• Journal of Biosystems Engineering
• /
• v.34 no.1
• /
• pp.50-56
• /
• 2009

Surface plasmon resonance (SPR) biosensor has been used to detect many biochemical reactions, because this label-free sensor has high sensitivity and rapid response. The reactions are monitored by refractive index changes of the SPR biosensor. Iprovalicarb is protective, curative, and eradicative systemic fungicide introduced by Bayer AG in 1999. It has potential far control of downy mildew infesting onion, cucumber, grape and melon, late blight infesting tomato and potato, and anthracnose infesting watermelon and pepper. It is strictly limited to the maximum residue limit. In this study, the applicability of a portable SPR biosensor (Spreeta, Texas instrument, TX, USA) to detect the iprovalicarb residue was examined. The sensor chip was adopted to detect the reaction of iprovalicarb to immobilized iprovalicarb-antibody. The binding of the iprovalicarb onto the biosensor surface was measured by change of the refractive index (RI). Characteristics of the sensor chip including specificity, sensitivity, stability, and reusability were analyzed. In calibration test for seven levels of iprovalicarb concentration (0.32 to 5,000 mg/L) with three replications, a Sigmoidal model with Hill function was obtained between relative RI value and the iprovalicarb concentration with R-square of 0.998. It took 30 minutes to complete a set of detecting assay with the SPR biosensor.

• Journal of The Korean Digital Architecture Interior Association
• /
• v.11 no.3
• /
• pp.53-59
• /
• 2011

In creative design, it is necessary to understand the characteristic of architectural design. In the world of design problem, a distinction can be made between those that are well-defined and those that are ill-defined. Well-defined problems are those for which the ends or goal, are already prescribed and apparent, their solution requires the provision of appropriate means. For ill-defined problems, on the other hand, both the ends and the means of solution are unknown at the outset of the problem solving exercise, at least in their entirety. Most of design problems is ill-defined, which is unknown at the beginning of the problem solving exercise. In order to solve the design problem, Designers take advantage of the search methods of problem space, such as global-search-methods(depth-first-methods, breath-first-methods), local-search-methods(generate and test, heuristics, hill-climbing, reasoning) and visual thinking, which is represented through sketching. Sketching is a real part of design reasoning and it does so through a special kind of visual imagery. Also in the design problem solving it have been an important means of problem exploration and solution generation. By sketching, they represent images held in the mind as well as makes graphic images which help generate mental images of entity that is being designed. The search methods of problem space and a visual thinking have been crucially considered in the architectural design. The purpose of this paper is to explore the property of design by means of the pre-existed-experiment data and literature research. The findings will help design the architectural design for more creative results.

• Proceedings of the Korean Society of Applied Pharmacology
• /
• 2003.11a
• /
• pp.108-108
• /
• 2003

The current study was performed to observe the effects of Panax ginseng (PG) and P. quinquefolia (PQ) on hemodynamics such as blood flow rate (BF), blood flow velocity (BV), heart rate (HR), systolic blood pressure (SBP) and diastolic blood pressure (DBP, and body temperature (BT) in healthy young men. After testing equality of variance, Student's t-test using PROC TTEST was examined to. prove statistical differences between control and ginseng conditions at each time point. It was found that the BF data were fluctuated by personal deviation. In order to minimize the deviation, the results obtained for 6 hrs were reconstituted after dividing them into two periods of the first half from 1 to 3 hrs and of the latter half from 3.5 to 6 hrs. And then the reconstitution data and dose-response curves were obtained. Blood flow such as BF and BV shows significant increases both two periods in the dose of PG 2.25 and PG 9.0, whereas significant decrease in the dose of PG 4.5. However, in the PQ groups, the middle dose PQ 4.5 shows the highest significant increase among the three doses. Except for PG 2.25 in HR, other doses show significant decreases both in the first half and latter half. SBP of PQ 9.0 shows only a significant decrease in the first half; on the other hand, in the latter half, PG 4.5, PG 9.0 and PQ 9.0 significantly increase SBP. In addition, DBP of PG 2.25 and PG 4.5 show significant increase in the both periods. In the BT, PQ groups show gradual decrease from PQ 2.25 to PQ 9.0; however, PG groups show differently. PG 4.5 shows significant decrease, but PG 9.0 shows a increase without statistical meanings. In summary, PG is more effective in respect to keeping homeostasis of hemodynamics.

• Journal of Ginseng Research
• /
• v.36 no.3
• /
• pp.248-255
• /
• 2012

Potential antioxidant effect of processed ginseng (sun ginseng, SG) on oxidative stress generated by tert-butyl hydroperoxide (t-BHP) was investigated in HepG2 cells. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and lactate dehydrogenase (LDH) leakage test demonstrated that SG dose-dependently prevents a loss of cell viability against t-BHP-induced oxidative stress. Also, SG treatment dose-dependently relieved the increment of activities of hepatic enzymes, such as aspartate aminotrasferase and alanine aminotransferase, and lipid peroxidation mediated by t-BHP treatment in HepG2 cells. SG increased the gene expression of antioxidant enzymes such as superoxide dismutase, catalase, and glutathione peroxidase. However, high dose of SG treatment caused decrease in mRNA level of glutathione peroxidase as compared to low dosage of SG-treated cells. The gene expression of glutathione reductase was found to be slightly increased by SG treatment. In addition, SG extract attributed its hepaprotective effect by inducing the mRNA level of bcl-2 and bcl-xL but reducing that of bax. But, the gene expression of bad showed no significant change in SG-treated HepG2 cells. These findings suggest that SG has hepatoprotective effect by showing reduction of LDH release, activities of hepatic enzymes and lipid peroxidation and regulating the gene expression of antioxidant enzymes and apoptosis-related molecules against oxdative stress caused by t-BHP in HepG2 cells.

• Proceedings of the Korean Society for the Gifted Conference
• /
• 2003.05a
• /
• pp.131-135
• /
• 2003

과학영재교육의 중요한 목표중의 하나는 과학영재들의 과학에 대한 태도를 긍정적으로 변화시키는 것이다. 특히 태도와 같은 정의적 특성은 지적 특성에 비하여 후천적이고, 학습에 의해서 변화될 가능성이 크기 때문에(Marsh, 1990) 교육적으로 중요한 의미를 가지며 학생들이 성장하여 과학에 관련된 직업이나 활동을 하는 데 매우 중요한 역할을 한다(Oliver, W Hill, Pettus, W. C. and Hedin, B. A 1990). Anderson(1981)은 과학에서의 정의적 영역을 흥미, 태도, 가치, 통제의 소재, 학문적 자아개념, 불안, 선호 등의 7가지 영역으로 나누고 이 중에서 불안요인을 학업성취 및 태도에 가장 강하게 영향을 주는 요인으로 구분한 바 있다. 이처럼 과학 관련 태도나 과학 불안도는 학생들의 과학관련 활동이나 성취에 중요한 요인이 되고 있으나 이와 관련된 연구는 극히 미미하다. 지금까지의 태도 및 불안도에 관한 연구들은 일반학생들을 대상으로 하고 있으며 과학영재들을 대상으로 한 연구는 전혀 이루어지지 않았다. 따라서 본 연구는 과학영재교육원에 다니는 194명의 과학영재들을 대상으로 하여 과학영재 수업을 받은 후 과학 관련 태도와 과학 불안도가 변하는지, 과학 관련 태도와 과학 불안도는 어떠한 상관이 있는지, 그리고 남$\square$녀 집단의 차이가 있는지를 영재수업을 받기 전과 후의 검사를 통하여 알아보았다. 본 연구에서는 과학영재들의 과학 관켠 태도를 측정하기 위해‘고등학생을 위한 과학 관련 태도’검사를 사용하였다. 이 검사는 Fraser(1981)가 개발한‘TOSRA’(Test of Science-Related Attitudes)와 Munby(1983)의‘태도 측정 도구 조사’에서 문항 선정이 이루어졌다. 적절한 단어 및 어휘, 일부 문항들의 부적절한 환경 상황 등을 고려하여 40 문항 중 21문항을 수정하거나 삭제하고 문항내적 상관이 낮은 4문항을 제거하여 최종 30문항을 제시하였다(고유곤, 1996). 이 검사의 문항 내적 신뢰도 $\alpha$ 는 0.9 이었다. 검사 문항은 4개 범주과학에 대한 태도, 과학의 사회적 의미, 과학 교과에 대한 태도, 과학적 태도 등과 각 범주의 하위 문항으로 되어 있다 과학 불안도 측정 검사 도구는 중$\square$고등학생들의 과학불안도 측정을 위해 김범기(1993)가 개발한 것을 사용하였다.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.38 no.1
• /
• pp.37-44
• /
• 2014

For testing a two-wheeled vehicle's per-charge range, this study conducted road and chassis dynamometer driving tests. Three typical road routes within Daejeon Metropolitan City were selected for the road-driving test. In the case of CVS-40 mode driving tests using a chassis dynamometer, various road-loading conditions were set. In this study, two-wheeled electric vehicles' per charge range on the road was confirmed through testing, and the range and energy consumption efficiency depending on various chassis dynamometer road load settings were measured. Then, the results of the actual road driving tests were compared with those of the chassis dynamometer driving tests, and road load settings that yielded per-charge range testing results similar to those under actual road driving conditions in the chassis dynamometer experiments were studied.

• Annals of Clinical Neurophysiology
• /
• v.15 no.2
• /
• pp.42-47
• /
• 2013

Background: Diffusion-weighted image (DWI) might be useful to predict the prognosis of acute hypoxic encephalopathy. The aim of our study was to test whether the early change and extent of DWI abnormalities can be an indicator of the clinical outcome of hypoxic encephalopathy. Methods: Forty-four patients who were diagnosed as hypoxic encephalopathy due to the cardiorespiratory arrest were retrospectively identified. Clinical variables were determined, and the DWI abnormalities were counted by four areas: cortex, subcortical white matter, cerebellum and deep grey matter, and were divided into three groups by the extent of lesions. Prognosis was classified as 'poor' (Glasgow coma scale (GSC) at 30 days after arrest <9 or death) and 'good' (GSC at 30 days after arrest ${\geq}9$). Results: GCS at day 3 (p<0.001), presence of seizure (p=0.01), and presence of lesion (p<0.001) were significantly different in prognosis, but statistically there is no association with the extent of lesions and prognosis (p=0.26). Conclusions: Presence of early DWI changes could predict the clinical outcome of hypoxic encephalopathy after cardiorespiratory arrest.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.29 no.1
• /
• pp.80-91
• /
• 2001

The purpose of this research is to provide a basic knowledge to open up for undeveloped park in process of the park policy establishment by setting up the priority of park development and valuation factor, valuation index as well as building a basic material to undeveloped neighborhood park in Daegu Metropolitan City. Thirteen urban neighborhood parks(1,841,663$m^2$) in the Daegu Metropolitan City were selected for this study. Analyzing and reviewing the previous research and literature review were conducted, and the evaluation index and analysis criteria were constructed to decide the development priority of parks. The results of this research are as follows; 1) Following to the result of the expert questionnaire test in order to estimate weight of valuation factors. It had a high score in available population, and estimated amount of the gross compensation, unpurchased land rate. On the other hand park rate of autonomous district, altitude, rate of population increase in autonomous district and a low score. 2) Generally, most f the plain type parks had a high score in the Sea level, land using pattern, an estimated amount of the gross compensation, but hill type parks had a high score the rate of green covered, park area for one person of administration building. 3) Daebong, Soosung, Dosan, 2.28 youth, Kyungnam parks were selected high priority. Soochang, Donsibong, Janggi, Dongin parks were selected middle priority. Bokhyun, Galsan, Manchon, Gusoosan parks, which were selected low priority. This research for undeveloped park is a basic research step to open up. Three many difficult things in shortage of the basic material for building valuation factor and measuring of the method measurement. The application of weight which make a decision importance among the valuation factor should have ore reasonable study and more scientific research from now because there still remains a lot of shortage of positive materials in there.

• 한국방재학회:학술대회논문집
• /
• 2008.02a
• /
• pp.413-416
• /
• 2008

Recently, the results of vital tissue test showed that nitrosodimethylamine (NDMA) as a disinfection by-product (DBP), could be regarded as a carcinogen because a tumor was observed in organs. U.S.EPA indicated 0.7 ng/L as exposure concentration of NDMA based on a risk assessment target with a lifetime cancer risk of $10^{-6}$. Several recent studies have shown that UV oxidation could remove NDMA. However, UV oxidation is uneconomical and can reform NDMA after treating. In addition, the treatment mechanism of adsorption has not been founddue to the uncertainty of NDMA pathway. In addtion, NDMA has a radioisotope $^{14}C$-labeled which can be analyzed at low concentration of NDMA by Liquid Scintillation Counter (LSC). This study has investigated NDMA determination using LSC at an extremely low range from 1 to 100 ng/L and NDMA removal by powdered activated carbon (PAC) adsorption. For $^{14}C$-NDMA by LSC, the highest correlation over 99% between count number and NDMA concentrationwas obtained with possibility of $^{14}C$-NDMA concentration up to 1 ng/L. In the presence of PAC ranging from 50 to 10,000 mg/L, $^{14}C$-NDMA was removed from 18% to 97% for Sigma-Aldrich corporation (S-A co.) and from 9% to 93% by PAC for Daejung corporation (Dj co.). Hence it was found that the removal efficiency by PAC adsorption could vary depending on PAC types from different companies. For PAC adsorption capacity of $^{14}C$-NDMA using the Freundlich isotherm, $K_f$ and 1/n of PAC from S-A co. were $2.67\times10^{-3}$ ng/mg and 1.009, while those of PAC from Dj co. were $1.30\times10^{-3}$ ng/mg and 0.994, respectively. Thus, PAC from S-A co. showed twice higher adsorption capacity than Dj co.

• The Korean Journal of Physiology and Pharmacology
• /
• v.10 no.2
• /
• pp.71-77
• /
• 2006

The goal of this study was to analyze the effects of genistein, a widely used tyrosine kinase inhibitor, on cloned Shaw-type $K^+$ currents, Kv3.1 which were stably expressed in Chinese hamster ovary (CHO) cells, using the whole-cell configuration of patch-clamp techniques. In whole-cell recordings, genistein at external concentrations from 10 to $100{\mu}M$ accelerated the rate of inactivation of Kv3.1 currents, thereby concentration-dependently reducing the current at the end of depolarizing pulse with an $IC_{50}$ value of $15.71{\pm}0.67{\mu}M$ and a Hill coefficient of $3.28{\pm}0.35$ (n=5). The time constant of activation at a 300 ms depolarizing test pulses from -80 mV to +40 mV was $1.01{\pm}0.04$ ms and $0.90{\pm}0.05$ ms (n=9) under control conditions and in the presence of $20{\mu}M$ genistein, respectively, indicating that the activation kinetics was not significantly modified by genistein. Genistein $(20{\mu}M)$ slowed the deactivation of the tail current elicited upon repolarization to -40 mV, thus inducing a crossover phenomenon. These results suggest that drug unbinding is required before Kv3.1 channels can close. Genistein-induced block was voltage-dependent, increasing in the voltage range $(-20\'mV{\sim}0\'mV)$ for channel opening, suggesting an open channel interaction. Genistein $(20{\mu}M)$ produced use-dependent block of Kv3.1 at a stimulation frequency of 1 Hz. The voltage dependence of steady-state inactivation of Kv3.1 was not changed by $20{\mu}M$ genistein. Our results indicate that genistein blocks directly Kv3.1 currents in concentration-, voltage-, time-dependent manners and the action of genistein on Kv3.1 is independent of tyrosine kinase inhibition.