• 한국축산식품학회지
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• 제35권6호
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• pp.731-737
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• 2015

This study was conducted to compare the meat qualities of loin (m. longissimus dorsi) and top round (m. semimembranosus) from Hanwoo and Holstein veal. Ten Hanwoo and Holstein calves were randomly selected from a local cattle farm and raised. They were slaughtered when they were 8 mon old and weighed. Weight and percentage in primal cuts and slaughter performance of Hanwoo and Holstein veal calves are obtained. Immediately after weighting, slices of loin and top round muscles were sampled. After vacuum packaging, the samples were subjected to proximate composition, physicochemical and microbiological analyses. Dressing weight and percentage were heavier and greater (p<0.05) in the Holstein than in the Hanwoo. Water contents of the top round muscle was higher in the Holstein than in the Hanwoo (p<0.05). Water-holding capacity, protein content and CIE L* (lightness) of both muscles were higher in the Holstein than in the Hanwoo veal, whereas fat content, pH, cooking loss, a* (redness), and b* (yellowness) were higher in the Hanwoo than in the Holstein veal (p<0.05). Thiobarbituric acid and volatile basic nitrogen values of both the muscles were lower in the Hanwoo than in the Holstein veal during the first 10 d of storage (p<0.05).

• 대한수의사회지
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• 제15권9_10호
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• pp.505-510
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• 1979

At a farm raising 21 Holstein calves for meat production, 6 calves had died for 21 days with symptoms of diarrhea, inappetence, depression, dehydration and temporary rise in body temperature. Among died calves, 3 were tested pathologically and bacteriolog

• 한국유기농업학회지
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• 제20권4호
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• pp.577-587
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• 2012

본 실험은 허브부식토 및 비타민 C 공급에 따른 송아지의 면역력, 증체 및 건강에 미치는 영향을 조사하기 위하여 대조구, 허브부식토제제 첨가구(T1), 비타민C제제 첨가구(T2), 비타민 C제제와 허브부식토제제의 50 : 50 혼합 첨가구(T3)로 나누고 시험축은 각 시험구당 5두씩 임의 배치하여 수행하였다. 조사항목으로 혈중 대사물질, 사료섭취량, 체중 및 설사빈도를 측정하였다. 혈액성상으로 WBC, NE, LY 및 RBC은 처리간 유의한 차이를 나타내지 않았으나 증체율 및 사료효율은 허브 부식토를 급여한 T1구에서 다른 처리구와 비교하여 유의하게 가장 높았으며, 설사빈도는 허브 부식토+비타민 C 급여한 T3구에서 낮았지만 유의한 차이는 없었다. 따라서 본 연구에서의 Holstein 송아지에게 부식토 급여는 송아지의 면역성 향상에는 분명한 효과를 보이지 못하였으나 증체율과 사료효율에 있어 긍정적인 효과가 있는 것으로 판단되었다.

• Journal of Animal Science and Technology
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• 제52권3호
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• pp.229-236
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• 2010

Effect of molasses addition to complete calf starter (CCS) and the following pellet processing was evaluated with 12 Holstein-Friesian (HF) calves (7-14 d old with $42\;{\pm}\;5.5\;kg$ average BW), with 6 calves (replicate) per treatment. The CCS was formulated with 65% concentrate calf starter and 35% local corn fodder. On the CCS, molasses was added either 5% (M5) or 10% (M10), then the mixture was pelleted and fed to the calves for 7 weeks. Molasses addition, regardless of addition level, improved (p<0.05) both the durability and hardness of the pellet which contains 35% of high fibrous local corn fodder. Upon feeding to calves, the feed intake and daily gain were numerically higher with 5% molasses addition compared to 10% molasses addition. Blood VFA level was remarkably higher (p<0.01) in calves fed M10 than calves fed M5. There was no difference (p>0.05) in blood glucose level between M5 and M10. Length and number of papillae were not different (p>0.05) by the addition levels of molasses. However, there was one exception in number of papillae at caudo-dorsal sac which were higher (p<0.05) number in M5 than M10 calves. Considering the quality of CCS pellet, calf performance and rumen development, there was no additional benefit of 10% molasses addition compared to 5%. From the results of this study, 5% molasses addition could be recommended for local farmer when they utilize local corn fodder to make CCS for HF calves in Indonesia.

• Journal of Animal Science and Technology
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• 제51권4호
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• pp.321-328
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• 2009

본 연구는 초유 급여를 마친 갓 태어난 Holstein 송아지 14두를 대조구(Con) 및 시험구(IgY)로 나누어 49일 동안 대용유를 급여하면서 난황항체(IgY구)를 1주일 동안 투여하여 사양성적, 분변 상태 및 미생물 균총, 혈액내 생화학적 성분 및 면역관련 지표인 백혈구 성상을 조사하여 가장 바람직한 송아지 포유 및 육성 방법을 조사하였다. 시험결과로서 포유 송아지에게 IgY 투여시 체중 및 증체는 대조구(Con) 및 시험구(IgY)간에 유의적 차이 없이 모두 비슷한 성적을 보였다. 송아지 분변의 대장균(E. coli) 및 유산균 총균수를 조사한 결과 IgY 투여에 따른 대장균 및 유산균 수의 차이가 나타나지 않았으나, 분의 상태는 시험구에서 7주째 유의적으로(P<0.05) 고형상태로 배설되었다. 송아지 혈액의 백혈구 감별계수(%)를 조사한 호중구, 림프구, 단핵구, 호염기구 등의 상대적 %는 모든 구에서 정상적인 백혈구 감별 계수를 보여 IgY 투여에 따른 면역 반응에는 차이가 없는 것으로 관찰되었다. 또한 혈액내 존재하는 총 단백질, albumin 및 IgG 등에서도 대조구 및 시험구 모두 비슷한 수준을 나타내었다. 결론적으로 포유 송아지에서 1주 동안 IgY 투여는 증체, 면역작용 및 분변 미생물의 균총에는 특이적 영향이 없는 것으로 사료되어, 송아지에게 생후 24시간 이내 초유를 정확하게 급여하고 사양관리에 충실 할 경우 송아지의 포유 및 육성은 성공적으로 이루어 질 수 있을 것으로 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제25권9호
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• pp.1255-1261
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• 2012

Thirty two Holstein female calves (initial body weight = $40{\pm}3.0$ kg) were used to investigate the effects of probiotic and prebiotic on average daily gain (ADG), fecal E. coli count, white blood cell count, plasma IgG1 level and cell-mediated immune response to injection of phytohemagglutinin in suckling female calves. Calves were assigned randomly to one of the four treatments, including whole milk without additives (control), whole milk containing probiotic, whole milk containing prebiotic and whole milk containing probiotic and prebiotic (synbiotic). Average daily gain was greater in calves fed probiotic, prebiotic and synbiotic at weeks 6, 7 and 8 (p<0.05). E. coli count was significantly lower in calves fed probiotic, prebiotic and synbiotic on d 56 (p<0.05). There was no significant difference between treatments in blood samples and cell-mediated response. This study showed that addition of probiotic, prebiotic and combination of these additives to milk enhanced ADG and reduced fecal E. coli count in preruminant calves.

• Asian-Australasian Journal of Animal Sciences
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• 제21권2호
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• pp.198-203
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• 2008

This study was conducted to compare the effects of feeding high protein and low energy milk replacer (HPR; CP 25%, ME 3.6 Mcal/kg DM) with low protein and high energy milk replacer (HPR; CP 21%, ME 4.2 Mcal/kg DM) on feed consumption, body weight (BW) gain, health and selected blood metabolites in Holstein calves during the pre-weaning period. At each feeding, each milk replacer (MR) was prepared by mixing 0.125 kg of dry MR in 1L of warm ($60^{\circ}C$) water. The calves were fed either HPR (n = 10) or HER (n = 10) using mobile plastic bottles fitted with soft rubber nipples. All calves received 1.8L diluted MR at each feeding 3 times daily during the first 4 weeks of age; feeding frequency was reduced to 2 times daily for the next 2 weeks of age and then to once daily during the last week of the experiment. Jugular blood was sampled in calves at day 7, 14, 21, 35 and 49 of age to enumerate selected metabolites. Daily MR, starter and hay intake during the pre-weaning period were similar in calves fed HPR and HER. Consumption of starter, MGH and total DM steadily increased with the age of calves. Final BW, daily BW gain and feed efficiency of calves were not affected by treatments. Serum glucose, cholesterol, creatinine were decreased (p<0.05) and blood urea N was increased (p<0.05) in calves fed HER or HPR as they grew older. Serum glucose, total protein and albumin concentrations in calves were not affected by treatments. Serum GPT and GOT concentrations were higher (p<0.05) in calves on HPR than on HER. Scouring score, days scoured, respiratory score, rectal temperature and general appearance were similar in calves fed HPR and HER. Poor general appearance (dullness and droopy ears) of calves fed either HPR or HER reflected nutritional insufficiency and stress. In conclusion, energy and protein concentrations in MR did not affect feed intake and BW gain in Holstein calves during the pre-weaning period. Poor general appearance and lower BW gain of calves compared to those reported in the literature for milk fed calves prompt a demand for further research to improve the daily nutrient supply to MR-fed calves.

• Journal of Animal Science and Technology
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• 제52권4호
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• pp.281-286
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• 2010

본 연구에서는 초유를 장기간 보관하는 동시에 유산균의 함량을 증진시킨 발효초유를 Holstein 송아지의 성장 및 설사발생에 미치는 효과를 조사하였다. 발효초유를 제조하기에 적합한 균주를 선택하기 위한 실험으로 L. rhamnosus와 E. faecium을 혼합하여 사용한 경우 풍미가 좋았고 대장균군의 억제가 우수하였으며 유산균의 증식도 활발하여 이 두 균을 혼합하여 사용하는 것이 초유발효에 적합하다고 판단되었다. 송아지 사양 실험 결과로서 분변 중의 대장균과 젖산균수의 조사 결과 대장균은 발효초유 20% 첨가구에서 시간 경과 후 가장 많이 감소하는 경향을 보였으며 분내 유산균은 급여 28일째 대조구에 비하여 20% 급여수준에서 균수가 유의하게 증가하였다. 발효초유 급여 후 체중변화는 28일 후 20% 첨가구의 경우 1.4 kg (7.8%)으로 가장 높은 증체효과를 보였다. 기간별 증체효과를 살펴보면 20% 첨가구의 경우 0~14일은 0.41 kg으로 각각의 실험구 중 가장 증체량이 낮게 나타났고 14~28일의 증체량은 0.87 kg으로 이 기간 중에는 일당증체량이 가장 높게 나타나 장기 급여가 효과가 있을 것으로 추정되었다. 그러나 발효초유 급여 수준에 따른 설사 지속 일수는 무첨가구는 2.7일, 5% 첨가구 2.4일, 10% 첨가구 4일, 20% 첨가구는 2.4일로서 무첨가구, 5% 첨가구, 20% 첨가구는 3일 이내에 설사가 멈추었으나 10% 첨가구의 경우 3일 이상 지속되어 발효 초유첨가에 따른 설사 방지효과는 분명히 나타나지 않는 것으로 보였다. 이상의 결과에서 발효초유 첨가가 증체에 미치는 영향은 14일 이후 발효초유첨가 수준이 높을수록 또는 급여기간이 길수록 증체효과에 영향을 끼친다고 볼 수 있고 설사방지 효과의 경우 발효초유 첨가 수준에 의해서는 별 영향을 미치지 못하는 것을 알 수 있었다.

• 대한수의학회지
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• 제39권2호
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• pp.247-256
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• 1999

A disease of young Holstein calves characterized by recurrent pneumonia, ulcerative and granulomatous stomatitis, enteritis with bacterial overgrowth, periodontitis, delayed wound healing, persistent neutrophilia and death at an early age had been originally described in 1983 and again in 1987. Most of these calves had stunted growth and a persistent, progressive neutrophilia (often exceeding 100,000/ml). By investigation of pedigrees, all of the affected calves have now been traced to a common sire and confirmed by polymerase chain reaction (PCR) diagnostic DNA testing to be homozygous carriers of a defective allele for bovine CD18. Neutrophils from these calves have several functional deficits and, most importantly, fail to adhere in a ${\beta}_2$-integrin dependent manner. The ${\beta}_2$-integrins represent a family of glycoproteins which participate in various leukocyte adhesion reactions during host defense. The presence or absence of ${\beta}_2$-integrin molecules can be demonstrated on the surface of neutrophils, monocytes and lymphocytes from normal or affected calves using specific monoclonal antibodies and flow cytometry, or by colloidal gold immunolabeling and scanning electron microscopy in backscatter mode. Deficiency of the ${\beta}_2$-integrins on all leukocyte types in Holstein calves is analogous to leukocyte adhesion deficiency (LAD) seen in humans. Neutrophils in bovine (BLAD) and human LAD patients are unable to adhere to the endothelial lining of the cardiovascular system thus interrupting egression of neutrophils into infected tissues. Other leukocytes, while still deficient in expression of the ${\beta}_2$-integrins, are still able to efficiently egress from the blood stream due to interactions of other adhesion molecules that are not as highly expressed on neutrophils. Both BLAD cattle and LAD children (who do not receive bone marrow transplants) often die at an early age as a result of the failure of neutrophils to extravasate into infected tissues. In 1991, Shuster, et $al^{27}$, identified two point mutations within the alleles encoding bovine CD18 in a Holstein calf afflicted with leukocyte adhesion deficiency. One mutation causes an aspartic acid to glycine substitution at amino acid 128 (D128G) in an extracellular region of this adhesion glycoprotein that is highly conserved (> 95% identity) between humans, cattle and mice. The other mutation is silent. Numerous calves with clinical symptoms of leukocyte adhesion deficiency have since been tested and all have been found homozygous for the D128G allele. In addition, calves homozygous far the D128G allele have been identified during widespread DNA testing in the United States. All cattle with the mutant allele are related to one bull, who through artificial insemination (A.I.), sired many calves in the 1950's and 1960's. The carrier frequency of the D128G CD18 allele among U.S. Holstein cattle had reached approximately 15% among active A.I. bulls and 8% among cows. By 1993, the organization of the dairy industry and the diagnostic test developed to genotype cattle, enabled virtually complete eradication of bovine leukocyte adhesion deficiency among current and future A.I. bulls.

• Animal Bioscience
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• 제37권6호
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• pp.1031-1040
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• 2024

Objective: This study aimed to evaluate the effect of feeding milk replacer (MR) with varying levels of lactose and the increased supply of total solids (from 750 to 960 g/d) on performance, blood metabolites, and health of Holstein male calves during the preweaning period. Methods: Forty newborn Holstein calves (10 per treatment) were blocked according to birth weight and date of birth and distributed in a randomized block design to different liquid diets: Whole milk powder (WMP) diluted to 125 g/L solids; MR with 48% lactose (48L), diluted to 125 g/L solids; MR with 53% lactose (53L), diluted to 125 g/L solids; 53L MR corrected to 160 g/L solids (16TS) by the inclusion of a solid corrector. Calves were individually housed in wood hutches, fed 6 L/d of the liquid diet, and had free water and starter concentrate access. The study lasted 56 days. Results: Liquid diet intake was higher for calves fed 16TS than for other treatments. Calves fed 16TS presented higher protein and fat intake, followed by those fed WMP and the 48L or 53L MRs. Lactose intake was higher for 16TS-fed calves, followed by 53L, 48L, and WMP-fed calves. Starter and total dry matter intake did not differ among liquid diets. The average daily gain was higher for 16TS than 48L-fed calves, with the other treatments being intermediary. The lowest feed efficiency was observed for calves fed 48L. No effects on health were observed, as well as on selected blood metabolites, except for albumin concentration, which was higher for calves fed 16TS and WMP. Conclusion: Higher total solids content (160 g/L) in MR increases nutrient intake and consequently improves the performance of dairy calves. Feeding MRs with levels of lactose up to 53% of the DM had no deleterious effect on the performance or health of the calves.