• Korean Journal of Medicinal Crop Science
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• v.24 no.4
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• pp.271-276
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• 2016

Background: In the present study, we established an HPLC (high performance liquid chromatography)-analysis method for the determination of marker compounds as a part of the material standardization for the development of health-functional foods from Salvia plebeia R. Br. extract. Methods and Results: The quantitative determination method of hispidulin as a marker compound was optimized by HPLC analysis using a YMC hydrosphere C18 column with a gradient elution system. This method was validated using specificity, linearity, accuracy, and precision tests. It showed a high linearity in the calibration curve with a coefficient of correlation ($r^2$) of 0.999995. The method was fully validated, and was sensitive, with the limit of detection (LOD) at $0.09{\mu}g{\cdot}m{\ell}^{-1}$ and limit of quantification (LOQ) at $0.27{\mu}g{\cdot}m{\ell}^{-1}$. The relative standard deviation (RSD) values of the data from intra- and inter-day precision were 0.05 - 0.22% and 0.32 - 0.42%, respectively, and the intra- and inter-day accuracy of hispidulin were 99.5 - 102.3% and 98.8 - 101.5%, respectively. The average content of hispidulin in Salvia plebeia R. Br. extract was $3.945mg{\cdot}g^{-1}$ (0.39%). Conclusions: These results suggest that the developed HPLC method is very efficient, and that it could contribute to the quality control of Salvia plebeia R. Br. extracts as a functional ingredient in health functional foods.

• Natural Product Sciences
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• v.23 no.4
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• pp.281-285
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• 2017

The purpose of this study was to confirm the anti-tumor activity of an ethanol extract of Saussurea laniceps against pancreatic tumor and to isolate the active compound from S. laniceps extract. Treatment with S. laniceps extract and hispidulin inhibited proliferation of pancreatic cell lines, such as Capan-1, Capan-2, Panc-1 and S2-013 in a dose-dependent manner using the hollow fiber assay. Hispidulin showed typical hallmarks of apoptotic cell death a significant anti-tumor activity on Capan-2 cells at a dose of 100 mg/kg and 200 mg/kg. S. laniceps has potential cytotoxic and apoptotic effects on human pancreatic carcinoma cells. Its mechanism of action might be associated with the apoptotic cell death through DNA fragmentation.

• Korean Journal of Pharmacognosy
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• v.28 no.2
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• pp.88-92
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• 1997

The full NMR assignment of hispidulin 7-0-neohesperidoside (1) isolated from Cirsium japonicum var. ussuriense was made with the aid of 2D correlation NMR techniques such as HMQC and HMBC. To investigate detoxification of bromobenzene-induced hepatic lipid peroxidation by compound 1, hepatic lipid peroxide level and the activities of enzymes responsible for production and removal of epoxide were studied. The level of lipid peroxide elevated by bromobenzene was significantly reduced by compound 1. This compound administered daily over one week before intoxication with bromobenzene did not affect the activities of aminopyrine N-demethylase, aniline hydroxylase, glutathione S-transferase. Epoxide hydrolase activity was decreased significantly by bromobenzene, which was restored to the control level by pretreatment of persicarin. The results suggest that the bromobenzene-induced hepatic lipid peroxidation by compound 1 is reduced by enhancing the activity of epoxide hydrolase, an enzyme removing bromobenzene epoxide.

• Applied Biological Chemistry
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• v.38 no.5
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• pp.473-477
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• 1995

The dichloromethane extracts of the above ground parts of Hymenoxys brachyactis afforded three sesquiterpene lactones already reported, one new sesquiterpene lactone, biennin C and hispidulin as known toxic flavone. Structures of all compounds were established by spectroscopy and biennin C was determined as an adduct of the modified pseudoguanolide and hymenoxon by Gas Chromatograpy and MS spectrometer These sesquiterpene lactones have the same ${\alpha},{\beta}$-unsaturated functional group like that of hymenovin which has been known as major toxic constituent of important livestock poison. And biennin C is also considered as toxic compound because of toxic hymenoxon.

• Journal of Applied Biological Chemistry
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• v.61 no.3
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• pp.227-232
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• 2018

Simultaneous determination of three methoxyflavones, namely, cirsimarin (1), hispidulin (2), and cirsimaritin (3) in selected Korean thistles was performed via reversed-phase high performance liquid chromatography system. Compound 1 was present in all the thistle species examined, whereas 2 and 3 were only detected in Cirsium japonicum and C. japonicum var. maackii (CJM). The concentration of compounds 1-3 in CJM varied according to the time of harvest. Plants collected in the spring (CJMS) and fall (CJMF) had the highest contents of 3 and 1, respectively. A lower content of 2 was observed in CJMF than in CJMS. This indicates that seasonal variation affects the flavonoid content of CJM. The results of this study show that CJM is an excellent source of compounds 1-3 and it can potentially be cultivated for industrial and pharmaceutical applications involving these compounds.

• Archives of Pharmacal Research
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• v.17 no.4
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• pp.273-277
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• 1994

Six flavonoids were isolated from the aerial parts of Cirsium flavonoids were identified as apigenin, luteolin, $pectolinarigenin-7-O-{\betha}-D-glucopyranoside$, linarin, pectolinarin and $hispidulin-7-O-{\alpha}-L-rhamonopyranosyl-(1{\rightarrow}2)-{\betha}-D-glucopyranoside$ on the basis of chemical and spctral evidence.

• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.2
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• pp.242-247
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• 1997

A flavone glycoside was separated from the aerial part of Circium japonicum var ussuriense Kitamura and the effect on cardiovascular system was investigated. The cadiovascular contractility of this compound was observed in the artria and aortae from normal rats. It increased the spontancous beat in right atria and the contractile force in left atria, and caused the contraction of thoracic aortae. For the blood pressure, it had ascending effect. The effective component, which acted on stimulation and contraction in the artria and aortae of rats was fractionated with n-BuOH, separated and identified by column chromatography, UV, IR, $^1H-NMR$ and $^{13}C-NMR$. The chemical structure for this component was determined to be $hispidulin-7-{\alpha}-rhamnopyranosyl(1{\rightarrow}2)-{\beta}-D-glucopyranoside$.

• Applied Biological Chemistry
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• v.50 no.1
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• pp.53-56
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• 2007

The aerial parts of Artemisia princeps PAMPANINI were extracted with 80% aqueous MeOH and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_2$O, successively. From the EtOAc fraction, five compounds were isolated through the repeated silica gel and ODS column chromatog-raphies. They were determined as friedelin (1), ${\beta}$-amyrin (2), ${\beta}$-amyrin acetate (3), camphanediol (4) and hispidulin (5) on the basis of spectral data, respectively.

• Natural Product Sciences
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• v.16 no.4
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• pp.207-210
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• 2010

Phytochemical investigation of Salvia plebeia resulted in the isolation of nine compounds. Their structures were determined to be 6-methoxynaringenin (1), 6-methoxynaringenin-7-O-$\beta$-D-glucoside (2), hispidulin (3), homoplantaginin (4), nepetin (5), nepitrin (6), 6-hydroxyluteolin (7), caffeic acid (8) and rosmarinic acid (9) by spectroscopic analyses. 6-Methoxynaringenin (1), 6-hydroxyluteolin (7) and rosmarinic acid (9) were isolated from this plant for the first time.

• Journal of the Korean Society of Food Science and Nutrition
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• v.25 no.4
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• pp.588-592
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• 1996

The flavonoids isolated from Oenanthe javanica were investigated on the antimutagenic effect againt aflatoxin $B_1(AEB_1).$ The 0.5 and 1.0mg/plate of isorhamnetin reduced the mutagenicity of $AFB_1about$ 33 and 59%, respectively. Persicarin showed the 40 and 47% inhibition rate at Previous concentration, respectively.