Journal of Electrochemical Science and Technology

The Korean Electrochemical Society (한국전기화학회)
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Simple and Sensitive Electrochemical Sandwich-type Immunosensing of Human Chorionic Gonadotropin based on b-cyclodextrin Functionalized Graphene

  • Linfen Xu (Department of Gynaecology, Fujian Provincial Maternity and Children's Hospital, Affiliated Hospital of Fujian Medical University) ;
  • Ling liu (Department of reproduction, Fuzhou Maternity and Child Health care Hospital) ;
  • Xiaoyan Zhao (Department of Clinical Laboratory, Fujian Provincial Maternity and Children's Hospital, Affiliated Hospital of Fujian Medical University) ;
  • Jinyu Lin (Department of family planning, Fuzhou Maternity and Child Health care Hospital) ;
  • Shaohan Xu (Clinical lab, Fuzhou Maternity and Child Health care Hospital) ;
  • Jinlian He (Department of Obstetrics and Gynecology, Fuzhou Maternity and Child Health care Hospital) ;
  • Debin Jiang (Department of Cardiovascular Surgery, Union Hospital, Fujian Medical University) ;
  • Yong Xia (Department of Obstetrics and Gynecology, Fuzhou Maternity and Child Health care Hospital)
  • Received : 2022.07.16
  • Accepted : 2022.08.29
  • Published : 2023.02.28
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Abstract

The effective detection of human chorionic gonadotropin (HCG) is considerably important for the clinical diagnosis of both of early pregnancy and nonpregnancy-related diseases. In this work, a simple and sensitive electrochemical sandwich-type immunosensing platform was designed by synthesizing b-cyclodextrin (CD) functionalized graphene (CD/GN) hybrid as simultaneously sensing platform and signal transducer coupled with rhodamine b (RhB) as probe. In brief, GN offers large surface area and high conductivity, while CD exhibits superior host-guest recognition capability, thus the primary antibody (Ab1) of HCG can be bound into the cavities of CD/GN to form stable Ab1/CD/GN inclusion complex; meanwhile, the secondary antibody (Ab2) and RhB can also enter into the cavities, producing RhB/Ab2/CD/GN complex. Then, by using Ab1/CD/GN as sensing platform and RhB/Ab2/CD/GN as signal transducer (in which RhB was signal probe), a simple sandwich-type immunosensor was constructed. Under the optimum parameters, the designed immunosensor exhibited a considerable low analytical detection of 1.0 pg mL-1 and a wide linearity of 0.002 to 10.0 ng mL-1 for HCG, revealing the developed sandwich-type electrochemical immunosensing platform offered potential real applications for the determination of HCG.

Keywords

Acknowledgement

The work was financially supported by Fuzhou health technology project (2019-S-wp7).

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