Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
권호 표지
DOI QR코드

DOI QR Code

Prevalence of honeybee (Apis mellifera) diseases in Gwangju

광주광역시 꿀벌질병 동향조사

  • Lee, In-Haeng (Health & Environment Research Institute of Gwangju) ;
  • Kim, Ji-Yeon (Health & Environment Research Institute of Gwangju) ;
  • Choi, Jong-Uk (Health & Environment Research Institute of Gwangju) ;
  • Koh, Ba-Ra-Da (Health & Environment Research Institute of Gwangju) ;
  • Jung, Bo-Ram (Health & Environment Research Institute of Gwangju) ;
  • Park, Jae-Sung (Health & Environment Research Institute of Gwangju) ;
  • Na, Ho-Myoug (Health & Environment Research Institute of Gwangju) ;
  • Kim, Yong-Hwan (Health & Environment Research Institute of Gwangju)
  • 이인행 (광주광역시 보건환경연구원) ;
  • 김지연 (광주광역시 보건환경연구원) ;
  • 최종욱 (광주광역시 보건환경연구원) ;
  • 고바라다 (광주광역시 보건환경연구원) ;
  • 정보람 (광주광역시 보건환경연구원) ;
  • 박재성 (광주광역시 보건환경연구원) ;
  • 나호명 (광주광역시 보건환경연구원) ;
  • 김용환 (광주광역시 보건환경연구원)
  • Received : 2018.03.30
  • Accepted : 2018.06.18
  • Published : 2018.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to investigate the prevalence of honeybee (Apis mellifera) diseases in Gwangju area. From November 2016 to August 2017, 89 samples were collected from 33 apiculture farms and reverse transcriptase-polymerase chain reaction (RT-PCR), polymerase chain reaction (PCR), and real time PCR were conducted. 14 infectious pathogens, including seven viruses, two bacteria, three fungi, and two parasites, were investigated from random apiculture farms in Gwangju. The percentage of infectious pathogens were as follows: Stonebrood (76.4%), Deformed wing virus (51.7%), Nosema (27.0%) in PCR and RT-PCR. This result indicated that Stonebrood was most prevalent disease in Gwangju area. And we could get similar results from real time PCR. 84.8% of farms have more than two of infectious pathogens. Stonebrood and Deformed wing virus were major diseases in almost all seasons and Black queen cell virus disease was especially prevalent in May.

Keywords

References

  1. 유미선, 이도부, 하정순, 이혜민, 윤병수. 2009. 꿀벌 질병 검출을 위한 real-time PCR 진단법의 개발. 한국양봉학회학술대회 자료집. 33-3.
  2. 현방훈, 조윤상, 유미선, 한도현, 서현지, 이명렬, 최용수 김혜경. 2017. 작은벌집딱정벌레 감염증 발생. pp. 6-8. 2017 작은벌집딱정벌레 예방 및 관리. 농림축산검역본부, 농촌진흥청, 국립농업과학원 공동발행.
  3. Allen MF, Ball BV. 1996. The incidence and world distribution of the honey bee viruses. Bee World. 77: 141-162. https://doi.org/10.1080/0005772X.1996.11099306
  4. Benjeddou M. Leat N, Allsopp M, Davison S. 2001. Detection of Acute Bee Paralysis Virus and Black Queen Cell Virus from honeybees by Reverse Transcriptase PCR. Appl Env Microbiol 67: 2384-2387 https://doi.org/10.1128/AEM.67.5.2384-2387.2001
  5. Core A, Runckel C, Ivers J, Quock C, Siapno T, Denault S, Brown B, Derisi J, Smith CD, Hafernik J. 2012. A new threat to honey bees, the parasitic phorid fly Apocephalus borealis. PLoS One 7(1): e29639. https://doi.org/10.1371/journal.pone.0029639
  6. Grabensteiner W, Ritter W, Carter MJ, Davison S, Pechacker H, Kolodziejek J, Boecking O, Derakhshifar I, Moosbeckhofer R, Licek E, Nowotny N. 2001. Sacbrood virus of the honeybee (Apis mellifera): rapid identification and phylogenetic analysis using reverse trascription-PCR. Clin Diagn Lab Immunol 8: 93-104.
  7. Ha JS, Lee HM, Kim DS, Lim YK, Yoon BS. 2005. A PCR detection method of Melissococcus pluton for rapid identification of European foulbrood. Korean J Apiculture 20: 9-18.
  8. Jeon DM, Kim SH, Yook SY, Yeam NH, Do JY, Song SY, Heo EJ, Sin CH. 2013. Prevalence of honeybee (Apis mellifera) disease in Cheonan-Asan areas, Korea. Korea. Korean J Vet Serv 36: 147-150. https://doi.org/10.7853/kjvs.2013.36.2.147
  9. Kim HK, Choi YS, Lee ML, Lee MY, Lee KG, Ahn NH. 2008. Detection of sacbrood virus (SBV) from the honeybee in Korea. Korean J Apiculture 23: 103-109.
  10. Kim YJ, Kim JH, Oh YH, Lee SJ, Son SK, Joung EY, Lee SJ, Lee SJ, Mon BC. 2016. Prevalence of honeybee (Apis mellifera) disease in Daejeon, Korea. Korea. Korean J Vet Serv 39(4): 253-258. https://doi.org/10.7853/kjvs.2016.39.4.253
  11. Kojima Y, Toki T, Morimoto T, Yoshiyama M, Kimura K, Kadowaki T. 2011. Infestation of Japanese native honey bees by tracheal mite and virus from non-native European honey bees in Japan. Microb Ecol. 62(4): 895-906. https://doi.org/10.1007/s00248-011-9947-z
  12. Lee HM, Ha JS, Jo YH, Nam SH, Yoon BS. 2004. PCR detection method of Ascosphera apis, Aspergillus flavus for rapid identification of fungal disease in honeybee. Korean J apiculture 19: 139-148.
  13. Lee HM, Lee DB, Han SH, Nam SH, Lim YK, Yoon BS. 2005. Rapid identification of Ascosphera apis causing chalkbrood disease in honeybee by real-time PCR. Korean J Apiculture 20: 109-116.
  14. Liu X, ZhangY, Yan X, Han R. 2010. Prevention of Chinese sacbrood virus infection in Apis cerana using RNA interference. Curr Microbiol 61: 422-428. https://doi.org/10.1007/s00284-010-9633-2
  15. Ouh IO, Do JC, Jeong TN, Cho MH, Kwak DM. 2013. Molecular detection of infectious pathogens in honeybee colonies reared in eastern Gyeongbuk province, Korea. Korean J Vet Serv 36: 37-44. https://doi.org/10.7853/kjvs.2013.36.1.37
  16. Ribiere M, Triboulot C, Mathieu L, Aurieres C, Faucon JP, Pepin M. 2002. Molecular diagnostic of choronic bee paralysis virus infection. Apidologie 33: 339-351. https://doi.org/10.1051/apido:2002020
  17. Stoltz D, Shen XR, Boggis C, Sisson G. 1995. Molecular diagnosis of Kashmir bee virus infection. J Api Res 34: 153-160. https://doi.org/10.1080/00218839.1995.11100900
  18. Thu HT, Thi Kim Lien N, Thuy Linh M, Le TH, Hoa NT, Hong Thai P, Reddy KE, Yoo MS, Kim YH, Cho YS, Kang SW, Quyeu DV. 2016. Prevalence of bee viruses among Apis cerana populations in Vietnam. J Apic Res 55: 379-385. https://doi.org/10.1080/00218839.2016.1251193
  19. Yoo MS, Lee DW, Kim IW, Kim DS, Kwon SH, Lim YG, Yoon BS. 2007. Identification of black queen cell virus from the honeybee in Korea. korean j Apiculture 22: 43-52.
  20. Yoo MS, Yoon BS. 2009. Incidence of honeybee disease in korea 2009. Korea J Apiculture 24: 273-278.