• Journal of Microbiology and Biotechnology
• /
• 제17권9호
• /
• pp.1573-1578
• /
• 2007

An increasing number of applications is being developed for the use of nanoparticles in various fields. We investigated possible toxicities of nanoparticles in cell culture and in mice. Nanoparticles tested were Zn (300 nm), Fe (100 nm), and Si (10-20, 40-50, and 90-110 nm). The cell lines used were brain, liver, stomach, and lung from humans. In the presence of nanopaticles, mitochodrial activity decreased zero to 15%. DNA contents decreased zero to 20%, and glutathione production increased zero to 15%. None of them showed a dose dependency. Plasma membrane permeability was not altered by nanoparticles. In the case of Si, different sizes of the nanoparticles did not affect cytotoxicity. The cytotoxicity was also shown to be similar in the presence of micro-sized ($45\;{\mu}m$) Si particles. Organs from mice fed with nanoparticles showed nonspecific hemorrhage, lymphocytic infiltration, and medullary congestion. A treatment with the micro-sized particle showed similar results, suggesting that the acute in vivo toxicity was not altered by nano-sized particles.

• Asian Pacific Journal of Cancer Prevention
• /
• 제16권10호
• /
• pp.4177-4183
• /
• 2015

Background: Gastric cancer is the second leading cause of cancer-related death in Asia, and the majority type is gastric adenocarcinoma (GAC). Most GAC patients die of recurrence and metastasis. Cancer stem cells (CSCs) have been thought to be responsible for the initiation, development, metastasis, and ultimately recurrence of cancer. In this study, we aimed to investigate expression and clinical significance of CSCs markers, CD133 and Lgr5, and vasculogenic mimicry (VM) in primary GAC. Materials and Methods: Specimens from 261 Chinese patients with follow-up were analyzed for CD133, Lgr5 protein expression and VM by immunohistochemical and histochemical staining. The Pearson Chi's square test was used to assess the associations among the positive staining of these markers and clinicopathological characteristics. Postoperative overall survival time was were studied by univariate and multivariate analyses. Results: In GAC tissues, positive rates of 49.0%, 38.7%, and 26.8% were obtained for CD133, Lgr5, and VM, respectively. The mean score of microvessel density (MVD) was $21.7{\pm}11.1$ in GAC tissues. There was a significantly difference between the positive and negative groups. There was a positive relationship between the VM, the expression of CD133 and Lgr5, and the score of MVD and the grades of tumor, lymph node metastasis, TNM stages (all p<0.05). The overall mean survival time of the patients with CD133, Lgr5, VM, and MVD (${\geq}22$) positive expression was lower than that of patients with negative expression. The score of MVD, positive expression of CD133 and VM were independent prognostic factors of GAC (p<0.05). Conclusions: VM, and expression of CD133, Lgr5, and the score of MVD are related to grades of tumor, lymph node metastasis, TNM stages, and overall mean survival time. It is suggested that CSCs and VM could play an important role in the evolution of GAC.

• 한국어류학회지
• /
• 제13권2호
• /
• pp.93-99
• /
• 2001

동자개과 (Bagridae) 어류에 속하는 동자개 (P. fulvidraco)와 밀자개 (L. nitidus)의 3부위 (등면, 측면 그리고 배면)의 피부 점액세포를 광학현미경하에 조직화학적인 방법으로 조사했다. 각 종의 피부에 존재하는 점액세포를 PAS, AB-PAS 그리고 HID의 염색방법을 통해 비교해 본 결과, 두 종간에 점액세포의 성분적 차이를 발견할 수 없었고, 그러나 조사 부위에서 점액세포의 위치, 형태, 크기 그리고 수에 있어서 차이가 존재했다. 동자개의 점액세포들은 편평상피세포층과 상피의 alarm substance cells (ASCs)층에 존재했다. 그러나 밀자개의 점액세포들은 편평상피세포층에만 존재했다. 상피의 ASC 층에 존재하는 점액세포는 편평상피세포층에 존재하는 것보다 상대적으로 더 컸다. 편평상피세포층에서 두 종간 각 부위의 점액세포의 평균수는 이분산 T-test에 의해 인정되었고, 등분산 T-test에 의한 동자개의 두 층의 점액세포간의 평균수는 차이가 인정되지 않았다. 그리고 이분산 T-test에 의한 동자개와 밀자개 상피의 ASC 평균수는 등면과 배면에서만 차이가 인정되었다.