Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
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Antioxidant Activities of Cryptomeria japonica Leaves Extracts by Extraction Methods

추출 방법에 따른 삼나무 잎 추출물의 항산화 활성 평가

  • Kim, Seon-Hong (Research Institute for Agriculture & Life Sciences, College of Agriculture & Life Sciences, Seoul National University) ;
  • Lee, Su-Yeon (Division of Wood Chemistry & Microbiology, Dept. of Forest Resources Utilization, Korea Forest Research Institute) ;
  • Cho, Seong-Min (Department of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University) ;
  • Hong, Chang-Young (Division of Wood Chemistry & Microbiology, Dept. of Forest Resources Utilization, Korea Forest Research Institute) ;
  • Park, Se-Yeong (Department of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University) ;
  • Park, Mi-Jin (Division of Wood Chemistry & Microbiology, Dept. of Forest Resources Utilization, Korea Forest Research Institute) ;
  • Choi, In-Gyu (Research Institute for Agriculture & Life Sciences, College of Agriculture & Life Sciences, Seoul National University)
  • 김선홍 (서울대학교 농업생명과학연구원) ;
  • 이수연 (국립산림과학원 임산공학부 화학미생물과) ;
  • 조성민 (서울대학교 농업생명과학대학 산림과학부) ;
  • 홍창영 (국립산림과학원 임산공학부 화학미생물과) ;
  • 박세영 (서울대학교 농업생명과학대학 산림과학부) ;
  • 박미진 (국립산림과학원 임산공학부 화학미생물과) ;
  • 최인규 (서울대학교 농업생명과학연구원)
  • Received : 2017.05.26
  • Accepted : 2017.06.15
  • Published : 2017.09.25
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Abstract

This study was to investigate the antioxidant activities of Cryptomeria japonica leaves extracts such as essential oil, methanol extract and hot water extract and to evaluate its potential as a natural antioxidant. Antioxidant activities of extracts were evaluated by DPPH radical scavenging activity, FRAP activity, zanthin oxidase inhibitory activity, and iron ion chelate activity, comparing with the positive controls. According to the results, methanol and hot water extracts showed higher antioxidant activities than essential oil. This fact suggested that terpenoids have lower antioxidant effect than phenolic compounds which were found in methanol and hot water extracts. Especially, acetone and water soluble fractions of methanol extract and ethanol fraction of hot water extract contained a lot of phenolic compounds and hydroxyl group, indicating that many hydroxyl groups and glycosidic bonds showed higher antioxidant effect than the other fractions. These results can suggest that the phenolic compounds which contained hydroxyl group or glycosidic bonds have a certain role for effective antioxidant activities.

본 연구는 삼나무 잎 추출물의 항산화 활성을 평가하여 잠재적인 천연 항산화제로의 적용 가능성 구명에 관한 연구이다. 삼나무 잎 추출물은 세 가지 추출방법에 의해 정유, 메탄올 추출물, 열수 추출물로 획득하여 항산화 활성을 평가 하였고, 대조구와 비교하여 DPPH radical 소거활성, FRAP 활성, zanthin oxidase 저해활성, 철이온 chelate 활성 평가를 실시하였다. 그 결과, 메탄올 추출물과 열수 추출물의 항산화 활성이 정유의 항산화 활성보다 우수했다. 정유가 함유하고 있는 테르펜 화합물은 항산화 활성이 비교적 낮은 성분으로 사료된다. 활성이 우수했던 메탄올추출물과 열수 추출물은 다양한 유기용매(n-hexane, chloroform, ethyl acetate, n-butanol, methanol, ethanol, acetone)와 물을 사용하여 분획을 실시하였고, 메탄올 추출물의 acetone 및 물 분획물, 그리고 열수 추출물의 에탄올 분획물이 천연 항산화 성분으로 알려진 ${\alpha}$-tocopherol과 유사한 활성을 나타내거나, 보다 우수한 항산화 활성을 나타냈다. 이들은 공통적으로 수산기 및 당류를 가지는 성분을 함유하고 높은 페놀성 화합물 함량을 나타냈기 때문에 삼나무 잎 추출물은 수산기 및 당류를 가지는 페놀성 화합물에 의해 항산화 활성을 나타내는 것이라 판단되었다.

Keywords

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