde fixed paraffin embedded tissue specimens from patients with histologically proven history of cervical cancer as well as 150 control blocks were sectioned for deparaffinization and DNA extraction. Results: There was no HSV-2 DNA in our patient specimens but four control samples were positive, all with a history of hysterectomy. Conclusion: Considering the absence of any positive viral HSV-2 DNA in our patients and also the presence of four positive specimens among our controls, we did not find any relationship between the presence of HSV-2 DNA and cervical cancer.

• Asian Pacific Journal of Cancer Prevention
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• 제17권11호
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• pp.4959-4963
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• 2016

Objective:Cervical cancer is one of the major causes of mortality of Uyghur women in Xinjiang, China. Although increased expression of orexin receptor (OxR), known to be strongly expressed in human placenta, has a proven relation to some cancers, there have been few studies of cervical cancer. Thus, we explored this question by evaluating the expression of orexin receptor as a biomarker for screening early stage of cervical cancer in Uyghur women with highest occurrence rate of cervical cancer in China. Study Design: We used polymerase chain reaction (PCR) and immunohistochemical staining to determine the expression of both Ox1R and Ox2R in cervical cancer and cervicitis biopsies collected from Uyghur women infected with human papilloma virus (HPV)16. The expression rate was compared between cervical cancers of low, intermediate and high differentiation and cervicitis. Results:Although there was no significant difference in the expression rate of Ox1R between groups, Ox2R was significantly overexpressed in cervical cancer patients when compared to the cervicitis group. Ox1R was negative in normal human placenta while Ox2R was positive. Conclusions: While expression of Ox1R had no correlation with invasion or metastatic potential, Ox2R demonstrated elevation in cervical cancer with heterogeneity in groups with different metastatic potential, in the human placenta as well, implying that it might serve as an indicator of invasive capacity along with other indices.

• Journal of Periodontal and Implant Science
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• 제32권3호
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• pp.603-614
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• 2002

Human gingival fibroblasts have proven to useful as a species specific cell culture system in various system on periodontal disease and regeneration. However, their use is limited, since they are hard to obtain and lifespan is short due to replicative senescence. To overcome these disadvantages, we transfected primary human gingival fibroblasts by the E6 and E7 genes of the Human papilloma virus(HPV) 16. The full length of HPV 16 E6 and E7 was cloned from the pBR322 into BamHl and Sal I of a pBabe vector including hygromycin B resistance. Before pBabeE6/E7 plasmid transfection, peak 8 GFP including G418 resistance was transfected into primary GF to check the transfection efficency. PBabe E6/E7 plasmid was transfected using Lipofectamine plus following manufacter's instruction into primary normal human gingival fibroblasts in 60mm dishes with FBS free DMEM. After 2 days of transfection, the cells were treated with hygromycin for 2 weeks until the transfected control cells died. The resulting hygromycin resistant colonies were pooled, and clonned, and sucessful　transfection was established for immortalized gingival fibroblast cell lines. Immoralized GF cells showed stellate shape, that is similar to that of orange grains, and more rapid growth and higher proliferation than that of primary gingival fibroblasts. This cell lines overcame crisis and could be cultured over 30 subcultured, could be use for three dimentional culture, epithelial-mesenchymal interaction study.

• Journal of Microbiology and Biotechnology
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• 제22권5호
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• pp.721-728
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• 2012

The human papillomavirus (HPV) is the main cause of cervical cancer in developing countries. Rapid diagnosis and initiation of treatment of the HPV infection are critical. Various methods have been employed to reduce the immunogenicity of antibodies targeting HPV serotypes. Nanobodies are the smallest fragments of naturally occurring single-domain antibodies with their antigen-binding site compromised into a single domain. Nanobodies have remarkable properties such as high stability, solubility, and high homology to the human VH3 domain. In this study, a phagemid library was employed to enrich for nanobodies against the L1 protein of the human papilloma virus. Binding reactivity of the selected clones was evaluated using phage enzyme-linked immunosorbent assay (phage-ELISA). Finally, two nanobodies (sm5 and sm8) with the best reactivity against the Gardasil vaccine and the purified HPV-16 L1 protein were expressed and purified using a $Ni^+$-NTA column. The accuracy of expression and purification of the nanobodies was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting assays. In vitro studies demonstrated that neutralization was achieved by the selected nanobodies. The ease of generation and unique features of these molecules make nanobodies promising molecules for the new generation of HPV diagnosis and therapy.

• Asian Pacific Journal of Cancer Prevention
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• 제14권6호
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• pp.3641-3645
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• 2013

Background: Assessment of the nursing staff knowledge, attitude and practices about cervical cancer screening in a tertiary care teaching institute of rural India. Materials and Methods: A cross sectional, descriptive, interview-based survey was conducted with a pretested questionnaire among 262 staff nurses of a tertiary care teaching and research institute. Results: In this study 77% respondents knew that Pap smear is used for detection of cervical cancer, but less than half knew that Pap smear can detect even precancerous lesions of cervix. Only 23.4% knew human papilloma virus infection as a risk factor. Only 26.7% of the respondents were judged as having adequate knowledge based on scores allotted for questions evaluating knowledge about cervical cancer and screening. Only 17 (7%) of the staff nurses had themselves been screened by Pap smear, while 85% had never taken a Pap smear of a patient. Adequate knowledge of cervical cancer and screening, higher parity and age >30 years were significantly associated with self screening for cervical cancer. Most nurese held a view that Pap test is a doctor procedure, and nearly 90% of nurses had never referred a patient for Pap testing. Conclusions: The majority of nursing staff in rural India may have inadequate knowledge about cervical cancer screening, and their attitude and practices towards cervical cancer screening could not be termed positive.

• 한방안이비인후피부과학회지
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• 제33권2호
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• pp.174-185
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• 2020

Objectives : Atopic dermatitis is a chronic inflammatory disease characterized by severe pruritus, and may be accompanied by Molluscum Contagiosum, Verruca Vulgaris. This paper aims to examine the clinical implications of the treatment of 3 cases of atopic dermatitis with Molluscum Contagiosum, Verruca Vulgaris. Methods : 3 patients were treated by herbal medicine, herbal acupuncture, acupuncture, moxibustion and external preparations. Photographs of lesions, VAS were used to evaluate the changes in symptoms. Results : Atopic dermatitis is accompanied by Molluscum Contagiosum or Verruca Vulgaris because the underlying cell layer is easily exposed to Molluscum Contagiosum and Human papilloma virus because of scatch by pruritus. And all three cases have been well recovered by korean medical treatment. Conclusions : This study shows that Korean medical treatment is effective to treat atopic dermatitis with Molluscum Contagiosum, Verruca Vulgaris.

• 대한후두음성언어의학회지
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• 제27권1호
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• pp.5-10
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• 2016

Variable systemic diseases affect larynx and vocal fold and result in voice change. Asthma and chronic obstructive pulmonary disease make increase of intra-abdomimal pressure followed by reflux of gastric acid, which stimulate vagal-bronchopulomary reflex aggravating cough and respiratory disturbance. Fungal laryngitis in the general population is extremely rare, but can occur in immunocompromised AIDS patients. Although, initially, empirical antifungal therapy for candidiasis is often given without biopsy, diagnostic direct laryngoscopy and biopsy is imperative if a substantial clinical response is not rapidly achieved. In the highly active anti-retroviral therapy era, HIV-positive patients are living longer and are at higher risk for developing non-AIDS-defining malignancies. The incidence of head and neck cancer (HNC) which is related with human papilloma virus infection has increased. The survival is significantly lower among the AIDS-HNC patients with CD4 counts ${\leq}200cells/{\mu}L$. Rheumatoid arthritis (RA) cause voice disturbance by developing cricoarytenoid joints fixation or nodule on vocal fold. Post-menopausal voice disorder (PMVD) is caused by decreased secretion of estrogen-progesterone resulting in decrease of fundamental frequency (F0). Hormonal replacement therapy is helpful to reduce F0 decrease. RA and PMVD result in slight voice change, but it could crucial in professional voice user.