• Biomolecules & Therapeutics
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• v.20 no.2
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• pp.177-182
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• 2012

Tetrazolium violet is a tetrazolium salt and has been proposed as an antitumor agent. In this study, we reported for the first time that tetrazolium violet not only inhibited human lung cancer A549 cell proliferation but also induced apoptosis and blocked cell cycle progression in the G1 phase. The results showed that tetrazolium violet significantly decreased the viability of A549 cells at $5-15{\mu}M$. Tetrazolium violet -induced apoptosis in A549 cells was confirmed by H33258 staining assay. In A549, tetrazolium violet blocked the progression of the cell cycle at G1 phase by inducing p53 expression and further up-regulating p21/WAF1 expression. In addition, an enhancement in Fas/APO-1 and its two forms of ligands, membrane-bound Fas ligand (mFasL) and soluble Fas ligand (sFasL), as well as caspase, were responsible for the apoptotic effect induced by tetrazolium violet. The conclusion of this study is that tetrazolium violet induced p53 expression which caused cell cycle arrest and apoptosis. These findings suggest that tetrazolium violet has strong potential for development as an agent for treatment lung cancer.

• Molecules and Cells
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• v.45 no.12
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• pp.923-934
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• 2022

Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have great potential in applications such as regenerative medicine, cardiac disease modeling, and in vitro drug evaluation. However, hPSC-CMs are immature, which limits their applications. During development, the maturation of CMs is accompanied by a decline in their proliferative capacity. This phenomenon suggests that regulating the cell cycle may facilitate the maturation of hPSC-CMs. Aurora kinases are essential kinases that regulate the cell cycle, the role of which is not well studied in hPSC-CM maturation. Here, we demonstrate that CYC116, an inhibitor of Aurora kinases, significantly promotes the maturation of CMs derived from both human embryonic stem cells (H1 and H9) and iPSCs (induced PSCs) (UC013), resulting in increased expression of genes related to cardiomyocyte function, better organization of the sarcomere, increased sarcomere length, increased number of mitochondria, and enhanced physiological function of the cells. In addition, a number of other Aurora kinase inhibitors have also been found to promote the maturation of hPSC-CMs. Our data suggest that blocking aurora kinase activity and regulating cell cycle progression may promote the maturation of hPSC-CMs.

• Journal of Environmental Health Sciences
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• v.34 no.1
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• pp.62-69
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• 2008

The life cycle assessment method for environmental impact assessment was used, in this study, to assess the production process of wheat flour which is the most important material in the food industry. Environmental impact assessments were compared between that of the Ministry of Environment, Republic of Korea (method I) with that of the Ministry of Commerce, Industry and Energy (method II). Life cycle inventories (LCI) was performed using internal and external databases and the production statistics database of company S. The procedure of life cycle impact assessment (LCIA) was followed in terms of classification, characterization, normalization and weighting to identify the key issues. The impact categories of method I were divided into 8 categories with consideration of : abiotic resources depletion, global warming, ozone depletion, photochemical oxidant creation, acidification and eutrophication. The impact categories of method II were divided into 10 categories with consideration of: abiotic resources depletion, global warming, ozone depletion, photochemical oxidant creation, acidification, eutrophication, human toxicity, freshwater aquatic ecotoxicity, marine aquatic ecotoxicity and terrestrial ecotoxicity.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 1999.04a
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• pp.305-312
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• 1999

This study is intended to propose a systematic and practical life cycle cost(LCC) model for the development of the reliability-based seismic safety and cost-effective performance criteria for design and upgrading of long-span PC bridges. The LCC models consist of five cost functions such as initial cost, repair/replacement cost, human losses, road user cost, and indirect losses of regional economy. The proposed model Is successfully expressed in temrs of Park-Ang damage indices and life cycle damage probability obtained from SMART-DRAIN-2DX which is an existing algorithm for nonlinear time history analysis. The proposed LCC model is successfully applied to a viaduct constructed by PSM, in Seoul. Based on the observations, the proposed systematic procedure for the formulation of LCC model may be useful for the development of the reliability-based seismic safety and cost-effective performance criteria for design and upgrading of long-span PC bridges.

• International Journal of Human Ecology
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• v.11 no.1
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• pp.65-74
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• 2010

Family professionals and family program staff need to consider the importance of program evaluation in Korea since an increasing number of Healthy Family Support Centers are providing diverse intervention and education programs. The purpose of this research paper is to (a) introduce a program evaluation model that includes the program life cycle; (b) help family professionals and family program staff understand the link between program implementation and evaluation processes; and (c) facilitate discussions in terms of program evaluation of Healthy Family Support Centers and evaluation roles of different levels of Healthy Family Support Centers including the headquarters, regional, and local centers. Understanding the program life cycle and relevant evaluation processes will help family professionals and family program staff be more strategic in answering critical questions about a program's effectiveness. The benefits of program evaluation and its implications are discussed.

• Biomedical Science Letters
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• v.15 no.2
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• pp.147-152
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• 2009

Lycopus lucid us Turcz is well known as traditional Chinese medicine, and it has been shown to exhibit antiinflammatory, -allergic and -oxidative effect. However, its anti-cancer properties have not been examined yet. In this study, we investigated the effect of the methanol extract of Lycopus lucid us Turcz on anti-cancer effect in MCF-7 human breast cancer cells. Treatment of Lycopus lucidus Turcz extract induced apoptosis and inhibition of cell proliferation in dose- and time-dependent manner. Apoptosis in the MCF-7 cells was characterized with the changes in nuclear morphology; decrease of Bcl-2 and caspase-7 expression; and increase of cleaved poly ADP-ribose polymerase(PARP). Furthermore, treatment of Lycopus lucidus Turcz extract caused the down-regulation of cell cycle-related protein including, cdk4, cyclin D1 and E2F-1. These results suggest that Lycopus lucidus Turcz might have the therapeutic value against human breast cancer cells.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.18
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• pp.8113-8117
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• 2016

Glehnia littoralis (GL) is widely used as an oriental medicine for cough, fever, stroke and other disease conditions. However, the anti-cancer properties of GL on MCF-7 human breast cancer cells have not been investigated. In order to elucidate anti-cancer properties and underlying cell death mechanisms, MCF-7cells ($5{\times}10^4/well$) were treated with Glehnia littoralis root extract at 0-400 ug/ml. A hot water extract of GL root inhibited the proliferation of MCF-7 cells in a dose-dependent manner. Analysis of the cell cycle after treatment of MCF-7 cells with increasing concentrations of GL root extract for 24 hours showed significant cell cycle arrest in the G1 phase. RT-PCR and Western blot analysis both revealed that GL root extract significantly increased the expression of p21 and p27 with an accompanying decrease in both CDK4 and cyclin D1. Our reuslts indicated that GL root extract arrested the proliferation of MCF-7 cells in G1 phase through inhibition of CDK4 and cyclin D1 via increased induction of p21 and p27. In summary, the current study showed that GL could serve as a potential source of chemotherapeutic or chemopreventative agents against human breast cancer.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.18 no.34
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• pp.173-183
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• 1995

Life cycle cost has been one of the key criteria in design or purchasing of systems, particularly in the flying weapon system. Unexpected cost increase or system breakdown during the system life can be reduced by controlling maintenance cost A system should be designed for maintainability in early stage of product life cycle. The design should be insensitive to its environmental, organizational, and human factors in the stage of customer's utilization. This paper presents LCC as a controllable variable and also suggests a new control model for LCC analysis. The estimation of maintenance cost based upon maintenance scenario, design of maintainability followed by minimizing maintainability loss function in the beginning stage of design, and increase of useful life of systems are among the factors to control LCC.

• Computers and Concrete
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• v.25 no.2
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• pp.167-179
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• 2020

Optimal design of structures against earthquake loads is often limited to reduce initial construction costs, while the cost induced to structures during their useful life may be several times greater than the initial costs. Therefore, it is necessary to consider the indirect costs due to earthquakes in the design process. In this research, an integrated methodology for calculating life cycle cost (LCC) of moment-resisting concrete frames is presented. Increasing seismic safety of structures and reducing human casualties can play an important role in determining the optimal design. Costs incurred for structures are added to the costs of construction, including the costs of reconstruction, financial losses due to the time spent on reconstruction, interruption in building functionality, the value of people's life or disability, and content loss are a major part of the future costs. In this research, fifty years of useful life of structures from the beginning of the construction is considered as the life cycle. These costs should be considered as factors of calculating indirect costs of a structure. The results of this work represent the life cycle cost of a 4 story, 7 story, and 10 story moment-resisting concrete frame by details. This methodology is developed based on the economic conditions of Iran in 2016 and for the case of Tehran city.

• Journal of Families and Better Life
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• v.18 no.2
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• pp.109-123
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• 2000

The purpose of this study is to investigate decision making styles task management of urban homemakers. This study focuses on the following aspects: 1) to find out which variables of background variables (ie. age of couple, education of couple, duration of marriage, income, family life cycle, number of children) have effect on decision making styles & task management of urban homemakers. 2) to find out the relationships between decision making styles and task management of urban homemakers. 3) to find out independent influcene of background variables on task management. major findings are as follows; First, it is general tendency that decision making styles were human oriented in urban homemakers. Importance & Urgency of the task, age of homemakers, education of homemakers, family life cycle were significant variables to have influcence on task oriented decision making styles. Importance & urgency of task were variables to have influences on human oriented decision making styl s. Second, the tendency of dealing with task at once is the highest in the task management, on the other hand, the tendency of dealing with task in the next session is the lowest. Income is a variable to influence on dealing with task in delegation to others, education of homemaker is a variable to influence on dealing with task in next session and importance & urgency of the task and the number of children were variables to influence on dealing with task immediately. Third, human oriented decision marking style was a variable to influence on dealing with task in delegation with task in delegation to others, in the next session, and in suspending/ in neglecting. Task oriented decision making style was a variable to influence on dealing with task immediately. Fourth, The urgency of task & income were variables to have direct influence on dealing with task in delegation with task in next session, and the urgency of the task, age & education of homemaker and family life cycle were varia les to direct influence on dealing with the task immediately. The urgency & importance of the task and age of homemaker were variables to have indirect on dealing with the task urgency of task & the number of children were variables to have indirect influence on dealing with the task in suspending/in neglecting through the medium of the human oriented decision making styles.