• Journal of Ginseng Research
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• 제38권3호
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• pp.220-225
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• 2014

An efficient in vitro protocol has been established for somatic embryogenesis and plantlet conversion of Korean wild ginseng (Panax ginseng Meyer). Wild-type and mutant adventitious roots derived from the ginseng produced calluses on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2,4-dichlorophenoxyacetic acid and 0.3 mg/L kinetin; 53.3% of the explants formed callus. Embryogenic callus proliferation and somatic embryo induction occurred on MS medium containing 0.5 mg/L 2,4-dichlorophenoxyacetic acid. The induced somatic embryos further developed to maturity on MS medium with 5 mg/L gibberellic acid, and 85% of them germinated. The germinated embryos were developed to shoots and elongated on MS medium with 5 mg/L gibberellic acid. The shoots developed into plants with well-developed taproots on one-third strength Schenk and Hildebrandt basal medium supplemented with 0.25 mg/L 1-naphthaleneacetic acid. When the plants were transferred to soil, about 30% of the regenerated plants developed into normal plants.

• 한국자원식물학회지
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• 제30권1호
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• pp.88-95
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• 2017

토양수분의 변화가 블랙쵸크베리 'Nero'의 과실 특성에 미치는 영향을 조사하여 고품질 블랙쵸크베리 재배 연구의 기초자료를 제공하고자 수행하였다. 토양수분 감소로 인한 건조스트레스는 수확기 동안 블랙쵸크베리의 과실의 과중, 당도, 안토시아닌 함량 등의 품질을 저하시켰다. 또한 건조기간이 길수록 이후 토양수분 공급시, 과실 표피 세포 크기는 감소하는 반면 아표피, 과육 세포의 크기가 증가하여 열과를 유발할 수 있다. 따라서 관수시설을 통한 일정 주기의 관수가 블랙쵸크베리 과실 생산량 및 품질 향상을 위해 필요할 것으로 생각된다.

• Plant Biotechnology Reports
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• 제5권4호
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• pp.323-329
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• 2011

MuSI, a gene that corresponds to a domain that contains the rubber elongation factor (REF), is highly homologous to many stress-related proteins in plants. Since MuSI is up-regulated in the roots of plants treated with cadmium or copper, the involvement of MuSI in cadmium tolerance was investigated in this study. Escherichia coli cells overexpressing MuSI were more resistant to Cd than wild-type cells transfected with vector alone. MuSI transgenic plants were also more resistant to Cd. MuSI transgenic tobacco plants absorbed less Cd than wild-type plants. Cd translocation from roots to shoots was reduced in the transgenic plants, thereby avoiding Cd toxicity. The number of short trichomes in the leaves of wild-type tobacco plants was increased by Cd treatment, while this was unchanged in MuSI transgenic tobacco. These results suggest that MuSI transgenic tobacco plants have enhanced tolerance to Cd via reduced Cd uptake and/or increased Cd immobilization in the roots, resulting in less Cd translocation to the shoots.

• 화훼연구
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• 제17권3호
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• pp.190-193
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• 2009

2003년 화색이 우수하고 절화장이 길며 내병성을 가지나 수량이 적은 'Vivaldi'를 모계로 절화수명과 생육이 우수하며 다수성 품종인 'Medeo'를 인공교배 하여 얻은 실생계통 12주에 대하여 2004~2005년에 1차선발을 하여 2계통을 선발하였으며, 이들을 대상으로 2006~2008년간 3차에 거쳐 특성검정을 실시한 후 최종 선발된 '원교 D1-1005'를 2008년 농작물 직무육성 신품종 선정위원회를 거쳐 'Happy Day'로 명명하였다. 'Happy Day'의 화색은 분홍색으로 RHS 칼라차트번호 41C로 나타나며, 흰가루병에 대하여 저항성인 특징을 가지고 있다. 꽃잎수는 28.8개이며 화폭은 9.9 cm로 'Nobleless'와 비슷하였다. 10a 당 년간 절화수량은 160본이며 절화장은 79.2 cm 절화경경은 6.7 mm였고 절화수명은 11.8일 정도를 나타내었다.

• 화훼연구
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• 제17권3호
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• pp.197-200
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• 2009

접목선인장 신품종 '고홍'은 진적색의 'DR' 계통을 모본으로 진적색의 '설홍'을 부본으로 2004년에 교배하여 개발되었다. 이 품종은 처음 기내에서 파종한 후 유묘를 무병삼각주에 접목하고 100 mL 시험관에 정식하여 6개월을 재배하였다. 2005년에 기외에서 접목한 후에 2008년까지 3회에 걸쳐 그 특성이 조사되었다. 구는 진적색의 편원형이며, 몸체에 8~10개의 결각이 있으며, 회색의 직립 가시를 가지고 있다. 구의 직경은 44.1 mm로 대조 품종 '설홍'에 비해 구가 크고 생육 속도도 빠르다. 지구 착생수는 평균 12.1개로 12.2개의 대조 품종 '설홍'과 유사하다.

• The Plant Pathology Journal
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• 제34권6호
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• pp.506-513
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• 2018

Clubroot is one of the most economically important diseases of the Brassicaceae family. Clubroot disease is caused by the obligate parasite Plasmodiophora brassicae, which is difficult to study because it is nonculturable in the laboratory and its races are genetically variable worldwide. In Korea, there are at least five races that belongs to four pathotype groups. A recent study conducted in Korea attempted to develop molecular markers based on ribosomal DNA polymorphism to detect P. brassicae isolates, but none of those markers was either race-specific or pathotype-specific. Our current study aimed to develop race- and isolate-specific markers by exploiting genomic sequence variations. A total of 119 markers were developed based on unique variation exists in genomic sequences of each of the races. Only 12 markers were able to detect P. brassicae strains of each isolate or race. Ycheon14 markers was specific to isolates of race 2, Yeoncheon and Hoengseong. Ycheon9 and Ycheon10 markers were specific to Yeoncheon isolate (race 2, pathotype 3), ZJ1-3, ZJ1-4 and ZJ1-5 markers were specific to Haenam2 (race 4) isolate, ZJ1-35, ZJ1-40, ZJ1-41 and ZJ1-49 markers were specific to Hoengseong isolate and ZJ1-56 and ZJ1-64 markers were specific to Pyeongchang isolate (race 4, pathotype 3). The PCR-based sequence characterized amplified region (SCAR) markers developed in this study are able to detect five Korean isolates of P. brassicae. These markers can be utilized in identifying four Korean P. brassicae isolates from different regions. Additional effort is required to develop race- and isolate-specific markers for the remaining Korean isolates.

• The Plant Pathology Journal
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• 제35권1호
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• pp.63-70
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• 2019

Winter wheat is susceptible to several fungal pathogens throughout the growing season and foliar fungicide application is one of the strategies used in the management of fungal diseases in winter wheat. However, for fungicides to be profitable, weather conditions conducive to fungal disease development should be present. To determine if winter wheat yield response to fungicide application at the flowering growth stage (Feekes 10.5.1) was related to the growing season precipitation, grain yield from fungicide treated plots was compared to non-treated plots for 19 to 30 hard red winter wheat cultivars planted at 8 site years from 2011 through 2015. At all locations, Prothioconazole + Tebuconazole or Tebuconazole alone was applied at flowering timing for the fungicide treated plots. Grain yield response (difference between treated and non-treated) ranged from 66-696 kg/ha across years and locations. Grain yield response had a positive and significant linear relationship with cumulative rainfall in May through June for the mid and top grain yield ranked cultivars ($R^2=54%$, 78%, respectively) indicating that a higher amount of accumulated rainfall in this period increased chances of getting a higher yield response from fungicide application. Cultivars treated with a fungicide had slightly higher protein content (up to 0.5%) compared to non-treated. These results indicate that application of fungicides when there is sufficient moisture in May and June may increase chances of profitability from fungicide application.

• Journal of Plant Biotechnology
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• 제45권4호
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• pp.357-363
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• 2018

The objective of this study was to investigate whether exogenous proline (Pro) could confer freezing tolerance of spinach and determine fluctuations of free amino acids in spinach leaf tissues under freeze-induced stress. Treatment with Pro (10 mM) resulted in more accumulation of Pro (~2.6-fold) in Pro-treated spinaches compared to untreated ones. These Pro-pretreated spinaches were more freezing-tolerant, showing more turgid leaves and petioles compared to untreated controls. However, when spinaches pre-treated with or without Pro were subjected to freezing, there was no significant difference in overall amino acid contents, emphasizing the role of Pro as an osmoprotectant. Freezing stress prompted intensification of total amino acid contents irrespective of pretreatment with Pro. Asp, Glu, Ala, and Val were the most abundant free amino acids due to increased protein degradation and nitrogen mobilization for plant survival under freezing stress. Arg, a precursor for the synthesis of polyamines in plants, was profoundly enhanced under freezing stress. This implies that Arg plays an important role in modulating freezing tolerance. Gly, Leu, and Ile were maintained at relatively low levels in all treatments. However, Ser, Tyr, and Lys as primary constituents of dehydrins were accumulated under freezing stress, suggesting that they might play a role in increasing cryoprotective activity under freezing stress.

• 한국환경과학회지
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• 제30권12호
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• pp.1041-1051
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• 2021

The purpose of this study was to identify for extenders, adhesives, colorants, and fungicides suitable for the production of high-quality film-coated seeds of Chinese cabbage. In addition, the effect of various additives used for film coating on the germination of coated seeds and seedling vigor was investigated to establish appropriate treatment conditions for film coating efficiency. The germination rate and germination speed in Chinese cabbage film-coated seeds did not differ significantly depending on the type and concentration of the extender, while the use of Talc resulted in superior granulation capacity and adhesion of the seed coat in comparison to Zeolite. There was no significant difference in the germination rate depending on the type of adhesive used for film coating of Chinese cabbage seeds, but the germination rate was slightly slower in the film-coated seeds using carboxymethyl cellulose as an adhesive. On the other hand, the seeds coated with a film using 1% PVA as an adhesive not only maintained hardness enough to withstand external pressure, but also showed a high germination rate. In addition, the suitable disinfectant for film coating was venoram, and the treatment concentration was good at 250 mg·L^-1. The type of colorant used in the film-coated seeds of Chinese cabbage did not significantly affect the germination rate, germination speed, and seedling vigor. The seeds film-coated using the liquid colorant were superior in appearance to the seeds film-coated with the solid colorant, which implies the possibility of reduction in the manufacturing costs by using the water-soluble paints as colorants for film-coated seeds.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 춘계학술대회
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• pp.28-28
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• 2020

본 연구는 식용, 목재용, 가구용 등 유용한 상수리나무의 효율적인 양묘를 위한 기초 정보를 제공하기 위하여 용기의 종류에 따른 유묘 생장을 측정하고 조사하였다. 용기별 유묘의 엽수는 15구가 18.6개로서 24구의 18.5개보다 많았고, 엽건물중은 15구가 2.41g으로서 24구의 2.24g보다 가벼웠다. 줄기길이는 15구가 33.5cm로서 24구의 34.6cm보다 짧았고, 줄기직경은 15구가 4.48mm로서 24구의 4.77mm보다 좁았으며, 줄기건물중은 15구가 1.40g으로서 24구의 1.53g보다 가벼웠다. 뿌리길이는 15구가 39.2cm로서 24구의 49.1cm보다 짧았고, 뿌리건물중은 15구가 7.60g으로서 24구의 6.92g보다 무거웠다. 그리고 식물체건물중은 15구가 11.4g으로서 24구의 10.7g보다 무거웠다. 지상부와 지하부 (S/R) 비율은 15구가 0.50으로서 24구의 0.55보다 낮았다. 이와 같이 재배 용기의 크기에 따른 상수리나무 유묘의 생장은 여러 생장 측정 중 뿌리길이를 제외하면 T-검정에서 차이를 나타내지 않았다. 이렇게 보면 상수리나무는 용기 크기와 유묘 생장의 상관관계가 없다고 할 수 있으나 본 실험에서 노천매장 종자의 파종상 유묘를 선별하여 이식할 때 물리적 손상이 있을 수 있어 추후 추가적인 보완 실험이 필요하였다.