• The Plant Pathology Journal
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• 제17권5호
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• pp.257-263
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• 2001

Species of the genus Orobanche are parasitic flowering plants, holoparasites, which cling to the roots of green plants. Their tiny seeds (200 x $250\mu\textrm{m}$) germinate in response to chemical stimuli produced by host and some non-host plants. Successful contact with their host leads to development of haustoria for obtaining water and food. The shoots above the ground expose flowers and disseminate seeds. Several samples of Orobanche ramosa, O. crenata, O. cernua, and O. egyptiaca were collected from different localities in Jordan. These samples showed one of the following disease symptoms: dry rot at the base of the stem; general deterioration and expanded lesion from base upward; soft tissue maceration of stem; and black rot of flower parts with incomplete maturation of the ovary and seeds. Isolation from diseased stems and seeds was made on three different mycological media. Several fungi were isolated, mainly, Fusarium spp., Alternaria alternata, Rhizoctonia sp., Dendrophora sp., Chaetomium sp., and an ascomycetus fungus with a perithecium. Pathogenicity tests showed that Fusarium spp. and Alternaria alternata attacked healthy living tissue of Orobanche spikes. These fungi caused lesions of black soft rot and complete deterioration within 5-7 days. They also attacked Orobanche seeds, arresting their germination and causing maceration of non-germinated and germinated seeds after 5-7 days of incubation. Meanwhile, Dendrophora sp. and Chaetomium sp. caused limited lesion at first, but were able to colonize the tissue as it aged and senesced. This study showed the presence of a potential endogenous pathogenic fungi in Jordan, which can be investigated as a biological control for Orobanche.

• Parasites, Hosts and Diseases
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• 제54권3호
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• pp.363-368
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• 2016

We describe here the mass death of predatory carp, Chanodichthys erythropterus, in Korea induced by plerocercoid larvae of Ligula intestinalis as a result of host manipulation. The carcasses of fish with ligulid larvae were first found in the river-edge areas of Chilgok-bo in Nakdong-gang (River), Korea at early February 2016. This ecological phenomena also occurred in the adjacent areas of 3 dams of Nakdong-gang, i.e., Gangjeong-bo, Dalseong-bo, and Hapcheon-Changnyeong-bo. Total 1,173 fish carcasses were collected from the 4 regions. To examine the cause of death, we captured 10 wondering carp in the river-edge areas of Hapcheon-Changnyeong-bo with a landing net. They were 24.0-28.5 cm in length and 147-257 g in weight, and had 2-11 plerocercoid larvae in the abdominal cavity. Their digestive organs were slender and empty, and reproductive organs were not observed at all. The plerocercoid larvae occupied almost all spaces of the abdominal cavity under the air bladders. The proportion of larvae per fish was 14.6-32.1% of body weight. The larvae were ivory-white, 21.5-63.0 cm long, and 6.0-13.8 g in weight. We suggest that the preference for the river-edge in infected fish during winter is a modified behavioral response by host manipulation of the tapeworm larvae. The life cycle of this tapeworm seems to be successfully continued as the infected fish can be easily eaten by avian definitive hosts.

• 한국가금학회지
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• 제39권1호
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• pp.17-25
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• 2012

Coccidiosis control programs such as vaccines or in-feed anticoccidials are commonly practiced in the poultry industry to improve growth performance and health of commercial broiler chickens. In this study, we assessed the effects of various coccidiosis control programs (e.g., in ovo vaccination, synthetic chemicals, and antibiotic ionophores) on immune status of broiler chickens vaccinated against infectious bronchitis virus and Newcastle disease virus (ND) and raised on an Eimeria-contaminated used litter. In general, the levels of ${\alpha}$-1-acid glycoprotein, an acute phase protein, were altered by the treatments when measured at 34 days of age. Splenocyte subpopulations and serum antibody titers against ND were altered by various coccidiosis control programs. In-ovo-vaccinated chickens exhibited highest mitogenic response when their spleen cells were stimulated with concanavalin A (Con A) at 7 days of age. It is clear from this study that the type of coccidiosis control program influenced various aspects of innate and adaptive immune parameters of broiler chickens. Further studies will be necessary to delineate the underlying relationship between the type of coccidiosis control program and host immune system and to understand the role of other external environmental factors such as gut microbiota on host-pathogen interaction in various disease control programs.

• Journal of Microbiology and Biotechnology
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• 제24권9호
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• pp.1269-1279
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• 2014

Xanthomonas oryzae pv. oryzae (Xoo) strains are plant pathogenic bacteria that can cause serious blight of rice, and their virulence towards plant host is complex, making it difficult to be elucidated. Caenorhabditis elegans has been used as a powerful model organism to simplify the host and pathogen system. However, whether the C. elegans is feasible for studying plant pathogens such as Xoo has not been explored. In the present work, we report that Xoo strains PXO99 and JXOIII reduce the lifespan of worms not through acute toxicity, but in an infectious manner; pathogens proliferate and persist in the intestinal lumen to cause marked anterior intestine distension. In addition, Xoo triggers (i) the p38 MAPK signal pathway to upregulate its downstream C17H12.8 expression, and (ii) the DAF-2/DAF-16 pathway to upregulate its downstream gene expressions of mtl-1 and sod-3 under the condition of daf-2 mutation. Our findings suggest that C. elegans can be used as a model to evaluate the virulence of Xoo phytopathogens to host.

• 한국멀티미디어학회논문지
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• 제10권4호
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• pp.421-431
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• 2007

이동 통신 기술의 급격한 발전으로, 이동 컴퓨팅 환경에서 데이터 서비스에 대한 수요가 점차 증가하고 있다. 이동 컴퓨팅 환경에서의 여러 단점을 극복하고자 캐쉬가 등장하게 되었다. 이동 호스트 내에 캐쉬가 존재하면, 통신 대역폭의 절약 및 질의에 대한 빠른 응답을 가져올 수 있지만, 이동 호스트가 서버 데이터와의 캐쉬 일관성(consistency)을 유지시켜야 한다는 문제가 생기게 된다. 본 논문에서는 기존의 기법들이 가지는 문제점들을 보완하기 위해 데이터의 주기성을 고려한 캐쉬 일관성 유지 기법을 제안한다. 제안한 기법은 데이터를 주기적, 비주기적 데이터로 분류하고 주기적 데이터에 만료시간을 삽입한다. 또한 무효화 메시지를 저장해서 단절 후에 선별적으로 캐쉬 데이터가 나누어질 수 있도록 해서, 캐쉬 내의 데이터가 접속 단절 후에 일방적으로 버려지는 것을 막을 수 있게 된다. 이렇게 함으로서 서버와 이동 호스트간의 통신에 사용하는 전체 대역폭의 낭비와 서버접속에 필요한 이동 호스트의 전력의 낭비를 줄일 수 있다. 마지막으로 기존의 기법과의 실험을 통해서 제안한 기법의 효율성을 알아보고자 한다.

• Journal of Microbiology and Biotechnology
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• 제26권4호
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• pp.684-692
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• 2016

Acute respiratory virus infectious diseases are a growing health problem, particularly among children and the elderly. Much effort has been made to develop probiotics that prevent influenza virus infections by enhancing innate immunity in the respiratory tract until vaccines are available. Lactobacillus plantarum GB-LP2, isolated from a traditional Korean fermented vegetable, has exhibited preventive effects on influenza virus infection in mice. To identify the molecular basis of this strain, we conducted a whole-genome assembly study. The single circular DNA chromosome of 3,284,304 bp was completely assembled and 3,250 protein-encoding genes were predicted. Evolutionarily accelerated genes related to the phenotypic trait of anti-infective activities for influenza virus were identified. These genes encode three integral membrane proteins, a teichoic acid export ATP-binding protein and a glucosamine - fructose-6-phosphate aminotransferase involved in host innate immunity, the nonspecific DNA-binding protein Dps, which protects bacteria from oxidative damage, and the response regulator of the three-component quorum-sensing regulatory system, which is related to the capacity of adhesion to the surface of the respiratory tract and competition with pathogens. This is the first study to identify the genetic backgrounds of the antiviral activity in L. plantarum strains. These findings provide insight into the anti-infective activities of L. plantarum and the development of preventive probiotics.

• IMMUNE NETWORK
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• 제4권2호
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• pp.73-80
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• 2004

Viruses are obligate intracellular parasites which cause infection by invading and replicating within cells. The immune system has mechanisms which can attack the virus in extracellular and intracellular phase of life cycle, and which involve both non-specific and specific effectors. The survival of viruses depends on the survival of their hosts, and therefore the immune system and viruses have evolved together. Immune responses to viral infection may be variable depending on the site of infection, the mechanism of cell-to-cell spread of virus, physiology of the host, host genetic variation, and environmental condition. Viral infection of cells directly stimulates the production of interferons and they induce antiviral state in the surrounding cells. Complement system is also involved in the elimination of viruses and establishes the first line of defence with other non-specific immunity. During the course of viral infection, antibody is most effective at an early stage, especially before the virus enters its target cells. The virus- specific cytotoxic T lymphocytes are the principal effector cells in clearing established viral infections. But many viruses have resistant mechanism to host immune responses in every step of viral infection to cells. Some viruses have immune evasion mechanism and establish latency or persistency indefinitely. Furthermore antibodies to some viruses can enhance the disease by the second infection. Immune responses to viral infection are very different from those to bacterial infection.

• Mycobiology
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• 제36권4호
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• pp.236-241
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• 2008

The colonization of an arbuscular mycorrhizal fungus Glomus intraradices BEG110 in the soil caused a decrease in disease severity in cucumber plants after fungal inoculation with Colletotrichum orbiculare. In order to illustrate the resistance mechanism mediated by G. intraradices BEG110, infection patterns caused by C. orbiculare in the leaves of cucumber plants and the host cellular responses were characterized. These properties were characterized using transmission electron microscopy on the leaves of cucumber plants grown in soil colonized with G. intraradices BEG110. In the untreated plants, inter- and intra-cellular fungal hyphae were observed throughout the leaf tissues during both the biotrophic and necrotrophic phases of infection. The cytoplasm of fungal hyphae appeared intact during the biotrophic phase, suggesting no defense response against the fungus. However, several typical resistance responses were observed in the plants when treated with G. intraradices BEG110 including the formation of sheaths around the intracellular hyphae or a thickening of host cell walls. These observations suggest that the resistance mediated by G. intraradices BEG110 most often occurs in the symplast of the host cells rather than in the apoplast. In addition, this resistance is similar to those mediated by biotic inducers such as plant growth promoting rhizobacteria.

• 정보처리학회논문지D
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• 제12D권1호
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• pp.21-30
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• 2005

이동 컴퓨팅 환경에서 이동 호스트는 대역폭의 사용을 줄이고 이동 트랜잭션의 응답 시간을 향상시키기 위하여 캐쉬를 사용한다. 그리고 서버는 이동 호스트의 캐쉬 일관성을 유지하기 위해서 주기적으로 갱신된 데이터를 방송한다. 그러나 무효화 보고서를 사용한 캐쉬 일관성 유지기법은 이동 트랜잭션의 완료 결정을 지연한다는 문제점을 가지고 있다. 본 논문에서는 캐슁된 데이터를 사용하여 이동 트랜잭션을 수행하는 경우 이동 트랜잭션의 직렬 가능한 수행을 보장하는 CCM-AD 방법을 제안한다. 제안하는 CCM-AD 방법은 서버에서 이동 호스트에게 방송한 데이터와 갱신된 데이터와의 교집합을 구하여 공통되는 데이터를 요청 데이터와 함께 방송함으로써 이동 트랜잭션의 직렬 가능한 수행을 보장한다. 그리고 수행 후 완료 결정을 위한 이동 트랜잭션의 지연도 줄일 수 있다. 또한 주기적으로 방송하는 무효화 보고서의 메시지 크기도 줄일 수 있다.

• 한국정밀공학회지
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• 제20권2호
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• pp.94-103
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• 2003

In case of environment requiring safety such as human body and requiring flexible shape, a conventional mechanical actuator system does not satisfy requirements. Therefore, in order to solve these problems. a research of various smart material such as EAP (Electro Active Polymer), EAC (Electro Active Ceramic) and SMA (Shape Memory Alloy) is in progress. Recently, the highest preferring material among various smart material is EP (Electrostictive Polymer), because it has very fast response time, powerful force and large displacement. The previous researches have been studied properties of polymer and simple control, but present researches are studied a polymer actuator. An EP (Electostrictive Polymer) actuator has properties which change variably ils shape and environmental condition. Therefore, in order to coincide with a user's purpose, it is important not only to decide a shape of actuator and mechanical design but also to investigate a efficient controller. In this paper, we constructed the control logic with an adaptive fuzzy algorithm which depends on the physical properties of EP that has a dielectric constant depending on time. It caused for a sub-actuator to operate at the same time that a sub-actuator system operation increase with a functional improvement and control efficiency improvement in each actuator, hence it becomes very important to manage it effectively and to control the sub-system which Is operated effectively. There is a limitation on the management of Main-host system which has multiple sub-system, hence it brings out the Multi-Vehicle Control process that disperse the task efficiently. Controlling the multi-dispersion system efficiently, it needs the research of Main-host system's scheduling, data interchange between sub-actuators, data interchange between Main-host system and sub-actuator system, and data communication process. Therefore in this papers, we compared the fuzzy controller with the adaptive fuzzy controller. also, we applied the scheduling method for efficient multi-control in EP Actuator and the algorithm with interchanging data, protocol design.