• Journal of Electrochemical Science and Technology
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• 제14권1호
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• pp.51-58
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• 2023

The effective detection of human chorionic gonadotropin (HCG) is considerably important for the clinical diagnosis of both of early pregnancy and nonpregnancy-related diseases. In this work, a simple and sensitive electrochemical sandwich-type immunosensing platform was designed by synthesizing b-cyclodextrin (CD) functionalized graphene (CD/GN) hybrid as simultaneously sensing platform and signal transducer coupled with rhodamine b (RhB) as probe. In brief, GN offers large surface area and high conductivity, while CD exhibits superior host-guest recognition capability, thus the primary antibody (Ab1) of HCG can be bound into the cavities of CD/GN to form stable Ab1/CD/GN inclusion complex; meanwhile, the secondary antibody (Ab2) and RhB can also enter into the cavities, producing RhB/Ab2/CD/GN complex. Then, by using Ab1/CD/GN as sensing platform and RhB/Ab2/CD/GN as signal transducer (in which RhB was signal probe), a simple sandwich-type immunosensor was constructed. Under the optimum parameters, the designed immunosensor exhibited a considerable low analytical detection of 1.0 pg mL^-1 and a wide linearity of 0.002 to 10.0 ng mL^-1 for HCG, revealing the developed sandwich-type electrochemical immunosensing platform offered potential real applications for the determination of HCG.

• 한국미생물·생명공학회지
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• 제50권1호
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• pp.1-14
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• 2022

Bacteria communicate with each other through an intricate communication mechanism known as quorum sensing (QS). QS regulates different behavioral aspects in bacteria, such as biofilm formation, sporulation, virulence gene expression, antibiotic production, and bioluminescence. Several different chemical signals and signal detection systems play vital roles in promoting highly efficient intra- and interspecies communication. Gram-negative bacteria coordinate gene regulation through the production of acyl homoserine lactones (AHLs). Gram-positive bacteria do not code for AHL production, while some gram-negative bacteria have an incomplete AHL-QS system. Despite this fact, these microbes can detect AHLs owing to the presence of LuxR solo receptors. Various studies have reported the role of AHLs in interspecies signaling. Moreover, as bacteria live in a polymicrobial community, the production of extracellular compounds to compete for resources is imperative. Thus, AHL-mediated signaling and inhibition are considered to affect virulence in bacteria. In the current review, we focus on the synthesis and regulation mechanisms of AHLs and highlight their role in interspecies bacterial signaling. Exploring interspecies bacterial signaling will further help us understand host-pathogen interactions, thereby contributing to the development of therapeutic strategies intended to target chronic polymicrobial infections.

• 한국인터넷방송통신학회논문지
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• 제8권6호
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• pp.89-95
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• 2008

분산 컴퓨팅 환경에서 모바일 서로게이트 시스템은 보안상의 문제로 인하여 널리 사용되고 있지 못하고 있다. 현재 가장 널리 사용되고 있는 그리드 미들웨어인 글로버스에서도 모바일 단말기를 인증하는 방법이 제공되고 있지 않아 그리드의 강력한 컴퓨팅 능력을 모바일 클라이언트까지 확장하는 것을 어렵게 하는 중요한 요인이 되고 있다. 본 논문에서는 PDA와 같이 컴퓨팅 파워가 미약한 모바일 단말기기와 그리드 호스트 간의 인터페이스 역할을 하는 게이트웨이를 두고 모바일 클라이언트가 이동 중 그리드 서비스를 이용할 수 있도록 하는 안전한 모바일 그리드서비스 프레임워크를 설계 구현하였다. 아울러, 이를 구현하여 ECG 신호처리를 위한 헬스케어 시스템에 적용하였다. 이 시스템은 이동 환경에서 환자의 생체 신호를 그리드 컴퓨팅으로 분석하여 원격에 있는 의료진이 진단할 수 있는 모바일 헬스케어에 활용가능하다.

• Mycobiology
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• 제47권4호
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• pp.473-482
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• 2019

Rice blast disease, caused by the ascomycete fungus Magnaporthe oryzae, is one of the most important diseases in rice production. PAS (period circadian protein, aryl hydrocarbon receptor nuclear translocator protein, single-minded protein) domains are known to be involved in signal transduction pathways, but their functional roles have not been well studied in fungi. In this study, targeted gene deletion was carried out to investigate the functional roles of the PAS-containing gene MoPAS1 (MGG_02665) in M. oryzae. The deletion mutant ΔMopas1 exhibited easily wettable mycelia, reduced conidiation, and defects in appressorium formation and disease development compared to the wild type and complemented transformant. Exogenous cAMP restored appressorium formation in ΔMopas1, but the shape of the restored appressorium was irregular, indicating that MoPAS1 is involved in sensing the hydrophobic surface. To examine the expression and localization of MoPAS1 in M. oryzae during appressorium development and plant infection, we constructed a MoPAS1:GFP fusion construct. MoPAS1:GFP was observed in conidia and germ tubes at 0 and 2 h post-infection (hpi) on hydrophobic cover slips. By 8 hpi, most of the GFP signal was observed in the appressoria. During invasive growth in host cells, MoPAS1:GFP was found to be fully expressed in not only the appressoria but also invasive hyphae, suggesting that MoPAS may contribute to disease development in host cells. These results expand our knowledge of the roles of PAS-containing regulatory genes in the plant-pathogenic fungus M. oryzae.

• 대한전자공학회논문지SD
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• 제48권5호
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• pp.34-41
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• 2011

본 논문은 24 채널 정전 용량형 터치 검출 ASIC에 대한 것이다. 제안된 회로는 아날로그 회로부와 디지털 회로부로 구성되어 있다. 아날로그 회로부는 사용자의 접촉을 전기적인 신호로 변환시키며 디지털 회로부는 전기적인 신호의 변화를 디지털 데이터로 변환시키는 역할을 담당한다. 디지털 회로는 I2C가 내장되어 시스템 동작 계수들을 호스트 프로세서에서 변경해 줄 수 있도록 설계되었다. 따라서 온도 변화 등 외부환경 변화에도 안정적으로 동작할 수 있다. 본 ASIC은 0.18um CMOS 공정으로 구현되었으며 그 크기는 약 3 $mm^2$ 이고 소비전력은 5.3 mW이다. 설계에는 Cadence사와 Synopsys사의 상용 개발환경이 사용되었다.

• Molecules and Cells
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• 제28권4호
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• pp.365-368
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• 2009

Toll-like receptors (TLRs) play an important role in host defense by sensing invading microbial pathogens and initiating innate immune responses. The stimulation of TLRs by microbial components triggers the activation of myeloid differential factor 88 (MyD88)- and toll-interleukin-1 receptor domain-containing adapter inducing interferon-${\beta}$ (TRIF)-dependent downstream signaling pathways. Isoliquiritigenin (ILG), an active ingredient of Licorice, has been used for centuries to treat many chronic diseases. ILG inhibits the MyD88-dependent pathway by inhibiting the activity of inhibitor-${\kappa}B$ kinase. However, it is not known whether ILG inhibits the TRIF-dependent pathway. To evaluate the therapeutic potential of ILG, we examined its effect on signal transduction via the TRIF-dependent pathway of TLRs induced by several agonists. ILG inhibited nuclear factor-${\kappa}B$ and interferon regulatory factor 3 activation induced by lipopolysaccharide or polyinosinic-polycytidylic acid. ILG inhibited the lipopolysaccharide-induced phosphorylation of interferon regulatory factor 3 as well as interferon-inducible genes such as interferon inducible protein-10, and regulated activation of normal T-cell expressed and secreted (RANTES). These results suggest that ILG can modulate TRIF-dependent signaling pathways of TLRs, leading to decreased inflammatory gene expression.

• 한국음향학회지
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• 제16권2호
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• pp.10-15
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• 1997

수중 청음기는 진동하는 물체 위에 설치되어 다양한 외부 잡음 원이 유입되는 환경에 노출되어 있다. 외부 잡음 원으로는 수중 청음기가 설치된 구조물 자체의 진동, 프로펠러 잡음, 그리고 유동 유기 잡음들이 있고, 이들 외부 잡음원은 실제 강도가 상당히 높아서 센서의 정확한 작동에 장애가 되고 있다. 본 연구에서는 외부 잡음에 무관한 고 정밀도, 저 잡음 특성을 갖는 수중 청음기를 개발하기 위하여 유한 요소법(FEM)을 사용하여 잡음 전달 특성의 분석 및 air pocket과 음향 감쇠층의 다양한 조합으로 이루어진 개선된 구조의 수중 청음기를 설계하고, 내 잡음성 평가를 하였다. 그 결과 센서 측면 하단부에 잡음 원이 위치할 경우 가장 큰 잡음 신호로 작용하므로 구조를 변경한 결과 기존 수중 청음기에 비해 59% 이상 내 잡음성을 증진 시켰다.