• Title/Summary/Keyword: host-based

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Efficient Screening Method for Resistance of Cucumber Cultivars to Fusarium oxysporum f. sp. cucumerinum (오이 덩굴쪼김병에 대한 효율적인 저항성 검정 방법)

  • Lee, Ji Hyun;Kim, Jin-Cheol;Jang, Kyoung Soo;Choi, Yong Ho;Choi, Gyung Ja
    • Research in Plant Disease
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    • v.20 no.4
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    • pp.245-252
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    • 2014
  • The study was performed to establish an efficient screening method for resistant cucumber to Fusarium oxysporum f. sp. cucumerinum. The isolate KR5 was identified as F. oxysporum f. sp. cucumerinum based on molecular analyses of ITS and TEF genes and host-specificity test on cucurbits including melon, oriental melon, cucumber, and watermelon. Then four cucumber and two rootstock cultivars showing different resistance degrees to the Fusarium wilt pathogen KR5 were selected. And development of Fusarium wilt of the six cultivars according to several conditions, including incubation temperature after inoculation, inoculum concentration, root wounding, and growth stages of seedlings, was investigated. Disease severity of Fusarium wilt on the resistant cultivars was changed with incubation temperatures after inoculation. The resistant cultivars showed the higher resistance when inoculated plants were kept at 25 or $30^{\circ}C$ than at $20^{\circ}C$. Among four different growth stages of the seedlings, seven-day-old seedling represented the most difference of resistance and susceptibility to Fusarium wilt. From above results, we suggest that an efficient screening method for resistant cucumber to F. oxysporum f. sp. cucumerinum is to dip the non-cut roots of seven-day-old seedlings in spore suspension of $1.0{\times}10^6-1.0{\times}10^7$ conidia/ml and to transplant the seedling into a non-infected soil, and then to incubate the inoculated plants in a growth room at $25^{\circ}C$ for 3 weeks to develop Fusarium wilt.

Selection of Representative Magnaporthe oryzae Isolates and Rice Resistant Gene Types for Screening of Blast-resistant Rice Cultivars (우리나라 벼 도열병균의 대표 균주 및 벼의 저항성 유전자형 선발)

  • Goh, Jaeduk;Kim, Byung-Ryun;Lee, Se-Won;Roh, Jae-Hwan;Shin, Dong-Bum;Jeung, Ji-Ung;Cho, Young-Chan;Han, Seong-Sook
    • Research in Plant Disease
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    • v.19 no.4
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    • pp.243-253
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    • 2013
  • Rice blast is one of the most serious disease threatening stable production of rice. Breeding of resistant cultivars has been used as the most effective and useful method to controll rice blast caused by Magnaporthe oryzae. To collect rice blast isolates in fields and test their pathogenicity on new cultivars are important for establishment of new resistant cultivars breeding program of rice. Pathotypes of Korean rice blast isolates have been categorized to Korean differential race system developed in 1985. However, it is little known about genetic background of Korean differential cultivars, so that it is hard to understand for relationship between each pathogen and each host plant at genetic level. In this study, we suggested necessity of a new differential system by analyzing pathogenic responses between 24 monogenic rice lines and 200 Korean rice blast isolates. In addition, we determined the nine representative resistant genes based on the resistance responses of the monogenic lines to rice blast isolates, indexed resistant responses of the monogenic lines to ten representative rice blast isolates and selected 30 Korean representative rice blast isolates proper to Korean system. We think the newly developed differential race system can be broadly used to select resistant cultivars to rice blast in Korea.

Analyses of the Double-Layered Repository Concepts for Spent Nuclear Fuels (사용후핵연료 심지층 처분장 복층개념 분석)

  • Lee, Jongyoul;Kim, Hyeona;Lee, Minsoo;Choi, Heui-Joo;Kim, Kyungsu
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.15 no.2
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    • pp.151-159
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    • 2017
  • A deep geological disposal at a depth of 500 m in stable host rock is considered to be the safest method with current technologies for disposal of spent fuels classified as high-level radioactive waste. The most important requirement is that the temperature of the bentonite buffer, which is a component of the engineered barrier, should not exceed $100^{\circ}C$. In Korea, the amount of spent fuel generated by nuclear power generation, which accounts for about 30% of the total electricity, is continuously increasing and accumulating. Accordingly, the area required to dispose of it is also increasing. In this study, various duplex disposal concepts were derived for the purpose of improving the disposal efficiency by reducing the disposal area. Based on these concepts, thermal analyses were carried out to confirm whether the critical disposal system requirements were met, and the thermal stability of the disposal system was evaluated by analyzing the results. The results showed that upward 75 m or downward 75 m apart from the reference disposal system location of 500 m depth would qualify for the double layered disposal concept. The results of this study can be applied to the establishment of spent fuel management policy and the design of practical commercial disposal system. Detailed analyses with data of a real disposal site are necessary.

Engineering Geological Implications of Fault Zone in Deep Drill Cores: Microtextural Characterization of Pseudotachylite and Seismic Activity (시추코어 단층대에서의 지질공학적 의미: 슈도타킬라이트의 미세조직의 특징과 지진활동)

  • Choo, Chang-Oh;Jeong, Gyo-Cheol
    • The Journal of Engineering Geology
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    • v.27 no.4
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    • pp.489-500
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    • 2017
  • It is not rare that pseudotachylite, dark colored rock with glassy texture, is recognizable in deep core samples drilled up to 900 m from the surface. Pseudotachylite with widths varying few to 20 cm is sharply contacted or interlayered with the host rocks composed of Jurassic granite and Precambrian amphibolite gneiss, showing moderately ductile deformation or slight folding. Pseudotachylite occurring at varying depths in the deep drill core are slightly different in texture and thickness. There is evidence of fault gouge at shallower depths, although brittle deformation is pervasive in most drill cores and pseudotachylite is identified at random depth intervals. Under scanning electron microscope (SEM), it is evident that the surface of pseudotachylite is characterized by a smooth, glassy matrix even at micrometer scale and there is little residual fragments in the glass matrix except microcrystals of quartz with embayed shape. Such textural evidence strongly supports the idea that the pseudotachylite was generated through the friction melting related to strong seismic events. Based on X-ray diffraction (XRD) quantitative analysis, it consists of primary minerals such as quartz, feldspars, biotite, amphibole and secondary minerals including clay minerals, calcite and glassy materials. Such mineralogical features of fractured materials including pseudotachylite indicate that the fractured zone might form at low temperatures possibly below $300^{\circ}C$, which implies that the seismic activity related to the formation of pseudotachylite took place at shallow depths, possibly at most 10 km. Identification and characterization of pseudotachylite provide insight into a better understanding of the paleoseismic activity of deep grounds and fundamental information on the stability of candidate disposal sites for high-level radioactive waste.

Extraction of the ship movement information by a radar target extractor (Radar Target Extractor에 의한 선박운동정보의 추출에 관한 연구)

  • Lee, Dae-Jae;Kim, Kwang-Sik;Byun, Duck-Soo
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.38 no.3
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    • pp.249-255
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    • 2002
  • This paper describes on the extraction of ship's real-time movement information using a combination full-function ARPA radar and ECS system that displays radar images and an electronic chart together on a single PC screen. The radar target extractor(RTX) board, developed by Marine Electronics Corporation of Korea, receives radar video, trigger, antenna bearing pulse and heading pulse signals from a radar unit and processes these signals to extract target information. The target data extracted from each pulse repetition interval in DSPs of RTX that installed in 16 bit ISA slot of a IBM PC compatible computer is formatted into a series of radar target messages. These messages are then transmitted to the host PC and displayed on a single screen. The position data of target in range and azimuth direction are stored and used for determining the center of the distributed target by arithmetic averaging after the detection of the target end. In this system, the electronic chart or radar screens can be displayed separately or simulaneously and in radar mode all information of radar targets can be recorded and replayed In spite of a PC based radar system, all essential information required for safe and efficient navigation of ship can be provided.

Enterococcus faecium LKE12 Cell-Free Extract Accelerates Host Plant Growth via Gibberellin and Indole-3-Acetic Acid Secretion

  • Lee, Ko-Eun;Radhakrishnan, Ramalingam;Kang, Sang-Mo;You, Young-Hyun;Joo, Gil-Jae;Lee, In-Jung;Ko, Jae-Hwan;Kim, Jin-Ho
    • Journal of Microbiology and Biotechnology
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    • v.25 no.9
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    • pp.1467-1475
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    • 2015
  • The use of microbial extracts containing plant hormones is a promising technique to improve crop growth. Little is known about the effect of bacterial cell-free extracts on plant growth promotion. This study, based on phytohormonal analyses, aimed at exploring the potential mechanisms by which Enterococcus faecium LKE12 enhances plant growth in oriental melon. A bacterial strain, LKE12, was isolated from soil, and further identified as E. faecium by 16S rDNA sequencing and phylogenetic analysis. The plant growth-promoting ability of an LKE12 bacterial culture was tested in a gibberellin (GA)-deficient rice dwarf mutant (waito-C) and a normal GA biosynthesis rice cultivar (Hwayongbyeo). E. faecium LKE12 significantly improved the length and biomass of rice shoots in both normal and dwarf cultivars through the secretion of an array of gibberellins (GA1, GA3, GA7, GA8, GA9, GA12, GA19, GA20, GA24, and GA53), as well as indole-3-acetic acid (IAA). To the best of our knowledge, this is the first study indicating that E. faecium can produce GAs. Increases in shoot and root lengths, plant fresh weight, and chlorophyll content promoted by E. faecium LKE12 and its cell-free extract inoculated in oriental melon plants revealed a favorable interaction of E. faecium LKE12 with plants. Higher plant growth rates and nutrient contents of magnesium, calcium, sodium, iron, manganese, silicon, zinc, and nitrogen were found in cell-free extract-treated plants than in control plants. The results of the current study suggest that E. faecium LKE12 promotes plant growth by producing GAs and IAA; interestingly, the exogenous application of its cell-free culture extract can be a potential strategy to accelerate plant growth.

Potential repository domain for A-KRS at KURT facility site (KURT 부지 조건에서 A-KRS 입지 영역 도출)

  • Kim, Kyung-Su;Park, Kyung-Woo;Kim, Geon-Young;Choi, Heui-Joo
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.10 no.3
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    • pp.151-159
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    • 2012
  • The potential repository domains for A-KRS (Advanced Korean Reference Disposal System for High Level Wastes) in geological characteristics of KURT (KAERI Underground Research Tunnel) facility site were proposed to develop a repository system design and to perform the safety assessment. The host rock of KURT facility site is one of major Mesozoic plutonic rocks in Korean peninsula, two-mica granite, which was influenced by hydrothermal alteration. The topographical features control the flow lines of surface and groundwater toward south-easterly and all waters discharge to Geum River. Fracture zones distributed in study site are classified into order 2 magnitude and their dominant orientations are N-S and E-W strike. From the geological features and fracture zones, the potential repository domains for A-KRS were determined spatially based on the following conditions: (1) fracture zone must not cross the repository; and (2) the repository must stay away from the fracture zones greater than 50 m. The western region of the fracture zones in the N-S direction with a depth below 200 m from the surface was sufficient for A-KRS repository. Because most of the fracture zones in N-S direction were inclined toward the east, we expected to find a homogeneous rock mass in the western region rather than in the eastern region. The lower left domain of potential domains has more suitable geological and hydrogeological conditions for A-KRS repository.

A Maximum Mechanism of Data Transfer Rate using Parallel Transmission Technology on High Performance Network (고성능 네트워크에서 병렬 전송 기술을 이용한 전송률 극대화 메커니즘)

  • Kim, Young-Shin;Huh, Eui-Nam
    • Journal of KIISE:Computer Systems and Theory
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    • v.34 no.9
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    • pp.425-434
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    • 2007
  • Even though Internet backbone speeds have increased in the last few years due to projects like Internet 2 and NGI, many high performance distributed applications are able to achieve only a small fraction of the available bandwidth. The cause of such problem is due to a character of TCP/IP. The primary goal of this protocol is reliable data transmission. Therefore high speed data transmission didn't be considered when TCP/IP is designed. Hence several researchers have been studied in order to solve the problem of TCP/IP. One of these research results, parallel transfer technique, solves this problem to use parallel TCP connections on application level. Additionally, this technique is compatibility. Recently, these researchers have been studied a mechanism to decide the number of parallel TCP connections. However, some researchers reported the number of parallel TCP connection base on only empirical results. Although hardware performance of host affects transmission rate, the hardware performance didn't be considered in their works. Hence, we collect all data related to transmission rate, such as hardware state information (cpu utilization, interrupt, context switch). Then, we analyzed collected data. And, we suggest a new mechanism determining number of parallel TCP connections for maximization of performance based on our analysis.

Selection of Biocontrol Agent of Tomato Gray Mold Disease from Flower and Pollinator Hive (토마토 꽃과 수정용 벌집으로부터 잿빛곰팡이병 방제 길항균주 선발)

  • Kim, Da-Ran;Lee, Jun-Taek;Kim, Hye sun;Jeon, Chang Wook;Kwak, Youn-Sig
    • The Korean Journal of Pesticide Science
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    • v.21 no.1
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    • pp.90-96
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    • 2017
  • Gray mold disease, cause by Botrytis cinerea, occurs severe damage on varieties of fruit and vegetable production, and have no a critical control method. In case of chemicals control, it is a trigger emergence of drug resistance strains due to using them continuously. In addition, the pathogen is difficult to control naturally because it is possible to survive regardless of host status. In this study, microorganisms were isolated from tomato flower and hive samples and in order to select suitable microbial control agents for tomato gray mold disease. During six-months study, we isolated 1,004 isolates from flower and 925 isolates from pollinator hive samples. Among them, 6 strains were selected based on result of antifungal activity test. The selected strains showed not only strong antifungal activity against gray mold pathogen, but also cellulase and protease enzyme activities. The selected strains were identified as Paenibacillus polymyxa. In plant assay, P. polymyxa prevented the gray mold disease occurrence near 75%.

Reliable Extension Scheme using Multiple Paths in Wireless Ad-hoc Networks (무선 애드-혹 네트워크의 다중 경로를 이용한 신뢰적인 확장 기법)

  • Kim, Moon-Jeong;Eom, Young-Ik
    • Journal of KIISE:Information Networking
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    • v.34 no.3
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    • pp.218-225
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    • 2007
  • As the research on home network technologies, sensor network technologies, and ubiquitous network technologies makes rapid progresses, wireless ad-hoc network have attracted a lot of attention. A wireless mobile ad-hoc network is a temporary network formed by a collection of wireless mobile nodes without the aid of any existing network infrastructure or centralized administration, and it is suitable for ubiquitous computing environments. In this paper, we suggest an extension scheme of a wireless mobile ad-hoc network based on limited multiple paths source routing protocol. This scheme reduces the overhead of route re-establishment and re-registration by maintaining link/node non-disjoint multiple paths between mobile hosts in a wireless mobile ad-hoc network or a mobile host in a wireless mobile ad-hoc network and a base station supporting fixed network services. By maintaining multiple paths, our scheme provides short end-to-end delay and is reliable extension scheme of a wireless mobile ad-hoc network to a fixed network. In this paper, our simulations show that our scheme outperforms existing schemes with regards to throughput and end-to-end delay. Also we show that our scheme outperforms multi-paths approach using disjoint routes with regards to routing overhead.