• 대한본초학회지
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• 제20권3호
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• pp.51-58
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• 2005

Objectives : The purpose of this research was physiological activities and investigate cytotoxicity of Sunbanghwalmyung-um extract. Methods : Physiological activity and a cytotoxicity were examined through the hot water and ethanol extracts from Sunbanghwalmyung-um and its ingredient. Results : The electron donating ability(EDA) was 66.9%, 71.3% in 1000 ppm water extract and ethanol extract. Above 90% from 1000 ppm ethanol extract showed a higher activities and it is Rhei Radix et Rhizoma, Lonicerae Flos, Gleditsiae Spina, Glycyrrhizae Radix, Myrrh. SOD-like activity was weak as 12.24%, 16.62% in 700 ppm. In water and ethanol extracts cytotoxicity were against G361, B16F10, MDA, A549, high cytotoxity over 70%. Rhei Radix et Rhizoma, Gleditsiae Spina, Trichosanthis Radix, Paeoniae Radix Rubra showed high cytotoxicity in water and ethanol extracts. Conclusions : We observed physiological activities and investigated cytotoxicity of Sunbanghwalmyung-um and its ingredients. The results also demonstrated in food or cosmetic industry.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.312-312
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• 2017

The study was conducted to select optimal materials for promoting germination rate, high sprouting vigor by priming treatment using PEG 6000 (water potential -0.5 to 2.0 Mpa) and Azolla extracts (0.1, 1.0, and 10.0%) extracted by ethanol, distilled, and/or hot water in colored rice cultivars. Each rice seed (three black rice cultivars and two red rice cultivars) was soaked 24 and 48 hr including untreated control. In black rice, Joseongheugchal rice cultivar, azolla ethanol extract (0.1%) induced highest germination rate, germination speed was taken to 5 days in distilled water and to 3days in Azolla extracts extracted hot water. Otherwise, degree of bacterial inhibition (number of colony, $10^3cfu$) in dry seed, water soaking for 24hr, soaking with fungicide for 24hr and 48hr, soaking with fungicide and aeration for 24hr and 48 hr was 22, 500, 95, and 0.46, respectively. In order to minimize fungal inhibition, a method can be chosen to combination of soaking fungicide and aeration for 48 hr. In seed priming treatments using growth pouch, seed soaking with fungicide did not affect change of germination percentage and germination speed, it delayed only 2 or 4day in the Joseongheugchal rice cultivar. It differs from rice cultivars and priming materials, Azolla extract(0.1 to 1%) promotes seed germination percentage in the Ilpum, Hongjinju, and Joseonghuegchal, in addition, germination in Jeogjinju cultivar was only promoted by PEG solution(10 to 20%), otherwise, it showed much lower or inhibited on the germination in Heugjinju and Sinmyungheugchal rice cultivars. In a paddy field trial, seedling establishment rate by applying PEG6000 and azolla extract did not show significantly statistical difference. When it compared with untreated control, seedling establishment rate was increased over 50% in priming treatments. Interestingly, seedling establishment rate under azolla extract (0.1%) extracted with ethanol was promoted over 2.5 times compared to the control in a black rice, Joseongheugchal and red rice, Jeogjinju.

• 생명과학회지
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• 제21권8호
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• pp.1190-1198
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• 2011

한약재의 추출방법에 따른 인체신경모세포 SK-N-SH에 대한 보호 효과를 연구하였다. 당귀, 건지황, 작약 및 천궁을 시료로 사용하였고, 열수추출(환류냉각, 5 시간), 증숙추출(100$^{\circ}C$ 및 120$^{\circ}C$, 90분 후 열수추출) 방법과 에탄올추출(환류냉각, 5 시간)방법을 비교하였다. 추출물을 농도별로 SK-N-SH 세포에 2 시간 처리한 후 $H_2O_2$로 250${\mu}M$로 2 시간 산화스트레스를 유발한 다음 세포독성 및 apoptosis와 caspase-3의 발현 정도를 측정하였다. 모든 약재의 열수추출물이 다른 추출물보다 세포 증식을 촉진하였고, apoptosis를 억제하였다. 한약재 열수추출물 1${\mu}g/{\mu}l}$ 농도까지는 세포증식을 촉진하였지만, 그 이상의 농도에서는 오히려 감소시켰다. 열수추출물은 다른 추출물보다 총페놀성 화합물이 많이 함유되어 있었고, 항산화능이 높았다. 또한, 이와 같은 효과는 당귀의 열수추출물이 다른 약재 열수추출물보다 우수하였다. 본 연구결과는 약재의 열수추출법이 인체신경모세포인 SK-N-SH의 증식과 세포사멸 억제를 위해 가장 우수한 방법이었고, 당귀 열수추출물이 가장 우수한 효과를 지니고 있어 기억력 보호나 상실억제제로 활용할 수 있을 것이다.

• 한국자원식물학회지
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• 제11권2호
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• pp.173-178
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• 1998

The feeding effects of Herba hottuynia extracts on the lipids profiles and the content of TAB-reactive substances were evaluated in rats fed a high cholesterol diet. Hot water or ethanol extracts of the dried leave were evaporated and lyophilized . The experimental animals wer edevided to four groups and fed the following diets for 4 weeks : BAsal (cholesterol-free diet), CHOL(cholesterol-enriched diet),CW (cholesterol plus water extract) and CE(cholesterol plus ethanol extract). Dietary cholesterol increased significantly the activities of serum GOT and GPT, but the extracts feeding (0.5% of diet) did not influence the activities induced by dietary cholesterol. Although dietary cholesterol increased significantly the concentrationof serum andliver cholesterol, it tended to decrease the concentation of serum triglycerides. CHolesterol feeding had a lowering effect on the lipid peroxidation value of serum, but not inliver.Furthermore, the extracts feeding, especially water extract, decreased markedly the liver peroxidation value. The results suggest that Houttyunia cordata extracts have an in vivo antioxidant effect, judged from the TBA value in the liver rats fed a high cholesterol diet.

• 생명과학회지
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• 제28권9호
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• pp.1068-1075
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• 2018

흑생강은 생강과 초본과 뿌리식물로 태국 및 라오스에서는 크라차이담으로 불리며, 뿌리는 향신료와 차로 이용되어 왔다. 말린 뿌리는 위장장애, 통풍, 이질, 알러지 치료 및 강장용으로 전통적으로 이용되어 왔으며, 최근에는 항비만, 항산화, 항염증, 혈전 용해 활성 등의 다양한 유용 생리활성이 보고되어 있다. 본 연구에서는 흑생강의 혈액순환 개선 활성을 확인하기 위해, 흑생강 지하부의 ethanol 추출물 및 열수 추출물을 조제하고 각각 이의 순차적 유기용매 분획물인 hexane 분획물, ethylacetate (EA) 분획물, butanol 분획물 및 물 잔류물을 조제하여 각각의 성분을 분석하였고, 혈전 생성과 관련된 항응고 활성, 혈소판 응집저해 활성 및 적혈구 용혈활성을 평가하였다. 그 결과, ethanol 추출물의 EA 분획물 5 mg/ml 농도에서 TT, PT, aPTT를 각각 1.22, 1.49 및 15배 이상 연장시켜 강력한 항응고 활성을 나타냄을 확인하였으며, ethanol 및 열수 추출물의 EA 분획물 모두에서 동량의 아스피린보다 강력한 혈소판 응집저해 활성을 확인하였다. 또한 상기 EA 분획물들은 0.5 mg/ml 농도까지 인간 적혈구에 대한 용혈활성을 나타내지 않았다. 본 연구 결과는 흑생강의 혈전 생성 억제 활성에 대한 최초의 보고이며, 흑생강의 EA 분획물이 인간 적혈구 용혈활성 없이 강력한 항응고 및 혈소판 응집저해 활성을 나타내어 신규의 항혈전제로 이용 가능함을 제시하고 있다.

• International Journal of Industrial Entomology and Biomaterials
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• 제14권2호
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• pp.121-126
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• 2007

Obesity is a major risk factor for cardiovascular disease. In our case study using animal models for disease states such as obesity or hypertension, we found that, Isaria sinclairii remarkably reduced body weight and ameliorated metabolic abnormalities in Zucker and SHR rats. Genetically obese (fa/fa) Zucker rats were one animal model chosen for this study. Four groups of rats received a standard diet and were treated orally with the following test samples daily for 8 weeks: saline (negative control), ethanol extracts of I. sinclairii, hot water extract of I. sinclairii, or $Xenical^{(R)}$ (30 mg/kg, positive control). Mild reductions (6.3%) in body weight gain were observed in the groups treated with the hot water extract of I. sinclairii compared to the control after 8 weeks. Interestingly, organ weight was greatly reduced by this Dongchunghacho (I. sinclairii), in parallel with the mild reductions in body weight gain and reductions in abdominal fat (adipose tissue). Also observed was a 4.1 % decrease in the ratio of heart weight/body weight compared to the control group. As a hypertensive animal model, SHR (spontaneously hypertensive rat) and WKY (Wistar Kyoto) rats were also administered these extracts for one month. Treatment with the hot water extract of I. sinclairii caused greater reductions in body weight gain for the SHR group (10.9%) compared to the WKY group's (5.2%). Based on these results, I. sinclairii extracts contain selective action for anti-obesity activity, naturally occurring candidate for regulation of body weight increase, as demonstrated in the present study.

• 한국식품저장유통학회지
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• 제11권3호
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• pp.319-324
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• 2004

감귤과피의 열수, 에탄을 및 설탕 추출방법에 따른 항균활성을 비교 조사하였다 그 결과 감귤껍질의 열수 및 에탄올 추출물에서는 항균활성이 나타나지 않았으나, 설탕추출물에서 높은 항균활성을 나타내었다. 10$\%$(w/v) 설탕용액에서 9일간 추출하였을 때 8종의 균주 모두에서 항균활성이 가장 높았다 또한 최소저해농도는 S. aureus에서 0.5$\%$(v/v), B. subtilis, M. luteus 및 E. coli는 1.5%(viv), S. mutans는2.0$\%$(v/v) 각각 나타났다. 감귤 껄질 추출물은 40$\~$100$^{\circ}C$, 1시간 처리범위에서 항균성은 매우 안정하였으나, pH의 변화에서는 불안정하게 나타났다. 이상의 결과로 감귤껍질 추출물은 열안정성이 높은 항균성 소재로 개발이 기대되었다.

• 한국지역사회생활과학회지
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• 제27권3호
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• pp.343-350
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• 2016

The antioxidant activity was examined on ultra-fine powdered (UFP) Ulmus davidiana (U. davidiana) var. japonica. The average particle size of UFP or commercial powdered (CP) was $1-3{\mu}m$ or $100{\mu}m$. The extraction was performed using either ethanol (EtOH) or hot-water. Contents of phenolic compound of CP and UFP U. davidiana extracts in EtOH was 40.38 and 65.61 mg/g, respectively. In DPPH, EtOH extract of UFP U. davidiana showed a significantly greater activity than hot-water extract at 40 and $200{\mu}g/mL$. At $200{\mu}g/mL$, the activity was over 90% in all groups. The reducing power of UFP U. davidiana var. japonica in EtOH extraction was 74.3%, which was significantly greater than in other samples (p<0.05). In addition, reducing powder was significantly higher in UFP-EtOH than in other samples at all concentrations except for $0.32{\mu}g/mL$. The above results suggest that EtOH extraction of U. davidiana showed slightly higher DPPH radical scavenging activity, and ultra-fine powder of U. davidiana extracts may show higher antioxidative activities based on reducing power.

• Journal of the Korean Wood Science and Technology
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• 제45권5호
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• pp.495-510
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• 2017

본 연구는 삼나무 잎 추출물의 항산화 활성을 평가하여 잠재적인 천연 항산화제로의 적용 가능성 구명에 관한 연구이다. 삼나무 잎 추출물은 세 가지 추출방법에 의해 정유, 메탄올 추출물, 열수 추출물로 획득하여 항산화 활성을 평가 하였고, 대조구와 비교하여 DPPH radical 소거활성, FRAP 활성, zanthin oxidase 저해활성, 철이온 chelate 활성 평가를 실시하였다. 그 결과, 메탄올 추출물과 열수 추출물의 항산화 활성이 정유의 항산화 활성보다 우수했다. 정유가 함유하고 있는 테르펜 화합물은 항산화 활성이 비교적 낮은 성분으로 사료된다. 활성이 우수했던 메탄올추출물과 열수 추출물은 다양한 유기용매(n-hexane, chloroform, ethyl acetate, n-butanol, methanol, ethanol, acetone)와 물을 사용하여 분획을 실시하였고, 메탄올 추출물의 acetone 및 물 분획물, 그리고 열수 추출물의 에탄올 분획물이 천연 항산화 성분으로 알려진 ${\alpha}$-tocopherol과 유사한 활성을 나타내거나, 보다 우수한 항산화 활성을 나타냈다. 이들은 공통적으로 수산기 및 당류를 가지는 성분을 함유하고 높은 페놀성 화합물 함량을 나타냈기 때문에 삼나무 잎 추출물은 수산기 및 당류를 가지는 페놀성 화합물에 의해 항산화 활성을 나타내는 것이라 판단되었다.

• Journal of Korean Biological Nursing Science
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• 제23권1호
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• pp.1-10
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• 2021

Purpose: To verify the anti-microbiotic activity and to develop eco-friendly surface disinfectants using the Opuntia ficus-indica extract. Methods: The stem and fruits of the Opuntia cultivated in Jeju Island were extracted with hot water, 70% EtOH and 100% MeOH, respectively. To examine the antimicrobial activity of the extracts, gram positive bacteria (Bacillus subtilis, Staphylococcus aureus), gram negative bacteria (Escherichia coli, Salmonella typhimurium), yeast (Candida albicans), mold (Aspergillus flavus) and antibiotic resistant bacteria (E. coli, S. aureus) were measured to form a clear zone. Results: The antibacterial activity of the fruit extract was higher than that of the stem extract, and the antibacterial activity of the 100% MeOH (FM) extract and the 70% EtOH (FE) extract of the palm cactus fruit was suitable for the surface disinfectant overall, and clear-zone formed even at relatively low concentrations (250 mg/mL). The FM and FE have antibacterial activity against various bacteria, therefore they can be used as disinfectants. Conclusion: The antimicrobial activity of Opuntia ficus-indica extract from Jeju Island by stem and fruit was determined at relatively low concentrations (250 mg/mL). The Opuntia ficus-indica extract will be useful for surface disinfectants dissolving the patients' bath, oral gargling, and powdered powder in water or ethanol in appropriate concentrations.