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Selection of Representative Magnaporthe oryzae Isolates and Rice Resistant Gene Types for Screening of Blast-resistant Rice Cultivars

우리나라 벼 도열병균의 대표 균주 및 벼의 저항성 유전자형 선발

  • Goh, Jaeduk (Crop Environment Research Division, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Byung-Ryun (Agricultural Environment Research Division, Chungcheongnam-do Agriculture Research & Extension Services) ;
  • Lee, Se-Won (Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Roh, Jae-Hwan (Winter Cereal and Forage Crop Research Division, National Institute of Crop Science, Rural Development Administration) ;
  • Shin, Dong-Bum (Crop Environment Research Division, National Institute of Crop Science, Rural Development Administration) ;
  • Jeung, Ji-Ung (Rice Research Division, National Institute of Crop Science, Rural Development Administration) ;
  • Cho, Young-Chan (Rice Breeding and Cultivation Research Division, National Institute of Crop Science, Rural Development Administration) ;
  • Han, Seong-Sook (Crop Environment Research Division, National Institute of Crop Science, Rural Development Administration)
  • 고재덕 (국립식량과학원 작물환경과) ;
  • 김병련 (충남농업기술원 농업환경연구과) ;
  • 이세원 (국립농업과학원 농업미생물과) ;
  • 노재환 (국립식량과학원 맥류사료작물과) ;
  • 신동범 (국립식량과학원 작물환경과) ;
  • 정지웅 (국립식량과학원 답작과) ;
  • 조영찬 (국립식량과학원 벼육종재배과) ;
  • 한성숙 (국립식량과학원 작물환경과)
  • Received : 2013.09.03
  • Accepted : 2013.11.28
  • Published : 2013.12.31

Abstract

Rice blast is one of the most serious disease threatening stable production of rice. Breeding of resistant cultivars has been used as the most effective and useful method to controll rice blast caused by Magnaporthe oryzae. To collect rice blast isolates in fields and test their pathogenicity on new cultivars are important for establishment of new resistant cultivars breeding program of rice. Pathotypes of Korean rice blast isolates have been categorized to Korean differential race system developed in 1985. However, it is little known about genetic background of Korean differential cultivars, so that it is hard to understand for relationship between each pathogen and each host plant at genetic level. In this study, we suggested necessity of a new differential system by analyzing pathogenic responses between 24 monogenic rice lines and 200 Korean rice blast isolates. In addition, we determined the nine representative resistant genes based on the resistance responses of the monogenic lines to rice blast isolates, indexed resistant responses of the monogenic lines to ten representative rice blast isolates and selected 30 Korean representative rice blast isolates proper to Korean system. We think the newly developed differential race system can be broadly used to select resistant cultivars to rice blast in Korea.

벼 도열병은 안정적인 쌀 생산을 위협하는 대표적인 병으로, 도열병 방제에는 저항성 품종의 육종이 가장 효과적으로 이용되었다. 병원균의 꾸준한 수집과 이들을 이용한 저항성 검정 테스트는 신품종 육성 프로그램에서 중요한 과정이다. 1985년 이래 우리나라의 도열병균은 8개의 판별품종을 이용한 레이스 판별시스템을 구축하여 분류하였다. 그러나 기존의 판별품종은 유전자형이 연구된 바 없어 새로운 레이스 출현이나 병 저항성 붕괴 등에 대하여 과학적으로 분석하여 신속하게 대처하기가 어려웠다. 따라서 이 연구에서는 유전자형이 밝혀진 단인자 저항성 계통과 우리나라에서 수집된 200개 균주의 병원성 반응을 분석하여, 새로운 판별 품종 시스템의 필요성을 제시하였다. 또한, 24개의 단인자 저항성 계통의 병원성 반응을 통해 9개의 대표 저항성 유전자를 선발하였으며, 이에 따른 10개의 대표균주 그룹과 그 저항성 반응을 인덱스화 하고 30개의 한국형 대표균주를 선발하였다. 이 연구는 향후 도열병 저항성 검정 및 벼 신품종 육종에 기초자료로서 활용될 수 있을 것이다.

Keywords

References

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  2. Blast Resistant Genes Distribution and Resistance Reaction to Blast in Korean Landraces of Rice (Oryza sativa L.) vol.27, pp.6, 2014, https://doi.org/10.7732/kjpr.2014.27.6.687