• 제목/요약/키워드: honeybee

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천안·아산지역 양봉농가 꿀벌질병 감염률 조사 (Prevalence of honeybee (Apis mellifera) disease in Cheonan-Asan areas, Korea)

  • 전동민;김선희;육심용;염남희;도진영;송서영;허은진;신창호
    • 한국동물위생학회지
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    • 제36권2호
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    • pp.147-150
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    • 2013
  • This study was carried out to investigate the prevalence of honeybee (Apis mellifera) disease in cheonan and asan area. From September to November in 2012, 33 samples were collected from 33 apiculture farms in the regions and reverse transcriptase-polymerase chain reaction (RT-PCR) and polymerase chain reaction (PCR) was conducted. Among 33 samples, prevalence rate was 42% in Sac Brood Virus (SBV), 52% in Nosema, 21% in American foulbrood (AFB), 70% in European foulbrood (EFB), 97% in Stonebrood, 3% in Chalkbrood. The result indicate that stonebrood was most prevalent disease in apiculture farms in cheonan and asan area.

꿀벌 Apis mellifera에서 유래 한 특성화 되지 않은 항균성 펩티드의 동정 (Identification of Uncharacterized Anti-microbial Peptides Derived from the European Honeybee)

  • 박희근;김동원;이만영;최용수
    • 생명과학회지
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    • 제30권1호
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    • pp.64-69
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    • 2020
  • 꿀벌(Apis mellifera)에는 많은 항균성 펩티드가 있습니다. 그러나 아직 많은 종류의 펩티드를 기능을 알려지지 않았다. 따라서, 알려지지 않은 기능성 펩티드의 특성화가 필요하다. 그래서 우리는 새로운 항균성 펩티드(AMP)를 분석 하였다. 우리는 Apis mellifera에서 total RNA를 분리하고 Illumina HiSeq 2500 차세대 시퀀싱(NGS) 기술을 사용하여 15,314 개의 펩티드 서열을 생성하여 새로운 AMP를 선발 하였다. AMP로서 기능을 가지는 AMP를 선발 하기 위해 AMP 서열의 특성 과 특징을 분석을 기초로 하여 알려지지 않은 펩티드 및 알려진 44 개의 펩티드가 확인 되었다. 그 중에서도 AMP5라는 특성화 되지 않은 펩티드를 선발 하였다. AMP5는 표피, 지방체, 독낭에서 발현된다. 항균 활성을 분석하기 위해 Gram-negative bacteria Escherichia coli KACC 10005 및 Bacillus thuringiensis KACC 10168에 대한 항균 활성을 합성한 AMP5 처리하여 시험 하였다. AMP5는 Gram-negative bacteria Escherichia coli에 대한 항균 활성을 나타냈다(MIC50 = 22.04±0.66 μM). 일벌에 Escherichia coli을 주사했을 때 AMP5는 체내에서 항균성 펩티드로 발현이 높아졌다. 이러한 결과는 Escherichia coli에 대한 항균 활성을 나타냄을 확인하였다.

꿀벌 외래 해충, 작은벌집밑빠진벌레(Aethina tumida Murray, 1867)의 초기 발견 봉장 내 공간 분포 특성 (Spatial Distribution Patterns of a Newly Invaded Honeybee Pest, Aethina tumida Murray, 1867 (Coleoptera: Nitidulidae) in an Apiary Where it was First Detected)

  • 홍석민;정철의
    • 한국양봉학회지
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    • 제32권3호
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    • pp.163-170
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    • 2017
  • 꼬마벌집밑빠진벌레(SHB, Aethina tumida Murray(Coleoptera : Nitidulidae))는 벌통 내부에 소비와 저장식량에 피해를 주는 꿀벌 해충이다. 국내 Apis mellifera 벌통에 대한 SHB의 최초 피해 보고는 2016년 9월 23일 경남 밀양시에서 보고되었다. 그 후, 2016년 10월 5일 밀양시 양봉장에서 벌통 내부와 벌통 입구 30cm내에서 SHB 공간 분포를 조사했습니다. 총 169개의 벌통을 조사하였다. 벌통 내부에서는 SHB 유충과 성충이 발견되었으며, 토양 내에서는 성충, 유충 그리고 번데기가 발견되었다. 성충 SHB가 발견된 벌통 중 61% 벌통 내 꿀벌이 존재하였으나, 유충 SHB가 발견된 벌통에서는 꿀벌이 존재하지 않았으며, 유충에 의한 봉군 파괴를 뜻하였다. 벌통 내 꿀벌이 존재하는 경우 꿀벌 개체수가 많을수록 SHB의 밀도가 높았다. 분산 계수(CD)는 벌통 내부와 토양 내부 간의 중요한 군집 분포를 보였다. CD는 토양 내부보다 벌통 내부에서 훨씬 높았다. SHB에 의해 꿀벌이 피해를 입어 벌통을 제대로 관리하지 못하여 벌통 내 봉군의 붕괴를 일으킨다는 것을 뜻한다. 또한 10월에도 여전히 벌통 내부에서 SHB의 발육과 번식이 이루어져 늦은 가을까지 SHB의 개체군이 증가할 수 있다. SHB의 한국환경 적응에 대한 더 자세한 분석은 꿀벌에 새롭게 발견된 신규 외래해충의 관리 전략을 제시하는데 도움이 될 수 있다.

수밀력 우수 꿀벌 계통 판별을 위한 계통 특이 분자마커 개발 (Identification of a Single Nucleotide Polymorphism (SNP) Marker for the Detection of Enhanced Honey Production in Hoenybee)

  • 김혜경;이명렬;이만영;최용수;김동원;강아랑
    • 한국양봉학회지
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    • 제32권3호
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    • pp.147-154
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    • 2017
  • 꿀벌은 화분매개 뿐만 아니라, 양봉산물을 생산하는 주요한 산업곤충 중 하나이다. 최근 농촌진흥청과 예천곤충 연구소에서는 국내 최초로 수밀력 우수 꿀벌 품종인 '장원벌'을 선발하여 보급하고 있다. 본 연구에서는 장원벌 계통 특이 분자 마커 개발을 위해 장원벌 부계인 D계통 특이적인 분자 마커를 개발 하고자 Sequence-Based Genotyping (SBG) 분석을 수행하였다. SGB 분석은 농촌진흥청 국립농업과학원에서 보존 육성중인 A, C, D, E, F 5개 기본종 계통에 대해 수행되었으며, 이를 통해 1,029개 SNP를 확보 할 수 있었다. 이후 A, C, D, F, E 기본종 계통 및 $D{\times}F$ 교배계통에 대한 SNP filtering 및 validation을 통해 최종적으로 AmD6 및 AmD9 두 개의 SNP 마커를 선발 하였으며, genotyping 분석을 통해 AmD9 마커가 장원벌 부계인 D 계통을 100% 구분 할 수 있는 것으로 확인되었다. 본 마커를 통해 D 계통 및 장원벌을 보다 정확하게 판별하고 육종에 활용 할 수 있을 것으로 기대하고 있다.

Avoidance Behavior of Honey bee, Apis mellifera from Commonly used Fungicides, Acaricides and Insecticides in Apple Orchards

  • Kang, Moonsu;Jung, Chuleui
    • 한국양봉학회지
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    • 제32권4호
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    • pp.295-302
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    • 2017
  • Avoidance behavior is an important life history strategy to survive hazardous environment. The experiment was conducted to detect the avoidance tendency of the honeybee Apis mellifera against commonly used pesticides in apple production. Choice test given only 50% sucrose solution and pesticide-mixed sucrose solution as food estimated the avoidance in laboratory. Most of the acaricides and fungicides tested were shown avoided. Among insecticides, honeybee showed strong avoidance to cyhexatine, carbosulfan and fenpyroximate but low to diflubenzuron, tebufenpyrad, and acrinathrin. Avoidance behavior to neonicotinoid insecticides showed bifurcated; highly avoided from thiacloprid, acetamiprid while less avoided from imidacloprid, thiamethoxam and dinotefuran. From the field study, abamectin, fenthion, amitraz and acequinocyl showed highly avoided while fungicide of fenarimol, acaricides of acrinathrin and phosphamidon, IGR insecticide of diflubenzuron, neonicotinoid insecticide of imidacloprid, and carbamate insecticide of carbaryl showed less avoidance in the field. These results partly explained high bee poisoning from carbaryl in apple flowering period, and neonicotinoids during season.

GM 콩의 도입유전자 이동에 미치는 화분 매개충의 영향 (Influence of insect pollinators on gene transfer from GM to non-GM soybeans)

  • 이범규;김준형;손수인;권순종;박기웅;정영수;이시명
    • 농업과학연구
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    • 제42권3호
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    • pp.159-165
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    • 2015
  • The cultivation area and use of genetically modified (GM) crops have been increased continuously over the world and concerns about the potential risks of GM crops are also increasing. One of the major concern in risk assessment is the possible development of hybrids through interspecific and intergeneric crosses with related species. This study was conducted to investigate the pollinator have an influence on insect-mediated gene transfer from GM soybeans. Hybrid was induced from GM soybeans by honeybee and western flower thrips, and non-GM soybeans were used as pollen receptor. The analysis for gene-flow was conducted by herbicide selection, immunostrip test, and PCR analysis. In the result of the analysis, three hybrids were detected on the distance 15, 75, 105 cm from pollen source in western flower thrips treatment. In honeybee treatment, one hybrid was detected in the farthest distance (300 cm). These results suggested honeybee and western flower thrips have a possibility they can transfer the introduced gene from GM soybeans to non-GM soybeans.

동.서양의 복식에 표현된 동물문양의 상징성에 관한 비교 연구 - 유교적 불교문화권과 그리스도교 문화권과의 비교를 중심으로 - (An analysis of symbols of animal patterns in oriental and occidental clothing - focusing on comparison of Buddhism- Confucianism and Christian cultural area -)

  • 이윤정
    • 한국생활과학회지
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    • 제15권3호
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    • pp.469-480
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    • 2006
  • Patterns in clothing symbolize some specific meaning that differs across cultures and religions, although patterns have been mostly used to realize human desires to overcome natural hazards in every culture or religion. This research aims to compare animal patterns of Korea (Confucian/Buddhism culture) with those of Byzantine (Christian culture), based upon a research method of literature survey. It is found through the research that both cultures used common patterns, such as dragon, butterfly, bat, honeybee, peacock, fish, and chicken. In the case of dragon patterns, Korea used it to symbolize emperor, dignity, or authority, while Byzantine used it for devil. Bat patterns had different symbolic meanings in both cultures: they mean happiness, longevity, prosperity or many off-springs in Korea while they mean misfortune or unhappiness in Byzantine. On the other hand, the rest of animal patterns, including butterfly, honeybee, peacock, chicken, had quite a similar symbolic meaning. Butterfly means change of life or beginning of new life in Korea, whereas it means resurrection in Byzantine. Honeybee symbolizes diligence, order, and cooperation. Many animal patterns were used in both cultures at the same time. Another difference can be found in terms of the kind of animals; for example, Korea used wild goose, pheasant, giraffe, deer, mandarin duck & turtle, while Byzantine used eagle, snake, pigeon. Of course, each and every animal has its own meaning, mostly positive. Except dragons and bats, both cultures have a similar meaning in most of animal patterns. Generally speaking, Korea used imaginary animals to pray for human happiness, but Byzantine used real animals to pray for resurrections.

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주사제 사용을 위한 봉독의 균질성 및 안정성 평가 (Experimental studies of homogeneity and stability honeybee venom using ultra-high performance liquid chromatography)

  • 한상미;김세건;홍인표;우순옥;장혜리;이경우
    • 한국동물위생학회지
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    • 제39권2호
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    • pp.81-86
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    • 2016
  • Honeybee venom (BV) from Apis mellifera L. has been used as natural antimicrobial compounds in pigs, cows, dairy cattle and chicken farms in Korea. The purpose of this study was conducted to confirm homogeneity and stability of BV dissolved with distilled water or saline solution. Melittin was analyzed with ultra-high performance liquid chromatography (UPLC) for BV to secure the validation of analysis. BV at concentration of 1 mg/mL was dissolved with distilled water or saline solution at room temperature. Homogeneity of BV dissolved with distilled water or saline solution at upper, milddle, and lower layers all satisfied the accuracy and precision criteria. Stability of BV dissolved with distilled water or saline solution for 7 days all satisfied the criterion both light and dark storage condition. BV has satisfied with homogeneity and stability in distilled water or saline solution at room temperature under light or dark condition. The results of this study suggest that BV has a possibility as the substitute of natural antimicrobial agents for the animal drugs and feed additives.

광주광역시 꿀벌질병 동향조사 (Prevalence of honeybee (Apis mellifera) diseases in Gwangju)

  • 이인행;김지연;최종욱;고바라다;정보람;박재성;나호명;김용환
    • 한국동물위생학회지
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    • 제41권2호
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    • pp.111-118
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    • 2018
  • This study was carried out to investigate the prevalence of honeybee (Apis mellifera) diseases in Gwangju area. From November 2016 to August 2017, 89 samples were collected from 33 apiculture farms and reverse transcriptase-polymerase chain reaction (RT-PCR), polymerase chain reaction (PCR), and real time PCR were conducted. 14 infectious pathogens, including seven viruses, two bacteria, three fungi, and two parasites, were investigated from random apiculture farms in Gwangju. The percentage of infectious pathogens were as follows: Stonebrood (76.4%), Deformed wing virus (51.7%), Nosema (27.0%) in PCR and RT-PCR. This result indicated that Stonebrood was most prevalent disease in Gwangju area. And we could get similar results from real time PCR. 84.8% of farms have more than two of infectious pathogens. Stonebrood and Deformed wing virus were major diseases in almost all seasons and Black queen cell virus disease was especially prevalent in May.

Comparative Transcriptome Analysis of Queen, Worker, and Larva of Asian Honeybee, Apis cerana

  • Kim, Woo Jin;Lee, Seok Hee;An, Saes Byeol;Kim, Song Eun;Liu, Qin;Choi, Jae Young;Je, Yeon Ho
    • International Journal of Industrial Entomology and Biomaterials
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    • 제27권2호
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    • pp.271-276
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    • 2013
  • The Asian honeybee, Apis cerana, is a native honeybee species in Korea which is important in agriculture for pollination and honey production. For better understanding of the physiology of A. cerana, high-throughput Illumina transcriptome sequencing was performed to analyze the gene expression profiles of queen, worker, and larva. A total of 219,799,682 clean reads corresponding to 22.2 Gb of nucleotide sequences was obtained from the whole body total RNA samples. The Apis mellifera reference mRNA sequence database was used to measure the gene expression level with Bowtie2 and eXpress software, and the Illumina short reads were then mapped to 11,459 out of 11,736 A. mellifera reference genes. Total of 9,221 genes with FPKM value greater than 5 of each sample group were subjected to eggNOG with BLASTX for gene ontology analysis. The differential gene expression between queen and worker, and worker and larva were analyzed to screen the overexpressed genes in each sample group. In the queen and worker sample group, total of 1,766 genes were differentially expressed with 887 and 879 genes overexpressed over two folds in queen and worker, respectively. In the worker and larva sample group, total of 1,410 genes were differentially expressed with 1,009 and 401 genes overexpressed over two folds in worker and larva, respectively.