• BMB Reports
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• 제37권4호
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• pp.408-415
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• 2004

The expression of the Paenibacillus sp. A11 cyclodextrinase (CDase) gene using the pUC 18 vector in Escherichia coli JM 109 resulted in the formation of an insoluble CDase protein in the cell debris in addition to a soluble CDase protein in the cytoplasm. Unlike the expression in Paenibacillus sp. A11, CDase was primarily observed in cytoplasm. However, by adding 0.5 M sorbitol as an osmolyte, the formation of insoluble CDase was prevented while a three-fold increase in cytoplasmic CDase activity was achieved after a 24 h-induction. The recombinant CDase protein was purified to approximately 14-fold with a 31% recovery to a specific activity of 141 units/mg protein by 40-60% ammonium sulfate precipitation, DEAE-Toyopearl 650 M, and Phenyl Sepharose CL-4B chromatography. It was homogeneous by non-denaturing and SDS-PAGE. The enzyme was a single polypeptide with a molecular weight of 80 kDa, as determined by gel filtration and SDS-PAGE. It showed the highest activity at pH 7.0 and $40^{\circ}C$. The catalytic efficiency ($k_{cat}/K_m$) values for $\alpha$-, $\beta$-, and $\gamma$-CD were $3.0{\times}10^5$, $8.8{\times}10^5$, and $5.5{\times}10^5\;M^{-1}\;min^{-1}$, respectively. The enzyme hydrolyzed CDs and linear maltooligosaccharides to yield maltose and glucose with less amounts of maltotriose and maltotetraose. The rates of hydrolysis for polysaccharides, soluble starch, and pullulan were very low. The cloned CDase was strongly inactivated by N-bromosuccinimide and diethylpyrocarbonate, but activated by dithiothreitol. A comparison of the biochemical properties of the CDases from Paenibacillus sp. A11 and E. coli transformant (pJK 555) indicates that they were almost identical.

• Advances in concrete construction
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• 제4권2호
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• pp.71-88
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• 2016

Metakaolin, a dehydroxylated product of the mineral kaolinite, is one of the most valuable admixtures for high-performance concrete applications, including constructing reinforced concrete bridges and impact- and fire-resistant structures. Concretes produced using metakaolin become more homogeneous and denser compared to normal-strength concrete. Yet, these changes cause a change of volume throughout hardening, and increase the brittleness of hardened concrete significantly. In order to examine how the use of metakaolin affects the fracture and mechanical behavior of high-performance concrete we produced concretes using a range of water to binder ratio (0.42, 0.35 and 0.28) at three different weight fractions of metakaolin replacement (8%, 16% and 24%). The results showed that the rigidity of concretes increased with using 8% and 16% metakaolin, while it decreased in all series with 24% of metakaolin replacement. Similar effect has also been observed for other mechanical properties. While the peak loads in load-displacement curves of concretes decreased significantly with increasing water to binder ratio, this effect have been found to be diminished by using metakaolin. Pore structure analysis through mercury intrusion porosimetry test showed that the addition of metakaolin decreased the critical pore size of paste phases of concrete, and increasing the amount of metakaolin reduced the total porosity for the specimens with low water to binder ratios in particular. To determine the optimal values of water to binder ratio and metakaolin content in producing high-strength and high-performance concrete we applied a multi-objective optimization, where several responses were simultaneously assessed to find the best solution for each parameter.

• 여성건강간호학회지
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• 제18권2호
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• pp.149-158
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• 2012

Purpose: This study was done to test the effects of Oketani breast massage on breast pain and breast milk pH of mothers, and sucking speed of neonates. Methods: A nonequivalent control group and a pretest-posttest design was used. Postpartum mothers complaining of breast pain were recruited at a postpartum care center. The application of Oketani breast massage by an Oketani massage therapist was the experimental treatment. The control group received the conventional massage technique from a nurse at the postpartum care centre. The collected data were analysed using a $x^2$-test and a t-test with the SPSS WIN 12.0 program. Results: The participants were homogeneous in age, gestation period, and birth weight. Breast pain (t=8.384, p<.001) was significantly relieved, and breast milk pH (t=4.793, p<.001) was significantly increased in the experimental group compared to the control group. The sucking speed of the neonates in the experimental group was significantly increased compared to the control group (t=9.920, p<.001). Conclusion: These findings indicate that Oketani breast massage is effective in relieving breast pain and increasing breast milk pH as well as the sucking speed of neonates.

• 한국식품과학회지
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• 제21권5호
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• pp.606-612
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• 1989

한국산 바지락으로부터 새로운 렉틴을 아세톤파우더, 황산암모늄 침전, 친화력 크로마토그라피 및 FPLC의 이온교환 크로마토그라피법으로 분리 정제하였다. 이 렉틴은 사람의 적혈구를 비특이적으로 응집시켰으며, 생쥐와 토끼의 적혈구 및 생쥐의 복수 Sarcoma 180 세포를 응집시키지 않았고 사람의 말초혈관 임파구도 분열 촉진시키지 못하였다. 전기영동상에서 하나의 주된 띠로 나타났으며 분자량은 Biogel P-300겔 여과에서 131,000, SDS 전기영동상에서는 125,000으로 나타났다. Subunit는 33,000과 30,000의 다른 폴리펩타이드로 tetramer로 추정된다. EDTA에 의해서 활성이 저해된 바지락 렉틴은 $Ca^{++}$과 $Mn^{++}$에 의하여 적혈구 응집력이 회복되었다. 또한 이 렉틴은 약 4.2% 중성당을 함유한 당단백질임이 확인되었다.

• 한국재료학회지
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• 제25권8호
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• pp.386-390
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• 2015

Fe-base superalloy powders with $Y_2O_3$ dispersion were prepared by high energy ball milling, followed by spark plasma sintering for consolidation. High-purity elemental powders with different Fe powder sizes of 24 and 50 mm were used for the preparation of $Fe-20Cr-4.5Al-0.5Ti-O.5Y_2O_3$ powder mixtures (wt%). The milling process of the powders was carried out in a horizontal rotary ball mill using a stainless steel vial and balls. The milling times of 1 to 5 h by constant operation (350 rpm, ball-to-powder ratio of 30:1 in weight) or cycle operation (1300 rpm for 4 min and 900 rpm for 1 min, 15:1) were applied. Microstructural observation revealed that the crystalline size of Fe decreased with an increase in milling time by cyclic operation and was about 15 nm after 3 h, forming a FeCr alloy phase. The cyclic operation had an advantage over constant milling in that a smaller-agglomerated structure was obtained. The milled powders were sintered at $1100^{\circ}C$ for 30 min in vacuum. With an increase in milling time, the sintered specimen showed a more homogeneous microstructure. In addition, a homogenous distribution of Y-compound particles in the grain boundary was confirmed by EDX analysis.

• 자원리싸이클링
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• 제4권1호
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• pp.4-11
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• 1995

국내 각 채석장 및 석재 가공공정에서 발생되는 폐석과 석분 슬러지를 활용하여 장식재, 바닥재 및 내외장재 등에 쓰이는 인조 석판재 제조기술을 개발하고자 하였다. 인조석재는 폐석과 결합제 및 경화제 등을 혼합하여 제조하였으며 성형압력, 폐석 및 석분 슬러지의 결합비 그리고 결합제의 양 등의 변화에 따른 인조석재 각각의 특성을 비교하였다. 천연석재와 인조석재와의 물성을 비교하기 위하여 $\circled1$ 비중,$\circled2$ 흡수율, $\circled3$ 탄성파속도, $\circled4$ 압축강도, $\circled5$ 인장강도, $\circled6$ 반발경도, $\circled7$ 탄성계수 $\circled8$ 포아송비를 측정하였으며 $\circled9$ 내열성도 함께 조사하였는데 석재의 물성은 원료의 혼합정도, 성형압력 및 결합제의 양에 의해 좌우됨을 알 수 있었다. 본 인조석재의 제조에도 석분에 비해 고가인 결합제의 사용을 가능한한 최소로 하여 폐기물로 배출되는 폐석 및 석분슬러지를 최대한 활용할 수 있도록 하였으며 성형압력 $200kg/\textrm{cm}^2$, 결합제의 양 12~15wt.%의 제조조건에서 원하는 물성을 갖는 인조석재를 제조할 수 있었다.

• 한국식품영양과학회지
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• 제12권4호
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• pp.387-394
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• 1983

zymolyase(${\beta}-1$, 3-glucanase)의 효모(酵母) 세포벽(細胞壁)에 대한 용해작용(溶解作用)을 촉진(促進)시키는 인자(因子)를 zymolyase의 조효소(粗酵素)로부터 분리(分離)하여 Sephadex G-75 gel 여과(濾過) 및 조제용(調製用) PAG-전기영동법(電氣泳動法)에 의하여 전기영동적(電氣泳動的)으로 균일(均一)하게 정제(精製)하였다. SDS-PAG 전기영동법(電氣泳動法)에 의하여 측정한 정제(精製) 인자(因子)의 분자량(分子量)은 40,500이었고, 등전점(等電點)은 pH 9.6이었다. 정제(精製)된 촉진인자(促進因子)는 395개(個)의 아미노산 잔기(殘基)로 구성(構成)되는 단일(單一) polypeptide의 염기성(鹽基性) protease임이 밝혀졌으며, 이 protease의 흡광계수(吸光係數)($E_{208,cm}^{1%}$)는 11.9, 분자흡광계수(分子吸光係數)(${\varepsilon}_{280,}mole/l$)는 $4.83{\times}10^4$이었다.

• Journal of the Korean Physical Society
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• 제73권9호
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• pp.1340-1345
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• 2018

Mo-10 at.% Cu nanocomposite powders were fabricated by using planetary ball-milling (PBM), a mechanical alloying technique for preparing nanocomposite alloy powders of metals with mutual insolubility, and the variations in the physical and the chemical characteristics with the process conditions were investigated. We observed that Mo-10 at.% Cu was an appropriate composition to ensure a good alloying grade and minimal welding between particles. The influences of the temperature and the milling conditions on the mechanical alloying process and the phase change of Mo-10 at.% Cu composite powders were investigated, and the particle and the grain sizes of the powders after mechanical alloying were confirmed. The Mo-10 at.% Cu powders showed homogeneous elemental distributions and no phase changes up to $1200^{\circ}C$; their compositions were retained after the mechanical alloying process. The finest grain size obtained was about 5 nm for powders processed using optimum PBM processing conditions: ball-to-powder weight ratio of 5 : 1, ambient air atmosphere, a milling time of 20 h, a rotation speed of 200 rpm, and a stearic acid content of 4 wt.% produced superfine-grained Mo-10 at.% Cu nanocomposite powders with an average grain size of 5 nm (which is smaller than that of other similar materials reported in the literature). The analytical results confirmed that the PBM technique presented here is a promising method for preparing superfine-grained Mo-10 at.% Cu powders with improved properties.

• Computers and Concrete
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• 제29권 6호
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• pp.393-405
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• 2022

Lightweight concrete is a superior material due to its light weight and high strength. There however remain significant lacunae in engineering knowledge with regards to shear failure of lightweight fiber reinforced concrete beams. The main aim of the present study is to investigate the optimum usage of steel fibers in lightweight fiber reinforced concrete (LWFRC). Multi-scale finite element model calibrated with experimental results is developed to study the effect of steel fibers on the mechanical properties of LWFRC beams. To decrease the amount of steel fibers, it is preferred to reinforce only the middle section of the LWFRC beams, where the flexural stresses are higher. For numerical simulation, a multi-scale finite element model was developed. The cement matrix was modeled as homogeneous and uniform material and both steel fibers and lightweight coarse aggregates were randomly distributed within the matrix. Considering more realistic assumptions, the bonding between fibers and cement matrix was considered with the Cohesive Zone Model (CZM) and its parameters were determined using the model update method. Furthermore, conformity of Load-Crack Mouth Opening Displacement (CMOD) curves obtained from numerical modeling and experimental test results of notched beams under center-point loading tests were investigated. Validating the finite element model results with experimental tests, the effects of fibers' volume fraction, and the length of the reinforced middle section, on flexural and residual strengths of LWFRC, were studied. Results indicate that using steel fibers in a specified length of the concrete beam with high flexural stresses, and considerable savings can be achieved in using steel fibers. Reducing the length of the reinforced middle section from 50 to 30 cm in specimens containing 10 kg/m3 of steel fibers, resulting in a considerable decrease of the used steel fibers by four times, whereas only a 7% reduction in bearing capacity was observed. Therefore, determining an appropriate length of the reinforced middle section is an essential parameter in reducing fibers, usage leading to more affordable construction costs.

• 멤브레인
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• 제28권1호
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• pp.21-30
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• 2018

본 연구에서는 sol-gel법을 이용하여 균일하고 결함을 최소화한 세라믹 한외여과막을 제조하였다. 알루미나 중공사 정밀여과막 지지체의 기공 크기를 줄이기 위해, 합성된 boehmite sol과 sol-ethanol 혼합 용액을 사용하여 dip coating법으로 지지체 표면 위에 ${\gamma}$-알루미나 활성층을 형성시켰다. pristine sol을 이용하여 4회 이상 코팅했을 경우 박막층의 두께가 상당히 증가하여 균열 및 박리현상을 야기시키고, 3회 코팅을 진행하였을 경우 표면 결함이 최소화된 최적의 시료를 얻을 수 있었다. 또한, 소결 온도가 ${\gamma}$-알루미나 활성층의 기공 크기에 미치는 영향을 분석하였다. 소결 온도가 $1000^{\circ}C$일 때 가장 높은 순수 투과도 값을 보였으며, 10 nm 크기의 dextran 분획분자량(molecular weight cut-off (MWCO) : 51 kDa)이 관찰되었다. 600, $800^{\circ}C$에서 소결한 막의 경우, 12 kDa MWCO (dextran 5 nm)를 갖는 것으로 확인되었다. 결과적으로, 코팅 용액의 조성은 박막층의 두께에 큰 영향을 주었고, 소결 온도에 따라 분획분자량이 크게 영향을 받는 것을 알 수 있었다.